Document B8KyDR0RGydM8EEz4rQ73L794

.4 ^ n u i . ; >*:: E. I. d u P o n t d e N e m o u r s S C o m p a n y INCORPORAftO W i l m in g t o n . D e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT EXPCRi i'r m f lL s ta tio n ) PERSONAL AND CONFIDENTIAL cc: A. J. Dali! B. V. Karrh L. J. Papa Pral File 1 -C. 333 NI1400 269 -^/226-/5gS July 16, 1981 DR. J. E. STOUT PHOTO PRODUCTS BREVARD, N.C. ANALYSIS OF BLOOD SAMPLES FOR PERFLUOR00CIAN0ATE (Job No. 811-681; PRAL Kos. 81-2535-2540; Notebook Nos. E22514,E26238) As requested in your letter to L. J. Papa, the 3 blood samples received 6/4/81 have been analyzed for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm E for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of 'u 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Words: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC S. S. Stafford Ti.f-re * a world of things we To doing borvlhm c about 000104 EXP000040 EID713854 TABLE I CONCENTRATION OF PEKFLUOROOCTANOATE IK BLOOD (a) Sample (c ) PRAL No. Date Sampled 81-2535 81-2537 81-2539 6/2/81 6/3/81 6/3/81 P.R.No 5100 WSR 5130 Name GC Analysis _____________ ( Date Analyzed Cr), _V F/g bio 6/19/81 n.d . 6/19/8 L n.d. 6/19/81 n.d. (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 ygF/g. The detection limit is n, 0.004 pgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] 0.004 ppm, which cannot be distinguished from reagent background. (c) Two samples of blood were received for each individual, one taken in a heparinized collection tube (81-2535,2537, and 2539) and a second taken in an EDTA collection tube (81-2536,2538, and 2540). Analysis was dene on the heparinized blood samples. REDACTED* 000105 EXP000041 EID713855