Document 9JZQLYoXa4vgzn8rBYm4MgKrR

MICROBICS MICROTOX TO XIC ITY TEST TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 502. The test sample is FC206CE. Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1 .55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD: __________________________________________________ M ethod: Microbics Microtox Procedure Test type: Static acute GLP: No Y ear Com pleted: 1989 Species: Photobacterium phosphoreum A nalytical m onitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical m ethods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Microbics 2% NaCI solution. T est tem perature: Not noted. Stock and test solution preparation: A primary stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was 6.7 and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "apparently miscible in water, no color apparenf. All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Num ber o f replicates: two Num ber o f concentrations: Four plus a blank control Elem ent basis: Percent light loss RESULTS___________________________ ;_____________________________ Nom inal concentrations: Blank control, 125, 250, 500, and 1000 mg/L 001749 Elem ent value: 5-minute EC50 = 650 (590 - 710) mg/L 15-minute EC50 - 450 (420 - 490) mg/L 30-minute EC50 = 420 (390 - 440) mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-206CE 30-minute EC50for Photobacterium phosphoreum was determined to be 420 mg/L with a 95% confidence interval of 390 to 440 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and ithe study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES___________________________________ _________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 135 8-2,1989. O T H E R ___________________________________________________________ Last changed: 6/26/00. 001750 BEST COPY AVAILABLE ENVIR0NMENTAL LAB0RAT0RV MICROBICS MICROTOX SYSTEM RAM DATA LR & 3 S & - 2 ? ^ i> H TEST snSTinrg F C ~ &Olo<L- TEST DATE: ANALYST: A jJScW v <3 u X rjL L rt ,5~OiL SAMPLE CUVETTE ... - - r - " TRITO" ------ FTRAmEADINGS FINAL ASSmA<YaCON1CLE,NTRATION READING 1(0) 5-MIN. 1(5) 15-MIN. 1(15) 30-MIN. 1(30} O- B1 BLANK CONTROL 77 7 ^ 6 $ Cl DUPLICATE R5" 77 7 ^. B2 IAS" L>3 5 3 rf C2 tXjplwCa^e l 1 bO 7 B3 5 -0 /oo HB C3 piic a i e TP s<\ V e) H (0 B4 C4 S~oo ^upiiVd.'fe 97 ZO\ vv 3V H7 37 31. 3 B5 10 0 0 9(0 31 * C5 \>>p\CCdA <7Z A<\ * 1 i /ellcM J t v f f lc t s * . . u .. t^ ilu e v tf: I . O r t & ^ 2 . ovy\4 _SA+pocxAtrctMSiyxp\vvH\-tscm. *w6f>rf r ^ ecl 0>^ ^ ^ ^ v>o coUr -ppArcmI- ie ^ c j ^ p ( M A ra b ic s A/jl< 5 '(u+ o k v Tnt+Cal *D i\oiiovi^ : X V + ta i e<>r\ceYvfr<L4iows Caere. d io o b te . + k e . F i n a l A sterf C&tycz.vW-rw,4 iorts ^ tn \\o v \e J i Abc*/?. tv.<- mi'-viA-C dLi'liHion^ X^'HbcftoeJ. Jk4cp 4W . ~F>p rov t o -u A \ AHclu>fe.`f-Vin ' fxi-^Swrca. (,5 0 /sro^ in + o + H c ld-uer-rotos &*i O O It o I LR G 1358-2 FC-206CE MICRQTQX'i r ) DATA SHEET 5-MIN EXPOSURE 6/16/89 SAB PAIR # 1 4--L7* Or 5 6 7. a CONC. r*zli- 125.000 125.000 250.000 250.000 500.000 500.000 /.1 0 0 0 . 0 0 0 7,1000.000 Io /It 90.0/ 63.0 9 6 .0 / 69.0 100.0/ 59.0 98.0/ 59.0 97.0/ 44.0 101.0/ 47.0 9 6 .0 / 31.0 .9 2 .0 / 29.0 G-OBS 0. 158 0 . 12.8 0.374 0.346 0.787 0.742 1.510 1.571 ' G-EST 0.151 0.151 0.333 0.333 0, 735 0.735 1.621 1.621 BLANK Bo/Bt- 95 ' / 77 . BLANK RATIO* 0.8105; : : " :' v 1 . ; -, 1 BEST COPY AVAILABLE p e r c e n t t&kfr- l ss" > | j i >>.Vff-'i-V-iv .` M XI Afe H 7 k -v- to k>\ . ........... . 1 ....... " " ' ' I " 1. EC 50 * 6 5 1 . 2 5 1 ( 593.503 TO 7 1 4 . 6 1 7 ) R 0 . 9 9 5 5 7 SLOPE 0 . 8 6 9 1 INTERCEPT = + 6 .4 7 8 9 -If . V. ; 1.; 1 .*. RES. 1 0.119 4!. 0 . 1 3 3 0. 076 4 0. 07S cv 0 . 0 8 2 0 . 080 7 0. 117 S 0.119 PROS. 8 100 33. 35 `V "v>TCOJ 36 77 : ai : 1-1- 11 1s > 1 1 l1 0> * i *1 i' . ; r i: -1 > 1 . =**'; ,77 '. V. ' i .. * ; , J 1> . 1 1 '1 -8 - i -- 7 1 L .. 10 a -- >mm` a 1,1 < . i( G --5 ----- ! 'G - ----- ;6- :i : 7 . r ( A a 1 n. , .< ^ ` .i/ - s < 1.4 ; ' :. .u.* ' /v , -- 4 --- ; :"J ;f ---------- - : 11 ;. ; , 1. ! : -V'i (;a >4. ' fi4 :v '-v< / ' :< v : i : " <. S 7Li a . > - y m v ,' /1 il a '.r / 7 : ``7 a I.-. a 11 - 2 + - ---------- :------------------- 45 L I S T 2RUN :3 L O A D Z O C T 6 , "L P FF K E Y E N, T1 LOG CONC. .............. 6 4 SAVE 7 T R0 N 0SC a II 8T R- ch*> 7 ;> '7.-' ` 7 . 5 7 R0 e b, f::- 001752 LR Gl358-2 FC-206CE MICRQTQX(r> DATA SHEET 15-MIN EXPOSURE 6/16/89 SAB VT i.' PAIR # 1 **. y, 4 5 6 7 8. CONC. tn ^ lL 125.000 125.000 250.000 250.000 . 500.000 500.000 7.1000.000 7.1000.000 Io /It 90.0/ 53.0 96.0/ 60.0 100.0/ 48.0 98.0/ 49.0 97.0/ 34.0 101.0/ 37.0 96 .0 / 22.0 92 .0 / 21.0 G-OBS 0.287 0. 213 0.579 0.516 1.162 1.069 2.307 2.320 G-EST 0.252 0.252 0.530 0.530 1.113 1.113 2.339 2. 339 PafCfcrH- 10 37 t 70 70 BLANK B o / B t = 9 5 / 7 2 BL ANK1RATIO 0,7579 DUCT nr y 1 ^ iM ' DLE (C g -o q s 0 *fr K V ;? 'r : v- ... - : ' , EC 50 = 451.134 < 415.191 TO 490.18? ) i\S Q t H A O ~ q < Q ) yr\g~ j , 7. ' i'.HTt\w&^: R * 0 . 9947 6 ' SLOPE - 0 .9 2 3 9 INTERCEPT * + 6 . 1 1 1 8 O'-... 1 ----- # * RES. PROS. ! 1 0 . 122 7i : 0 .1 4 5 -y 0 . 0 8 4 100 _:*o .4 0 . 0 8 8 36 X 5 0. 088 37 : 0. 085 7 0. 13l 3 0.131 34 0 > 8i 82 - : - l :> It.IST 2RUN v L O A D " C 0 N T 6 , *' L P T FFK E EN : 1 ' ' yt.' **r-- . i .1 I -- .6- 9 -- B-i *"*"* , 1 ; _1 * i .< 11 :i L Q G G A h , MA i-;v, F `r .* 5 LOG CONC. ,,A. 6 - hzwvm f!r'l*!?i-j I- j;i ' 4 S A VE '5 C 1 7 T R 0 N 8 T R ' 0 F-.. 0 SCR "W M v :l I'/:* v o lta i* LR S 1 3 5 8 - 2 FC -206C E MICROTOX S f d l ^ V A I L A B L E 3 0 - MIN EXPQSURE 6 / 1 6 / 8 9 SAB ^l-C'rV3;; .V.:l: /; ", PAIR # . CONC. Io /It G-OBS G-EST Perterrt- 1 . 125.000 9 0 .0 / 51.0 44 1 2 5 . 0 0 0 T 250.000 9 6 .0 / 57.0 1U. 0 / 4 5 . 0 4 250.000 9 8 .0 / 46.0 5 500.000 9 7 .0 / 31.0 6 500.000 101.0/ 34.0 7 X I0 0 0 .0 0 0 9 6 .0 / 19.0 8 %1 0 0 0 . 0 0 0 / . 9 2 .0 / 18.0 0 . 2 B2 0 .2 2 3 0 .6 1 4 0 .5 4 7 1.273 1.158 2 .6 7 0 2 .7 1 2 0.255 0 .2 5 5 0 .5 6 0 0 .5 6 0 1.230 . 1.230 2 ,6 9 8 2 .6 9 8 19 39 S& (? .7 /,^ 7 3 ^:/' 73 BLANK B o / B t = 9 5 / 69 '. : BLANK RATIG= 0 . 7 2 6 3 EC 50 416. 19J2: ' i,:< 390.084 TO 444.047 ) R 0 . 99664. SLOi^E'*8 0 .8 7 6 2 IIMTERCEPT fi -o e s ' ^ ; "A*i: t :/ P 3<J Q -- %*((>) tY\ M.--jK-^ 3~iF;j''j: !i "iit + 6 .0 3 1 1 ,,* . ' . ' :- *l\ '..r -i i *!' . s o*-t o RES. 1 0. 100 2 0. 114 3 0.0<b7 4' 0 . 070 5 0.070 0. 068 7 0. 105 . .1 PROEL 78 100' 35 38 38 35 86 87. ... i ^ : : ; : 0 : 1 . \ lo .1 1 1 > whwwQm j *---- ---- l 1i ; ---- .. , .-- r~-- 6--_ : :-v' \.7- L -1 4. 0 . ' ' ' < .1 i` . . I> ,, 1*. G e A --3 ----- /., i : . : ' :S r t : : / j.H v :-- '"4~ ; ' :, M ..'!v ^A . -;i- i-- 11, .IS T 2RUN CO N FK EN 3 L OA D T 6 , *' L P EY LOB CQNC. 4SAVE " 7 T R ;O N 8 T OS CR +.. u-7.; r!7. I l. ,. ! 5 ' ( R :o. E ; ' O i? E