Document B8gDOepk8nELyNn1Je8VJpXVw
BIODEGRADATION (BOD/COD)
TEST SUB -TANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-603EF. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber isnot noted.The testsample is FC-603EF. Currentinformationindicatesitisa mixtureof 1.30% PFOS, 10% diethyleneglycolbutylether,54.15% water,22.5% ethyleneglycol,2.6% propanesultonefoamer,5% Alkylethersulfate0,.1% N-(3Chloroallyl)hexaminiucmhloride0,.9% Xanthan gum, 0.9% starch,1.0% triethanolaminea,nd 0.05% tolytlfiazole.
The followingsummary applies a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectdegradation of the fluorochemical component of the testsample.
METHOD:
Method: ModifiedCBOD test' Type: Aerobic GLP: No Year completed: 1996 Contact time: 28 days Inoculum: Polyseed-NX Lot# A306, Polybac Corp.,Bethelem, PA.
RESULTS
Chemical Oxygen Demand (COD): BiochemicalOxygen Demand (BOD):
5-Day:
1O-Day:
20-Day:
28-Day:
740,000 mg/kg
216,189 mg/kg 110,163 mg/kg (duplicate) 376,258 mg/kg 325,956mg/kg (duplicate) 445,674 mg/kg 438,632 mg/kg (duplicate) 601,610 mg/kg 317,907mg/kg (duplicate)
Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
No conclusionsgiven.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 3. Study lacks description and records of the method followed.The inoculum was not propedy characterized.Sample puritywas not propedy characterizedand the study lacksanalytical confirmationofthe amount of fluorochemicalproportioninthe solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St.Paul,MN, Lab Request number P2581, 1996.
OTHER
Last changed: 6/26/00
Sf--CONt@R@UN
Modified CBOD Test 5,10,20 & 28 Day Analyses
Sample Descriptionr
CF
Concentrationof stock solution
pH Stock Solution
Polyseed -NX Lot
LR#
Date__l I
Analyst
\,(-'3
_Mg L
LR#
Date
'i@
Modified CBOD Test 5, 10, 20, & 28 Day Analyses
Sample Description
Bottle A Day
33 0
34
0
3G
37
5
38 5
39 5
40 5
41 10
42 10
43 10
44 10
45 20
46 20
47 20
48 20
49 28
50 28
51 28
52 28
Sample Vol.
(mg/L)
Sample conc. (mq/L) Li
9 Li Y.
Li It
Initial Final
Dissolved Dissolved
Oxgyen Oxgyen
(mq/L
m /L
Date Initials
-i(, 67)-
ie-
Li
L/ C't. c@-gq d 14.8' L4C?4 g.c?q
q. 13
v
/I-i:2 olc.
-illo
1.2
-I-ie.6
0.12- 1.2 1.2-1 (C- 1 V-.5
i-2-.7e-)?c; 15 /1@-.20
Modified CBOD Test 5, 10, 20, & 28 Day Analyses
Sample Description
LR# Date
BottleA Day
5.13 0
54
0
55 0
56 0
57 5
58
5
59 5
60 5
61 10
62 10
63 10
64 10
65 20
66 20
67 20
68 20
69 28
70 28
71 1 28
72 28
Sample Vol.
(mg/L)
Initial Final
Sample Dissolved Dissolved Date conc. Oxgyen Oxgyen (mg/L) (mq/L)i m /L Lt.C-l1
lnitlais
Lfcl7
j/ Ile 6
;/
P3;lj-
1
Tv-@,
2@-4
-C)
qg.
'7
A@.
911/ q.
L)-7 -144 -W Li 0. oc-
O,C,5
4 0
19-3"1 L.@7 7
1.7-1 -c?
Ic-
Product Testing ModifiedCBOD Test 5, 10,20 & 28 Day Analyses
Mean CBOD mg/Kg
CBOD Day Results (mcl/kq)
0
0
235412 5 240443 5 216.189 191935 5 145070 5 301811 10 376,258 376258 10
-261290 10 130986 10 362173 20
.674 445674 20 245968 20 123340 20 601610 28
601.610 510060 28 205645 28 103219 28
CBOD (mg/kg) Samp DO Drop
Dii Bottle sew
No. No.
Vol
(mL)
1 33 5 2 34 5
35 5 36 5 1 37 5 2 38 5
3 39 5 4 40 5 1 41 5 2 42 5
3 43 5 4 44 5 1 45 5 2 46 5 3 47 5 4 48 5 1 95 2 50 5 3 51 5 4 52
(mg/L)/ Samp Conc
P2581 FC-603EF
Sample DO Dro
Initial Final DO Drop
lnitamlinus
samp Samp Seed Dissolved Dissolved Initimailnus
Finalminus
Vol Cone. Corr. Oxygen Oxygen
Final
Seed Corr
(mL) JmQ/L) Factor (mg/L) m L
mL
m /L
5 4.97
8.45
10 9.94
8.46
25 24.8
50 49.7 5 4.97 1.12 10 9.94 1.12 25 24.8 1.12 50 49.7 1.12 5 4.97 1.86 10 9.94 1.86
'@6.16 ...!.2.29
4.95
3.51
2
2.58
5.88
@,.,.:0.13 8.33
.7
.09
2.86
5.60
3
25 24.8 1.86
50 49.7 1.86 5 4.97 2.24 10 9.94 2.24 25 24.8 2.24 50 49.7 2.24 5 4.97_ 3.24 10 9.94 3.24 25 24.8 3.24
49.7 3.24
0.12
8.34
0.09
8.37
6.
4.41
4.04
1
1.79
6.67
4
8.34
6.
:0@09
8.37
6.
2.22
6.23
2
olisl 0
8.311
0 'IA
0.091
8.371
(mg/L)) X 1,000,000mg/kg
Greyed areasaredatawithunacceptableoxygen concentrationsF:inalD.O. must be @!0.5mg/L,and
Sample DO. drop (initi-aflinal-seedcorrectionm)ust be @!2.0mg/L forthedatato be used to calculatCeBOD.
Product Testing Modified CBOD Test 5, 10, 20 & 28 Day Analyses
Mean CBOD mg/Kg
110,163 325,956 438.632 317,907CBOD (mg/kg)
CBX
Day
Results
(mg/kg)
Oil Bottle Seed No No. Vol
(mL)
0 1 53
5
0 2 54
5
0 3 55
5
0 4 56
5
90643 5 1 57
5
105634 S. 2 58
5
102419 5 3 59
5
117907 5 4 60
5
211268 10 1 61
5
325956 10 2 62
5
262903 10 3 63
5
130181, 10 4 64
5
295775 20 1 65
5
438632 20 2 66 -5
247581 20 3 67
5
122938 20 4 68
5
255533 28 1 69
5
317907 28 2 70
5
206452 28 3 71
5
101811 281 4 1 72 5 Samp DO Drop (mg/L) / Samp Conc
P2581 FC-603EF Duplicate
SImp Vol (ML) 5 10 25 50 5 10 25 50 5 10
Samp, Conc. m g,L 4.97 9.94 24.8 49.7 4.97 9.94 24.8 49.7 4.97 9.94
Seed Corr. Factor
1.12 1.12 1.12 1.12 1.86 1.86
Initial Dissolved Oxygen
(mg/L) 8.45 8.44 8.44 8.40
25 24.8 1.86
so 49.7 1.86
5
4.97 2.2244
10 9.94 2.24
25 24.8 2.94
50 49.7 2.24
5 4.97 3.24
10 9.94 3.24
25 24.8 3.24
30 49.7 3.24@@ (mg/L) X 1,000,000mg/kg
Final Dissolved Oxygen
mL
DO Drop Initimailnus
Final mL
Sample DO D lnifimailnu Finaln@nus Seed Corr
:6.88 6.27 4.78 1.42 5.64 3.34 .,.0.06 :.0.07
1.84 0.06 0.05 .3.94 2.04 0.08
:1.57 2@l 1 3.66 6.98
5.10 8.38 8.33 @3,71 6.60
8.35 4.5 6.40 8.36 8.301
Greyed areas aredatawithunacceptableoxygen concentrations:FinalD.O. must be Z!0.5mg/L, and Sample D.O. drop (initi-aflinal-seed correctionm)ust be > 2.0mg/L forthedatato be used to calculateCBOD.
ModifiedCBOD Test 5, 10, 20, & 28 Day Analyses OC Data
LR# Date -.-rr%a:-tr-o Analyst (33-1 1 Tic-S
Sample Description
Medium Blank Medium Blank SeededBlank Seadf I'@ Rot. Std.GGA Rai. Std. GGA Tox. Check LR# Tox. Check LR# Tox. Check LR# Tox. Check LR#
102519 1 f,2q5 (o P,2Q5@
Imnni0tIaviLal
Final
Bottle liDDoi'OXsW"sNoldvedDissolveDd,,iDt atae
Initial&
Oxgyon
Oxgyon
(mq/L)
m /L
I
I
<. Z/&
it-a2-I# ppil-
2
q7
3
4
5
rt. q5
6
7
5f.'Y7
say
8
)T.q
sItT
9
10
Dav 5
Medium Blank
11
Medium Blank
11 2
Seeded Blank
13
Seeded Blank
14
Ref.Sid.GGA
1505
Ref.Std.GGA
166
Tox. Check LR# E!.2581
17789
Tox. Check LR# 1?:115b
18
Tox. Check LR# P;k959
19
Tox.Check LR#
E 22 0
Dav 10
Medium Blank
21
Medium Blank
22
Seeded Blank
23
Seeded Blank
24
19.13
'7.44 sit,4
If- 2.7-q(o 1 ?--7-91(. 11-2--y-9r.
-rk5 Tics 'TtIS
0. 1!5 0. IZ
Oc'T P u k)
I I-z7-9r.
-% -I*X
a@r 5.13
W-T
I;L-;4t(o
AiJ
Dav20
Medium Blank
25
Medium Blank
26
SeededBlank
27
Seeded Blank
28
C-03 7.0(I..'X-4 (c -2 o
L/-I-
-r<
12-tz.-Itc" -%
1.7-12.-Ctro ,if-
',a-1 2--C?r.
Dav 28
Medium Blank
29
Medium Blank
30
Seeded Blank
31
Seeded Blank
32
7 '1 1 T 94 ;s @z)
5 ib
-30 -.7c-,7c,
167 --lo -lt6i .2-ae- I
-Tt< 5 -1L-5 -%t-5 -Tie-5
Modified COOD Test 5, 10, 20. & 28 Day Analyses
OC Results
Description
CBOD mg/
Bottle
lnitia dissolved
oxgyen (mq/Ly_
LRO
Date
+2
Analyst
Final dlssolv*d
oxygen (mq/L)
00 drop Initial-
Final (ma/L)
DO drop InitialFinalseed car (mq/L)
5 Dav OC
Medium Blank
11
Medium Blank
0
12
Seeded Blaannkk@
13
Seeded Blannkk
14
Ret.Std.GGA
298
15
Rof. Std.GGA
326
16
Tox. Check LR#P2581 >1000
17
Tox. Check LR# P2956 > 1000
18
Tox. Check LR# P2958 > 1000
19
8.46 8.47 8.47 8.45
8.4S 8.47 8.47 8.47 8.44
8.04
0. 2
8.13 7.22
52gaA 0.l3@4
1.
IL
1C',SCELJ
S10..125
7.47
0.98
0.98
mean SCF= 1.12
5.84
2.61
1.49
5.72
2.75
1.63
0.17
8.30
7.18
0.15
8.32
7.20
0.13 1 8.31 1 7.19
10 Day OC Medium Blank Medium Blank Seeded Blank Seeded Blank
20 Day OC Mediu 1 k @2 Medium BB'llaaa@nk Seo"d,d@BBllaanankk Seededd Blankk
28 Day OC Medium Blank Medium Blank Seeded Blar* Seeded Blank
,(Seed Control Factor)
20
8.46
21
8.47
22
8.47
23
8.45
44
8.46
255
8.47
266
6.47
277
8.45
28
8.46
29
8.47
3
8.47
0
8.45
8.13 8.12 6.65 6.56
8.08 8.06 6.24 6.20
7.81 7.84 5.30 5.15
0.33 0.35 1.82 1.89
rpranSCWF.
SCFI 1.86
0.38 0 -.348 E 20!2!3215i1 20.25
ffeanSRCFmean SCF=
S F* E2S32.23
F. 2.26
26 2C.-2244
0.65 0.63 3.17 3.30
;,an
SCF*
3.30 SICF.%3.24
Tox. Checks containssample at the highestconc. used for dilution&s 1.5 mLs of HACH GGA Sid
Seed CorrectionFactor(6WL)= Seeded Blank DO Drop Rot.Sid.BOD (mg/L)z(Ref.Std DO drop-Seed CorrectionFactor)X (300mL/ Rof Std vol (mi))