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AttacShtmuednitess taondLeOtttehrertoICn.foArumeartidoanteodn MCearyta1i8n, 2000 AR2%6 31 Perfluorooctane Sulfonate-Related Compounds 11. y NEWFOSEMA Neethyl perfluorooctane |suifonamido ethyl methacrylate Acute Toxicity 1) AIacbuotreatOorrayl, RToixkiecriLtaybSocrarteoerniewsi,thIncT.,-3P4r9o4jeicntANlob.in0o88R4atAsR,0S0a1f3e,ty3EMvalRueafteiroennce No. T3494 (L-1048, FX-14), March 13, 1984 2) PlarbiomraartoyrSyk,iRnikIrerritLaatbioornaTteosrtiewsi,thInTc.-,3P4r9o4jeicntANlob.in0o88R4abEbBi0t0s1,2S,af3etMy REevfaelruaetnicoenNo. T3494 (L-1048, FX-14), February 7, 1984 3) lAacbuotreatOocruy,laRrikIerrritLaatbioornaTteosrtiewsi,thIncT.-,3P4r9o4jecint ANlob.in0o88R4aEbbBiOt0s1,1S,af3etMyREevfaelrueantcieonNo. T3494 (L-1048, FX-14), January 24, 1984 Immunotoxicity 1) 4G0u7i0n3e9a8P5i,g3MMaxiRmeifzearteinocne,NHoa.zTle-t3o6n10La(bNorBatNoor.ie6s36A0m1e-r3i2c,a,FXI-nc1.4,)P,roOjcetcotbNeor.9, 1984 Genotoxicity 1) ISnRIViItnrtoeMmaitciroonbailo,loPgriocjaelctMNuot.agLeSnCic-i3t1y45A,ss3aMysoReff3erMenCcoemNpoa.nTy-'3s6C1o0m(pNoBuNnod. T6-33661001, 32, FX-14), September, 1984 - ane 005510 Experiment Hout Conducted At: pates conducted: conducted BY: Reviewed BY: ues ons Torteiey seen "ith 7-3494 {a Atbino Tats {NweC n Jen " oasanso013 ScaetentayuelEr,vaatlMuoianrtniiecosnsot:LaaBnOeT:StOrY sanaary 19, 1984 to FRBEAEY 20 1998 DPeuxiiiconolnosRis,tmnsa5nnbboe5tr) Eface Touay Director oppSoarernazetonO eOdcroN,los5ie5O t H 3psledol e Soke. Toxicology or wY5TTb..2 cmGMaroesaaceenoesmiieenx 005511 1. summary i Lien 7-398 vas conducted 1% he acute oral 19, 1984 to toxicity FEBRRTY Seren 20 194 at Riker Laboratories: in BAY 32 st. Pauls eight from January sing male and femal aabino rate ranging BY GEStEiC intubation at J1i9n0n-e2s3o6tagrams. The testBa3X/XEIiCbIodaysweaidgmhitniwsittehrendo sorEaliEier noted during + dosage level of 5,000 ne 14 day observation periods pierrhea vas nhootveedveoxn, dvaoyulednebeaneaxpseucbtseidded by the day four cbaeTvaticn mia response, oil a8 tC enicte at a constant volume of 20 mL/kg. B03Y Necropsies sing cottonseed vere noted in 81 \ntsals at the end no of the SAY: Visible Lesions. The aight gains portorsed at termination of he study revealed than 5:00 ng/kg in fasted male approximate oral 1050 of r-3asa is greater ana female albino rats: Introduction of this SEY WS to determine the acute oral X05of the Food and, the objectivealbino TES mts study is not regulated of 1978, although Regulaticn rp-r3o4g94Maiinnifsatsrtaedtion's God LaboratoorfynPirsactLiacbeoratory adnere Eh general he standard operating procediet me raw data generated bY stay pDriirneccitpolresanodf tthheisfizneagluTleaRtoirotn A1 stored in the conducting Laboratory's archives. 005512 2. ong method and Results anime zats vere ued 17 restv,ithAoLnlaynisanailasslvserwehihcehldSwFetE quaransine fox soeed 878 FE and settanIe 2 eooeing oot antsate at the dnsetation OF 2 Ton aay tn goog weds hesith The sata vere hued 7 J a permitced a standard JOE orane aod mast controlled uring a 16-20 hous period ssmeaieeel J peter to uti treabation food vas vissheld at a single dosae Jew ne cate were administer6ed2e0 vS2a55nvevorltuanl of 20 S/H Tee 17 \11 doses re administered the Tat saing a hypodermic SYELRIS equipped With & he stomachs iiecippea of intpeeios needle of adstniasration 0 2% settote, tne mats vere Teed sen 8 eve susie thet cages and cheered 9 2 ortong ape 1 14 gaye. Tits and adverse rescsions (TLS wna cima body eights, BOLE animals that died during the - ty ao eh were recorded. A sn "as conducted on necropsy Shon svinenatize? = tre all and of the 14 day SPeSTEER in the study: period (Table 1)+ 208 `Theprotocols pErinHcipaalnpcerasnocnneels.taitnevmoelnvtedze contained 17 "posision ehassctasiscics appendices I-IV. 2cuasten sive Bo avorasarLteesn, T0ne: WThIensSBEeeTnet1er WO 3BSrinmi Paamat, hnco.sew Be FR 005513 oe --pose? lag/kg) so 5,000 TARGET ACUTE ORAL TOXICITY SCREEN ALBINO RATS sy: ith T-3494 MEFS Jenctions and Body Weight DAES sortality, Necrop BodyE weighE ts (9)T ymusbger mDea,d animal `pest Day 7 Number1:a umber Tested Sex Number MRA 22331a 228957 a3n3?9 288 o/s 4wrraat132 rare 22136 226683 285 330233 rats 236 207 251 226352 ors F 4ARRae3332 rade 119901 221264 241 22585 44rRaa33s6 210959 23 251 pE ercS ent Dead o test article Ws aninistered as & suspension in cottonseed oil: fasted pale and female Fats: 2 Tne sme acute oral L050 is Jeter nan 5,000 ng/kg in Levealed no visible lesions: Nreocrlopesys performed upon termination of the SBAY three, inclusive: advereseaRevaacsntiootnesd in all animate on days one thresh 005514 BESTCOPY AVAILARLF - meme appENDIX 1 - PROTOCOL rest. Acute Oral Toxicity aM CRIS om sis t. Paul,Minnesota spoNsOR: aluationLaboratory:Riker Laboratories, InC-+ S CONDUCTED By: SafetyEv -- TEST ARTICLE: -- in E Ht-- (CONTROLARTICLE: PROPOSED STARTIN GICOMPL ETION DATE OF TE ST : uf JSEOSUTRSCYES:TELM:HAFRrLeSwE eBweRrT,, o w a VETER, MPs. Division - : sNeuxm:beHr:F 5,5 Weight Range: 200-3% grams oe the tes veof is tost wil ba to charac 1%acute.aE m E alavailabilty. (OBJECTIVE: Tr heobjectiresponse, historicE al Use. "ease In handling! `and gener sh cageisn temperature and gd humid libitum : ducioifty of "The animal s will be hrionugsbeotdhitn hsetqauianlroealsnossrtticenoeedli`snaungds,tpaeecsnctdoerpdedirniwgoidr1es0.wmtiehtehe\faoeboodra?atonrydm'w'saatseetranodfafPredreR"do.pEeerlatdioneg sP METHOD EcEaoancctrhhoalalnnenidmera,olowmisll`cdbueoorntkeigfireGdi0b1bye2caZdmiLniistnegr,edserachsema,Tfhowei ver, if this de osedt lev Gasage of 2000 mo E Se S SimaE ls IntheformS receivede frdo`mbethhea.v`isopron i S e otocol. The e.testarticle willbreetuardnmeidn1i0sttehreeird 10 the cages an and observed for anyuntowar nis pr after which the `animals wil be r e E1E 8935 12, 5 theanimalrospssuyrvwiivlint ' cB S ondtupcertieodd.oi nA"nsayp.logcartnioicsmsamaelbr nsnwtoihroimnaclh1i0tdi5iceesewYhiech aarnedcooobrnsdeturcevteoodbfbodsr1ueafrtivonergdi.tehsAe' cC`oAnrLcdhuicv`etG,a`toSafttghe`enPaenurlea,ctMeidnnbeYsohtea. ttpodeisreectoriand the. final reportwillbe stored. in Stth.e LRoiukser,LaMissourt [a ity 8Bprunne LabwoaratteoryGCFhow,RaTlsHt0on0HP3Eur1iDn0a,2 wor ANH ga { DECE 3 Conon foo To Vk J 1al=l% Doin mers - 1s Date "Study Director "Sponsor. 0605515 5. ; Riker Experiment Noo ,_ossansooy = peviationas apnd/por1rA(pucenodnpacelnnutdsexd)2 rotocol 1. he weight range is extended E> 190-300 gn to expidite the Study: w DSarundy UDirmece atobror 2 aat/e84 2 Seudy Director Tate 3 Study Director Tate a Sealy Director ote 5 Seay Director ee 005516 6. -principal participatinAgPPpEeNrDsIoXnnIeTl Involved in the STUSY. punction name b. N. harkoe, Tres BS X. 1. Eobens, BS X. D. O'Malley, BS G. C. pecore rSotxuidcyolpoigziescttor su`Tpoexrivciolsoogry Testing sceuntieorTTooxxiiccoolloodgyist snuipneravlisLoarboratory 005517 7. APPENDIX IIT Composition Characteristics : This study is not regulated by the Good Laboratory Practice Act of 1978 and therefore information pertaining to composition characteristics is not applicable for inclusion in this study. 005518 5. APPENDIX IV guality Assurance Statement regultateionstoufdy19i7s6,noatndoftfhiecrieaflolrye TeqstualtaetmeedntB.ystihgemeGdooadndLpabroerpaatroerdybPyratchteice conpitance Audit department be standard operating is not spplicable: procedures of this laboratory does adhere to the general principles of this regulation. The compliance Audit for studies underisy in department the Acute does inspect different significant phases sociootogy Laboratory on & recurring cycle, and the factlities oxo examined on +"ttuhtrieoen maornethrosucthiendeullye.picIknedadadtitziaonndoan seXloemctthneumabrecrhiovfesReBsYeatrhcehc&omDpelviealnocpement Audit department for review. 005519