Document B8xyx4v4bMLOEZZoVVyYBQmyJ

BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctnaesulfonate which may also be referredtoas PFOS, FC-95, oras a component ofFC-203FC. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-203FC, identifiebdy the laboratoryas Rl 335. Currentinformation indicatesitisa mixtureof0.85% PFOS, 17% diethyleneglycolbutylether, 74.46% water,2.34% acrylifcoamer, 1.7% alkylethersulfate2,.55% sodium octylsulfate0,.12% sodium nitrit0e.,09% sodium molybdate,0.85% tdethanolamine,and 0.04% tolyltriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectdegradation of the fluorochemkal component of the testsample. METHOD: Method: OECD Guideline301 D Type: Aerobic GLP: No Year completed: 1997 Contacttime: 28 days Inoculum: Polyseed NX, Lot D257, Polybac Corporation,Bethelem, PA. RESULTS Chemical Oxygen Demand (COD): BiochemicalOxygen Demand (CBOD): 5-Day: 1O-Day: 20-Day: 28-Day: 447,000 mg/kg Replicate- 1 <95,000 mg/kg 199,500 mg/kg 294,900 mg/kg 301,500 mg/kg eplicate2 <95,000 mg/kg 224,800 mg/kg 274,800 mg/kg 290,000 mg/kg Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS@ No conclusionsgiven. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2. This study meets the criterifaorquality testing.However, the sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationofthe amount of fluorochemicalproportion insolution. REFERENCES This testwas conducted by ASci Corporation,Duluth EnvironmentalTesting Division,Duluth,MN atthe requestofthe 3M Company, Lab Request number R1335,1997. OTHER Last changed: 6/26/00 ASci Corporation/ASel-Duluth EnvironmentalTestingDivision Subntittetdo 3M Company - 9/97 ASel-DETD Study ID #5010-069 R1335 BOD REPORT FOR 3M COMPANY Submitted to 3M EnvironmentalTechnology and Safety Services 935 Bush Avenue Building2-3E-09 PO Box 33331 St. Paul,MN 55133-3331 Prepared by ASci Corporafion/AScl-Duluth Environmental Testing Division 4444 AirparkBoulevard Duluth,Minnesota 55811 Sponsor: 3M Company Sponsor Study [D# R1335 SEPTEMBER 1997 AScl C.rpomtion/ASel-Duluth Environmental TestingDivision Submitted to 3M Company - 9/97 ASel-DETD Study ID #5010-M COD = 447,000 mg 02 1 kg chemical. Datesof BOD test:8/22/97- 9/19/97. Water bathtemperaturerangeduringthetest:19.6- 22.0*C (controllesrhortedout on 9/7/97 ovemight,temperatureoutof rangeforabout 16 hours.) Lot number of PolyseedNX: D257. TABLE 1. BOD DilutionInformationforR1335 SUVIC ED (mg/L) 9 13 17 21 StockSolution Concentration(MS/L) 984 984 994 984 Sample Volume (mi) 2.7 4.0 5.2 6.4 Sample Concentration (ms/L) 9.9 13.1 17.1 21.0 TABLE 2. SOD Blank CalculationsforR 1335 Sample ID Daayy m Blank 5A 5 Blank Blank Blank 10 L21' 20 29 FIRST REPLICATE -initiaDl.O. FinalD.O. 9.94 9.94 9.94 8.94 9.79 9.60 9.61 r 8.64 D.O. Drop 0.15 0.34 0.33 0.30 SECOND REPLICATE InitiaDl.O. FinalD.O. D.O. Drop 8.% 9.96 8.96 8.96 9.79 9.42 9,54 8.18 0.18-@ 0.54 0.42 0.79-- Sponsor: 3M Company Sponsor Study InD# R1335 ASci Corporation/AScI-Duluth EnvironmentalTesting Division Submitted to 3M Company - 9t97 AScl-DETD Study ID #5010-068 TABLE 3. BOD Seed CorrectioFnactorCalculationfsorR1335 Seed Cone. Day mvsottle FIRST REPLICATE Initial D.O. Final D.O. D.O. Drop 10 5 8.92 7.10 1.92 1'0o 10 m 10 8.92 2O21R00sy8.92 8.92 23 3.92 6.20 4.42 4.62 - 2.72 4.50-4.30 3CFa 0.36 0.54 0.90 0.96 SECOND REPLICATE Initial D.O. Final D.O. D.@O..p D=Drop 8.95 8.95 8.95 1 9.95 6.81 5.64 4.64 3.83 2.14 3.31 4.31 5.12 SCF (D.O. drop * mi seed in test)/ ml seed in seed blank. SCF 0S.C44F33 03@6..16866 1.02 0. Avi:mgc SCF 0.40 0.60 0.88 TABLE 4. BOD Referencea'nd Tox BlanksCalculatiofnosrR1335 Conc. Day SCFF0 MS/L wounim@ roM @0Lnc. 5 400ST.C40 L21.0 5 _1_0.40 FIRST REPLICATE I9nn.i_i0t0t0_iiaall Final D.O. D.O. D.O. Drop Coffected BOD Drop mW 9.00 9.05 5.00 4.00 3.60 ISO 4.24 1 4.81 1 4.41 IN SECOND REPLICATE Initial Final D.O. D.O. D.O. Drop Coffected 'EbD Drop 4g/L 9.00 9.00 4.90 3.62 4.20 5.38 3.80 4.99 10 249 I Reference = 150 mg glucose and 150 mg glutamic acid in I literBOD dilution. dilutionwater, tested at 2% b BOD mg/L (correctedD.O. drop) 300 mi BOD volume 6 ml reference. -2 TABLE 5. SOD CalculationfsorR1335 ASel Corporation/ASci-Duluth Environmental TestingDivision Submitted to 3M Company - 9/97 ASel-DETD Study ID #5010-06s Conc. mg/L Day SCF FIRST REPLICATE lwtki Final D.O. @D D.O. D.O. Drop Corrected Drop SOD mglke 9.9 5- 0.40 9.00 9.11 0.89 -0.49 13.1 17.1 21.0 5 0.40 9.01 5 0.40 8.97 5 0.40 9.00 8.0-4 7.30 7.42 0.97 1.67 1.58 0.57 1.27 1.18 < 95,000 Mean < 95,000 wtid D.O. 9.03 9.03 B." 8.97 SECOND REPLICATE Final D.O. 7.84 D.O. Drop p 1.19 Corrected Drop 0.79 BOD mg/kg 7.92 7.61 7.30 1.11 1.38 1.67 0.71 0.98 1.27 < 95, Mean 95,000 8.9 113.10 13.1 17.1 17 2F21.0 10 0.60 9.00 10 0.60 9.01 10 0.60 8.97 10 0.60 9.00 7.34 7.16 6.61 4.21 1.66 1.85 2.36 4.79 1.06 1.25 1.76 4.19 1-199,500 Mean 1",500 9.03 9.03 8.99 8.97 7.28 7.04 6.94 3.65 1.75 1.99 2.05 5.32 1.15 1.39 1.45 -4.72 -224,800 Mean '))4ROO 8.9 20 0.88 9.00 5.30 3.70 13.1 20 0.89 9.01 4.62 4.39 17.1 20 0.98 8.97 2.96 6.01 21.0 20 0.89 9.00 <0.50-1 2,82 3.51 5.13 316,900 267,900 300,000 9.03 9.03 9.99 Mean 294,900 5.51 4.31 4.14 .50 3.52 4.72 4.85 - 2.64 3.94 3.97 - 296,600 293,100 .232,200 -- Mean 274,000 8.9 28 13.1 28 17.1 28 21.0 1 23 0.94 9.00 5.18 0.94 9.01 4.34 0.94t 8989._.*090970'7 2.66 0.94 9. 1 2.10 -3.82 4.67 6.31 6.90 2.89 232,600 3.73 294,700 5.37 5.96 314,000 1 293 Mean 30 9.03 9.03 a." .97 5.80 4.41 2.80 1.42 3.23 4.62 6.19 7.55 2.29 3.68 -257,300 :Z$0,900 5.25 3@07,000 6.61 314,800 Mca!L290,ODO BOD coffwted drop x 1,000,000 testconcentration. .Qmitted data RQints@ D.O. drop Corrected for SCF Final D.O. <0.50 mg/L. too low (<2.0). Sponsor: 3M Company Sponsor Study tD# R1335 -3-