Document BRR2mkMdyX6Q6jJYD73rVw1rm
AR 236 - 072]
ALGICIDAL ACTIVITY
IESTSUBS~T ~~ ANC0E 000
Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, Octanesulfonic acid)
FC-95, (CAS #
or as a component 2795-39-3).
of
FC-203.
(1-
Remarks: The 3M production lot number was not noted. The test sample
disieFtCh-y2l0e3n.e gCluycrorlenbtutiynlfeotrhmeart,io3n7.in8d5i%cawtaetseirt,is2a0%mixettuhryeleonfe1g.l3yc4o%l,P2F.O6S6,%35%
Sultone foamer, 3% 0.05% tolyltriazole.
sodium
octyl
sulfate,
0.1%
sodium
lauryl
sulfate,
and
The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOO:
000000000
Method: Standard No. 22, Relating to Swimming Pool Water Treatment
Chemicals and/or Processes (National Sanitation Foundation)
Type (test type): Acute Static
.
GLP: No
Year study performed: 1974
Species: Chlorella pyrenoidosa and Phormidium inundatum
Source: In-house cultures Inc., Northbrook, IL.
maintained
at
Industrial
Bio-test
Laboratories,
Element basis: Cell populations turbidimetric measurement.
calculated
from
hemocytometer
counts,
Exposure period: 14 days
Statistical Methods: Not given.
Analytical monitoring: Not given.
Test Conditions:
-
Algal Nutrient Medium: Allen's nutrient medium.
Stock and test solution preparation: Algal solutions prepared by blending
algae with aliquots of
100 the
mL test
of Allen's medium using substance were handled
a Waring as 100%
Blender. One mL active and diluted
with
distilled water and added to Allen's medium. One mLof adjusted cell
suspension was added to each test solution, for a final volume of 100 mL. ~~
Exposure Numberof
vessels: Flasks replicates: 1
containing
final
volume
of
100
mL.
PInhiotiraml icdelilumloading: 7.5 x 10* cells/mL for Chlorella, 12.5 x 10* cells/mL for
000574
Number of concentrations: six plus a blank control for Chlorella, five plus a
Lbilgahntkicnogn:troNlotfogriPvheonrmidium.
Subculture: Inoculated bottles subcultured to fresh medium after oneday incubation, with final readings made after 14 days inoculation. Three dilutions of the test substance were subcultured (1:5, 1:10, and 1:100).
Water Chemistry: Not given.
R0 ESOWTS 0000 Nominal concentrations: Bk control, 1:5, 1:10, 1:100, 1:150, 1:1000, and
1:10000 dilution for Chiorella pyrenoidosa study. Bk control, 1:5, 1:10, 1:100, 1:1000, and 1:10000 dilution for Phorinidium inundatum study.
Results of the Study withChlorella pyrenoidosa
Sample
Gross Appearance
Turbidity
[ [Co1 nt(d5 riluotioln)[[ngoene(Coelnor) --[[5--<2o(ab0 0sor1ba0 nce) ]|
[EN 00 [nE o | -- -- n <oo|e r-- |
[[7A0000000[geegrneen[20S20 00 |
[T7B10susbuobcuullttourree|| 7700 subculture|
nnoonnee | |----<--<000.1 01|| none |--<001T|
Results of the Study with Phorinidium inundatum
`Sample
Gross Appearance
Turbidity
[ [C5 o(diolutilonT ) | (gCoelh eorn [2(e ab0 soorobarnce0 )
_
[ [Em00 0 [E1000
nnoonnee ---- |------<<o0oo0r1 |
green
200
T0000 | [5subouttore |
greentint none
||----<5020010
|
[EF-OT0E0subeulture | Cnorne--|------<001--
Element values are based on dilutions of nominal concentrations.
000575
RSuebmsatraknsc:e TReesmtianrgkwsafsieldc.ondTuhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrheeaTnedstnot the fluorochemical proportion alone.
Conclusions 0000
Algicidal rather than algistatic action of the test substance was obtained.
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
pa0 taQualry 000
Rmeeltiahboilditfyo:llKolwiemdi.scThhreanskaimnpgl=ep3u.ritSytwudayslnaoctksprdoepsecrrliyptcihoanraacntderriezceodrdasnodftthhee
study in the
lacks analytical solution.
confirmation
of
the
amount
of
fluorochemical
proportion
RE0 FERENCES0000
Test IL at
was conducted by Industrial Bio-Test Laboratories, Inc., the request of the 3M Company, St. Paul, MN, 1974.
of
Northbrook,
o0 rWE0 R 00000000000 Last changed: 6/27/00
000576
edly
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Qle oneJ Zeon
B1I91O0 F-RTOENTSATGELaRbOoAraDtories, Inc.E22) NoRiHBROOK, ILLINOIS 60062
_
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REPORT TO
3M COMPANY
-
ALGICIDAL ACTIVITY OF
TWO SAMPLES LABELED "A" AND "B"
wn
203
JANUARY 23, 1974
:
.
IBT NO. 633-04473
>
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'
000577
Jniliistich BIO = TEST Lboralories, Ine.
5
REPORT TO
3M COMPANY
TWO SAALMGPILCEISDALLAABCETLIEVDIT"YA"OAFND "B"
::
JANUARY 23, 1974. -
IBT NO. 633-0473
I Introduction
-
At the request of 3M Company, tests were conducted to determine
)
the algicidal activity of two samples identified as "A" and "B". Two
species of algae, Chlorells pyrencidosa and Phormidium inundatam,
were used in the eveluation.
.
.
000578
InduBsItOr-iTEaSlT Laboratories, Ine.
2
bh
I. Summary
i
Two samples, identified as "A" and "B", were submitted on
November 20, 1973, by 3M Company. The algicidal test was
`
initiated on November 30, 1973, using two species of algae, Chlorella
Pyrenoidosa Wisc. 2005, Phormidium inundatam Wisc. 1093.
The test was conducted according to a method outlined and
Prepared by the National Sanitation Foundation, Standard No. 22,
Relating to Swimming Pool Water Treatment Chemicals and/or Processes. The turbidimetric determination canformed to the pro-
cedure recommended by Fitzgerald*:
A 1:100 dilution of Sample "A" or "B", effectively controlled the
)
proliferation of Chlorella sp. and Phormidium sp. for a 14-day period.
Cidal rather than static action of the candidate algacides was obtained.
*
WFiattzegreraanldd,SeGw.agPe.
WBoirokass,say11f0o-r29Al6gi(c1i96d3a)l
versus
Algistatic
Cheinicals,
=
*;
Respectfully submitted,
INDUSTRIAL BIO-TEST LABORATORIES, INC.
Report prepased by; _ Deel) Clare
GDarnoiuepl LCehaadn,erM.S.
Report approved by:
1
A.
D| aO videBaskin4 , Phe .D.ee
Re
January 23, 1974 Section Head, Microbiology
.
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000579
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3
3
I. Procedure
A. Preparation of Inoculum
}
Two species of algae, Chlorella pyrenoidosa Wisc. 2005,
and Phormidium inundatum Wise. 1093, were used as the test
organisms. Each organism was subcultured at weekly intervals in
Allen's neutrient medium for three weeks, prior to inception of the test.
The suspensions of Chlorella and Phormidium were adjusted
to contatn 7.5 x 10 and 12.5 x 10 celta/ml respectively. They were
prepared by shaking the algae growth in the flasks, to which was added
100 ml of Allen's medium and blenderized for one minute in aWaring
*
Blender. Cell populations were calculated from hemocytometer counts.
y
B. Preparation of Sample
One ml aliquots of test samples handled as 100% active were
diluted in distilled water and added to Allen's medium for a final
concentration ranging from 1:5 to 1:10,000. These concentration,
;
were achieved after the addition of 1 ml of the adjusted algae cell
suspension for a final volume of 100 ml. The numbers of Chlorella
and Phormidium cells used were 7.5 x 10% and 12.5 x 10* respectively.
Six control flasks, two per species, were also prepared. All
inoculated bottles were subcultured to fresh medium after one day
incubation. Final readings for the presence of algal growth in all
bottles were made fourteen days after inoculation.
J
000550
Jodisticl BIO -TEST Laboraloris, Inc.
4
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IV. Results
|
The results of the growth examination and turbidimetric measure-
`ment of all flasks inoculated with Chlorella pyrenocidosa and Phormidium
inundatum used for the algicidal tests are presented in Table I and
I respectively.
)
:
.
"
J
000581
Indushiok BIO-TEST Loboratonts, Ine.
5
)
TABLE I
Results of the Gross and Turbidimetric
[Examination of Chlorella pyrenoidosa
y
Algicidal Test
-
Sample "A" ete "Gross Appearance ------------ TT urbie dity t
dilution)
(color)
(absorbance
1:5 1:10
:
none none
' <o.01 <0.01
1:100 aso
none . slight greentint _
<0.01 0.03
11::110,, 000000
green reen
>2.00 >2.00
1:5 subculture 1:10 subculture
none none: +. +1
<0.01 <o0.01
1:100 subculture
none
Control
green
<0.01
:
>2.00
Sample "B"
:)
1:5
none
<0.01
1:10
none
1:100
none
<o0.01 <o0.01
1:150 1:1,000
slight green tint green
0.03 >2.00 7
1:10,000 1:5 subculture
green none
>2.00
;
<0.01
1:10 subculture
none
1:100 subculture
none
<0.01 <0.01
Control
green
~ >2.00
)
o `Ssl1o,%192,
EN , 29,
fog,
000588.
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.
'
TABLE I
Results of the: Gross and Turbidimetric
Examination of Phorinidium inundatum
Algicidal Test
-
Sample "A' (dilution)
Gross Appearance color]
`Turbidity (Absorbance]
:
1:5
none
<0.01
1:10
none
<0.01.
-
1.100
1:10,.000000
none
7 ggrreeeennttiinntt
<0.01
-
>2.00
52.00
1:5 subealtare
none
oar--
1:10 subculture
none
<0.01
C1o:n1t0r0oslubculture
gnroeneen
<>02..0010
Sample "B"
)
1:5
none
<0.01
1:10
none
<0.01
1:100
1:1, 000
none
green tint
<>02..0010
11::150,su0b0c0ulture
ngorneeen tint
| <>o2..0010
"
111:11000ssuubcbuelatlutraere Control
nnoonnee green
<< o0..0011
!
>2.00
rere----------------
000583