Document BROXVZB6zxpa1GJO1Nb2e98ZE

AR _ 07t3 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (SCUD) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fiuorochemical component of the test sample. METHOD:____________________________________________________ Method: Standard Methods (APHA), 1970 Edition Type: Acute static GLP: No Year completed: 1973 Species: Gammarus fasciatus Supplier: In-house cultures maintained at Bionomics, Inc., Wareham, MA. Analytical monitoring: Temperature, pH, conductivity and DO Exposure period: 48-hours Test organism age: 12-24 hours old (fourth instar) Statistical method: TL50 (median tolerance limit) values calculated using a linear regression equation. Test conditions: Dilution water: Well water Dilution water chemistry: Alkalinity: 35 mg/L as CaC0 3 pH: 7.1 Lighting: Not given Stock and test solution preparation: Exposure concentrations prepared by direct addition of test substance. Exposure vessels: 250 mL beakers with 200 mL exposure solution Number of replicates: 4 Number of organisms per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.1 Temperature range (0-48 hours): 21 1 C Dissolved oxygen range (0-48 hours): 5.3 - 8.9 mg/L 000556 RESULTS Nominal concentrations: Bk control, 560, 870,1000,1800, and 3200 mg/L. Element values: 48-hour EC50 = 1130 (843 -1310) mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 48-hour EC50 was determined to be 1130 mg/L with a 95% Confidence Interval of 843 to 1310 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES______________________________ __ _______________ Test was conducted by Bionomics, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1973. OTHER_____________________________________________________ Last changed: 6/27/00 000557 \ I II iI i BIOASSAY REPORT SUBMITTED TO i MINNESOTA MINING & MANUFACTURING COMPANY I ST. PAUL, MINNESOTA i ! i ACUTE TOXICITY OF 3M SAMPLES (A AND B) TO THE WATER FLEA (Daphnia magna)AND SCUD (Gammarus fasciatus) . Bionomics, Inc. 790 Main Street Wareham, Massachusetts December, 1973 000558 METHODS AND MATERIALS These investigations were performed at the aquatic invertebrate toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of the water flea, Daphnia magna and scud Gammarus fasciatus to 3M samples designated as sample numbers A and B (100% active) was evaluated under static conditions for a 48 hour experimental period. Results were, expressed as the median tolerance lim^t^CTL^Q), th nominal concentration of the test compound in water causing 50 percent mortality. The TL^q values and 95% confidence ^ intervals were calculated by converting the test concentration and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression ' #: equation. -Yt M 4 U -V Both Daphnia and Gammarus were obtained from laboratory cultures and judged to be in excellent condition. Procedures for the static bioassays adhered to the protocol described by the 1970 edition of Standard Methods (APHA). Static Daphnia arid Gammarus bioassays were conducted in 250 ml beakers with 200 ml of the experimental diluent. The diluent water was obtained from a well source and was used as such. The experimental chambers were maintained in a 000553 water bath at 21 * 1C. Five 12 * 12 hour old Daphnia were introduced into each experimental beaker and five fourth instar Gammarids were introduced into each experimental chamber for the Daphnia and Gammarus bioassays, respectively. In the Daphnia bioassays, food was added at a rate of 0.5 ml/liter after the introduction of the compound to the test vessels. Bioassays were conducted using four replicates of each concentration for both compounds (A and B) for both species. The pH of the standard diluent was 7.1 and the methyl orange alkalinity was 35 ppm as CaCO^. Dissolved oxygen values for the various test vessels for both compounds ranged from 8.9 initially to 5.3 mg/1 at the end of the test. : ^"Food was prepared by homogenizing 5 g of starter trout food and 1 g of Cero-phyl in 100 ml of water. 0005CC RESULTS The predicted 48 hour TL^q (i.e. that concentration of active compound that allows 50 percent survival to the experimental animals) and 95% confidence intervals for Gammarus and Daphnia exposed to compounds A and B are presented in Table 1. A summary of the observed mortality of Daphnia and Gammarus exposed to various concentrations of compound A is given in Table 2. The observed mortality of Daphnia and Gammarus exposed' to experimental concentrations of compound B is given in Table 3. v 000561 SUBMITTED BY: PREPARED BY: APPROVED BY: Bionomics, Inc. 790 Main Street Wareham, Mass. December, -1973 S. Krogh Derr, Ph.D. Invertebrate Toxicologist Kenneth J. Macek, Ph.D. I Director /db 00 0562 Table 1 - Acute toxicity of 3 M compounds A and B to the water PK flea (Daphnia magna) and scud (Gaxnmarus fasciatus) exposed for a 48 hour experimental period. Species 48 hour TL^Q-mg/liter Compound A Compound B o') kuHtv PicA. Daphnia magna 31.1 (23.3-41.5)c Gammarus fasciatus 15.4 (12,8-20.1) 1560.0 (1280.0-2140.0 \ 1130.0 (843.0-1310.0) aBioassay conducted at 21 * 1C, Daphnia, 12 * 12 hours old at initiation of tests. ^Bioassays Conducted at 21C - 1C, Gammarus, fourth instar at * 'i - initiation of tests. c95% confidence intervals. 000563 ' i y Table 2 - Concentrations tested and corresponding observed percent mortalities for the water flea, (Daphnia magna), and such (Gammarus fasciatus) exposed to 3M compound A for a 48 hour experimental period. Species Concentration mg/1 Water Flea (Daphnia magna) 42.0 32.0 24.0 . 12.0 10.0 7.5 Control Scud 32.0 (Gammarus fasciatus) 24.0 18.0 12.0 7.5 . Control % Mortality Observed 48 hour 100 60 0 0 0 0 0 100 70 60 50 0 0 . 't >v< j 000564 S Table 3 - Concentrations tested and corresponding observed percent mortalities for the water flea (Daphnia magna) and scud (Gammarus fasciatus) exposed to 3M compound B for a 48 hour experimental period. Species Water flea (Daphnia magna) Concentration mg/1 3200.0 1800.0 1000.0 870.0 560.0 Control % Mortality Observed 48 hour 100 45 5 0 0 0 Scud (Gammarus fasciatus) 3200.0 1800.0 1000 .0 870.0 560.0 Control 100 90. t 45<?' 40 0 0 000565