Document B937QvpR802g7vV099qywebX
Attachment A
Comments On The Water Samples Sent To Exygen and Axys Analytical Services Ltd.
Four aqueous samples,a trip blank, and a field blank were sentto Exygen Researchand to Axys Analytical Services Ltd. for the analytical determination of thirteen different perfluorinated compounds. Three different analytical methods were used,two by Exygen Researchand one by Axys Analytical Services. (Note: There is no standard analytical method for this measurement approved by any governmental agency or testing organization.) The results for perfluorooctanoate f?om these measurementsare given in the table below.
Table 1. Perfluorooctanoate concentration (rig/L) (ppt)
Sample number Exygeu 1 Exygen 2 Axys
30033 10
123
98
37.8a
3000563
ndb
nd
nd
3001815
171
190
57
5000098
nd
nd
nd
FBLK- 1
nd
nd
nd
TBLK-1
nd
nd
nd
aAverage of two measurements(36.7,38.9).
bNot detected.
The first Exygen method (Exygen 1) is loosely based on a publication reported to determine four of
the thirteen analytes(Risha et al.). The Risha method is not validated for any of the sulfkcontaining analytes or for perfluorobutanoic, perfluoropentanoic, perfluorohexanoic, perfluoroheptanoic or peffluorododecanoic acids.The processto determine the LOQ in Method 1is different from that used in the Risha paper. Exygen is now developing a new and different method to determine all thirteen
analytes.Thus the datareported here using Method 1should be considered "estimates derived from a nonvalidated method". These data are not usable for regulatory reporting purposes.
Method 2 is the method usually used by Exygen to determine perfluorooctanoate in water (viz., ground water, drinking water). The method is validated according to US FDA criteria (Guidance for
Industry). The method validation includes criteria that must be met for accuracy,precision, selectivity, sensitivity, reproducibility, and stability.
The Axys method is different from both Exygen methods in that the Axys method usesa different
volume concentration step(125: 1vs. 8:l), a different sorbent for the solid-phase extraction (mixed bed vs. Cl*), no clean-up (vs. elution with a 40% methanol solution), a different analytical column (75 mm Cl8 vs. 50 mm Cs), and different elution solvent and gradient program. The internal standardsfor quantitation and the data acquisition windows for signal accumulation are also different.
Since the reported levels are in a low quantitation range (98 to 190 ppt) and since the laboratories are using two significantly different analytical methods, interlaboratory and method variability must be determined. A round-robin study is being designed that will include the new Exygen method (when it becomesavailable and validated) and the Axys method. Until the round-robin study is complete, it
s
Ms. Eileen Murphy May lo,2006 Attachment A Page 2 of2
will not known whether the pertluorooctanoate datareported from Axys and Exygen are statistically the sameor different from eachother. Note that a recent study showed that 12 of 33 experienced laboratories engaged in determining the concentration of a 7.8ppb aqueous perfluorooctanoate standard were not able to obtain acceptable results (van Leeuwen et al.). This level (7.8 ppb) is 41 to 137 times higher in concentration than the concentrations reported above. Generally the lower the concentration, the more difficult it is to obtain good precision and accuracy (US EPA, Compendium). References
K. Risha, J. Flaherty, R Willie, W. Buck, F. Morandi, and T. Isemura, And. Chem., 77,15031508 (2005).
S. van Leeuwen, A. Karrman, A. Zammit, B. van Bavel, I. van der Veen, C, Kwadijk, J. de Boer, And G. Lindstrom, "1st Worldwide Interlaboratory Study on Perfluorinated Compound in Human and Environmental Matrices", RIVO-Netherlands Institute for Fisheries Research, Report # CO70/05, October, 2005.
"Guidance for Industry: Bioanalytical Method Validation", US FDA, May 2001. "Compendium Method TO-l S', US EPA, 2ndedition, 11.3.2, January 1999.
6
Attachment B
7
`:. Proven Results.
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Analytical Report
Flu$rochemical Characterization of Aqueous Samples `.`.j, :..., Chambers Works C8 Sampling 2106 :..
1. Exygefi,, R:..esearch Laboratory Report No. LOO08093
I.. `:,
Testing Laboratory
`...
`.., Exygen Research.
"`,i,3. 058 Research Drive
St?te College, PA 16802
`..,
~&pester
Micf-&l D. Auboin URS C$poration Barley Mill Ptaza Bldg 19 R&m 2118 Lancaster Pike And Rt 141 Wilmington, D@.;19805
PAGE1 OF5
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APFO (ngll)
PFOA (ngll)
CWK:G300331 CWK-G-3003310 CWK-E-3000563 CWK-G-300()563 CWK-G-3001815 CWK-G-300182,5 CWK-G-3000098 CWK-G-3000098 CWK-K-FBLK-I CWK-K-TBLK-I
0
"`.:.
(laborat&?' duplicate)
::.
(laboratory'Cj.uplicate)
:.,
(laboratory dipljcatk)
(laboratory
dupli&Je) `.., `.., `...
102
97.6
104
99.6
ND
ND
ND,
ND
198
190
197
189
ND
ND
ND
ND
ND
ND
ND
ND
Limit of Detection (LOD) for the procedut&;is approximately 1.2 n@L
j
Limit of Quantitation (LOQ) for the procedu&js 6.0 rig/L `...
ND - Compound not detected
`:, `...
\,
NQ - Compound detected at a level between ND < LOD < NQ < LOQ d
the $D `.,, and LOQ. `...
Result is not quantifiable.
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1
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CWK-G-300331 0 CWK-G-3000563 CWK-G-3001815 CWK-G-3000098 CWK-K-FBLK-1 CWK-K-T&K-l
"`:.., `....,, `:. `:, `5.. :., `.., `\:..
APFO (rig/L)
103 ND 198 ND ND ND
PFOA (nglL)
98.6 ND 190 ND ND ND
Limit of Detection (LOD) for the procedure is approximately 1.2 n&L . .
Limit of Quantitation (LOQ) for the pro&+$ure is 6.0 rig/L
:.,
`:.
ND - Compound not detected
`,..
`L,
NQ - Compound detected at a level betweekthe LOD and LOQ. Result
is not quantifiable.
`...
ND<LOD<NQ<LOQ
`l,`I.
Results are calculated according to the following &eria `:
if the sample and laboratory duplicate are greater than..SX LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. value is reported.
Ifthe RPD is greater than 20, the higher :., :.. `:,
If the sample and laboratory duplicate are less than 5X LO& and the absolute difference is less than
LOQ, the average value is reported. If the absolute difference.,is greater than LOQ, the higher value is
reported.
:..
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Proven Results.
6 Send Report Company: Address:
Signature/Date: Printed Name:
Sample Submittal
Pleasefax this?orrn before sending samples. Please send samples to Exygen's shipping and receiving address: 3048 Research Drive, State College, PA 16801 I-G814.272.1039l F: 814.272.1019
Turnaround time (TAT) requirements:
Results Due Date:
Preliminary Results Formak 0 Verbal 0 Email Report Due Date:
Q Fax
1Storage conditions
Ci Rook temperature Q Refrigerator 0 Freezer 0 Ultra-Low freezer Cl Desiccated $ Lighting required
Stability ("C/%RH):
II& tie& &.?w
Stability time period:
Safety information
Special handling: 0 MSDS attached a Controlled substance:
tl HAZARDS:
Pleazelll in the diamond
HMWNFPA (O-4) if appropriate
Notes:
OO.14 .
Administrative Form
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`.., `.., `.., l. `..,
Analytical Report
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Fl&ochemical 1,
Characterization of Aqueous Samples
"."..C..h..a. mbers Works PWS Sampling 02/06
ExygiQ 1R, esearch Laboratory Report `...:,, `.., :.`I, Testing Laboiyifoty `:. Exygen Research
No. LO007648
"`... 3058 Research Drive
@ate College, PA 16801 -i`.:.
@equester
MichAel D. Aucoin URS @rporation Barley lU.i;lPrillaza Bldg 19 Rdom 2118 Lancaster Pike @d Rt 141 Wilmington, DE.,,l9805
PAGE f Of=5
Drive PA 16801, USA
: 8002813219 E 614.272.lU19
exygetwom
CH :.., Proven Results. `,.,
`..
Analytical
Res&~
Chambers :.. :.,
Works
PWS Sampling 2106
DuPont Sample lde&jfication -..*
CW K-G-300331 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563
CWK-G3001815 CWK-G-3001815 CWK-G-5000098 CWK-G-5000098 CWK-K-FBI-K-1 CWK-K-TBLK-1
0
"`k,.,
(laborat% duplicate) :.,
(laborator$?uplicate)
(taboratory d&~cate)
`:.
(laboratory
duplicate) `:, :.:, `.`...., :.,
C4 acid fnglml)
ND ND ND ND ND ND ND ND ND ND
limit of Detection (LOD) for the procedure? approximately `.,
Limit of Quantitation (LOQ) for the procedure'is, 36 ng/mL I.,
ND - Compound not detected
7.2 ng/mL
NQ - Compound detected at a level between the L$ NDeLODcNQcLOQ
and LOQ. Result is not quanttfiable.
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1.. `... `...
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ResearchDrive
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CWK-G-3003310
CWK-G-3000563 CWK-G-3001815 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-I
".:., `:. `.. `.., :., `.t.... `5, `:,
C4 acid (ng/mL)
ND ND ND ND ND ND
:.. Limit of Detection (LOD) for the procedure is approximately 7.2 ng/mL
Limit of Quantitation (LOCI) for the proceckre is 36 ng/mL
`..,
ND - Compound not detected
`\`..,
`:
NQ - Compound detected NDcLOD<NQ<LOQ
at a level between
the..,LOD and LOQ. :., `... `.., "
Result is not quantifiable.
Results are calculated according to the following criteria `...
If the sample and laboratory duplicate are greater than 6X. LOQ,
(RPD) is less than 20, the average value is reported. If theIRPD
value is reported.
,$G `...,,
and the relative percent difference is greater than 20, the higher
If the sample and laboratory duplicate are less than 5X LOQ, and the absolute difference is less than
LOQ, the average value is reported. tf the absolute difference i&greater than LOQ, the higher value is
reported.
`:, `:.
Drive PA76801, USA 1039 .I580 exygen.com
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Analytical f&&iv Chambers Works PWS Sampling 2106
DuPont Sample
CWK-G-3003310 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-Q-3001815 CW K-G-5000098 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-1
`-.,
Ide&fication :., `::.
(laborat$y duplicate) `:.
(laborato$aFplicate) `Q.
(laboratory d$#cate) `..,
(laboratory
dupl&$e) :., `... . . .
C5 acid (nglmL)
ND' ND ND ND ND ND ND ND ND ND
Limit of Detection (LOD) for the procedure'@ approximately :.*
Limit of Quantitation (LOQ) for the procedure'i! 1.4 ng/mL
0.28 ng/mL
ND - Compound not detected
NQ - Compound detected at a level between the L$ NDeLODcNQeLOQ
and LOQ. -\. :.,
:.,
Result is not quantifiable.
:814X2.1039 F: 814231.1580 exygenam
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Analytical
ResuI&.~harnbers `:, `...
Works PWS Sampling 2/06
DuPont Sample Ideniificalion `1
CW K-G-300331 0 CWK-G-3000563 CWK-G-3001815
CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-I
`;`:, "i,.. `... :.. `... `.., x. `... `.,
L.
C5 acid (nglmk)
ND ND ND ND ND ND
limit of Detection (LOD) for the proce&re is approximately 0.28 ng/mL
Limit of Quantiiation (LOQ) for the proce$Te is 1A nglml
`..*
`...
ND - Compound not detected
$*`:.
NQ - Compound detected ND<LOD<NQ<LOQ
at a level between
t6G.LOD and LOQ. i, :., `... `\
Result is not quantifiable.
Results are calculated according to the following crit&@ :..
If the sample and laboratory duplicate are greater than 2X LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If tf&,.RPD is greater than 20, the higher
value is reported.
:*`...,
If the sample and laboratory duplicate are less than 5X LOQ, `&d the absolute difference is less than
LOQ, the average value is reported. reported.
If the absolute
difference
i&greater `.., :.. `.., :.
than LOQ, the higher value is
:814A72.1039 E 814.231.1580
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RESEARCH
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DuPont Sampfe fde&icatfon `..,
CW K-G-30033 10 CWK-G-3003310 CW K-G-3000563 CWK-G-3000563
CWK-G-300? 815 CWK-G-3001815 CWK-G-5000098 CWK-G-5000098 CW K-K-FBLK-I CWK-K-TBLK-1
"`k. (laborat duplicate)
:..
(laboratory'$rplicate) `..
(laboratory dupjjcate) `k.
(laboratory
dupli&te) `.., `*., `.
C6 acid (nglf-)
89.7 96.7
ND ND 150 149 ND ND ND ND
Limit of Detection (LOD) for the procedure& approximately `..,
Limit of Quantitation (LOQ) for the procedure'k, ND - Compound not detected
79.1 rig/L :.
15.8 rig/L
NQ - Compound detected ND<LODcNQ<LOQ
at a level between
the L% and LOQ. `~.. `..,
`.., 1,
Result
is not quantifiable.
`.., `:, `,., .-., `.., `.., `i. :.. `. `... .+., `.., :., `L.
Drive PA 16801, USA
1039 .I580 exygenam
CH ":... Proven Rew 1%.
`-.. `%,
Analytical Result&.fXambers Works PWS Sampling 2/06
DuPont Sample Ident&ation :..
CW K-G-300331 0 CW K-G-3600563 CWK-G-3001815 CW K-G-5000098
CWK-K-FBLK-1 CWK-K-TBLK-1
""' .`....,`... `... .... `.., X. `.. :,, `:,
C6 acid @g/L)
93.2 ND 150 ND ND ND
limit of Detection (LOD) for the procidure is approximately "
Limit of Quantitation (LOQ) for the procedure is 79.1 rig/L
`:,
`*.,
ND - Compound not detected
`..,
"
15.8 rig/L
NQ - Compound detected ND<LOD<NQ<LOQ
at a level between
tl&LOD and LOQ. 1, :.. `.., `:.
Result
is not quantifiable.
Results are calculated according to the following cr&a
If the sample and laboratory duplicate are greater than 5X LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. value is reported.
If the., RPD is greater than 20, the higher `.., `x, :.
If the sample and laboratory duplicate are less than 5X LOQ, And the absolute difference is less than
LOQ, the average value is repotted. reported.
If the absolute difference i&.$!reater than LOQ, the higher value is :.. :.,
: 814.272.1039
F: 814237.4580
exygen.com
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Analytical
Rest.& Chambers `9.. `i,
Works
PWS Sampling 2/06
DuPont Sample Ide&ication `...
CW K-G-30033 10
"`-..
CWK-G-3003310 CWK-G-3000563
(laboratory duplicate) :.,
CWK-G-3000563 CWK-G-3001815
(laboratory'duplicate) !,
CWK-G-3001815 CWK-G-5000098
(laboratory du$cate) :..
CWK-G-5000098 CWK-K-FBLK-1
(laboratory duplicate) `:,
CWK-K-TBLK-`!
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C7 acid (nglL)
NQ NQ ND ND 86.9 73.8 ND ND ND ND
Limit of Detection (LOD) for the procedur2i.s approximately t.
Limit of Quantitation (LOQ) for the procedure'&, 61.2 rig/L
`..,
l.
ND - Compound not detected
"
12.2 rig/L
NQ - Compound detected NDcLOD<NQ<LOQ
at a level between
the LCD and LOQ. " `I `.`..,, `:, :.,`:, :.. `..
Resuft is not quantifiable.
Analytical
Resu&.,~hambers `... `*.
Works PWS Sampling 2fO6
DuPont Sample Iden&ation `,
CW K-G-30033 10 CWK-G-3000563 CW.K-G-3001815 CWK-G-5000098 CWK-K-PBLK-1 CWK-K-TBLK-I
"`.,,.., ":,, `C, .*.. `.., :.. `..* :.. `.)
C7 acid (ngiL)
NQ ND 80.4 ND NR ND
Limit of Detection (LOD) for the procedure is approximately :,
Limit of Quantitation (LOQ) for the procedure is 61.2 rig/L `..,
ND - Compound not detected
12.2 rig/L
NQ - Compound detected at a level between tJ&.`..L.O, D and LOQ. Result is not quantifiable.
`i,
ND<LOD<NQ<LOQ
`:. :.,
Results are calculated according to the following crJt&a `..
If the sample and laboratory duplicate are greater than 2X LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If th&.RPD is greater than 20, the higher
value is reported.
:.. :.,
`..
If the sample and laboratory dupJicateare lessthan 5X LOQ, And the absolute difference .isless than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
:., `..,
`:,
:... Proven Results. :.,
Analytical Res& Chambers Works PWS Sampling 336
DuPont Sample
CWK-G-300331 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-G-3001815 CW K-G-5000098 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-1
Ide&fication :
`.,
0
"...,
(laboratby duplicate)
`::
(laboratory'$plicate) `a.,
(laboratory (laboratory
du$cata) ";:
dupli&+e) :.. " `:,
APFO @g/L)
124 131 ND ND 187 169 ND ND ND NQ
PFOA @g/L)
119 126 ND ND 180 162 ND ND ND NQ
Limit of Detection (LOD) for the procedurij? approximately 2.3 rig/L
Limit of Quantitation (LOQ) for the procedure'@, 12 rig/L
`.. ND - Compound oot defected
NQ - Compound detected at a level between the L* NDcLODcNQeLOQ
and LOQ. `. `...
\., .
Result is not quantifiable.
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Result@hambers `... `*., :..
DuPont Sample Iciend@dion `...
Works.PWS
CW K-G-300331 0 CWK-G-3000563 CWK-G-3OO'l815 CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-1
I';-. `.., . . . `.., `\`I `.., .*.. . . . `... `:, `i.
Sampling 2106
APFO (ngk)
128 ND 178 ND ND NQ
Limit of Detection (LOD) for the proce'&Fe is approximately `...
Limit of Quantiiation (LOQ) for the procec&.re is 12 rig/L
2.3 rig/L
PFOA (rig/L)
123 ND 171 ND ND NQ
ND - Compound not detected
NQ - Compound detected ND<LODcNQ<LOQ
at a level between
tk., LOD and LOQ. :., `.., `-..
`,
Result is not quantifiable.
Results are calculated according to the following crit&a .Y
If the sample and laboratory duplicate are greater than k LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If th&,.RPD is greater than 20, the higher
value is reported.
l,
If the sample and laborakry duplicate are Iess than 5X LOQ, i?!d the absolute difference is less than
LOQ, the average value is reported. reported.
if the absolute difference id,greater than LOQ, the higher value is `:, `:.
College,PA16801, USA
: 814272.1039 F. 814.231.1580
exygervmm
RESEARCH
`:, `-..
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`...
Analytical Res$i Chambers Works PWS Sampling 2106
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DuPont Sample Idehyfication
`..,
CWK-G-300331 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-G-3001815
CWK-G-5000098 CWK-G-5000098 CWK-K-FSLK-1 CWK-K-TBLK-1
0
`::.
(laboratory duplicate) :.,
(laborato$duplicate) `...
(laboratory duplicate) `:,
(laboratory
dupli&te) :.. `., `:,
C9 acid (nglL)
NQ NQ ND ND NQ NQ ND ND ND ND
Limit of Detection (LOD) for the procedureI approximately 13.6 rig/L
Limit of Quantiition (LOQ) for the procedure'is ND - Compound not detected
67.8 rig/L `i,
.... `..
NQ - Compound detected NDcLODcNQcLOQ
at a level between
the L&D and LOQ. `... `k,
Result
is not quantifiable.
: 814.272.1039 exygen.com
Analytical
Result&Chambers `.., `-., :..
Works PWS Sampling UO6
DuPont Sample Ident@cation `...
CWK-G-3003310 CWK-G-3000563 CWK-G-3001815 CW K-G-5000098
CWK-K-FBLK-1 CWK-K-TBLK-1
"j `.., :.* :.. `.., L, `-., .*.. `.. .... `..
Limit of Detection (LOD) for the procedure is approximately j3.6
CQ acid (rig/L)
NQ ND NQ ND ND ND
rig/L
Limit of Quantitation (LOCI) for the procedure is 67.8 ngR :.
ND - Compound not detected
NQ - Compound detected ND<LODcNQ<LOQ
at a level between
tt&.LOD and LOQ. >.
`a..
`L.
Result is not quantifiable.
Results are calculated according to the following criteria
If the sample and laboratory duplicate are greater than & LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If the..,RPD is greater than 20, the higher
value is reported.
`...
If the sample and laboratory duplicate are less than 5X LOQ, i!d the absolute difference is less than
LOQ, the average value is reported. If the absoiute difference i&.$m?ater than LOQ, the higher value is
reported.
`..,
College, PA 16801, USA F: 814.231.7580
exygen.com
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`.., :.. `:,
Anafytical Res& Chambers Works PWS Sampling 2106 `.., -.,
DuPont Sample Idehf~cation --.
CW K-G-300331 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563
CWK-G-3001815 CWK-G-3001815 CWK-G-5000008 CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-1
0
\`:.
(laboratby duplicate) :.,
(laboratory$,uplicate) `:,
(laboratory d&jicate) `..,
(laboratory dupli&te) `... `..,
Cl 0 acid (nglL)
ND ND ND ND ND ND ND ND ND ND
Limit of Detection (LOD) for the procedure'& approximately 20.6 rig/L
Limit of Quantitation (LOQ) for the procedure is, 103 rig/L :.,
ND - Compound nr% detected
NQ - Compound detected ND<LOD<NQeLOQ
at a level between
the L&D and LOQ. `,., `... :.,
Result is not quantifiable.
058 l&se& Cokge,
&ii PA 76801, USA
exygenxom
E CH
Analytical Result&hambers
Works PWS Sampling 306
DuPont Sample Iden&iwtion `..,
CWK-G-3003310 CWK-G-3000563 CW K-G-3001 815 CWK-G-5000098 CWK-K-FBLK-I CW K-K-TBLK-I
Limit of Detection
(LOD)
!,, `.`,.., `..
`:.. `.., `...
`...
" .... `..,
for the procedure
is approximately
Cl0 acid (rig/L)
ND ND ND ND ND ND
20.6 rig/L
Limit of Quantitation (LOQ) for the proced'wre is 103 rig/L
" \..
ND - Compound not detected
`*.
NQ - Compound detected ND<LOD<NQcLOQ
at a level between
the.,.LQD and LQQ. `,. `.., `\:..
Result
is not quantifiable.
Results are calculated according to the folfowing c&&a
`-.,
If the sample and laboratory duplicate are greater than <$lOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If the,,.RPD is greater than 20, the higher
value is reported.
`;.
`:,
If the sample and laboratory duplicate are less than 5X LOQ, 2nd the absolute difference is less than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
`..,
058 Re.search Drii College, PA 16801, USA
exygen.com
`..,
:., `... `...
Analytical
Resuh? Chambers `," `.,
Works
PWS Sampling 2lO6
DuPont Sample Ider$fication
Cl1
CWK-G-3003310 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-G-3001815 CW K-G-5000098 CWK-G-5000098 CWK-K-FBLK-I C@,`K-K-TBLK-1
`..,
`:, (laborat@ duplicate)
`.:. (laboratory$uplicate)
(laboratory d&!kate) `:.
(laboratory.dupli&~e) I., `... `:, :.
`:. :..
Limit of Detection (LOD) for the procedure'? approximately 12.2 rig/L `...
Limit of Quantitation (LOQ) for the procedure ND - Compound not detected
`Is 60.8 rig/L `.., `:, :., :..
acid (ngll)
ND ND ND ND ND ND ND ND ND ND
NQ - Compound detected ND<LODcNQcLOQ
at a level between
the L&F and LOQ. %., `: :, :., :., `... `:, `.:..,. :..
Result is not quantifiable.
`i, `.., :..
`..., .:,
exygen.com
"..
Analytical F4txx~l~~.,~hambers Works PWS Sampling 2/06 `... `... `.,.
DuPont Sample Ident@ation
CW K-G-30033 I 0 CWK-G-3000593 CWK-G-3001815 CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-1
"`.:., `.., `i, :., `.., `::., " `:, :., `...
Cl1 acid (nglL)
ND ND ND ND ND ND
Limit of Detection (LOD) for the proce&re is approximately 12.2 rig/L
Limit of Quantitation (LOQ) for the procedqre is 60.8 w/L
ND - Compound not deteoted
NQ - Compound detected at a level between ti&,,LOD and LOQ. Result is not quantitiable.
`,..
NDcLODcNQcLOQ
`:. `l.
Results are calculated according to the following crit&si.
If the sample and laboratory duplicate are greater than 8X LOQ, and the relative percent difference
(RPD) is lessthan 20, the average value is reported. If the.,.RPDis greater than 20, the high&
value is reported.
i.`...
If the sample and laboratory duplicate are less than 5X LOQ, 2nd the absolute difference is less than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
I.
exygen.com
E
CH
:..,. Proven
:., :.`,...
:.`,..,
Results.
Analytical Red.? Chambers,Works `..,
PWS Sampling 2/06
DuPont Sampie Ide+ficatiOn
'
CWK-G-300331 0
`j
CWK-G-3003310 (laboratby duplicate)
CW K-G-3000563
:...
CWK-G-3000563 CWK-G-3001815
(laboratoty'+plicate) `,`.
CWK-G-3001815 CWK-G-5000098
(laboratory du&icate) `.,
CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-I
(laboratory dupli&?e) `.., :..
Cl2 acid (rig/L)
ND ND ND ND ND ND ND ND ND ND
Limit of Detection (LOD) for the procedur:.$ approximately 13.2 rig/L
. .
-,
Limit of Quantitation (LOQ) for the procedu$iq 66.2 ngL
;..
ND - Compound not detected
:.,
`%.
NQ - Compound detected NDcLODcNQcLOQ
at a Ieve between
the L&p and LOQ. `... `. +... `.., `::,.
Result
is not quantifiable.
30
Drive PA 16801, USA
1039 .I580 exygerxom
E CH
`:* `k.
`... -.. `,.. :
Analyticaf Resuf&..Chambers
Works PWS Sampling 2106
DuPont Sample Iden$cation :..
CWK-G-30033 1 0 CWK-G-3000563 CWK-G-3001815 CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-1
`::., `... :.. `.., `.I..,, `.., :.. ;.. I.,
`:,
Limit of Detection (LOD) for the proce$re is approximately i,
Limit of Quantitation (LOQ) for the proce&.ra is 66.2 rig/L
Cl2 acid (rig/L)
ND ND ND ND ND ND
13.2 rig/L
ND - Compound not detected
:.,
`...
`..,
NQ - Compound detected ND<LODcNQ<LOQ
at .a level between
ti$,,LOD and LOQ. `.,. `., `:, `:,
Result is not quantifiable,
Results are calculated according to the foliowing crit&?
`.
`...
If the sample and laboratoty duplicate are gkater than !%SLOQ, and the relative percent difference
(RPD) is kss than 20, the average value is reported. If t&,.RPD is greater than 20, the higher
value is reported.
%.,
`9..
If the sample and laboratory duplicate are less than 5X LOQ, &d the absolute difference is less than
LOCI, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
`..,
E CH
Analytical Res& Chambers Works PWS Sampling 2106
DuPont Sample
CW K-G-300331 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-G-3001815 CWK-G-5000098 CWK-G-5000098 CWK-K-FBI-K-1 CWK-K-TBLK-1
Id@fication ....
0
".:.
(laborai* duplicate)
`::
(laboratory'dupiicate) `...
(laboratory dkpJ!cate) :..
(Laboratory dupli$Je) `...
PFHxS @g/L)
ND ND ND ND ND ND ND ND ND ND
Limit of Detection (LOD) for the procedurf&~ approximately `*.
Limit of Quantitation (LOQ) for the procedur&T 8.5 rig/L
1.7 rig/L
ND - Compound not detected NQ - Compound detected at a level between ND<LODcNQ<LOQ
`...
:., `.. the L&I and LOQ.
.... :., :.,
:.,
Result
is not quantifiable.
58 Research Drive
College, PA 16801, USA
:814.272.1039
exygen.com
E
CH
`...,, Proven Results. :.. `.., `,., `..,
:..
Analytical
Resuf&.$3wmbers `:. :., Y,
Works PWS Sampfing 2/06
DuPont Sample fdenth$xtion t,
CW K-G-30033 IO CWK-G-3000563 CWK-G-30018-l5 CWK-G-5000098 CWK-K-FBLK-`l CWK-K-TBLK-1
",...,,
.": `.., `.., `., `:, `.., `..,
PFHxS (rig/L)
ND ND ND ND ND ND
Limit of Detection (LOD) for the procedure is approximately 1.7 rig/L :.,
Limit of Quantitation (LOQ) for the procedqre is 8.5 rig/L
`:`,...
ND - Compound not detected
`j`\
NQ - Compound detected at a level between t&,LOD and LOQ. Result is not quantifiable,
`L,
:.,
ND<LOD<NQ<LOQ
`..,
Results are calculated according to the following wit&@
If the sample and laboratory duplicate are greater than 2%LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If the,,RPD is greater than 20, the higher
value is reported.
`.:...
:.
If the sample and laboratory duplicate are less than 5X LOQ, 2nd the absolute difference is less than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
:.,
F: 8'14.231.1580 exygen.com
E CH
Analytical
Res&~
Chambers `.I.
`..,
Works
PWS Sampling 2/06
DuPont Sample Ide&fication !,
CW K-G-30033 10 CWK-G-3003310 CW K-G-3009563 CWK-G-3000563 CWK-G30018'l5 CWK-G-3001815
CWK-G-5000098 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-I
`~"z. (IaboratByy duplicate)
`.., (laboratory'~~plicate)
(laboratory (laboratory
d&jkate) `...
duplic$!e) ".`... `, :.
PFOS @g/L)
ND ND ND ND ND ND ND ND ND ND
Limit of Detection (LOD) for the procedure'.@ approximately "
Limit of Quantitation (LOQ) for the procedure'@ 35.2 rig/L `:,
ND - Compound not detected
7.0 rig/L
NQ - Compound detected at a level between the Lb? and LOQ. Result is not quantifiable.
NDcLOD<NQcLOQ
i3t?wl1, USA
1039 .1580 exygekcom
E
RESEARCH
Analytical
>., Resul&.,Chambera
. . . `j
Works PWS Sampling 2106
DuPont Sample Iden&i.~cation
-...
CWK-G-30033 10 CWK-G-3000563 CWK-G-3001815
CWK-G-5000098 CWK-K-FBLK-I CWK-K-TBLK-1
",...., :.. `:, `.. :.., `1 `.., :.,
PFOS (nglL)
ND ND NP ND ND ND
Limit of Detection (LOD) for the procedure is approximately 7.0 rig/L
Limit of Quantitation (LOQ) for the proce&re is 35.2 rig/l.
`...
:..
ND - Compound not detected
`..,
`.,
NQ - Compound detected at a lever between
ND<:LOD<NQ<LOQ
the.,LOD and LOQ. `... `.., `...
Result is not quantifiable.
Results are calculated according to the following criteria
If the sample and laboratory duplicate are greater than !& LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. If th&,+RPD is greater than 20, the higher
value is reported.
:.,
If the sample and laboratory duplicate are less than 5X LOQ, 2nd the absolute difference is less than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
..a.
`,.,
`.,
exygen.com
CH
Analytical Res& Chambers Works PWS Sampling 2/06 ".. " `L,
DuPont Sampie Ide@icatlon
CW K-G-30033 10 CWK-G-3003310 CWK-G-3000563 CWK-G-3000563 CWK-G-3001815 CWK-G-3001815 CW K-G-5000098 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-1
`:.
`:.. (labor&cry duplicate)
i,
(laborato~`$rplicate) `..*
(laboratory d&]icate) `i*
(laboratory
duplic?te) , `; "., .*.. :.,
FOSA @g/L)
ND ND ND ND ND ND ND ND ND N?
Limit of Detection (LQD) for the procedure'q approximately :.*
Limit of Quantitation (LOQ) for the procedure'is 1.7 ngR :..
ND - Compound not detected
0.3 rig/L
NQ - Compound detected NDcLOd<NQ<LOQ
at a level between
the LbD and LOQ. :., :., `,., .:
Result
is not quantifiable.
36
Analytical
`.., :.,
`:. `:, " :.. `.., :,
Resul&.,~hambers :..
`:
Works PWS Sampling 2/06
DuPont Sample iden&cation `..,
CW K-G-300331 0
CW K-G-3000563 CWK-G-3001815 CWK-G-5000098 CWK-K-FBLK-1 CWK-K-TBLK-1
`!.,
"...,,, . . . `... .,`..,., `.., `:
FOSA (nglL)
ND ND ND ND ND ND
Limit of Detection (LOD) for the procedyre is approximately 0.3 rig/L
Limit of Quantitation (LOQ) for the procedure is 1.7 rig/L
ND - Compound not detected
NQ - Compound detected NDcLODcNQcLOQ
at a level between
tl-&,.lOD `., `:`,..,
and LOQ.
Result is not quantifiable.
Results are calculated according to the foflowing crit&j,a `:
If the sample and laboratory duplicate are greater than 2X LOQ, and the relative percent difference
(RPD) is less than 20, the average value is reported. value is reported.
If the.. RPD is greater than 20, the higher `X, :., `...
If the sample and laboratory duplicate are less than 5X LOQ, and the absolute difference is less than
LOQ, the average value is reported. If the absolute difference i&greater than LOQ, the higher value is
reported.
`:,
ege, PA 16801, USA E 814231.7580
exygen.com
Address: City State, ZIP: Attention:
Fax #: Email: Study/lob #: Signature/Date: Printed Name:
Sample Submittal
Please fax thisibrm before sending samples.
Please send samples to Exygen's shipping and receiving address:
3048 Research Drive, State College, PA 16801
T.*C)1A?`I?In2n,Lo,n
373If-tlO
Turnaround time (TAT) requirements:
Results Due Date:
Preliminary Results Format: 0 Verbal 0 Email 0 Fax
Report Due Date: I
1 Storage conditions
0 Room temperature 0 Refrigerator 0 Freezer 0 Ultra-Low freezer 0 Desiccated p Lighting required
Stability ("C/%RH):
h# w&&w
Stability time period:
Safety information
Special handling: 0 MSDS attached 0 Controlled substance:
Cl HAZARDS:
Pleasefiil in the diamond HMIS/NFPA (O-4) a appropriate
Notes:
Administrative Form
DuPont
AQUEOUS,SAMPLES
PERFLUORINATED ORGANIC% ANALYSIS
AXYS METHOD: NILA-040
Contract: 4380 Data Package Identification: WG18696
Analysis WGl85& and WGl8650
Prepared for: DuPont Corporate Remediation
4417 Lancaster Pike Bldg Willmingon DE USA 19805
Group 19
Prepared by: AXYS Analytical Services Ltd. P.b, Box 2219,2045 Mills Rd West Sidney, Britiish Columbia V8L 358
CANADA
Contact: Todd Fisher Project Manager
21 March 2006
CLIENT ID AXYS ID WORKGROUP Sample Size UNITS PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA
CWK-G-3003310 L8701-I (A) WGI 8544 0.501 L
rig/L 16.3 40.3 42.2 21.5 36.7 9.74 1.31 1.19 c 1.13 c 6.23 2.86 6.15 < 0.918
CWK-G-3000563 La701 -2 WG18544 0.503 L
W < 1.20 < 2.20 < 1.29 < 1.06 < 0.963 < 1.35 < 1.07 c 0.947 < 1.13 < 2.40 < 2.01 < 2.00 < 0.940
CWK-G-3001815 L8701-3 WG18544 0.500 L
ngR 18.6 66.3 75.5 38.9 57 12.7 4.06 < 0.953 c 1.14 c 2.41 3.32 3.21 c 0.921
CWK-G-5000098 CWK-K-TBLK-1
l-8701 -4
La701 -6
WG18544
WG16544
0.500 L
0.500 L
n@-
nd-
cl.21
:. <I.21
c 5.95
< 0.944
c 0.968
co.968
< 1.06
< 1.06
< 1.01
c 0.968
c 1.36
c 1.36
< 1.07
< 1.07
i 0.952
< 0.952
c 1.14
c 1.14
< 2.41
c 2.41
< 2.02
< 2.02
< 2.01
< 2.01
< 0.920
< 0.920
Lab Blank WG18544-101
WGI 8544 0.500 L
ngR < 1.21 < 0.944 < 0.968
< 1.06 c 0.968
Cl.36 c 1.07 < 0.952
c 1.14 c 2.41
c 2.02 < 2.01 c 0.920
Spiked Matrix WG18544-102
WG18544
% Recov 94.4 97 96.4 107 105 108 106 99.2 94.9 102 84.4 68.7 85.9
CWK-G-3003310 (Duplicate)
WG18544-103 (DUP L8701-1) WG18544 0.249 L
n9lL 21.1 41.3
43.4 26
38.9 9.12 c 2.15
< 1.91 c 2.28
< 8.36 < 4.06 6.14
< 1.85
See below for definitions of possible flags and labels in the database (sheet tab `GenericEDD')
<
=
less than the detection limit
number following this symbol represents the detection limit
For homologue totals sums, please see the individual congener data for the detection limit.
E
=
exceeds calibrated linear range, see dilution data
CLIENT ID AXYS ID
WORKGROUP Sample Size UNITS PFBA
PFPeA PFHxA PFHpA PFOA PFNA
PFDA PFUnA PFDoA PFBS PFHxS
PFOS PFOSA
CWK-K-FBLK-1
L8701-5 WG18650
0.502 L
rig/L < 1.20 c 0.941 c 0.965 < 1.06
c 0.965 c 1.36 < 1.07 c 0.949 < 1.13 c 2.40
c 2.02 c 2.00 c 0.917
Lab Blank WG18850-101
WGl8650 0.420 L
lIg/L c 2.31 cl.12 c 1.15 < 1.27
c 1.15 c 2.00 c 1.28 c 1.13 Cl.35 c 2.87
c 2.47 c 2.39 c 1.10
Spiked Matrix WG18650-102
WG18650
% Recov 88.7 90.7 96.8 92.9 102 111 73.8 80.1 101 109 103 103 91.2
See below for definitions of possible flags and labels in the database (sheet tab `GenericEDD')
C
=
E
=
less than the detection limit number following this symbol represents the detection limit For homologue totals sums, please see the individual congener data for the detection limit.
exceeds calibrated linear range, see dilution data
Post office Box 2219,2045 Mills Road WestTEL: (250) 6554800 Sidney, British Columbia. Canada V8L 358 FAX: (250) 655-5811
CHAIN OF CUS-fODY
'
AXYS CLIENT #: