Document B8GG8p8O24EM5OBrrnyN53OrL

BACK TO MAIN TOXICITY TO FISH (Freshwater Rainbow Trout) TEST SUBSTANCE_________________ Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder of uncharacterized purity. METHOD___________________________________________________ Method: Standard procedures for Testing Acute Lethality of Liquid Effleunts (Environment Canada, 1980) Type: Acute, renewal after 48-hours GLP: No Year completed: 1985 Species: Rainbow Trout Fish source: Rainbow Springs, Thamesford Fish age at test initiation: not noted Exposure period: 96-hours Analytical monitoring: Dissolved oxygen, pH, conductivity Statistical methods: Not noted. Element values were calculated for each replicate series, but not combined for the whole study. A cumulative mortality-concentration plot was used to estimate the LC50. Test conditions: Dilution water: Mississauga dechlorinated tap water Dilution water chemistry (initial): pH: 7.5 - 8.5 D.O.: 9.0 - 10.4 mg/L Stock solution preparation: 1000 mg/L; noted as cloudy Exposure vessels: Not noted; solution volume 35 L Number of replicates: 2 tests - run one week apart, not replicated Number of organisms/vessel: 6 Loading: 0.72 g/L Number of concentrations: 5 plus a blank control, 4 plus a blank control Water chemistry during the studies: Dissolved oxygen ranges 8.2 - 10.4 (control) 8.0 - 9.5 (30 mg/L) pH ranges 7.4 - 8.3 (control) 7.5 - 8.7 (30 mg/L) Conductivity range: 270 - 380 gmhos/cm BACK TO MAIN Test temperature (0 - 48 hours): 15C Element basis: mortality RESULTS________________________________________________ Element values (95% confidence interval) calculated per replicate: 96-hour EC50 = 7.8 (6.2 - 9.8) mg/L 96-hour EC50 = 9.9 (7.5 - 13.4) mg/L Mortality of controls: None DATA QUALITY___________________________________________ Reliability: Klimisch ranking = 2. This study satisfied all criteria for quality testing at the time performed, but actual concentrations were not measured. Results were based on nominal concentrations. Additionally, sample purity was not adequately characterized. REFERENCES_____________________________________________ This study was conducted by Beak Consultants Limited, Mississauga, Ontario, Canada for Panarctic Oils Ltd, Calgary, Alberta, Canada. OTHER_________________________________________________ Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 6/12/01