Document 9L3vap5y0426y4G2xy6jRjEK5
MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to `as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: 3M production lot number 380. The test sample is FC-126, a white powdery solid. It's purity was not sufficiently characterized, though current information indicates itis a mixture of 78 - 93% test substance and 7 - 22% Cs, Ce, and Cy perfiuoro analogue compounds. METHOD: Method: Beckman's Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1987 Species: Photobacterium phosphoreum Analytical monitoring: Light loss. Exposure period: 30-minutes Statistical methods: Not noted. Test organism source: Not noted. Test conditions: Diluent: 2% NaCl Microbic's Reagent Deionized water pH: 6.5 Stock and test solution preparation: A 2000 mg/L stock solution was prepared by dissolving 200 mg of the test solution in 100 mL diluent. Test solutions were prepared using aliquots of the stack solution. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: Cuvettes. Number of replicates: two Numberof concentrations: Four plus a blank control RESULTS Nominal concentrations: Blank control, 420, 560, 750, and 1000 mg/L Element value: S-minute ECz0=810 mg/L minute ECs = >1000 mg/L S-minute ECgo = >1000 mg/L 15-minute ECz0 = 520 mg/L 15-minute ECso = >1000 mg/L 15-minute ECso = >1000 mg/L
CO3861
30-minute ECz = 420 mg/L
30-minute 30-minute
ECs ECap
==
>871000(081m0g-/9L30)
mg/L
All element values based on nominal concentrations.
CONCLUSIONS
The FC-126 30-minute ECs for Photobacterium phosphoreum was determined to be 870 mg/L with a 95% Confidence Interval of 810 to 930
mall.
`Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Rteesltiianbgi,libtuyt: lKalckismiasncahlyrtainckailncgon2.firTmeasttiionng omfeteetsts salulbcsrtiatenrciaefcoornqcueanltirtaytions. There is a lack of characterizationof the test sample. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number E1282. OTHER Last changed: 5/24/00
03862
Lo.
ENVIRONMENTAL LABORATORY
BECKMAN"S MICROTOX SYSTEM
HIGROTO RAV DATA
RE) 2: 3) 3
Test sosstance:_FC-J2L Lod 390
TEST DATE: ~29-8
ARALYST:
.
SAMPLE CUVETTE
Bn a
2
2
3
c
2 ch
5
[4
Te(swihiStuebsptoawndceer) 2
|
Dilvent 3
TeiN nt Di+ ets
DL wake Used!
FINAL ASSAY CONmCgENTRAJTLION BLANK CONTROL DUPLICATE
R1E0A)DING |156-;MIN,
40 9 28 36
TERBIRGS T1E 05)E |+ 103. 0)
89 97 se 85
Yao
Duplicate
q3
23 7
69
qq 90 | sa 74
S60
Duplicate
as
23
74
te
lea 90 79
71
760 Duplicate
lot 23 bq 58 a8 go |u7 Sg
loco
Duplicate
as | ee
| |70
uq 3g
|49 | 3%
Steck. Seln. Appeared +o
coms fiomL I=m am
Sool completely
Inal phiz 5.7 Adyusted +o 6,7 wih dilote Nach
A % Lack Micrebics Reagent
Tot)Coe SO ATSL wee "
5
he final assay
Introduced transferel
indo the top (50/50) into
Tow (Rew the muddle
4) and were then and the lower
tows (Rews B+C).
03863
putiled 4-70-97 pH bes"
3
`
BLANK RaTiosk = FTionatl
BECRMN"Ss MIGROTORRststen
HICROTOX" DATA REDUCTION
FC-12L Let 390
Wate.R_E1J-229-&27R
AnalystSAR
8 Ais
titoon fafectt]oon *The blank ratios for Bl and Cl should agree within 0.02,
NE TLR RTO
BI BE RATIO + C1 BK RATIO
fetes] 0oo|
NORMALIZED Lica 10ss (Peace) Hees BankKatto fatcla Smple Resting
Boeo [omg Mest Concentration ERT
& 4ao "3 | oom
2750
E8Bs leoo
oo
[ones
"Y15
[oar rim .303 o.187
05..231043
L503
0.333
| oy Tees
ack
1 H1g
2 LIGAT Loss (24) = --1F8 --x 100
Er 8 seo |a fh E SMFLECWETTE | Sw.Ta | onw.Fa| Sewn.a |
2 yao MB Test Concentration
9a
7.5
7
Io
E2E3]
3
a1
gs
joo [T 21 r Tz
EFFECTIVE CONCENTRATIONS (eC7e)
Ce xe
som.
Tx,e 00 sio
Ee= 1790
bad
(ibbo=1346)
ECs= 3940
(Calculated using "%,C"
a pa
EC =- S20
Cg = 1100
(1050-1150)
ECgy= 2330
data pairs.)
[som|]
ao
870 (s10 930)
FY