Document B8vbDmDp5Ovon54dDqVZBq7d4
Report EO1-1256
PFOS: A Reproductizn Study with the Mallard
AR226-183'
ANALYTSICtALuPdHyASETiREtPlOeRT FOR PFOS: A REPRODUCTION STUDY WITH THE MALLARD
FIFDRaAtSaubdRiveisqioun iE,rSeecmtieonn7t1.4 OECD Guideline 206 LisaAAu. tSthevoernson
Analytical FPebhruaarsye19,I2n0i0t2iation Date Analytical PhaAstSeignCiongmpletion Date
PerfoMremnionmegnaLaabboorrtatory [rBourkisngv235n.00 Spat tn 5108
3M EnPvirroonjmeenctatl LIabdoeranttoriyfSitcuad#tyiEo0n1-1256 Widife International Ltd: 454-109
Total Numberof Pages 26
3M Environmental Laboratory
Page 1
GLO62
Report E01-1256
fo
PFOS: A Reproductizn Study with the Mallard
2708: AaStr y inat ts
GLP COMPLIANCE STATEMENT
StudyTi: Analytica Phase Report or Stuy lgenifcaton Number: E01-1250
PFOS:
A
Reproduction
Study
wih
the
Malard
T(hGiosodanLaalbyotricaatlorpyhaPrsaectwiacsa c(oGnLdPu)cStteadndinacrosmpwiiahntcheeweixicheTptoiiocnsSusblsetdanbceelsowCarrol Act (TSCA)
Excoptions 0 GLP compiance:
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PROS: AReproduction Study it teMilind A
SSQUynALTe IhTeY,Am SASUnR-- AtNCEPraSsTAERTeErMpErNoTPES: A Ratton Sty wi Mar ISoiaevaei pnhssswaansuettya34EEsmecrorrttlLLaabrriye OGunlutyAsstaUt
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ReportEOI-1256 ReponEdt 258
PROS: AReproStduduywicthttheiMaollanrd PFOS: AReproSdyuwcththaiWoasns
TABLE OF CONTENTS GLP Compliance Statement. Quality AssuranceStatement LitOfTab...
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conmmmnimnll SY commands
ReferenceSubstances. com----"
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8
Specimen ReceiptandSIOMDG
server
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MethodSummary..........
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PIODATBONY MONO ccs
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`SampleReceipt,Propaarndaton ANaISi.....
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Analytical Method........ o--
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Analytical Resuls.....
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StatoefCmonceluniotn......
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Statistical Methods.
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StatementofData Quaiy. sm----
sommmnnill
References...
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ListofAtachments
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`AAntaalycshismeofnAFt:uoErxotcrhacetmiiocnaalnCdomAnpaolyutnicdaslfMreotmhBoido,agEiTcS-a$l:2M31a.I1r,ic"eSsolid Phase Extraction and. 1
AachenB:DataSUMMary Tables...
wom
tachmenCt: Sample Chromatograms, Celbratin Information, snd Instrument Information... 55
AttDa:SacmplhePremparaeionnt SNRs...
--1
tachmenEt: StudyProtocol, ProtocolAmendments, and Deviations .............
8
AttachmenFt:Example CAcUIONS..........
smm--
Eriol ons
3MEnvironmental Laboratory
pacescr216
Paged
CNLOBS5
Report EO1-1256. Ropon enn 258
PROS: A Reproduction Study with the Mallard PFOS: AReproSdyuwcihtaitoanrs
List oF TABLES
`Table 1. SummaryTable,C4;SO5 Concentratioin Sora and Liver
nT
"Table2. SummaryTable, Cf S05 Concentratioin Sera and Liver with Outliers Removed......8
Table 3. Reference Substances...
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5
Tabl4e. SPE Extraction Summary
sham--
cd
Table 5 Sample Analysis...
SE
--
`Table . Serum QCSummary Tabl.
r----
13
Table7. Serum Matix Spike Summary Table
cunmnen----
3
Table8.Liver QC Summary Table.
we
mmm
Tablo 9. Liver Matrix Spike SUMMOry Table...
stl
"Table 10. CFSO5 Data Summary, Femalo MallardSora(Adult and OfSprin)......... 14
Table 1. CFSOs Data Summary,MaleMalSleraa(rAudlt and OOH)... 16.
Table 12. CFS05DatoSummary,FemaleMalardLiver (AdultandOHSPINg).....cc.cvc 18.
Table 13. CFS05 Data Summary, Male Mallard Liver (Adult and Offspring)
--
Table 14. Summry Table, CyF,SO Concentiatiion Seraand Liver.
nin
`Table 15. Summary Table,CyFSOx Concentration inSeraandLiverwithOutlers Removed ....23
3M EnvironmeMnEtnamleLsanbmaonrsattoLrayvoro
Page sorate Pages
GN1LOS6
Report EOL-1256 RoponEor 125
PFOS: AReproduction Study with the Mallard POS: ARproScytweihtshMoatin
_--
STUDY INFORMATION
`Study Dioctor
Sean P. `Wide
Gallagher Intemational,
Lid
E8a5s9t8onC,oMmamreyrlcanedDr2i1v6e01
`Sponsor 33MM EEnnvviirroonnmmeennttaall LTaebcohrnaotloorgyy and Safety Services Building 236-00 SStu.sPaanulA,.MBNeac5h5,1S0p6onsorReprasontative
Analytical ChamistryLaboratory 3`IMM EEnnvviirroonnmmeennttaall TLaebcohrnaotloorgyy(a3nMdLSaabf)ety Services (ETS) Buiding 236.09 S9t3.5PaBuuls,hMANven5u5e106 Lisa Stevenson,PrincipalAnalytical Investigator
`StuyPorsonnel S`WeJioalalnninaPm.8.K.GBaeRlalevaageghreesnr,,,WW3iibkdliEionfvoIinnrttooenmmmaaettniitoonanallaLlaLbLToTDrD,a,,t`SoLtrayubodMryaaDntioarrgeyecMtmaoenrnatgement LHSiausrsaoalAnd AOS.teBJveoeahncnshso,onSn,p,PorInil,nscoOirrpiaRglienApanrlaoPlsryoitninctcaailtpiaIvlneAvneasltyitgiactaolrInvostigalor `KOegnntjeR.nkLainKdrsuptlraonmi,n,SeArniaolrytRiacsaolaCrhcohrmCihstemist Marlene Heying, Analytical Chemist
Experimenta ston `SSaammpplleeAAnnaallyyssiiss CIonmaptloenti:on1:0F1e1bArpurary20200202 Archives wLAlaelbloorraiasgtiotnrahlyearscoacwmoprdladetiasn,gppetrrootta4oi0cnoiCln,gFaR[n0dPhaaerntaal7ny9at2liy.ctailTcharleeppoahnratalshyeativocfealtbhreseefnseracern,ccheiavsretedan&ardtcathrhidevre3edsMaerEvhneveisraomnpmleenst,ala Environmental Laboratory according to 40 CFR Part 762.
3MEnvironmenTtarl Lab-- orato-- ry.
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`SUMMARY
C4F7SO;K') was determined in the liver and sera of adult mallards and their offspring in both a
"Table 1. Summary Table, C4F1;SO5" Concentration in Sera and Liver
Group.
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107-1400Apt range (4of 18) | 11300.210000
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3M EnvironmentalLaboratory
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CNLOG8
RepertE0L-126 pe
PRA ROeproSduct:ion Study with the Mallard PROS: ARaproctonsuaveits
Table 2. Summary Table, CsF17SO5 Concentration in Sera andLiverwith Outliers Removed
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PURPOSE
TShiehpcupoomseoanfdtsheeadnalmytaiclalaprhaasned onfeirs ssups 1 quay C05 in th er and saa of
REFERENCE SUBSTANCES
i, Tomar | [-- Table 3. Reference SubstancePsros
En
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SH Labomioy
conan
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IM EnvironmentalLaboratory
TTT pages "ingg
Report EO1-1256 Aopen ov1250
PFOS: AReproduction Study with the Mallard PROS:AReprSoyditchtMoatnas
`SPECIMENRECEIPTAND STORAGE 2cMnaolndlcalsruedsnitsonetroouf3mMthaenEdnivinivreeornpmsheanamtspaelleosLfawbheoerraesttoourbdyiyafi(onr#e4da5n4fa-rly1os0mi9s)t.hcooTsnahdcourciftsiepcdeecoaiftmetWhnies dtweeestreaInnriaemrcmaealitsvienuddploa,nt LSthiMe oErnvEiDr1o-n1m3e6n7t)a,laLnadbworeartorsy,tasosaitrg2nee0ddCl/a-bor1a0toCryurnatckpirnegpa(rLeMdSfo)rannuamlybseirs(EO'-1256, EO1-1325,
METHOD SUMMARY
3ThMeEdnevtierromnimneatnitoanl oLfaCboyrFa,lSoOr;y"mceotnhcoedntrEaTtSi-o8n-2n31h.e1, Fluorochemical Compounds from Bokogical aces"
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Wpdeiaurslseeeddthsetonhrracooeungth1ri.afu0cgmeodonffdotorhne1e0hdommisonogultedensap:theathsoeef vsouextprreaarcnatdlioawnnat(sSwPsaEhs)akdceeonclaufmnonrt.e2d0 Amiinfnoeurt4e0ds.yminLTghoe(fshewoasctaeomrlpualmnends,
a2n0almyLzedoffomreCt4hFanSolO;waussipnagsHsePdLCt-rEoSuMgShItMhSe.column to clus the anaiye. The sampes were
PREPARATORY METHOD
+
bfEirToolSmo4gi.Bc2iaol3l1mo1agitci"acSloslm,iadstuiPcchheasas.se"saErTxahtiarsancdmtolitioehnro,daunsddiensgAcrnsiaoblelysspithshaesoeefxteFxritaurcaoicrotoniconohfe(mStiPaErcg)ae.lt
aCnoomlpeosnefnrtosm Sera samples
hswoaemmropegleednsiiuzwteeedrd.ewdiAih e1K.da0n1dm/i1ya0ol.hiqiaupop(trcooofxmitmmheaetreiclityael1dgbosfoervtae)orr wahanotdmeorOgmei1n/2ao0ft,eKabonefdfoiIryveoehriewxtwarasalceterix,otnr.aancdteLtdheboryn
fsauidptderirenmdgatt5ar.not0umgwhaosfaacWcoaentnodsnifitotirroirnleeed,d 1sC0h1a8aki(cnSlgPefaEon)r ct2ou0bleum,minnd.uitleAsen,dalaywntidohscew4ne0triemeuilguiotnfegdKofanofdti1hy0oehmciionulwtuaetmsen.,, aTanhndde
Sanpaalcytzoedmenbyy(HiPgLhC-EpSeMrfSoMrSma)nce laud chromelooraphy-alectospray tandem Mase
Table 4. SPE Extraction Summary
[2|MOooossovno(u2m0oaofneirmiorvweoigrhwp(wrroeino0fbr icseabolro homogerzs Neva `Aceoqvoitng1.0moS fGfoh or2dme ihns,ocsmanaonoeewfgodrS1eTmEiiOsTI.zGTAeCH Soka roles
|Dospowrih aOminhutr [6 Florsarplo rou cond SPE Coctirn
7 Ext rotary Fomibecoms20mLmeant(aOR)
3MEnvironmental Laarboratory
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Report EOL-1256 Report 011268
PFOS: AReproStduduywicthttheiManllanrd. PFS ARepaSetyicibooahnd
`SAMPLE RECEIPT, PREPARATION AND ANALYSIS
IME vr nment I Labor t 1
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