Document B8BxYYK8Bbk56dR2rvOEG89mk

"/\fL S l.olG -- D & 3> ACUTE TO XICITY TO AQUATIC INVERTEBRATES (ACARTIA TONSA) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfbnated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect flue toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: ISO/DIS 14669-1997: W ater quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). Type: Acute static GLP: No Year completed: 2000 Species: Acartia tonsa Supplier: Test organisms cultivated from eggs supplied by Marine Biological Laboratory, University of Copenhagen, Denmark. Analytical monitoring: pH and dissolved oxygen Exposure period: 48-hours Test organism age: Large copepodites and adults Statistical method: LC50 values calculated by PROBIT analysis, National Swedish Environmental Protection Board (Version 2 .3 ,1 9 9 0 ). Calculation of NOEC by single factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord outside of Stavanger, Norway. Dilution water chemistry: Not given. Lighting: A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 999.4 mg/L primary stock solution was prepared by diluting 0.4997 grams of test substance in 0.5 L of water. Aliquots were then added directly to water in test vessels to create test solutions. Exposure vessels: 28 mL rolled rim vials containing 25 mL of test solution Number of replicates: 4 000231 Number of animals per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 8.1 - 8 . 1 (control exposure) 8.0 - 8.1 (100 mg/L exposure)* Temperature range: 20 1 C Dissolved oxygen range (0-48 hours): 7.2 - 7.6 mg/L (control exposure) 5.6 - 7.7 mg/L (100 mg/L exposure)* * 100 mg/L (intermediate concentration) exposure values used because the highest two concentrations used resulted in total mortality by 24 hours. RESULTS____________________________________________________ Nominal concentrations: Bk control, 10, 32, 100, 320, and 999 mg/L. Reference substance (3,5-dichlorophenol) at 1.0 mg/L. Element values: 24-hour LC50 = 52 (37 - 71) mg/L 48-hour LC50 = 34 mg/L (Cl not calculable) 48-hour NOEC = 10 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS _________________________________________________ The FC-201AF 48-hour LC50 was determined to be 34 mg/L. The 48-hour No Observed Effect Concentration was 10 mg/L. Validity criteria fulfilled and reference substance was in normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ 000232 Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-1, 2000. OTHER___________________________________________________________ Last changed: 6/26/00 000233 L99a3b1.r2e-f1.no.: Page 1 o f 31 IS) AKKRNEODRTSTKER1NG ROGALAND RESEARCH Nr. pm * "FC-201 AF Light Water Brand AFFF" Toxicity tests Report RF-2000/001 Lab. ref. no.: 99312-1 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1/05.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-201 AF Light W ater Brand A FFF ': Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC* 48 h LC^ 10 d LC (mg/l): ~50 (m g /l): 34 (mg/kg dry weight): >3020 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced in any form without permission in writing by RF - Rogaland Research . V Inger Lisa Andersen a**-- for RF - Rogaland Research 000234Kre Netland L9a9b31.r2e-f1.no.: Page 2 o f31 CONTENTS: 1. Preface................................................................................................ 3 2. Test for toxicity with Skeleto nem a c o sta tu m ....... ....................................................................4 3. Test for toxicity with A cartia tonsa...............................................................................................10 4. T 15est for toxicity with C o r o p h iu m v o lu t a t o r ............................................................................. 5. Datasheet^ ) ................................................................................................................................................20 000235 L99a3b1.r2e-f1.no.: Page 3 of 31 PREFACE: Ecotoxicological tests were Novatech. The sample is a performed on "FC-201AFLight concentrate that normally will bWeautesreBdriannad AFFFnin agreement with 1% solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 / SFT1998). Theproject was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Miljolab. Study director was Senior Engineer Inger Lisa Andersen. The test results arepresented in separate sections. The results relate to theproduct as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and workingproceduresfor all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in.interlaboratory comparisons, and the use of certified reference materials and secondary reference materials. The testsperformed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without thepermission in writing by RF - Miljelab. 000236 L99a3b1.r2e-f1.no.: Page 4 of 31 T est for T oxicity Marine alga: Skeletonema costatum 000237 L9a9b3.1r2e-f1.no.: Page J o f 31 TEST REPORT Ga lRgOaWTSHkIeNlHeItBoInTIeOmN TaEcSTosWtIaTtHuTmHE MARINE (Sl NORSK AKKREDITERING Hi. POI Hitii RESEARCH TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The 72 hours EC50was calculated to ~50 mg/l. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NwSit-hESNkeIlSeOton1e0m2a53c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-ctMylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, strain NIVA BAC 1. A stock culture of the test organism is kept at RF - Rogaland Research. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the growth medium. The water was filtered through a GF/C filter, and briefly heated to about 75C prior to use. The seawater was enriched with nutrients according to ISO 10253, and recommendations from the Paris Commission. Test solutions were prepared from a stock solution. A stock solution of 511.2 mg/l was prepared by stirring 0.5112 grams of the product in 1 litre of growth medium. Other concentrations were prepared from this stock solution. INCUBATION: ToTlarehtbseeitdttacetluossltthauwbarkaoeusesrtro,u2f3n050ru0e0npmdclieelcrlawlscte/eomrsnefltoiifnnrrucoeuomabucashatnlecidgoehnxintcpe(o1n>n0ter50na0mttiipoalEnlfl.ly/antgTr-r-besosoewttktci)onumfglrtueoindrmeofsclfaulwusluekormrseeosicncnueoalntcnuture. tubes of universal natural white type, at a temperature of 20 2C. Tbthyheeftletuesostrtfewlascaseksnsrcueena.cfhpoHrdaambyoefuaostru7gr2reomhwoetunhrtssm,waeanersduesrpueembrsfeaonmrtmsp.elAedslgaosafli5ngdmrioclwawttehedrweoantsadkmaenetaafssruhoremeedt. 000238 L9a93b1.r2e-f1.no.: Page 6 of 31 DATA ANALYSIS: CALCULATIONS: vGerroswusthtirmatee,saws edreesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3r.ession on biomass (fluorescence) ErCso: The inhibitory effect on the growth of Skeletonema costatum is expressed as the concentration of a product giving 50% effect concentration was calculated by reduction of interpolation gbreotwwtehenra3te2m(EgrC/l 5aon).d This 100mg/l.. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by singlefactor ANOVA followed by Bonferronis procedure for comparing each concentration mean v/ith the control meanr TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance BrC,o ErCjo EC7 90 "WFaCt-e2r0B1raAnFdLAiFghFtF" - ,. ~50 _ NOEC: 10 GVVaarlaluupeehssPainarddeiIccnaaStlectauctloa3nt.ce0ed. nbtryaitnitoenrspogliavtiinogn.10N%O. E50C%araencda9lc0u%laitnedhibbyitiuosninogftghreowcothmprauttee.r pUronigtrsaimn mmge/1. The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor of more than 16 in the test period (corresponding to a growth rate of more than 0,9 d`l), the control pH did not vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. LSaamb.prleef.rnecoe.:ived.: TReesptoprterdiaotde:: 2909.30192.9-19 0045-0.071.1.200.9090 Elin Horve Engineer Analytical technician SenQiAourgaRelieMtsyeoAalvrsceshrusrSmacnyicerent]_ist Enclosed: Data sheets Tfohrims toesthterrepthoarnt iisnisfsuulle,dwbiythaoulat bthoeraptoerrmy iascsciroendiitnedwrbiytinNgobrwyeRgFian- MAiclcjoreladbi.tation (NA). The report may not be reproduced in any 000239 L99a3b1.r2e-f1.no.: Page 7 o f31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 Start date: 04.10.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence; values in fluorescence units Time: 04.10.99 05.10.99 06.10.99 07.10.99 Concentration 14:05 09:25 10:30 10:40 Growth PH pH rate Corre- at start at end day-1 lation of test of test 0 0.11 0.59 9.2 56 2.18 0.997 0 0.15 0.63 7.9 59 2.09 0.999 0 0.13 0.62 9 54 2.11 0.998 8.3 8.4 0 0.14 0.63 6.4 47 2.04 1.000 0 0.14 0.62 6.3 48 2.04 1.000 0 0.12 0.71 7.1 55 2.14 1.000 0.1 0.16 0.74 7.2 60 2.07 1.000 0.1 0.13 0.61 5.5 55 2.12 0.999 8.4 8.5 0.1 0.14 0.55 8.3 64 2.14 0.998 0.32 0.32 0.32 0.14 0.14 0.14 0.74 0.68 0.68 8.7 8.6 9.2 58 63 62 2.11 0.999 2.14 0.999 2.13 0.999 8.4 8.4 1 0.14 0.83 8.2 59 2.11 1.000 1 0.15 0.88 9 61 2.10 0.999 8.4 8.4 1 0.13 0.93 8.9 61 2.15 0.999 3.2 0.14 0.86 9.2 67 2.16 0.999 3.2 0.16 0.76 8.9 73 2.14 0.999 8.4 8.5 3.2 0.15 0.63 7.3 63 2.11 0.999 10 0.16 ' 0.45 5.5 57 2.06 0.994 10 0.15 0.46 5.2 52 2.05 0.996 8.4 8.5 10 0.16 0.46 6.2 49 2.00 0.995 32 0.14 0.4 3.4 25.5 1.82 0.996 32 0.15 0.3 2.7 17 1.66 0.991 8.4 8.4 32 0.13 0.4 4.4 33 1.94 0.997 100 0.15 0 00 0.00 100 0.18 0.01 0.03 0 0.00 100 0.13 0 0 0 0.00 8.4 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.14 0.47 1.85 1.5 mg/l 0.16 0.43 1.45 1.5 mg/l 0.16 0.45 1.45 4.2 3.3 2.9 1.19 0.992 1.06 0.996 1.01 0.992 8.3 8.2 2.10 2.11 2.13 2.12 2.14 2.04 1r\.80 0.999 0.999 0.999 0.999 1.000 0.995 0.995 0 0 2 4 CThe result of t h ^ ) reference L9a9b3.1r2e-f1.no.: Page 8 of 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 8C 8 5 o. 13 i J 1-1? K-3 100 mg/1 24 48 72 test duration (hours) 0 0 0 2 4 1 L9a93b1.r2e-f1.no.: Page 9 of31 Inhibition of the growth of Skeletonema costatum with: mg/1 ("FC-201 AF Light Water Brand AFFF") Experimental data: Concentration in Growth rate "FC-201 AF Light Water Brand AFFF" Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.10 0.10 0.10 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 2.18 2.09 2.11 2.04 2.04 2.14 2.10 2.07 2.12 2.14 2.11 2.14 2.13 2.11 2.10 2.15 2.16 2.14 2.11 2.06 2.05 2.00 1.82 1.66 1.94 0.00 0.00 0.00 Inhibition in percent - - 0 1.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.9 2.4 4.8 13.3 ? , 21.0 7.6 100.0 100.0 100.0 000242 L9a9b31.r2e-f1.no.: Page 10 o f31 T est for T oxicity Marine herbivore: Acartia tonsa 000243 L9a9b31.r2e-f1.no.: Page 11 of 31 TEST REPORT ACUTE TOXICITY TEST WITH Acartiam a r in e copepo d TtoHnE sa (Ql NORSK AKKREDITERING RROE GS EAALRACNHD TEST SUBSTANCE: "FC-201 AF Light W ater Brand AFFF" TEST RESULT: The 48 hours LC50was calculated to 34 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ISO/DIS 14669 - 1997: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). TEST ORGANISM: Acartia tonsa (Dana), supplied by the Marine Biological Laboratory, University of Copenhagen. Cultivation: Twhitehttehset o"rPgaarnisisCmosmwmerisesisounpp- lRieidngasTeegsgt sP,raontodcwole"re(VcKulIt)i.vaLteadrgine caocpcoeprdoadnitcees and adults were used in the test. PREPARATIONS: Npasraitbourarstaiosl usfeosaerw.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmatearnwunaps ofillltuetreeddstihteroinugBhyafjGorFd/Cwafisltuesred Tmdieglsu/1ttiswoonaluswtpiaortneeprs.awrOeedrtehbepyrrscetopinrarrcieendngtfr0rao.4tmi9o9na7sswgtorecarkme ssporoleufpttaihorenedp. rfAordosmutcottchikniss0os.tl5uoctliikotrnseooolufft9io9n9..4 INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml of test solution and 5 animals, 4 replicates for each concentration. The vials were kept under a 16h/8h light/dark photoperiod at a temperature of 2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the 000244 tnumnrtiM' Nftrwnv Tal.: +47 51 87 50 00. Fax: +47 51 8752 00. Enterprise number.: 950884800. L99a3b1.r2e-f1.no.: Page 2 o f31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Acartia tonsa is expressed as the concentration of paeprrtoedsutcctolnectehnatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4cohmoubrisnaenddm4o8rthaoluitrys LstCa5n0davradlupersocaenddutrheeP9R5%OBcIoTnfAidneanlycseisli,mNiattwioenrael calculated by using the Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfaacltcourlaAtNioOn VofANfOolEloCw(eNdobOy bBsoenrfveerdroEnfifsepctroCcoendcuernetfroarticoonm) ipsadroinngeebaychsingle concentration mean with the control mean. Test RESULTS: Table 1. LC and NOEC values obtained with:"FC-201 AF Light Water Brand AFFF' Acartia tonsa 24 hours 48 hours LCio 20 (10-29) 12 ( - 29). L C jo 52 (37-71) 34 ( - ) LC$o 130 (92-260) 93 (39- ) NOEC: 32 1 0 ctVhaaellcucueolsmaitpneuddtibecyraptPerRo1gO0r%Ba,ImT5m0ae%naGlayrnsaidps,9h0aP%naddl9Ien5thS%atalcittoy3n.a0fif.dteern2ce4 ianntedr4va8lhsoaurres.indUicnaittsedin. mNgO/lE. CVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 4 mg/1, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 o f 3,5-dichlorophenol (reference) was in the range normally found. LTSRaaeembspt.opprrleteefrd.riaonetdcoee:.:i:ved.: 0065-20.900891..301.219002..990-1990 Elin Horve Age Molversmy`rV Engineer Senior Research Scientist Analytical technician Quality Assurance Enclosed: Datasheets Tfohrims toesthterrepthoarnt iisnisfsuulle,dwbityhoaulat bthoeraptoerrmy iascsciorendiintedwbriytinNgobrwyeRgFia-nMAiclcjereladbit.ation (NA). The report may not be reproduced in any 4 000245 L99a3b1.r2e-f1.no.: Page 13 o f31 PRIMARY DATA: Acute toxicity te s t w ith Acartia tonsa Test substance: "FC-201 AF Light W ater Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 06.10.99 Number of dead Concentration (mg/!) Control 10 32 100 320 999 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 555 30 5 5 5 555 20 5 555 20 5 5 55 20 5 555 20 5 555 20 5 NUMBER OF DEAD 24 hours ACE B DF Total 48 hours ACE BDF Total 0 00 0 01 001 0 0 20 1 4 44 5 -0 0 0 1 101 1 11 10 121 31 5 55 17 5 2 3 -5 20 5 55 555 5 20 5 20 5 55 5 55 5 20 5 20 - Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 0 20 2 54 30 5 55 100 3 130 The result with the reference substance is within the range normally found. 15 t 000246 L99d3b1.r2e-f1.no.: Page 14 o f 31 Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: 99312-1 06.10.1999 Measurements of oxygen and pH. Start Concentration (mg/l) Oxygen (mg/l) pH 24 hours 4E hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.6 8.1 7.2 10 7.6 8.1 7.0 32 8.1 8.1 6.5 100 7.7 8.1 5.6 320 7.7 8.1 6.6 8.1 999 7.5 8.1 6.5 8.1 pH 8.1 8.1 8.1 8.0 Reference substance (3,5-dichlorophehol): 1.0 mg/l 7.7 8.1 7.3 8.2 000247 L9a93b1.r2e-f1.no.: Page 15 o f 31 T est for toxicity Marine Amphipode: Corophium volutator 000248 L9a9b31.r2e-f1.no.: Page 16 of31 TEST REPORT AMCAURTINEETAOMXIPCHIITPYOSDEDCIMorEoNpT hTEiuSTmWVITOHlTuHtaEtO V (5 NORSK AKKREDITERING RR EOSGEAAI AR NC HD TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The lOd LC50was calculated to >3020 mg kg'1 dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E l367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. TEST organism: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount of natural seaweed was used for feeding. PREPARATION: NwSantaaaatsvulyaursnaselegddeserffaowowrraaopstrreugeraspe(nasdiracilfniocngorinttdyetis:eltnu3stt4eiaod%nnidmow)peafnrrtoetosprm..orTaStniheodeuninsmsepidleotnilmlftuocertenoedltaleiscscihttaeetdeminstau.Btdaydnfyjousrnadnpedonlaluonutdetdswidsaieste Each test concentration was prepared by adding the product in 600 grammes of damp sediment in a bucket was shaken for p3lahsotuicrsb.uTckheet.se2d0im0 emnlt of seawater was added was divided into three and the beakers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of the damp sediment was analysed for water content, and all concentrations are calculated as mg kg' 1 sediment dry weight. INCUBATION: The test was run in glass containers (600 ml) each containing 200 g of test sediment, app. 400 ml of seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a tienmdipceartaetduroenothfe15data1shCe.etp. H and oxygen measurements were performed as j i 000249 ^GER: Professor Olav Honssensvei 15, P.O. Box 2503 Ulandhaug, 4091 Stavanger, Norway. Tel.: +47 51 87 50 00. Fax: +47 51 8 7 5 2 00. Enterprise number.: 950884800. ti------ 55 500ft Barden. Norway. Tel.: +47 55 54 38 50. Fax: +47 55 54 38 40 L9a9b31.r2e-f1.no.: Page 17 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen tvmraaolturitoeanlaiontydf patehprerto9ed5sut%cctoclnoecntehfnaidtlretanotci5oe0nl%iwmeoirtfeatruheesectdaelsfctouarlnacitameldcaulbslya(tLuiosCnin5.0g)T.thhTeehs1et0acndodamayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfaacltcourlaAtiNonOoVfANfOoElloCw(eNdobOybBsoenrvfeerdroEnfifsecptrCocoendcuernetrfaotriocnom) ipsadroinnge beaycshingle concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with: "FC-201 AF Light Water Brand AFFF' Corophium volutator 10days LCio 800 LCjo >3020 LC*) >3020 NOEC: 495 GVVaaralluupeehssPianarddeiIccnaaStlectau1tl0a3%t.e0,d. 5b0y%PRanOdB9I0T%anlaeltyhsailsi.tyNaOfteErC1a0rdeacyas.lcuUlantitesdibnymthgekcgo'1mspeduitmerepnrtodgrryawmemigeht. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the percentage mortality in the controls were less than 15% and a test with Fluorantene (reference) was in the range normally found, but the dissolved ocoaoxcfncytechgpeeentnretedrcpaolltiniimcocaneittnsests(rf3oaa0rtfi2toeo0rnrgmtweangnasikdcglaec*yso1ssnd. ttrehTynahwtnebersuiegetcdhotithmmesmeesdnielitntmudfesreneaddtc)t(ii6aos0fnftoe%irus)nfridievncetooodmnbaemeysoewfnaitndthhdeeidnitnhesiogtnheer. Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0.8 LSaabm.prleef.rnecoe.:ived.: Test period: Report date: 99312-1 20.09.99 03-13.12.99 0 5 .0 1 .2 0 0 0 Elin Horve Engineer SeniAorgRe eMseoalvrcehrsSmcyierntist Analytical technician Quality Assurance Enclosed: Datasheets 000250Tfohrims toesthterrepthoarnt iisnisfusulle,dwbityhaoulatbthoeraptoerrymaiscsciroendiitnedwrbiytinNgobrwyeRgFia-nMAiclcjareladbit.ation (NA). The report may not be reproduced in any L99a3b1.r2e-f1.no.: Page 18 of31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 03.12.99 Number of dead Total number Number of specimen pr. vessel NUMBER OF DEAD 5 days 10 days Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F ACE BDF Total ACE BDF Total Control 10 10 10 60 10 10 10 .0 0 0 000 0 92.4 10 10 10 30 100 1 10 10 10 495 30 0 11 2 1030 10 10 10 30 121 4 3020 10 10 10 30 122 5 - Reference substance (fluoranthene): 10 10 10 20.3 30 3 22 The result with the reference substance is within the range normally found. 7 000251 L99a3b1.r2e-f1.no.: Page 19 o f31 Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: 99312-1 03.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) <%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 92.4 94 8.1 76 8.1 495 94 8.1 91 8.1 1030 95 8.1 87 8.0 3020 97 8.1 49 7.8 10 days Oxygen (%) pH 91 8.0 88 8.1 89 8.1 89 8.1 77 8.0 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 000252 L99a3b1.r2e-f1.no.: Page 20 of31 D ata Sheets 000253 L9a9b3.1r2e-f1.no.: rge i uj Ji H.MS-DAT3IAD (Kelse-, Mil jo- og Sikkerhetsdatablad) Dokumentr.: Erstatter : 10-4145-6 Utgitc : 11/11/98 Versjon - : 06.01 03/11/93 Skrevet ut : 04/01/99 Side 1 av 11 KMS-databladet er utarbeidet av Miljoavdelingen ved 3M Norge A/S. Telefon: 63 34 75 00 / Telefax: 63 84 17 88 1 IDENTIFIERSJON AV KJEMIKALIST OG ANSVARLIG FIRMA. Importar: 3M Norge A/S Hancelsnavr. : FC-602 LIGHT WATER 3RAND ATC PLUS Deklaras jonsr.umraer, Produktregisteret : ?-nr.: 035337. Telefon, Giftinformasjonssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJEMI5X SAMMENSETNING. Informasjon on enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VANN 7732-18-5 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 2-(2-3UTOKSYSTOKSY)-STANOL / 112-34-5 (DIETYLENGLYXOLMON03UTYLETER) Faresymboler/risikosetninger: Xi; R: 36 FLUORALKYL OVERFLATEAKTIVE STOFFER Trade Secret SYNTETISKE OVERFLATEAKTIVS STOFFER Trade Secret MAGNESIUMSULFAT 7487-83-9 TYXNERE Trade Secret RESTER AV ORGANISKE FLUORKJEMIKALIER Trade Secret VEKT-P ROSENT 58 -- 63 SS 20 SS 12 1-5 1- 5 =1 0.5 - 1.5 Ikke kjent 000254 L9a9b31.r2e-f1.no.: Page 22 of31 FC-602 LIGHT WATER BRAND ATC PLUS 3 VIX7IC-3TE FAREMOMSNTER. viktigste skadevirkninger Kelseiare : Kan irritere eynene, huden og luftveier.e. Sikkerhet: Miljofare: Soesialavfall. 4 FORSTEEJELPSTILTAK. Tiltak syne : Skyll straks ayner.e med rikelige mengder var.n. Kontakt lege umicdelbart. Ti Irak hud: Skyll huden med rikelige mengder vann. Kvis irritasjonen vedvarer, kontakt lege. Tiltak irnancing: Kvis symptomer oppstir, bring vedkommende bort fra eksponeringskilden. Sarg for frisk luft. Hvis symptomene vedvarer, kontakt lege. Tiltak svelgir.g: Kontakt lege umiddelbart. Fremkall brekninger kun i samrd med medisinsk personell. Gi aldri noe gjennom mur.nen til en bevisstlas person. 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: 00255 Lab.ref.no.: 99312-1 rage 2 oj 21 FC-602 LIGHT WATER BRAND ATC PLUS Forbrennir.gsprodukter ved brann: Prosedyrer ved slukking av brann: ji 1 6- - -T-I-L--T---A-X-----V--E--D-- -U- T- I-L-S-I-K-T--ET- --U T_ _S_L_I_? ?_._ _ _ _ -- ! --1 Personlige vernetiltak: Se vernetiltak under andre punkter i dette HMS-databladet. Tiltak ved utilsiktet utslipp: Ventiler omrcet. Spill avgrenses umiddelbart- Dekk til med et absorberende materiale. Spill samles oop umiddelbart. Rengjar omrdet med var.n. Piasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfail: Se punkt 13, Fjernir.g av k jemikalieavf all. 7___H_A_N_D_T_E_R_I_N_G___O_G__0_P_P_3_Z__V_A_R_I_S_G_._______________________________ .________________ j 1 Batir.Mgkeiiskerke for sikker cppbevaring: lagres i orarader hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader Celsius. Lagres ved 'temperaturer over 0 grader Celsius. Hold behoiceren tett iukket nr produktet ikke er i bruk. j- Uforenelige materialer: Ventilasjon: * Oppbevar embaliasjen pi et godt ventilert sted. Sikkerhetstiltak for i forhindre brann: Sikkerhetstiltak for k forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for i forhindre statisk elektrisitet: j !I ! Forholdsregler ved bruk: Hold embaliasjen tett Iukket. Rayking forbudt: Under bruk av produktet kan roykir.g forirsake forurensning av tobakken og/eiler rayken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadalige dekomponenter. L99ab3.1r2e-f1.no.: ^ g e 24 of31 FC-602 LIGHT WATER BRAND ATC PLUS II 3 e x s ?o n s r :::g s x o n t r c i i c g p e r s o m l i g v e r n e u t s t y r . I 1 i Qveverr. : Unng fa damp, spray eller take i aynene. 3ruk tette vernebriller med ventiler. Hindverr.: 3ruk egnede vernehansker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av falger.de materiale(r) ancefales: Butylgummi. Annet hudvarn enn hir.dvern: U.nr.gi hudkontakt. Andedrettsvern: Unngi inninding av luftbiret materiale. Basert pa konsentrasjonen av luftforurensr.ir.ger i arbeidsatmosfaren veiges er. av falgende tvpe(r) godkjer.t ir.cadrettsvern: halvmaske med filter mot orgar.iske damper og vsskeformige partikler: A/P2SL. Svelging: Det mi ikke soises, drikkes eller raykes under arbeid med prccuktet. Vask utsatte omrider grundig med sipe og vann. Vask hendens etter hir.ctering og far spising. Anbefalt ventilasjon: Kvis utsugningsventilas jon ikke er tilstrekkelig, mi egnet godkjer.t indedrettsvern benyttes. Sarg for tilstrekkelig ventilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger: Ingen adm. norm. 1,2-ETAN'DIOL (107-21-1) 8-timers verdi ppm: - (stav) 8-timers verdi mg/m3: 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2-(2-BUTOKSYSTOKSY)-STANOL / (DIETYLENGLYKOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Inger, norsk adm. norm). FLUORALKYL OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. SYNTETISXE OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. 000257 L9a93b1.r2e-f1.no.: Page 25 of31 FC-602 LIGHT WATER BRAND ATC PLUS ----- rtwtr-ru-.:j u-.rAr-- (>rj jj j -u ;------------ Anmerkninger: Ingen adm. norm. TYKNERE (Trade Secret) Anmerkninger: Ingen adm. norm. RESTER AV ORGANISKE FLUORKJEMIKALIER (Trade Secret) 8-rimers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. 9 FYS15KE OG KJEMISKE SGENSKAPER. Tilszandsform, farge, lukt: Ugjennomsiktig, ravfarvet, viskos vaske. pH: ca. 8.5 Kokepunkt/-intervall: ca. 93.00 C (3egynnende) Smeitepunkt/-intervail: Ikke relevant Flamaepunkt: Selvantennelighet: Ingen (Setaflash CC) Ikke relevant Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, ovre: Ikke relevant Damptrvkk: ca. 16 mmHg (Kalkulert v/20 C) Loseiighet i vann: 3landbar. Spesifikk vekt: ca. 1.06 (vann=l) Damprezchet: ca. 0.85 (lufz=l) (Kalkulert v/20 C) Flyktige organiske forbindelser (VOC) " 339 g/1 SCAQMD (Kalkulert v/20 C) Fordampningshastighet: < 1 (butylacetatl) Viskositet: Ikke kjent 10 STA3ILITET OG REAKTIVITST. L9a9b3.1r2e-f1.no.: Page 26 of31 FC-602 LIGHT WATER BRAND ATC PLUS I Forhoic som skai unngis : I Mazerialer som ska unngis: Ikke kjer.z. Farlige spaltningsprodukter: Xarbonnonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Termisk dekomponerir.g av bruksferdig bla.nding forvenzes ikke representere noer. risiko. Stabilirei og reakzivitet: Stabili. Farlig polymerisasjon vii ikke forekomme. 11 0??!Y5jtigER OM KELSEFARE. Effekter eyr.e: Mild oveirritasjon: syraptomer kan vare rcdhez, hevelse, smerter og trsr Effekter huc: Mild hucirrizasjon (etzer langvarig eller gjentatt kontakt): svmptomer kar. vare radhet, hevelse og kloe. Sffekter innnding: Er. enkelt overekspor.ering som overstiger ar.befalte administrative normer, kan fare til: Pvirkning av sent'ralnervesystemet : symptomer kan vare hodepine, arhet, sovnighet, manglende koordinasjonsevne, nedsatt reaksjor.sevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon {avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slevhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Svelging kan fere til: .Pvirkning av nyrene: symptomer kan vare redusert urinmengde, blod i urinen og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er )0 0 2 5 tA iMO.rej.no.: 99312-1 rage / oj il FC-602 LIGHT WATER BRAND ATC PLUS oppkast.): svmptomer kan vare hosting, pustevansker, gisping, hoste opp blod og lungebetennelse sora kan vare livstruende. Informasjon on allergi, overfolsomhet : * Kreftfremkallende egenskaper: Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dette produktet inneholder en eller flere organiske fiuorkjemikalier, sotn muligens kan absorberes og lagres i kroppen over le.ngre tic, er.ten som utgangsmolekylet eller som r.edbrytningsstof fer (metaboiitter), og kan akkumuleres ved gje.ntatte ekspor.eringer. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nir de brukes som forutsatt og i henhold til veiledning. Forekomste.n av organiske fIuorkjemikaiier i blodet til den generelle befclkningen og underpopulasjoner som arbeidere, har | vart p.ublisert siden 1970 irene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. Et resym5 vedrarende produktets giftighet er tilgjer.gelig pa foresporsel. 12 QPPLYSNINGER OM MILJ0FARE. Persistens/3iologlsk nedbrytbarhet: Dette produktet inneholder en eller flere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljaet (persistente). Okotoksikologiske data: Et 3M milja-produktdatablad er tilgjengelig oh foresporsel. 'Andre skadevirkninger, milja: Nedbrytbarhet: COD (Chemical Oxygen Demand): 0,552 g/g; BOD 5 dagers (Biochemical Oxygen Demand): 0,254 g/g; BOD 20 dagers: 0,422 g/g; 20 dagers BOD/COD: 0,76. Akvatisk giftighet: 96 timers LC50 for fisk (Fathead minnow (Pimeohales oromelas)): > ) 0026l L9a9b31.r2e-f1.no.: Page 28 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 1000 mg/1; iB timers EC50 for daphnia (Daphnia magna): 1000 mg/I; S minutzers og IS minutters EC50, Photobacterium phosphoreum (Microzox System): henholdsvis 210 mg/1 og 220 mg/I. * Sorg for avfallshir.dcering ihz. gjeldende forskrifter. 13 FJERNING AV KJEMIKALIEAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mcttaks- eller behar.dlingsanlegg for.'spesialavfall. Ar.befalte avfallsgrupper: HOVEDKATEGORI 07: AVFALL FRA ORGANISEE PROSSSSER. . UNDSRXATEGORI 07 06: Avfall fra P3DB av fsttstoffer, sape, rengjoringsmidler, desinfeksjonsmidler og kosmetikk. EAX-koce 07 06 03: Halogenerte organiske losemidler, vasksvasker og mocerlut. Avfailszoffnummer: 7151 Organisk avfall med halogen. Spesielle komponer.zer: Etylenglykol, flzorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-kiasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: IMDG-side: EMS-nr.: MFAG: 3 L99a3b1.r2e-f1.no.: Page 29 of31 FC-602 LIGHT WATER BRAND ATC PLUS Ovrige opplysninger: Ikke transportfarlig gods. 15 OPPLYSNINGER o h Lo v e r OG F0RSKRIFT5R Merkeetikett: Symbol(er): KELSESKADELIG (Xn) Ir.r.eholder: Etyler.glykol 13-22 % 2-(3utoksyetoksy)-etanol 10-15 % R-setnir.ger: Farlig ved svelging. Kan irrigere aynene, huden og luftveiene. S-satr.inger: Ur.ngi i.nninding av camp/spravtetake. Ur.ngi kontakt med aynene og iar.gvarig kontakt med huden. Mi bare anvendes pi godt ventilerte steder. Fir man Stoff et i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask med sipe og vann. Ved svelging, kontakt lege omgiende og vis denne beholderen eller etiketten. Oppbevares utilgjengelig for barn. '/--gruppe: 00 Korr, yrkeshyg. luftbehov: 0-30 m3/l Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert pa tidligere merkeforskrifter og vil vare i finne pi produktet er. tid fremover. Merkingen vil imidlertid e'rstattes av folgende merking, som er basert pi rsviderte merkeforskrifter av januar 1993. I Symbol: Ingen Ir.neholder: 1,2-etandiol, 2-(2-butoksyetoksy)-etanol Kan irritere aynene, huden og luftveiene. Mi bare anvendes pi godt ventilerte steder. Unngi inninding av damp/spraytetike. Unngi kontakt med aynene og langvarig kontakt med huden. Fir man Stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask med sipe og vann. Mi ikke' svelges. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. Oppbevares utilgjengelig for barn. YL-gruppe: 00 00026 L9a9b3.1r2e-f1.no.: Page 30 of 31 FC-602 LIGHT WATER BRAND ATC PLUS Spesielle lover og bestenneiser: 1. Forskrift or: utarbeidelse og distribusjor. av Helse-, Mil jo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om kiassifisering og merking av farlige kjecikalier. 3. Forskrift ora kiassifisering, merking m.v. av stoff son kan medfore miljofare. 4. Forskrift on stoffkartotek. 5. Forskrift ora spesialavfall. 6. Etc. I ! ; i i I I I ! 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUKERNES SIKKERHET OG HELSE. Anvendelsesomrder: Filradannende skurakonsentrat for slukking av brann i hydrokarboner og Dolre losenidler eller vsker. Viktigste kilde(r) ved utarbeideisen av HMS-databladet: Databad fra 3M General Offices, St. Paul, Minnesota, USA. rsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og KMS-databladforskrifter,fastsatt i jar.uar 1993 . Etc. Egr.e opplys tinger : Informasjonen p dette `HMS-databladet er i henhold til vare nvsrende opplysninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medfarer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. j i :I | j i ; ! | ! II 00063 L9a9b31.r2e-f1.no.: Page 31 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS FOLGENDE PRODUKTES DEKXES AV DETTE HMS-DATA3LADET : 3M ID-.-.r. : XF-0008-0221-5 3M AFFF/ATC FC-602 Brannsluknin gsskum Produksjon 3M ID-r.r. : XF-0008-0100-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602,1000 L 3M ID-nr. : XF-0008-0078-9 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602, 20 L 3M ID-r.r. : XF-0008-0077-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602, 200 L I I ! i I I ii IIi s ooo 264