Document 9LyrV87qREK3Gpg3RenxxDo1D

MICROTOX(D TOXICITY TEST TEST SUBSTANCE Identity:Perfluorooctylsulfonadtied,ecyldimethylammonium salt;may also be referredtoas Fluoroalkyalmmonium derivative[.1Decaminium, N-decyl-N,N-dimethyl-s,altwith 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-loctanesulfoniaccid (1:1)C,AS # 251099-16-8] Remarks: The 3M productionlotnumber was Lot 1. The testsample is L-14394 referredto by thetestlaboratoryas P3025. The sample was labeledF-11615, Lot 1. The testsample isa mixtureofthe testsubstance inwater (approximatel3y0-40% testsubstance,60-70% water,and 0-5% of residualperfluorochemicals)A.llvalues reportedrelatetothismixture. The testsample appears tobe a 2-phase dispersion(clearliquidwith opaque solid)which rapidlyseparatesafteragitationN.o calculations were made to adjustforthe actualconcentrationofthe testsubstance in the testsample. METHOD: Method: Azur EnvironmentalCorp. Microtox@ "BASIC" Procedure Test type:Staticacute GLP: No Year study completed: 1996 Year reportcompleted: 1997 Species: VibrifoischerfiormerlyPhotobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition Replicates:Two Statisticamlethods: EC50 valuescalculatebdy statisticlailnear regressionprogram providedforMicrotox@) Test organism source: Azur Microtox@)Reagent. Test Conditions: Dilutionwater: Azur EnvironmentalCorp. diluent Stock and testsolutionpreparation: Water accommodated fractionsT.est solutionswere prepared foreach testconcentration by mass additionoftestsubstance inMilliporMeilli-Q"wmater. The solutionswere stirreodvernightinthe dark atambient room temperature.A whiteprecipitatweas observed inthe stock solutionsaftermixing.Priortotestinitiatioan1,0 mL aliquotofthe clearliquidportionofeach testsolutionwas removed and each was osmoticallaydjusted. Alltestsolutionswere made by proportional dilution. Exposure vessels: 4 mL glasscuvettes. Number ofreplicatesT:wo Number ofconcentrationsF:ourplusa control. ElementBasis:Percentlighltoss. RESULTS Nominal concentrations: Blank control1,25, 250, 500, 1000 mg/L Element value: 5-minuteEC50 = 600 (580-620)mg/L 15-minuteEC50 = 510 (500-530)mg/L 30-minute EC5o = 350 (240-490)mg/L Allelement values based on nominal concentrations. Remarks: Values reportedare forthe testsample. No calculationwsere made toadjustforthe concentrationofthetestsubstance inthe test sample. CONCLUSIONS The testsample 30-minute EC5o forVibriofischerwias determined to be 350 mg/L witha 95% Confidence Intervaolf 240-490 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations inthe testsolutionsand sample purityisnot sufficientclhyaracterized. Additionallyd,ata isfora mixtureand toxicitcyannot be positively attributetdodidecyldimethylammonium Perfluorooctylsulfonastaeltalone. REFERENCES This studywas conducted by the 3M Company EnvironmentalLaboratory, St Paul,MN, Lab Request number P3025. OTHER Last changed: 5/24/00 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON MICROTOX@ Azur EnvironmentalMicrotoa System STUDY COMPLETED: December 19,1996 FINAL REPORT COMPLETED: May 12,1997 Env. Lab Request Number P3025 Prepared by: Name: Title: Laboratory: Address: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory Building2-3E-09 935 Bush Avenue St Paul,MN 55144 Microtox,g Page 1 May 12,1997 SUMMARY The purpose ofthisstudywas toevaluatetheinhibitoerfyfectof P3025 on bioluminescentbacteriainaccordance withthe Azur EnvironmentalCorp.Microtox(tDest.The median effectivceoncentration3,0-minuteEC50 was reportedas 350 mg/L. INTRODUCTION ToxicittyestswithMicroto& providea rapidscreeningmethod and can be reliablperedictorosftheinhibitory effectsofcontaminantson microbiaslystems. Thistoxicitsytudy measured the lighotutputofbioluminescentbacteriaovera relativeslhyortperiod(5,15,and 30-minutes)inthe presence ofdifferenctoncentrationosf P3025. Test Substance: Appearance Aqueous Solubility AvailablePhysicochemical Properties: Date Received Storage Test Solutions Reported values MATERIALS ND METHODS P3025, date 10/7/96,Lot 1 Two-phase dispersio(nclearliquiwdithopaque solid) Apparentlynotcompletelysolubleinwater attestedconcentrations. BoilingPoint: Vapor Pressure: Vapor Density: EvaporationRate: Solubilitiynwater: SpecificGravity: PercentVolatile: pH: 1 OOOC ca. 18 mmHg @ 200C <1 <1 Insoluble ca. 1.2 60-70% 6-8 10/16/96 Inthedarkatambientroom temperature(22 20C) Test solutionwsere preparedas Water-Accommodated Fractions(WAFs). Four stocksolutionosf 250,500, 1000,and 2000 mg/L were preparedinMilliporMeilli0 water. Each was capped and allowedtomix overnighti,nthedark,atambient room temperature.Test substancewas apparentlynotcompletelymisciblewith water. A few whiteparticulatewsere observed ineach stocksolutioanftermixing. Justpriortotestinitiatiaon1,0 mL aliquotoftheclearliquipdortionofeach stocksolutiownas removed and each was osmoticallaydjustedwith200 mg reagentgrade NaCi. Each initicaolncentratiownas dilutedby 50% duringthe course ofthetest. The reportedvaluesare based on nominalconcentrationisnthe testmedia atthe beginningofthe microbiaalssay. Microtox(D Page 2 May 12,1997 Controls: Blank control Exposure: Test Date Contact Time Vessels Number ofReplicates Test SolutionVol. StartingTest Conc. Test Substance DilutioWnater Temperature Lighting Two controlscontainingMicrotox(5reagent (Photobacteriumphosphoreum), but no testsubstance. 12/19/97 5, 15, and 30-minutes 4 mL Azur Environmentalglasscuvettes Two 1.0 mL 125, 250, 500, and 1000 mg/L Azur EnvironmentalDiluent(uftrpaure waterosmoticallyadjustedto2% NaCl Instrumenttemperature set at 150C Ambient laboratorylight Measurements Procedure Lightreadingswere taken upon reconstitutionfthe Microtox@ bioluminescentbacteria, and then 5,15,and 30-minutesaftertestsubstance was added tothe bacteria.All readingswere captured electronicalvliyacomputer interfacweiththeMicrotoxS instrument. The testwas conducted ingeneralaccordance withtheAzur EnvironmentalMicrotox@ Basic Test. CALCULATIONS The reportedvalues are based on nominal concentrationsinthe testmedia atthebeginningofthetest,The concentrationstestedand the correspondingresponse data derivedfrom the toxicittyestare used tocalculate,it possible,the medial effectiveconcentrationE,C50 and the 95% confidenceintervably standard logistic regressionmethods provided inthe Azur Environmentalsoftware.The EC50 isdefinedas the concentration estimatedtoinhibitthe rateof bioluminescenceby 50% as compared tothe controlsa,fter5,15, and 30-minutes of exposure tothe testsubstance. Microtox,S Page 3 May 12, 1997 RESULTS The percentreductioninlightoutputand the median effectivceoncentrationE,C50, obtainedafter5,15 and 30minutes exposure tothe testsubstance P3025 are summarized inTable 1. The median effectivceoncentration3,0-minute EC50 and 95% confidenceintervawlas reportedas 350 (240-490) mg/L. ACCEPTABILITY QRITERIA Thistoxicittyestmeets qualitycriteriparovidedby Azur EnvironmentalCorporation: 1) The 95% confidencefactorforthe EC50 was <1.3 forthe 5 and 15-min exposure periods. Itwas > 1.3at30-min,,however,thiswas probablydue tocomplete inhibitioantthe highest concentratiotnested(1000 mg/L) . 2) The coefficienotfdeterminationwas > 0.9 forthe 5 and 15-min exposure periods. Itwas < 0.9 at30-min;however, thiswas probablydue tocomplete inhibitioantthe highest concentrationtested(1000 mg/L) 3) The correctiofnactorwas > 0.7at5-min and > 0.6at15 and 30-min. This new versionofAzur EnvironmentalCorporationsoftwaredoes notprovidethe correlatiocnoefficient. Thistestwas conducted by Trudy K. Stiefenhoeferas requested by Pam Wolf of3M SpecialtyChemicals Division. REFERENCES 1. MicrobicsCorporation.1992. Microtox@Manual. Volume 2. DetailedProtocols.Carlsbad,CA. 2. MicrobicsCorporation.1993. MicrobicsCustomer TrainingManual. From February,1993 workshop. Microtox@ Page 4 May 12,1997 TABLE 1 SUMMARY OF TOXICITY OF P3025 TO MICROTOX PERCENT TEST CONC. mg/L 5-Min. Exposure 125 14 250 15 500 26 1000 95 EC50 95% Confidence Interval 600 (580-620) Statisticamlethod used Mean of two replicates Azursottware LIGHT LOSS' 15-Min. Exposure 22 25 47 98 510 (500-530) A2ur soirware 30-Min. Exposure 28 33 62 100 350 (240-490) A2ur software