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Wildlife International, Ltd. ue
Proect Number 454-104
A48HOUR STATICPAFCOSU:TE TOXICITY TEST
"WITH THE CLADOCERAN (Daphnia magna)
ADDENDUM `WILDLIFE INTERNATIONAL, LTD. PROJECTNUMBER: 454A-104
3M LABREQUEST NO. U2723
Fy U.S. Environmental Protection Agency
Series 850 - EcologicalEffectsTest Guidelines (OPPTS Number 850.1010 `OECD Guideline 202
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Wildlife International, Ltd.
2.
ProjectNumber 454AC104.
REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9%
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SPONSOR:
3MCorporation
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SPONSOR'SREPPRESENTATIVE: Ms.SusanA. Beach. boi
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STUDY:
"7 BRClOadSoc:eranA45(-DHaopuirmSitaamtaicgAncau)teToxicity Test withthe
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Ma EANMEA, SUREDTEST
NegativeControl, 11, 1954,and387m2ga,i
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TEST DATES:
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Leno oF TEST:
Experimental St-Faebr ruat ry 16, 1999 Biological Termination ~ February 18, 1999 `ExperimentalTermination ~ February 18, 1999
48Hous
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TEST ORGANS.
"Neonate cladocerans (Daphnia magna)
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SOURCE OFTESTORGANISMS: Ea`Wsitldolnif,eMIanreyrlmaantdina2l16L0d1. Cltres
AGE OFTESTORGANISMS:
<24hoursattest tation
48-HOURECS:
S9mgaill
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95% CONFIDENCE LATS:
and 87 mgaiL
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CONNOCMEONRTTRAALTIITOYN/:MMOBLITY
2mgain
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coNOn-cODaSmERTVOEDN-:FFRCT-
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Wildlife International, Ltd. ae
CERTIFOIF ACNAALYTSIES
Prost Number 4544-104
INTERIM CERTIFICATE OFANALYSIS Revision 1(9/7/00)
Centre Analytical Laboratories COA Reference #:023-0184 3M Product: PFOS,Lot217 Referenc#e: SD-018
Purity: 86.9%
[[ AResoton peW nF BoEVriE R en Comal e--E ---- -- -- E --
-- LE Tom, Mc ICP/MS) BE NL eT Looms
Tame pl | 2 ome 452. NPootaassium?
7. Manganes
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ET e LE ee emp TEE
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rae 2. 3.
Fluoride Bromide
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7 PhSousmpeharte
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1 3. HBFBA
4. NFPA _
par
. Hydrogen z Jos 4. Sulfor 5. Fluorine
ise [iat
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B lld d: aniel | hn 0 54.
f LTLEeTle HBR ERLEee Do | 67.
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hl Ea Ba
[Loe EE]
3.
a
L Teoria vie 170% L 2. `TheoreticalValue =
3. `Theoretical Value =0%
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4. TheoreticalValue = 5.95%
4.
5. TheoreticalValue =60%
5.
<00.05490 wwihnne%% DSowmwit ng
<0s.007swiwt
<o rwed ans 0.10wthn%
2m
Raswims
wt/wt 174wt/wt % 884 wtiwt% S41 ww%
COA023-018A
Page lof3
CeIntNreTAEnaRlyItiMcalCLEabRorTaItoFriIesCCAOTAEROefFereAncNeA#:LY02S3-I0S184 DateofLastAnalysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 010C Re-assessment Date: 08/31/01
F"lPuuorriityde=, 01.0509%%+- N(sMuRmoifmmpeutriatlieism,pu1r.it9i3e%s,or1g.a4n5i%c+aLciCd/iMmpSuriimtpiuersi,ti0e.s,388.%4+1P%OAInAo,rgaric 033%)
ToPtuarliitympu=ri1t0y0f%ro-m1all3tes.t=s0=8601.39.%%09%
"Potassium is expected in this salt form and is therefore not considered an impurity.
"oPbusreirtvybedyfDorStChisissgaemnpelrea.lly not applicable to materialsof low purity. No endotherm was
"iSnuolrfguarniicn atnhieosnammepltehoadppceoanrdsit1i0onbse. cTonhveeratneidontroesSuOlt4aagnrdeehsewnecleldweitetchttehdeussuilnfgurthe doenttehremirneastuilotn, itnhtehSeeOl4eimsennottalcoannsaliydseirse,dlaenndiimnpgurciotnyf.idence to this interpretation. Based
STFA
HFBA NFPA PFPA
`HTeripftlaufolruooarcoebtuitcyarciicdacid Nonofluoropentanoic acid Pentafluoropropanoic acid
"Theoretical value calculations based on the empirical formula,CiF1rSOsK"(MW=538)
This work was conducted under EPA Good Laboratory Practice Standards (40 CER 160).
COA3-018A
Page 2003
CeIntNreTAEnaRlyItiMcalCLEabRoTraItoFriIesCCAOTAEROefFereAncNeA#:LY02S3-I0S184 LC/MS Purity Profile:
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sa
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PreparedBy: Davids. Bell
pDatee er
Scientist, Centre Analytical Laboratories
ReviewedBy: _ John _ Flahe_ rty ____
E Dateee
Laboratory Manager, Centre Analytical Laboratories
COA3-018A
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September 27, 2000
3MsM. CSoursapnorAaioBnesch 9En3v5isBounsmhenAtvaelnuLaeboratory SM Builing 3.35.09 SP.tO.PaBulo,xM3i3m3e3s1ota 55133 Dear Susan 454A10E2n,clAoSsIeAd p1l0e3sAe, fSi9nAd-A10d4d,e4n5d44a10fo5r,W4i59lAd-e10I6n,teAmSat4iAon1al0,,Li4dS.4APr-o1j0ec8t2Nnudm4b4er8s-15049-101,
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KurtR Drotar
`Senior Biologist
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