Document 9o3RGNZgbrn6ov0OZMDOBZx5

Monsanto r * OM LOCAI (OS DAT t 1UBJCCT Ashley Peter G3WD 4-8831 January 22, 1985 cc Dr. George Roush - G2WG Dr. W.J.McCarville -G3WD Toxicity of Chlorinated Dibenzodioxins ncrrflCNCt TO J. D. Wilson - G3WD I have enclosed the relevant pages of two articles dealing with the toxicity of certain chlorinated dibenzodioxins. I have also enclosed a table constructed by myself from a number of references dealing with acute toxicity and some other effects of chlorinated dibenzodioxins. I hope you will find the information to be useful. /vs Attachments SU3J i Acute Toxicity nnd Other Effects of Various Chlorinated Dibenzoi-p-dioxinn Acute Toxicity - Oral LD50 Induction of AHH Activity Guinea _2is Rat Mouse PCDD (nmolcs/kg) 1 HsZiisi2 HB/L82 (R S Z liS l2 .2,3,4 ,2,6,7 ,3,6,8, ,3,7,9 ,3,7,8 2,3,7,8 ,2,3,7 ,2,3,8 .2,6,9 ,2,6,7 ,4,7,8 ,2,4,7 ,2,4,8 Jmixed>100,000 6 0.6,2.1,2 22(H),45(F) 280 i ED50 (nmoles/kg): Rat Hepatqma Chick Liver3 Cell Line4 Approx. Activ. Binding Aff. Relative Kd (nM) to TCDD Chick Mouse Liver Embryo Assay-6^' Cytosol6 Inactive 0.002 Inactive Biologi < Response (pg/_mi )` 0.1 150 .35 .38 1.0 .27 50 150 150 5000 5000 5000 5000-15. 5000-15, ,2,3,4,7 ,2,3,7,8 ,2,4,7,8 9 3150 3 1100 360 >5,000 .70 5.5 >1.25xl05 0.1 0.3 .62 2.3 ,2,4,6,7,9 ,2,3,6,8,9 ,2,3,6,7,9 ,2,3,4,7,8 185 ,2,3,6,7,8 178-255 ,2,3,7,8,9 153-255 Lxed isomers 73 70-100 60-100 100,000 825 1250 >1660 - .88 .37 .66 7.8 32.0 67.3 0.002 0.15 0.3 0.25 0.25 .77 .57 .32 ,2,3,4,6,7,9 2,3,4,6,7,8 >1600 >600 5.6 138 0.002 0.15 2.1 :dd >1 ,000,000 >6,000,000 0.002 TO X' SUBJECT TO PROTECTIVE ORDER. REFERENCES 1. McConnell, "The Comparative Toxicity of Chlorinated Dibenzo-p-Dioxins in Mice and Guinea Pigs", Toxicol. Appl. Pharmacology; Vol .44, ISS2, 1978, pp. 335-336. 2. K. Payne, J. Chem. Ind. (London), 1982, pp 298-300. 3. J. Knutson, A. Poland, private communication, J. Knutson (In Press). 4. Bradlaw and Casterline, "Induction of Enzyme Activity in Cell Culture: A Rapid Screen for Detection of Planar Polychlorinated Organic Compounds", J. Assoc. Off. Anal. Chem, Vol. 62, ISS4, 1979, p p . 904-916. 5. Activity data provided by A. Poland, found in "Report of the Ad Hoc Study Group on Pentachlorophenol Contaminants", Environmental Health Advisory Committee, Science Advisory Board, U.S. EPA, Dec. 29, 1978. 6. Poland and Glover, "Stereospecific, High Affinity Binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin by Hepatic Cytosol", The Journal of Biological Chemistry, Vol. 251, No. 16, Issue of August 25, 1976, pp. 4936-4946. 7- J. Knutson, A. Poland, private communication, J. Knutson (In Press) SUBJECT T *J i i