Document 9JMQBeBkeeD7eLLaJ3Z4YkGBL

MICROBICS MICROTOX@ TOXICITY TEST TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acemtaytea;lso be referredtoas PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, or FC-109-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,sium salt,CAS # 2991 51-7) Remarks: The testsample isFC-109-X. Currentinformationindicates itisa mixtureof 19-23% testsubstance,61% water,12% 2methoxyn-eth@lethoxypropanol2,% ethanol,and 2-6% C4-c7 fluoroalkyl .carboxylatceompounds. Test sample was from 3M productionlot2. The followingsummary is abbreviated because the testsample isa mixture of the testsubstance in a waterlorgank solventsolution. Data may not accuratelyrelatetoxicityof the testsample with thatof the testsubstance. METHOD: Method: MicrobicsMicrotox@"BASIC" Procedure Test type:Staticacute GLP: No Year Completed: 1987 Species: Photobacteriumphosphoreum Analyticalmonitoring:None Statisticamlethods: EC2D, EC5o, and ECao values calculatedby statistical linearregressionp@ogram providedforMicrobicsMicrotox@ system. Test organism source: MicrobicsMicrotoxIDreagent Test conditions:As per MicrobicsMicrotoxmethod Replicates:Two RESULTS Nominal concentrations:Blank control1,25,250, 500, 1000 mg/L Element value: 5-minute EC2o = 170 mg/L 5-minuteEC5o = 650 (560-750)mg/L 5-minute ECoo = >1000 mg/L 15-minute EC2D = 140 nig/L 15-minuteECw = 410 (320-530)mg/L 15-minute ECao = >1000 mg/L Allelement values based on nominal concentrations. Remarks:Testinwgas conducteodn themixturoefthetesstubstancaes describedinthetestsubstance remarksfield.The valuereportedapplies tothatmixtureand notthetestsubstance. No attemptwas made to determinetheimpactofthepresence oftheorganicsolventsorwhat portionofthetoxicitcyan be contributetdothe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-109-X 15-minuteEC6o forPhotobacteriumphosphoreum was determinedtobe 410 mg/L Mth a 95% Confidence Intervaolf 320-530 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,utlacksanalyticaclonfirmatioonftestsubstanceconcentrations. There isa lackofcharacterizatioofnthetestsample. REFERENCES This studywas conducted by the3M Company EnvironmentalLaboratory, St Paul,MN, Lab Request number E2113. OTHER Last changed: 5/18/00 (41-;kLrl-o4l4-0 Lot;L) To AAtcro6tcxl S %-ie Tc!bt(2onc!. Pe ece t 19 L IL)@%I- 15 1A; f-,, 50 5C) 0 3 7 Ci 170 1 (q5 cr) _5 0 - 1.50) Lf o EC- @loco > 14DC0 Acf, rkiectSi,,ckckRpescirt%4,,@n Tt!stCone. P#.re-eyti 37 riIn'bti-tto r-, 3o-tAkA# n '5. 9 too Igo 3AO /00 E@ 5c> 000 cl) > loco (#-@zoq) ENVIRONMENTAL LABORATORY MICROBICS MICR'OTOX R SYSTEM RAW DATA LR P3 (Db-1, TEST SUBSTANCE: FC Ir'q- I TEST DATE It) - 1-5 7 ANALYST: SAMPLE CUVETTE FINAL ASSAY CONCENTRATION IL --MTIAL READING 1(0) FINAL HI@AUING5 5-MIN. 15-MI!I. 30-Mltl 1(5) i(lb) fl@30) Bl BLANK CONTROL 7(- 82 C2 Icate 83 C3 B4. 50 C;, C4 .BS. ICCC> CS Ct /oo 97 S7 q6 qq q3 q Lt 31 /oo .27 111 500 m,@ &ct, st 4.7 w,*i.@ ;i@ ro%aA,,L@tJ.C, ;Lq p 0.7 AJ4. cc iicpi ba: water IQ&- A) ani wtire Aer&ktr$Jvc%Lr4tj tke- rh -U1.2a- n d @TICWBICS IIIMMXR SYSTIM BLANK ILATIOS* Irinal Initial HICROTOX R DATA.RzwcrioN FC -109-)( F-r-Surqdc+dh# BLANK CUVErrE 5-MIN. HE BAIIQ IQIFFERFHR-E ETANit 15-MIN. R&TTN InT -Bi cl- 7Li? 0. cc T (o *The blank ratio# for 81 and Cl should agree %tit@if0i.02. LR E-.211 I)ateJO -15-.QZ Analyst pt.;Le) ;L See pti -Carsfcm 5owticel prep. ATA%IK RATTN MEAN BWK ILATIOS BI BK RATIO + Cl BK RATIO 2 5-Mim 7 MORMLIZED LIGHT LOSS (PEFFECR) Mean Blank Ratio x Initial Sample Reading Final Sample Reading PLE CUVETTE st Concentration 2 ;t5- mA)L goo loi>o 5-MIN. 0.;kl7 a9 o ;t9 7 o 355- o blob 0 7-'32, I. 1053 I- &,gLi 15-MIN. rfo. 3al .0-35 0.9si .3-810 3. C-71, 30:WI-N.-Ir Z LIGHT LOSS (%A) LE CUVMTE est Concentration 2' X 100 15-MIN. 2$1 30-MIN. Z a q0 ly ECRIVE CDNCEWMTICNNS (ECs) mr4 Ec's 5-MIN. EC 20@ 50 (95% ConTir..Lisits) so > loco 7q @Calculated usifig'*P.C*data pairs.) 15-MIN. 30-MIN.