Document 9nqygZa7OLwrko8pE543EpZ6
AR&26 -- 0728
MICROBICS MICROTOX TOXICITY TEST
IESTSUBSTA0N 000C 000E Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfpoonanteen,twohficFhC-m2a0y3.al(s1o- be
Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-203 without tolyltriazole (VW160390). Current information indicates it is
a20m%ixteutrheyloefne1.g3ly4c%olP, F2O.6S6, %35S%uldtioentehyfloeanmeerg,ly3co%l bsuotdyliuemthoecrt,yl37su.l9f0at%e,waatnedr, 0.1% sodium lauryl sulfate.
cThhaerafcotlelroiwziendg scounmcmenatrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample.
ME0 MHOD 00000 Method: Microbics Microtox "BASIC" Procedure
Test type: Acute static GLP: No
YSepeacriCeos:mplPehtoteodb:ac1t9e9r1ium phosphoreum Analytical monitoring: pH and osmotic condition. SEtxaptiosstuicrael pmeertihodo:ds3:0-EmCin5u0tevsalues calculated by statistical linear regression program provided for Microtox
Test organism source: Microbics' Reagent.
Test coDnidliuteinto:nsM:illipore Mill-QTM water. Test temperature: Not noted.
Stock and test solution preparation: A primary 2000 mg/L stock solution
was prepared by dissolving 200 mg in 100 mL Millipore Milli-Q" water. The ~
PH of the stock solution was adjusted from 5.7 to 6.6 using 1.0 N NaOH. An osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL Cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
Results
0000000
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
000657
Element value:
5-minute ECso = 1000 mg/L. 15-minute ECso = 550 (520 - 590) mg/L 30-minute ECso = 320 (300 - 350) mg/L.
Element values based on nominal concentrations.
SRuebmsatraknsc:eTReesmtianrgkwsafsielcdo. nTduhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiabtemdixitnutrheeaTnedstnot the fluorochemical proportion alone.
Conclusions
000
dTehteertemsitnseudbtsotbaenc3e2300-mmgi/nLutweitEhCas,95fo%r Pchoontfiodbeanccteeriinutemrvpahloosfp3h0o0retoum35w0as
mg/L.
=
`Submitter: 3M Company, Paul, MN, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
bataQuaury
0000
Reliability: Kiimisch ranking 2. However, sample purity was not
Testing properly
meets all criteria for quality testing. characterized and the study lacks
analytical solution.
confirmation
of
the
amount
of
fluorochemical
proportion
in
the
REFERENCES
000
This Lab
study was conducted by the 3M Request number J1608-8 1991.
Environmental
Laboratory,
St.
Paul,
MN,
LL
Last changed: 6/27/00
000658
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TEST SUBSTANCE: 0-203 withadt Tolptrizole (Vis 10395)
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micRoTOX rs DATA EET
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HICROTOX(r) DATA SHEET
iii
FE-203 w/o Tolytriazole (Wi 160390) 1S-min exo. J1608-8 7/19/91 sab
i
PAIR
conc.
T2TT 1m25.a000m
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R%0.99738 SLOPE = 1.0181 INTERCEPT = +e.ti27
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4ICROTOX Cr) DATA SHEET FG-203 W/O tolytriasole (VW 160390) So-min exo. J1605-8 7/19/91 sab
.AR 8 conc.
1o0/t1e
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C50 = 320.207 (303.627 10 346.353 )
(1+ 6cbs)
ECs,= 330 (3c0- 350) malt
R "0.9942 SLOPE = 0.9279 INTERCEPT = +5.7816
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