Document B5KJK0V2Y7Xr1nba3MV2dV9Jo

Wildlife International, Ltd. a. Pues Number 454A-105 PFOS: A 96-HOUR STATIC ACUTE TOXICITY TEST `WITH THE FRESHWATER MUSSEL (Unio complamatus) ADDENDUM WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-105 3M LAB REQUEST NO. U2723 SeriUeS8.50En-viErcolnomgeinetaallEPfroftcesctTioontAgueindcdyines OPPTS Numbes 830.1075 and `OECD Guideline 203 ch`Tahriascsitrutdaytwiaosnrweapsorrteevdiusseidnogna5t1e7st0s0uabsdtanthceeppuurriittyyowfas90d.e4t9er%m.inTeheftebset s8u6b9st%a.ncTehe Sponsor requested that the results be revised basonethde mostrecent purity analyses. The results fpRrrooevmaiotoeusmrlpytgpvrraeasseunmatdaeaddlteaw.teorAersesciSlmuocpuhl,syticeonrarnneaclteyetfdiocbaryl sdtuthaen1rdaartriooduoncfdotnihcneegnttmrwaaotyipbounreistpoirreesmsoeerantsa.ufraAectdtoacrcoonhcdfenst0.rh9a6t0e3i.ons revised summarypagefromthe final reapndtohermot strecentcertifofiacnaalytsies. g ay Ss 32 o 87 Wildlife International, Ltd. 2. Project Number 4544-105 REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% `SSPPOONNSSOORR:'S REPRESENTATIVE: LocATIONOF STUDY, ja DFArTiaA.ARNEDPOARCTOPYOFTHE EUTD, TPROTTEECTRNNAUMTBIEORN:AL Test SUBSTANCE: 3MsM, SCuorspaornatAi.onB:asch `WEialsdtloinf,e IMnatreyrinaatnidon2al16L0td1. asaanios PFOS (PerfuSlofrniocAocidcPoitasnsieum Sal) STUDY: MEAN MEASURED TEST `CONCENTRATIONS: PFFrOSe:erAMu9s6s-eHloUurniSotactoicmpAlcaumtaersT)oxicity Test with the Negative Control, 5.1, 12, 19, 39and 76mga.i/L "TESTDATES: Levomi OFTEST "TEST ORGANISM: SOURCE OF TEST ORGANISMS: AGEOFTEST ORGANINS MREEFARSEUSREENMTEATNITVSEOMFU2S0ELS TwOeTrAwLEGNHOTTH0)(um): SERRE, 95% CONFIDENCELIMITS: NOMORTAUTY CONCENTIATION. `ExperSitamrte-nMatya28l, 1999 Biological Termination -- June 1, 1999 `Experimental Termination ~ June 3, 1999 96 Hous Freshwater Mussel (Unio complamaris) Carolina Biological Supply Company Burlington, North Carolina 27215 Unknown MMeonn=z7i6t57, KaRnagnogoe=4587i501039e35 Trai Hadesmgail 19 mga iL Wildlife International, Ltd. 3 CERTIFOIF ACNAALYTSIES Project Number 4544-105 INTERIM CERTIFICATE OF ANALYSIS Revision 19/7/00) Centre Analytical Laboratories COA Reference #: 023-018A 3MPRreodfuecrte:nc#Pe:FOSSD,-L0o1t8 217 Purity: 86.9% ee OE FT, entificaion Re ee] Metal1.s ICalcium f a omiale sess 32.. MSoadginuemsium 4. Potassium S Lhaimen s Es fT SE 23 o Lawveen 4 6849wim % 5. Nickel Fu ah SUE 5. <0.001 wt/wt% 67.. Iron Manganes Limam 0 | m 6 000e s wmn% [Total% Impurity NMR)| TnmT r T osm | (LC/MS) Ea = (TGoCt/alMS%)Impurity RelatedCompounds = = [= TaTnT : | None Detected [Residual Solveats (TGA) [~~ ="= Ll TT NoneDetected | aT =n] No Applicable" | InorganiCchlorAinidoens IC) [1b 5 ITIiNRT hEan TasiTT e 1001S ws 2. Fluoride 3. Bromide hi sein gd hii | aE i 2 osowian 3. <0.040wt/wt % 45c. hNNiitrutiteem 7._Suifae! f[E iEiE a Le || sl <S o000wse wisnn | Seen OguLicTAFaAs TIO FlEELs CL orem 2 PRA 43. NHFFPBAA P Hie. mi]| 325 o o ww w ee e e Elemental Analysis" 21.. CHyadrrboognen 43. SNuiltfruorgen 5. Fluorine 21 `TThheeoorreettiiccaallVVaalluuee ==01%7.8% 43.. `TThheeoorreetticiaclalVValaue=l=50.u%95e% 3. Theoretical Val =6u0e% 21. 01224448 wwt//wwt%% 43 817B4wwiAnns% 5. 54.1 wwt% coan18A Page lof CeIntNreTAEnaRlyItiMcalCLEabRoTraItoFriIesCCAOTAEROefFereAncNeAf:LY02S3-I0S184 DateofLast Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01 F"lPuuorriitdye=, 01.0509%% -N(MsuRm oifmmpeurtiatlieism,pu1r.it9i3e%s,or1g4a5ni%c+aLciCd/iMmpSuriimtpiuersi,ti0e.s,388.%4+1P%O+AInAo,rganic 0.33%) Total impurity from all tests = 13.09% Purity = 100% - 13.09% = 86.9% ?Potassium is expected in this salt form and is therefore not considered an impurity. o*bPusreirtvyebdyfDorStChisissgaemnpelrea.lly not applicable to materialsof low purity. No endotherm was "iSnuolrfguarniinctahneiosnammepltehoadppceoanrdsittioonbse. co`TnhveeratneidontoreSsuOl,t aagnrdeehsenwceelldweittechttehdeussuilnfgurthe odentetrhemirneastulitosn, itnhethSe Oeleimsennottalcoannsaliydseirse,dalenndiimnpgurciotnyf.idence to this interpretation. Based TFA HFBA NFPA PFPA `Trifluoroacetic acid Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid "Theoretical value calculations based on the empirical formula,CeF17SOsK'(MW=538) "This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160). COAO23018A Page20r3 INTERIM CERTIFICATE OF ANALYSIS Centre Analytical Laboratories COA Reference #: 023-0184 LC/MS Purity Profile: CC impurity 7 weme% | eas Ncoutrev:es,TrheespeCc4tiavenldy.CT6hv e Ca S wvl ealrueeu cwaalcseuclaals tceudluastiendgutsihnegCt4heaanvdeCra6gestraensdpaorndscealfiabcrtaotrison from theC4and C6standardcurves. Likewise, theC7valuewascalculusaintgethde average responsefactorsfromthe C6 and C8standard curves. Prepared By: Davids. Bell Date Reviewed By. Scientist, Centre Analytical Laboratories John Flaherty Date Laboratory Manager, Centre Analytical Laboratories coanz3-0184 Page 3of3 ilCOdTlOiXfICeOLIOGnYt&eArNAnLYaTtICiALoTnEaSTlIN,G SLERtVIdCE.S September 27,2000 3MsM. CSourspaonraAt.ioBneach 39En3Mv5iBrBuouinslmhdeinAntvgae2ln-Lu3ae6b-o0r9atory P.O. Box 33331 St. Paul, Minnesota 55133 Dear Susan: 454 - 10 Enclosed plase 2, 454A-103A, find Addendums for Wildife Intemational, Lid. 54A-104, 454A-105, 454A-106, 454A-107, 45 4 Projct Numbers 4S4A101, A-108and 454A-109 Ifyou have any questions, please let me know. Sincerely, : EKrioy. a KurtR Drottar `Senior Biologist E85e98nCoOmmeercOerDraiveeeoEsrton1,AM-ar2yaAndr2401iTTeleo4N0arb-a2r2i-O 86T0o0T Fx 41S 0.822T .06ro72-HEOSm66ec2o8toxEGuriadifpeinoernntidomnmsoicnonmt |