Document 9JJp46ZMQy1Oo9RqdxXLr8JxL

I*m H askell Laboratori* of I n d u s tr ia i Toxicology Y lla ln c to a , Delaware* V*w tliS cori. 773789 ENP 010176 I ri. T s x l e l t r ad ta r * r s o f Ia rt* j ad IU ltd S ubataaa, la c la-d lBaydPmocMarabtbooada *l af iAa iar.l j i l a top S a ls |ta a t d I 7 too O i u ia iK , k9 . | 7b*S. * The to x ie o lo g le a l txp trin c a ta oa th ia probit otro c o rri o u t by D r. J* B* Toulgar ad Dr. A* J T le a ia g o tha D eporta o T d x ico lo g y and th a p a t o l o g i c a ! s t u d i t i e t r a mudo by D r. H. Head, J r* o th a B ap srtsaat o Pathology o tha B astali Vaborr The a t t i r i a l vus i n r i s tifa , t od w itb o r a i s d a ia is tr o tle a t; r -ti, a p p lic a tio n to th s tia o r a ta , and ia in h alatlo a tap i a c a ti* The l o t t a r o t r i p la n a ti l a auch a vay ss to approach c lo se ly s i po ssib le th actu al co ad itio as oaeouattrtd ia th o f th is m a te ria l ia transform ara* in o rd tr to tra in a ta th ss 9 haxard hieh aay b t tncountartd in i t i uae io r th is purpost coaparisoa* a d d itio a u l t e s t i ere sane rtgtrx. th to x ic e f i ir a in h Ic tic a o Tapora fro a Pyranoi and Iran afo raer O li ( inghouse)* Za td d itio a , s method v a i eorked out io r th d et m ination o Z aerteea ia air* t T T fie t o f Ora? srtiiTii i t r u t l o n Za o rd ir to determ ine th m iniasi fi-ta l conoentratloa Z n erteen f o r r a t i , 100 a o io a li te r g ire n by stoa&ch tube s d o s e l o f Znerteen. n i indicuttd in th tuble* 773790 Dose /kgs* G E N P 010177 .Ho. O a n i nuli treuted Ho. o dead Time of d^euth Per c er n o ria l- 1*0-1.49 1*5-1*99 t*0-t*49 .1*5-2*99 n k6 24 1U0Q 20 59 4 6 4f * vithin 5 2 days 1 5 24 2 1 10 9 4 1 2 15 * . 2 21 1097..: 20. 50. 75. ft ' '* 773791 ' In th is , u t la sub sequent experim ents, a l l rut shoved signs or discomfort lnaedlatsly follow ing the adm inistration, l a t e r they bectne depressed end re fu te d , fre q u e n tly , r a te r and food} n t h in * fee hours they beeto e week end stupe ro u t, . a th a t they were f in a lly unable to r ite * then th is stag e ** reached they in v a ria b ly died One 01* th e s e r a t s was found to s u ffo r from albu&i&nrlm, one devtloped tr e a o r s of the head -nd fo re-lim b s, and one shoved sever* hemorrhages fro a the i n t t i t i n a l tr a c t The ta b le In d ic a te s t h a t w ith o r a l a d m in is tra tio n th e minimal f a t a l dose o f Z&erteen f o r r a t s , which k i l l s 71 p - * s." # cent o f th e anim als v ith ln o days, la 50 to 49 cc. per k ilc gran body eight* . . --~ Upon p a th o lo g ic a l exam ination o f 0 o f th e s e a u l a - l s , th< t h a t hod d i e ! sp o n tan eo u sly shored c o n g e s tio n of th e luaKS_ * t a s combined v ith edeaa una heaorrhu*e In se v e ra l in stan ces o f the anim als had n o ttle d g r7 or yellow liv e r* und i n th re e Instances there vas a d iffu se p a llo r v lth d e r a te yellowness In 5 o ut o f 6 an im als s tu d ie d m ic r o s c o p ic a lly th e l i v e r shore narked and ra th e r ex ten siv e h y alin e n e c ro s is in the c e n tr a l ; of the lobules; the liv e r c e lls In th is area revealed fu tty < g e n e ra tio n o f th e cy to p lasm ; and i n 1 a n im a ls t h a t d ie d 5 i &: 54 hours a f t e r th e tre a tm e n t th e s e c e l l s c o n ta in e d in c re a s e d , numbers o f a i t o t i c fig u re s In th e m a jo rity o f the anim als 1 mucosa o f th e i n t e s t i n e mas c o n g e ste d one: th e f e c a l c - t e r i a l the ln te stise e contained' old blood; and in one instance ire s blood as found in the low er th ir d o f th e ile u n , lover culoe 1010 d H 3 0  and alcool* t i ero copi Daily th te u e h of ti a a ln a ls i p re se n tad ty p ie a l fcemorrhfic erosione of th Bicoaa; maothe? shoed heoarrfaasic a ra ta in th s ic osa; and in th re sa in in s anim ala p o a ta o r ts a mchancas id mot a llo an exaet l n T s t lx * t i a In o rd tr to fo llo up th ten ie e f fs e t of Inartaan on th liv er," a 20 t i t a ra ta v is tr e ite d in th sis e vty i s in g le o ra i doses o f 115 to 120 ce* per fcilotram body Teigfct S iz o f th e s e e re fc ille d 5 , 8 , and 11* day a , r e s p e c tlv e ly , a fte th adnlnletr& tien* Upoa p - th o lo g ic a l e z a sin a tio n i t aa found th u t ta o of t 6 a n s a l a fc ille d a f t e r Z days )wd blo o d y m a te ria l* i n t h e i r s to n a c h i tn d i n 50 p e r c e n t o f th e a e anim ala th l i v e r v:aa a lic h tly yellovr in co lo r anc the liv e r c e lla ore la rc e and < tain ed 'n u aero u a n ito a e s and o c c a s io n a li/ th e re va n e c ro a ia c e lla im aediatcy ato u t th cen tral v ain a. Anisis k ille d a 8 days p .reaented a s i i t h t e n l i r c w e n t o f th l i v e r t i t h a qv tio n a b le y e llo c is h c o lo r, th live*/ c e lla cere la rc e , those th c e n t r a i T ein a c e r e darle, and o c c a a ie n n lly th e r e a s sos*, f s t t y d e g e n e ro tlo n o f these c e lla F.ats I d l l e d a f t e r 11 d i shoced no im portano cro ss pitholocy L iicro ac o n ic tlly , a l l tio n s shoved u s lic k t s re llin g of th liv e r c e lla r i t h occt acu o lix atio n o: th e c y to p lis n Ko o t h e r organa sh o re d pat changea re fe r a t i e to th ex.:oaure # In o rcer to study tho e ffe c t of rc-igLtsd o rs i td n in ls: of In e rte e n , Z tro u p e o f 10 ra te e tc h a c re tre a te e in th e i a t wayi 773792  773793 Zb* f i r s t (re a p n e i l T i d f r e a 0.S4. to 0M c c . pr n-ti* 6fo r eoasoeutlT* dors, la s* of tb*o di*d oad or* k ille d a f te r tilt 3rd treatam at fo r pathological examination. secood roup m s fo d dost from 0*63 to 0*83 c e . par k ilo * * Tva d i t d fo llo w in g th e te d d o st and ft v e re k i l l e d f o r autopsy a f te r tilt 3rd adm inistration* In the th ird roup tbs dosts ranged i r o n 1*C3 to 1*78 cc* per k ilo * Oat r u t d i t d a f t t r tba l i t , tb r te a f t t r tb a a d , and fo u r a f te r th e 3rd trea tm en t, ar the rem ainia3 anim als ta ro k illtd four days a fte r the 3rd trs aeat* Upon p a th o lo g ic a l* e x * a ln ;.tio n th e l i T e r i o f th e r a t s o f f i r s t roup appeared somewhat lig h te r than u su a l and one sho* la r g e y e l l o t a r e a s ; th e r e was s d if f u s e m ild s p e llin g o f th e liY cr c e lls r.ith tone polyehrocasia; occasionally tic. c e lls : soac v an u o ltr ch-Jiges oi th e cy to p lasa, but in general th e se *c h sc ^ rs were s o t v e ry sev ere* In 4 r i t s of th e t h i r d roup * died a f te r 3 treatm ents the stoaaebs vere d ila te d w ith food i n t r a o f th e s e t h e r e t a s a ls o some o ld b lo o d p r e s e n t . U ie r sc a p ic a lly , one r a t has s n a il inflammatory u lc e rs in the fo r stomach and an o th er shored hem orrhagic ero sio n s o f th e pylo: / i l l sh o red soac h y p erem ia o:* th e l i v e r and ii> one r a t t h i s t o f n o t t l e d a p p e a ra n c e . M ic ro s c o p ic a lly t*. 0 r u ta shoved sweJ o f th e l i v e r c o l l s v i t h f a t t y d e g e n e ra tio n #o f ta o s c i n th e p a rts of the liv e r lo b u les* Tto o th e rs presented c e n tra l n o f th e l i v e r c e l l s a l t h h y p e r p la s ia c;* th a e n c o th e li a l c e l l th ese a re a s , one shoving in a d d itio n , numerous m ito tic f lg t GENP 010180 773794 th e l l r e r c e lia * Of ru ta th l e h a e re U l l e d < daya f o llo T ia ; th e Srd n l s t n i a t r e t l o n , x i h&d a w . 11 aoorunt of b lo o d l a thv e s t r e co aten t* *Xht l l r c r a o r b a th a r l a s l s *p:ered a d e r a t n la rje d and a le ro e c a p ie a lly th e re va* a ( i n i r k U t K e v e llln s or tb*> l l r e r c e l i a e i t h s o te polychro& aslt Ono r - l a u f r e r e d froo fa irly en trel recuolr degeneratlon of the lie e r c e ll and eaother proaeated Tacuoler c h u c e a ln the c e n tra l p t r t o: the lobulea n o rd er to coapare the to x ic e f fe c ta o f Z& erteea v i t h trlchlorobensene and tth y ltetra-penta-cblorobensene, a rou of- 10 r a ta wls c l T*Q d a ily do cta o f t r l eh lo ro b entene ra n g in g frosi 0 S6 to 0 SS ce* p e r h i l o body relght* One d ie d n f t e r * ** * I s t , fon? n f t e r th e la d lxi T oar e f t e r th e Srd a f c l n l a t r a t l c th t rtk ln ln s rat tas h llled 4 days f^ilo -in : the Crd md 1; tres-toent* I t ap p tars, th e re fo re , thi*t dn ily doscj of 0*36 to 0 .5 p er h i l o o f trlc h lo ro b e n s e n e h l l l e d SC p e r c e n t o t h t i-nln c ith 2 coses, eherc&s i t r a a o u n i th.t clases o f 0*61 ta 1 per hilo of ethyl-tetru-rscatt-cy.lorobergene. tlT cn ln the : ouy, h llle d only 12 p e r c e n t, t a l Xnerteer. ln o sc3 of l . h l 1 . 7C c c . per h i l o , tveu ln th e sene * cy , c i l l c d 0 p e r cor tfcn ra ts * Upon p c .th o lo g le a l e x a n la n tl n , c n i s a l s r.l.ich v e r o t r e r l t h t r lc h lo r o te n s e n & t:iu v h ic h d i c -1 a -ontciicously sho^-ed pnsslTc congestin o f the lunes and th e llT e r; th s stonuch i M **t*!t.ended v l t h fo o d ; l n f o u r s o a e b lo o d t a s p r s e n t 1 e 773795 ( a s t r i e c o n t n t s and la, one i n s u a c i in e r t va* 4 haaarrhagic r a tio n o r th e mieosa* Za tr o r a ta m e l i v e r shoved Irre g u la r n o ttlln d * L iver sectio n s of a l l ualnals shored necrosis of th e liv e r c e ll* around the c e n tra l veins and those in the out; p a rts shoved vacuolar degeneration In ra ts vhleh died S to 4 days a f te r the f i r s t tr e .tn e a t th ere as soae en d o th elial h y p e rp la s ia I n th e a re a s o f n e c r o tic l i v e r c e lls * One presented an lnereaso In the nuaber of a lto tlc figures In the liv e r cells* Of th e 7 r a t s tr e a te d v l t h e th y l- tq tr t- o e n tL eh lo ro b eset th e one v h le h d ie d sp o n tan eo u sly a f t e r tho Zrd dose showod cc c a tio n and h sao rrh ag es o f th e lu n g s, g en eralised p assiv e cc: geatlon of the sp leen , bone a a rro r ana other v iscera, cisten- stonsch, and aoderate yellow a o ttlia of the liv e r, the liv e c e ils being n lld ly sv o llen and shoving fin e vacuolar decener The a n ls a ls w hich were k i l l e d s h o ts ! only a v ie s tic a a b le e e l s e n t o f the- l i v e r and kid n ey anu u s l i g h t sw e llin g o f th e 11 c e lls T.lth o ccasio n al vacuolar d eg en eratio n , Lich changes eztrenely slid * I t appears* th e r r f a r c . ti.a t In ertee;i lz eonr'lccrablv 1 t j r l c than trlchlorobt?neenc. t he ' Tut s i dose '.1 t h o r hd.sialstv>:tloR > k l l l i n r 73 p e r c e n t o r th e a r .lc a ls . b r in e 1: S0 cc i a r k i l o T i-t:' T heresa e th Y l-ty tra - c e n ta - c h lo ro h c n s e does not i.jiic. r to be ne-rly es to x ic r i t h o rn l trisin l ir tr it The n sth o lo * -lc:.l chan re a observed a f t e r o r a l L d ^ -ln is tra tlo r I n e r te c a aoc*e` r to he f a i r l y c o n s i s t e n t and obvious.* L a r r t GENP010J82 7 * i* 773796 i Me* im gaB.f 9ti" la or a n , cam r - |'n Tl th Tlieiw. ! i w w t K t t i n af t w U t p It* nepoia fcrwad th> rtla. a M t a U y . B l e w ' af tt m t r t e uoo. rebithii das caua ltM * ` *1^. < tatrlc Irritation end feooitle flgteaor^tloa Tltfa of rceenoratlan. th lattar It flocldedly arc utrjeod f t r tabt-dara. tni eftcr loro dart no doflnltQ llror ^*t*alag-r cr b* srosent. It ihrald alio be tainted out 8b<b h lrte doact atj .r to bp aare lnHirlaua' timn neb doiei In fraction. p r fe e t of J t l l c a t l n n ta tb e Bteta. lea abite* rets **r* treated dally with an explication oi 0*50 about ee. or Inerteeu to the shored skin of the back, P - * cautions being taken to pri-Tent e b so r/tio n through the g a stro i n t e s t i n a l t r a c t by li c k i n g u id by in h a la tio n * A ll r a t a l o s t r s ig h t during t h r experim ent b u t they shoved no to x ic si<pis tsid e fro a a s lig h t re stle ssn e ss e t the beginning of the ex perim ent. At th e s i t e o f the a c n in is tra tio n th e sk in became d ry , thickened and sca le d t i t h o u t tn y f is s u r e s b ein g formed* S im ilar re a c tio n s itere observed on the huntn sk in e sp e c ia lly on th e h an d s, ch aek s and fo re h ead * OnG u; t h e r a t s c lo u a f t 11, and th re e a f t e r 15 tr e a tm e n ts , tle r e u tin in g b ein g k i l l c la hours a fte r the lfcth a p p lic a tio n . Upon p a th o lo g ic a l e x a m in a tio n th e l i v e r o f a l l a a l a a l t vas found to be d ark gray b ra ts i i n c o lo r tr ith n o t i li n g on tl GENP 010183 urfees* In those rats *hieh died epoatsaeously there vue fotty deeeserstiOB or the liver cells *lth necrosis or those round the eentrsl veins and in iminuls killed tt the end oi the oxporlacnt there ves c sild svelllac of the liver cells with occasional vacuolar decener^ti-n is aoee. Kieroscopicslli 4the skin r.t the site of applicetioa of rets shooed hyperker' tlaisetioa eith oeeasiocuj. blister foraation, one shove perifolliculitis, one ^rperplesie v* the eoitheliua rsxher tha 2hyperkerstiniutios, and in rets there- was e sild fibrobler- proliferatlon vith soae round ceil infiltration in the uadsrl: connective tissue* m I t assf& ra, therefore th s t the contact of Inerteen l t ` the kin doe5_n3t only errase locr.l re a c tio n s such ns drv n ess. thlcbeninsL uvi p e slln g o f th e e k la . b u t a ls o g y s tc a le e f f e c t as In d lc fita d b r _ t h r I n ju r io u s e f f e c t on th e l l T e r * hfft-ct of Inhf.latlan of Tcoors la eru lu k tin c the to x ic e ffe c to of in h a la tio n o f Tk?o: l i b e r a t e d f r o s I n e r t -ea u t d e r a t e d te n p e r n tu rc s u t t u e p t s rerc antic to reproduce c o n d4 itio n s &oru sererc th tn vould be s e t in the o rerh ck tin j of a tru n sf orner# la these entrine rk ts isere exposed in u U n ite d pace o f .bout I cu b ic fo o t ca p acity to the Ti.poro o f th e b o ilin g m a te ria l tfhich enturr d ire c tly in to the chuober through n h o le in the bottom . I: each experim ent h r u t s * c r* exposed on ho to G6 o c c a s io n s a0 sa n u te s , t ic c ch*lly# to v ap o rs f r o s X n erteea, P y ran o l, GENP 010184 773797  T ransform er O il (Teetiagfaouse)* D arlas t h i s H ju t^gy glowed i r r i t a t i o n o f t h t s h in , which appeared to b# most marfced on th o se p a rts o f tb s body n o t p ro tected by b i i r , sau which per s i s t e d f o r some tin a f t e r th e exposure* P yranol appeared to product tb s a o s t o f f s e t i n th is re sp e c t, followed by X aerteen aad T ransform er O i l , i n th s o rd e r given* Xu o p p o sitio n to t h t k r a ts sxpossd to Pyranol and Transformer O il, 3 out of 6 re ts exposed to X aerteen l o s t w eight, b u t no o th o r toxic slg n i sere observed* I t i s q u ite p o s s ib le t h a t r s t s exposed in t h i s wa7 absorbed son* to x ic m a te ria l through the g a s tro -in te s tin a l t r by lic h in g th e f u r d u rin g o r a f te r th e exposure since the s a t r i a l had a tendency to condense on th e w alls o f the chamber a on th e s u r f a c e o f tht* a a la a ls * The n a x isu a c o n c e n tra tio n o f I n e r tte n to -h ic h th e se r a t s to r e exposed was det*:r-incu as ; 0,5 b j* per l i t e r of a ir# Upon p a th o lo g ic a l e x a n in a tio n o f r u t s exposed i n t h i s on 1 4 , 25 and CG o c c a s io n s to I n e r t e e n , no a e f l n i t e p a th o lo t- signs could ho observed in th e l i v e r o r o th e r org-ns which c be re fe rre d to thu exposure* Two r a t s t r e a t e d w ith 41 exposure;. to th e vapors o f ? shoved no d e f in ite c e llu la r changes in th e liv er* wlthough 1 a n ia a l th e re was an in c re a s e d number oi m ito tic fig u re s in liv e r cells* * Two r o t s ex p o se s ou +4 w acasiu n s tu th e vapors o f Tri f o r o tr O il (tc stln g h o u se ) showed uo d e f in ite p a th o lo g ic a l changes of the in te rn a l organs* 773798 10 Za o r ttr to tujr t&t i f f i e t i o f jSB SlB m jU SSG U Sj 10 rota v*rt e hour* d illy oe b Oaf a a a fo r 80 'Oafa to eoaeoB tratloaa o f 0*08 i ( . to 0.0 8 me. o f L a ir t per l i t e r of *lr* they ehored no to zle ao r pathologies! signs r e f e r a b le to th e exposure; th e r e s e r a bo d e f i n i t e changes o r th e rod blood corpuscles o r th e b u j( la b ia She animal* a e re h il le d S days a f t e r 0 exposures* ** Tt appear. .therefore* th at n eith er frequently rqptated s h o rt exposures .fo r fcO g ln u te s each to c o n c e n tra tio n s o f 0 ^ u: of Tnerteen per l i t e r a i r , nor continued exposure to concentra ti e n s o f OoQS to _Qi,09 sr p g r l i t e r a i r ccuse a d efin ite* to x ic effect.o n the llT tr * O e te rs in a tlo n o f C oncentration o f In e rtc e n in th e i=.lr* In order to d etercin e the co n cen tratio n of Xnerteen in the s i r , the follow ing B tphtlo& etrlc sothod \a s .sorlcea out a fte r seTerol other procedures had been considered* A d e f i n i t e r o lu s e o f a i r to b e t e s t e d i s d ra m a t a ra* of about 1 l i t e r erery 4 ninutec through a te s t tube co n tain cc* o f eth y l a lc o h o l, in n ersed In d r / ic e -a c e to n e f ix tu r e o r c e r t o p r e r e a t th e e v a p o ra tio n o f th e a lc o h o l. At th e enc th e s n s p lln g 1 c c . o f th e a lc o h o l v.aa n ix e d >i t h 10 c c . o f < t i l l e d r a t e r and th e r e s u ltin g t u r b i d i t y was n ea su red t i t h photo e l e c t r i c c e l l , usin^. a s o d iu a T;*.por 2,aa? ** th e coure lig h t* * The tu r b id ity o f unicnoun sam ples was conpared to th e 773799 GENP 010186 u M* o j t r t M t hs t b i t t h i e a u n t n t t g u o f laertM C. la ir crNai pitty t t the ttiUsci ftsa the sour*. ef their production. Za order ta stady Turiatia&a of the eoneaetratlou ovar a parlad of tli. ad aith i i i m t t u p i n t u n i , curroat of Z lltcr par minute of air vas pasead orar tba surface of laar- 10eaa of bout uara lachas, eosUlaad la a flask aad tbaaee lato a bail Jar. Suplas vare takaa fraa tba Jar at Tarions interrala aftar tba flov vas startad aad *itb Tarious te a p e n tu Zba follovlax eeaeaatratleas vara dataralaadx Tint of aapllXLf la hours Concentration of t r l ch i orob enzane l a *4 per l i t e r Tape ra tu re of t i r n i f'clhalsohro b onCz e. ne Trlchlorobenren e * . 6eyV 1 4 1 00..2691 0.60 1.68 11..8700 e23s 60 6900 X nerteen 4 0.58 6 This ta b u la tio n shoes th a t th e d eterm in atio n s a&ie a t &3C. and K ith th e 6 h o u r Simple v e re a l a o s t th e ame f o r trlchlorobenzene (0*60 ag . per l i t e r ) end In e rteen (0.58 eg. per lite r ) T his s u r e s t * the p o s s ib ility th a t the bulls of th e T apors liV u r o te d f r o a I n e r te e n &t t h i s .te m p e ra tu re nay be trich lo ro b eazeaej so th a t th e hazards fro a in h a la tio n o f Ine: te e n Taoors t r e I d e n ti c a l r.lth th o se fr o a th e In h a la tio n o f 773800 GENP 010187 ta r b id lty preduced by teems iu s d f d i mads sp o f tr ie h la r e b a n s # and tb y l A lcohol, Ib is method gave M tis fie to ry r o a d i e s o t i r a r u i ^ i from 0.80 to 8 ,0 mg, p e r U t i r o r a i r and wham to o o r i g i n a l d ilu tio n s v e r t ta k e n l a t o acco u n t, i t * sac e f f i c i e n t l y s e n s itiv e to d e te c t tuan t i t l e s ae low as 0,08 mg. o f la e r te e a 'p o r l i t e r o f U r . th e method I s n o t p ee lflc fo r the various seahers of the halogcaated hydro, carbons but sin ce the vapors lib e ra te d fro a Znerteen c o n s is t m ainly o f trlc h lo ro b e n x e n s , as w ill be shown below, i t L y b e used fo r the d etcrain atio n of Inerteen vapors in a ir. Zn o rd e r t o o b ta in in f o r a a tio n re g a rd s changes o f th e concentration of vapors in a ir with the distance fro a th e source of p ro d u ctio n , samples of U r were taken in a c lo se d space a t varying distance* iron b o ilin g Inerteen m d the follow ing co n cen tratio n s were determ ined: D istance (Inches) C oncentration . tg , per lite r 14 4*50 20 1,86 9 1*14 86 1.14 .hen sam ples were ta k e n a t v a r io u s d i s t a n c e s 0T@r an open beaker- o f b o ilin g In e rte e n th e r e s u l t s were t-s fo llo w s : D istance* . (inches) C oncentration mg, p e r l i t e r 5 0.56 10 0,48 15 0*24 0 0.&4 GENP 010188 773807 13 121trteiil#rob< U K ia Xa ordar to e o a firu te ll, ec . of Xaerteea v e re subm itted to f r a c tio n a l d i s t i l l a t i o n and the follow ing r e s u lts vere obtained F ra c tio n 1 8 4 Residue Temperature of d istillatio n 240 . 40-284 884--2B0 ESQ-300 above 300 Tolunt eolleetod cc*. S3 a 48 80 ISO OpraoTc lltfTl e B oiling > o in t* C * of fraction 1*488 1*478 1*483 1*S77 1.S9S SO&S-B21 40 69 Bluet th e ap e cifie g rav ity o r trieh lo ro b en se se i s 1*436 and the b o ilin g p o in t 0SC*-219*C*, i t i s e v i d n t t h a t up to 240C. th e v ap o rs c o n s is t e x c lu siv e ly o f t r i chi rob ea sene sad t h a t Tor t h i s re a s o n th e above t t a t e a e n t 1 j u s t i f i e d . n s s e lT t th a t fa r taoat conditions the h&sards fro a vcaore o f X aertecn Id e n tica l v lth those fron trlchlorobenzene* Besuae The ex p e rim e n ts d isc u sse d i n t h i s r e p o r t show t h a t w ith o ra l ad m in istratio n Znerteea i s considerably le s s to x ic than triehlorobenxeae, the f a ta l dose k illin g fro a 70 to 75 p er c e n t o f th e r e ts b ein g around 3*0 cc p e r k ilo g ra m bo freight Lad 1*4 to 1S pep kilogram body w eig h t, r e s p e c tiv e ly F a ta l doses t r i l l cause p a ssiv e c o n g e stio n o f a l l v i s c e r a , er ten siv e degeneration of the l i v e r , ad u lc e ra tio n o f th e ga: aucosa* TTith s u l l c t h a l doses th e s e e f f e c t s a r e l e a s se v e re th ere is evidence th a t they tr ill h e a l then no fu rth e r expos occurs GENP 010189 ^3a2 14 Continued ap p licatio n o r X nerttea to tb s t t t n of ra ts end bonans eaujos dryness of the sJdA, thickening and sca lin g ; and the observation* tad in -ra ta in d icate th a t s u ffic ie n t m Q uantities nay be absorbed through the skin to produce in ju r i ous e ffe c ts oh th e -liv e r Repeated in h a la tio n fo r ab o rt periods o f concentrations^ o f 0*5 at* o f Z nerteen 'v ap o rs per l i t e r of a i r , o r re p o s te d . in h a la tio n f o r 6 ho u rs o f c o n c e n tra tio n s o f 0Q5 to 009 as per l i t e r of a i r causes no d e fin ite in ju rio u s e ffe c ts in ra ts Vapors lib e ra te d fro a b o ilin g Pyranol, Xnerteen, and T rans- * f o r a e r O il (TTsstinghnuss) a re I r r i t a n t and th e i r r i t a n t a c tic decreases fro a Pyranol to Transformer O il (Y stlnghouse), Inerteen being interm ediate in th is respect Zt has been shorn th a t vapors lib e r a te s from Z nerteen under c o n d itio n s approaching th o se as n ig h t be* e n c o u n te red L transforners co n sist naialy of trlchlorobenzene a simple nethod fo r the determ ination of Z nerteen ( t r i chlorobenzene) vapors in a i r i s given I f 10 l i t e r s o f u i r are draws a t a ra te of about 1 l i t e r every m inutes through t e s t tu t .c o n ta in in g 1 cc of e th y l a lc o h o l, cooled In dry or in an ic e s a lt a ix tu re , and the alcohol i s then poured c f u lly on th e su rfa c e o f o fo e cu b ic c c n tia e te ra o f v a te r in a t e s t tub*, .a r i n g o f t u r b i d i t y i s produced i f th e concent t i o n of X nerteen ( tr ic h lo r o b e n z e n e ) i s o v er 0*03 iag. p e r 11 of air I f under these co n d itio n s a v k itt rin g is produrec GENP0i019u 773803 tha a ir t u t t d shsold ba r if ir d it t i t t a u x p ie i u d ba lap potad by s o r t t f iM t lT i T n t ila t t . 2t ** found thar lb eeacaatratlsa of Snarl (tri- 14eklsrobaoxaea) In ft cloud tpaea inch* aboTt m curfua : of boiling Inerlaca S i 4 .8 a g . par 11tar aad In an .open apaca 0.86fi Inch* abora the'aurfaea of bolllag x&artaaa lx xj. par ^ eri obi t-a tanparatura la a tracaforaar, landing In 0 1 .9a elosad rooa (rault), rlxaa to C. tea air nay contain : per l i te r of air* F ro a th e bsiI oaI experim ents^ r e p o r te d , i t appear a t h a t c o n c e n tra tio n s below ^0*05 ng* p e r l i t e r o f a i r w i l l cau se n o to x ic sig n s o r syup tons even t i t h continued exposure Regards O-^^^ntlYe n essu res. the follow ing nay be sugge s t appear:- to be o f pureiiount in ? o rt:n c e th a t th e contt: n a tio n o f th e a i r be reduced t s c o n c e n tra tio n s below* 00R eg* l i t e r o f s i r by adequate fo rced v e n tila tio n a t th e s i t e o f th production of such vapors* G re a te st p e rso n a l hygiene i j n o t le s s im portant* Ccnta. n atio n o f the skin should be avoided by se a rin g proper protec g a m e n ts such a s gxOYes, c a p s , one. c o v e r a lls * I n c a se o f tc : o r t i t h ex p o su re t o h ig h e r c o n c e n tr a tio n s o f u ic above 0S n. l i t e r , r e s p ir a to rs o r open a i r masks should be worn. She *k should be kept in x acu lu tcly clean and ointm ents such us lano o r Iq u a fo r should be a p p lie d to th e sk in a f t e r washing the b in vier; ci th e heputo to x ic a c tio n of th is conpound, l r v ld u als s u ffe rin g from in ju r ie s o f th e l i v e r , s y p h ilis , and GENP 010191 773804 d iseases should be excluded from op eratio n s in th leh L n srte m i s handled* . Yorxars handling Xnertaen or s lth frequent exposure to i t s vapors should have perio d ic exam inations, special a tte n tio n should he psid to th e ir n u tritio n a l co n d itio n , ana the e o a d itic o t the liv e r a s / be checked by a c te m ln ln w the ic te ric index in the blood, end the u ro b ilin end u ro b ilin o g en excretion in th e urine* Regards th e t r e c ta e n t o f to x ic e f f e c t s produced by expos* to In srte e n , i t appears advisable to in crease the re sista n c e c e th e l i v e r by carbohydrate d i e t ; a d m in is tra tio n of g lu co se end I n s u lin s&y be e d T ise b le , u n i duodenal la v a g e w ith u a g a e s iu a s u lf ite any be tried * Local i r r i t a t i o n o f the skin produced 1 In erteen should be tre a te d w ith bland ointm ents a f te r the ton: c a te r i a l h-s been re a c te d b; in te n s iv e 'w a s h in g and scru b b in g * In case In e rtu en has been taken by c o u th , th e to x ic n a te rls .1 s!:ould be rtx o v ei fro n th e stocach by e s e tlc s ouch as soap m o r cu stard t s t e r , and th is should be follow ed by g a s tric la v : with a suspension o f a c tiv ated charcoal in u a te r and sa lin e c a th a rtic s such ns Epson s a lts * w IS *33 4* G BTSP 0 10192 773805 THE TOZXCOLOGZ Of Z1X3TZSI AMD 8ZL1TXC SUB- STUiCZS XK1MXK A METHOD w7 JJUL1SIB |X)F KXIACTMUZD HTDHPCIE, t t i . Mm J* T l a i f i i j I . fi. The Toxioolocy o f Xnertaen v u i A T i i t i f i t i d by o r a l ad m in istra tio n , sk in ap p licatio n , and in h a la tio n experiments l a r a ts * Xa th e l a t t e r an a t t e a p t aa Bade to in v e s tlx a ts tha substance la a ay re la tiv e to I ts usa la tran sfo raers and to determ ine tna esTisua hazard th a t s ig h t a rlsa should ta a su b sta n c e ba us ad f o r t h a t purpose* Tha a ln la u a f a t a l dosa as d a ta ra la a d by o ra l adm iaia- t r a t l o a and th a a f f a c t o f ra p a a ta d o r a l d o sas as s tu d la d anc a compared w ith the a f f a e t of s i a i l a r dosas o f trlehlorobenxeni and e t h y l - t e fce p e n ta-ch lo ro b en ze n e. X aathod of a ir a a a ly s ls 'fo r the halo ctna tad hydrocart* *as d ev ised and a i r analyses era Bade to d e ta ra ln e the con* e a n tra tlo n s of Xnerteen In tha a ir n ear open and closed eout a rs whan th e su b sta n c e as h ea te d to v a r io u s te a p e ra tu re s an vuan sanples vara taken a t various d ista n c e s fron the source JL s ta te m e n t o f th e p ro b le m s .in c id e n t to th a to x ic o lo g y o f X n ertaen and th e answ ers to th e se p r o b la s s i l l be found i n co n c lu sio n s and sunnary a t tha and o f th a paper* Inerteen Oral A d a t n l s t r e t i o n : * 773806 GENP 010193 (1 ). Single Ooata. The su b sta n c e was given i n s i n g l e g ra d e ! d sse s to XAVBrtl rroinS of rats to- datinafna t>i* fatal dose. GENP 010194 JAlSkiLl 0 HIM1KUM PAUL DutiE OP 1NKHTEEM M ill B1MQLS UHAL APHIMSHUTIuM____ _ fc Doao /in 1 .0 0 - 1.>49 1 .9 0 - 1,.99 9 .0 0 - 9. .49 9 .9 0 . 9. 99 9 .0 0 - I . 49 I* , of Aalaola Iraatad M 94 10 90 90 Ho. of Paatho Tima o f Pootin & 4 died w ithin 3 dnyj 9 6m m3 m 3 3 m3 m 10 U m m3 m 15 15 m m3 m Par con fc M ortality 19.9 37.5 30.0 ' 50.0 75.0 773808 Tha r e s u lt* a re co n v en ien tly ta b u la te d in ta b la 1 Xifci* ; i i noted, a doaa of Z to S,5 ee. par fcllogras ia t a 75 par a an t a o r ta llty * tre a ty -tw o o r t h i s croup of vara autopslad* Another group of 0 ra ta v at given s in g le o ra l doses ranging fro x 113 to l. 0 ee* p ar kllogran* fils o f th e se v ara k i l l e d Z days a t t a r t r a a t s a n t and e s s s ln e d by th* pathology d a p a rtsa a t f o r p o asib la l i v e r Manage Six v ara Id J l- 8 days a f t t r tra a ts a n t and axanlned fo r p o sslb la kidney change and 6 vara k ille d 11 days a f ta r tr a a ts a n t, and exaslned fo r p o s s lb la b ra in damage .. (fi) Baoaatad P o s ts *v * * T h irty r a t s v ara dlvldad in to groups of tan Tha f i r s t group raealT ad fro x 0*35 to 0*56 cc* per k ilo g r a s f o r 5 t r a a ts a a t s * Iona d ie d and 5 v ara k i l l e d f o r au to p sy w i t h i n 24 hours a fte r tha 3rd tra a ts a n t I s tha second group, th a do sag ranged fro x 0*63 to 0*88 cc* p a r k llo g r a s * Two d ie d a f t e r th e second tra a ts a n t and 8 v ara k ille d f o r autopsy 4 hours a f t e r tha l a s t traataa& t In tha th ird group tha dosage ranged fre s 1 .2 3 to 1*78 cc p e r k ilo One d ied a f t e r 1 t r a a t s a n t , 3 dlec a fte r trs a ts e n ts , and 4 died a fte r the 3rd tre a ta e n t* A ll a n ls a ls In th is l a s t group v ere au to p sle d w ith in 4 h r s . o f t l la s t treatsent In"general a l l r a ts behaved the sane follow ing oral dalnlstration o f la a rte a n They proceeded a t once to e a t as I f to a l l a y the d is c o m fo rt caused by th e su b sta n c e * f o l l o v i n ; t h i s th ey became d e p re s s e d and v o u ld n o t eat. and o f t e n w ould : GENP 010195 drlafc* ts c ta s s * stupor generally iup#ntn#d n th lA i fv hour* u n t i l tb s e n ta s is could do le a s e r r i t e Tor food a&d n tsr* Liquids were taken, however, i f adm inistrated tad fo r A frv minutes follow ing adm inistration tie animals ported up a b i t . Tbs improvement n s only' temporary tad the outeome, boa th ey bod th u s f o r p ro g re ssed , was in v a ria b ly f a t a l , t e e a o ia a l showed aa e lb u a a n u ria ( te s te d p o s t mortem) and one da eloped tr o v e r s o f tb e h r A, ta d f r o n t limbs* One had aoTere hemorrhages from th e re c tu a and the autopsy in d icated the b leed in g was f r o a th e low er end o f th e e s e l l bowel. Comparison o f X nerttcn w ith Trichlorobenxsne and _ g th y l-te tra -p ente-chlorobeassne A group o f 10 anin& ls was g iv en re p eated d o ses o f trie h lo ro b e n x e n e ra n g in g f r o a 0.56 to 0 .5 8 ee* p e r kilogram * Unt d ied a f t e r th e 1 s t tre a tm e n t, 4 d ied a f t e r th e fend, and 4 d ie d a f t e r th e 3 r d . The r s e in in g anim al was h i l l e d 4 days a f t e r the 3rd and l a s t trs a ta a n t* i l l animals were a u to p a lc d . . S im ila r groups were tr e a te d w ith X nerteen end e th y l te tr a - p e n t chlorobensene and the follow ing noted! D ally d o ses o f 1.23 to 1 ,7 6 cc* per k ilo g ram o f X n erteen k i l l e d 80 p e r c a n t o f th e a n im a ls, when 3 d o se s w ere g iv e n , w h ile d u lly , d o ses o f 0.36 to 0 .5 5 cc* per k ilo g ra m o f trie h lo ro b e n x e n e k i l l e d 90 p e r c e n t o f th e anim als w ith 3 do a; GENP 010196 0a the other hand d a lly doses of eth y l-tetra-p en ta-ch lo ro b en x : ranging from 0.62 to 1*04 ec, p er k ilo g ram * k illed 1 r a t in a group of 10 w ith Z doses* C o n clu sio n ! 773809 The minimum f a t a l doi-e o f X nerteen eppears to be 4 S.O oc. p ar W l ^ r u . A m of la irtic n n ? i ftt& l l a concen trations between 0.96 iad l7ft te* ^r k ilo s? * , tilla r p tttd doses of trichlorobensane vara f a ta l ln ooneea* ^ r a tio n s between 0*36. and C*9B aa* p a r kH agren. X thylt e t r a p ^ n ta-eh lo reb e aseae doaa n e t ap ro ar to toa n a a rly so to x le ky oral id a la lstn tlo o . fia lf a o u b lc c e n tim e te r o f la a r ta a n was a p p lie d d a l. to th e shaved backs o f 10 r a t a by means o f a sm all s la a a cup f I l i a d w ith a p i a c t o f e o tto n wool aoakad l a th a aubs t e n e t . T. whole was atra p p ad t o th a anim4 als w ith ad h esiv e tap One r a t dlad a f te r 11 tre a tm e n ts . Three d ie d a f te r 13 tr a a ta a n ta and r * c a ln in s anim al v a ra k i l l e d f o r au to pay 34 h o u rs a f t e r ta e 1 tre a tm e n t. i l l r a ta l o s t w eight d u rin g tha experim ent, b u t ou side of a a lls h t raatlaaan aas a t tha beginning of tha experlns shoved no o th e r s ig n s . Tha Z n ertean a c te d l o c a l l y on th e ak in producing an excessive dryness w ith subsequent th ick en in g and sc a lin g v itn o u t any fis s u r e s being formed* th e sane a f fe c ts > noted on tha human s k in v i t h ra a p a c t .to th a d ry in g and s l i g h t sc a lin g and vare c o a t n o ta b le on th a hands, cheeks and foraha* inhalation . i n a tte m p t was cade to re p ro d u c e c o n d itio n s s o re sc than would be n e t l n th a o v e rh e a tin g o f a tra n s fo rm e r and expc lng r a t s to th e s e c o n d itio n s f o r 0 c ln u te s t v l c t a d ay . GENP 010197 I- 773811 Sh< T o ru o la c i i u r u n p r u i a t i a box of i b o t t 1 cubic fo o t c a p a c ity , i s one corner o f which i s the iA l t r?oc a l l u s fle sh containing the subetenet being in v e stig a te d . 2a- r t i i ^ t i m I e r t r t a s ^ a t r 11 (W astlnghouse) vers plaeed i s the f le s h sad brought to e b e ll* two r e t s ware them placed Im th e box end allow ed to remain f o r to m in u te s, d u rin g which time e g s n tra lix e d itc h in g o f th e s h in eppeered which wes most merhed in the p e r ts o f the body n o t p ro te c te d by h e ir# th e itc h in s is p e r s is te d f o r sometime e f t e r th e e n la s la were remore from th e box* PyTamol seemed to produce th e c o s t e f f e c t In th is re sp e c t w ith In e rts s n end Transform er o i l follow ing In th o rd e r n aaed . T hree o u t o f 6 o f th e X n erteen t r e a t e d r e t s l o s 4> w eight, w hile the f j r t n o l end T ren sfo raer o i l tre e te d r e t s gei w eight ( r e t s only in eech o f the l e s t two groups) I t i s q u ite possible th a t these animals in g ested e considerable asoi o f th e se su b sta n c e s by lic h ln g t h e i r f u r a f t e r reaoT& l from t: X box. ho merhed change In r e s p ir a tio n s were n o te d , a lth o u g h t en isels once or tw ice breathed f e s te r than normal and occasic a lly th e e x tre m itie s were s lig h tly b lu e . Xt autopsy no gross pathology-w as n o te d . The maximum c o n c e n tr a tio n o f X n e rte e n t which th e s e r a t s were exposed was ab o u t 0 .5 mg. p e r l i t e r . T box was a ir e d betw*een r e a d in g s , b u t n o t washed o u t. With I n e r t an two r a t s r e c e iv e d ZB tr e a tm e n ts , tc ` re c e iv e d 35 tre a tm e n ts end two re c e iv e d 66 tr e a tm e n ts . With P y ran o l two r a t s re c e iv e d 42 tre a tm e n ts and w ith T ran sfo rm er O il tvo r a ts re c e iv e d 44 tre a tm e n ts. GENP 010198 -C- 773812 X p o u p o f U a r a t s was ozpoiod fo r to 4471, i i ^ t our* par day to o o n aan tratio n s of Z aerteen l a the a i r ro g in g fro 0.00 to 0.09 k . par l i t e r , i l l r a ts * n t i ll e d fo r pa to o l* CJ w ith in tv o days fo llo w in g th a COth days of treatm en t -Sight of th is croup gained w eight, oaa lo s t weight tad oaa ehovad ao change* J iv e shoved a s lig h t drop la the rod blood eo u n t, th rs e showed aa la e ra a sa d rod blood aount aad tvo show: ao change* The changes l a th e b e a o g lc tia p ar a l l s l t d th e ehan: in the rad blood eount* A ir a n a ly s is f o r E alo v an ttad H rd ro ra n ita d Hydr o c a r b o n . C onsldarabla d if f i c u l t y was axparlancad la obtain!: a s a t i s f a c t o r y method o f a i r a n a ly s is f o r th a se su b stan ce s an* a n e p h e lo m e tric s e th o d vas f i n a l l y adopted* Za t h i s , a d s f i a oluae o f th e a i r to ba ta s ta d uas drawn a t a r a ta o f about 1 11 t a r every fo u r m inutes through 5 c c . o f a th y l a lc o h o l c o o lt in a dry ic e -a c e to n e a ix tu re * The l a t t e r being n ec assary to * a p re v e n t e v a p o ra tio n o f th a alco h o l* Oaa cu b ic c a n ttw a te r of th a a lc o h o l was th a n mixed w ith 13 c c . o f d i s t i l l e d w ate r an: re s u lta n t tu rb id ity , measured w ith a photo a la c tr ic c a ll , u si: a so d lu s vapour lamp as a source o f lig h t* Tha t u r b i d i ty of inown s t a p l e s waa compared to th a t u r b i d i t y produced by Jcaovr s ta n d a rd s cade up o f trie h lo ro b e n s e n e and e th y l a lc o h o l, Th: method gave a s a tis f a c to r y rang o f re a d in g s fo r c o n c e n tra te v a ry in g .fro c 0 ,2 0 ngl p ar l i t e r - t o 0*03 ng*# p er l i t e r which, when th e o r i g i n a l d i l u t i o n s w ars taVcen i n t o acco u n t, was su f c ia n t to d e te c t as low as 0*03 ag* p er l i t e r o f Z nertaen in a i r te s te d . The method i s n o t s p e c if ic f o r th e v ario u s cen t ENP 010199 7 773813 Ttflem D litiae. f?e= l o w of Affect ef varying the distance At feien aaaple* er Uicta fron a e lsit space o rtr boiling laerteaa as detsr alaed with the following rotultss Plstanoo Inahwe C o aeitratloa 14 4.50 I0f i . i e M* 1.14 i.i4 : lln lla r ly the *ffct of varying the distance a t which saa p le s w art taken over an open beaker o f b o ilin g In e rt was d e te r s ln e d w ith th e fo llo w in g r e s u l t s i W.stan ce C oncentration e r . 'ner l i t e r S 0.56 ! 10 0.41 15 O.fcd 10 0.14 Cio c o n c e n tra tio n s f a l l o f f r a p id ly and I f any eo< lag surface Is anore the In e rte e n and no a ir c u rre n ts are bit in s o r e r th e s u r f a c e o f - th e In e rte e n * th e r e i s much l e s s cha: of any a p p re c ia b le aaount escaping In to th e atnosphere as fr- a container lik e *tr ansforser# 6*gpU ag a t V arloui r i s e I n te r v a ls and AtJLir 13 l l t t r s p e r n ia u te was p a sse d o v er a oq.su rfa ce o f I n e r te o a (ab o u t 10 Inches) contained In a f la G E N F 010200 a 7?3B U ao a o GENP 010201 j a r i t v ario u s ln ta r r a ls a f te r the a ir f i n vas s ta rte d and under various Tin# o f S ta p lin g of temperature* Coneoatratloc of T ri ohlorobensaae Temperature of T rlohlorobense& e e. At u*e. 1 /1 "hour s s 0*19 0,31 0,60 S3 S3 13 ' M 60C. At 90C. 1 4 i 1.8* 1.70 1.90 SO so so Znertean a t M C . 6 o ,u 13 from th e foregoing i t w ill be no ted th a t th e 6 ho s ta p le v i t h t r 1 c h i oro bans ene a t 23C* e lv e s alm o st th e sane cen tratio n (0*60 as* per l i t e r ) as did the s ix hour s&sple * X aerteeri a t 3c C, (0*58 me* p e r *11ta r ) T h is su g g e sts th e p b ility th a t th e bulb o f the s tu f f coning o ff the Znerteen at th a t te s p e ra tu re aay he trlch lo ro h en x en e and th a t the haxarc I n e r te ea say th u s he th e h a sa rd s o f trlc h lo ro h e n x e n e * T racr e l d i s t i l l a t i o n o f about 338 c c , o f I n e r t en vas c a r r i e d ou* a d i s t i l l i n g f l a s k w ith a 300C* the m on e t e r re a c h in g to th lev el of the side a n . the d is tillin g fla s k vas' attached t: Liebig condenser and a co lle c tin e : flask * Samples v ere c o ll over te s p e r a tu re ranges fro& 40C. to 300C* (The te n p e ra t' recorded being t h a t o f th e vapors around th e th e rn o n e te r bu The d is ta n c e betw een th e b u lb and th e s u r f a c e o f th e l i q u i d about 6 Inches* Although the liq u id b o iled rig o ro u sly sere 773B15 lax p o in t and s p e c ific p a r i t y m liiiu r a d fo r n c n fra c tio n c o lle c te d . Temperature of lo llin g Point F ra e tlo a Condensing Liquid P raatio a SO. c . 89*0 Crav. T e l. 1 40 - 08-119 1 .4 .9 38 40-154 818 1.478 45 3 54-260 40 1.493 43 4 80*300 89 1.577 50 Residua .Above 300 1.598 ISO T r a c tio n #1 n i probably tr ic h lo r o b c n tc n c (8 p e c i- t i e g r a v ity 1 .4 6 6 , b o ilin g p o in t 08 - 1 9 C .). C oncluglona and In in v e stig a tin g the toxicology of In crteen answer to th e `fo llo w in g c r e a tio n s ware sought and a ra h ere g iv en as far as p o ssio lt. ( 1 ) . What i s th e c o n p o sitlo n o f th e Tapors l i b a r : fro n In c rte e n a t a naximus vow ing ten p aratu ra of 93C ., Iriehlorobenxent only. () t t i a t i s th e maximum c o n c e n tra tio n o f t h i s fra c tio n to rh ic h vorL ers n ig h t possibly ba exposed, (a) Za a clo sed space 14" over b o ilin g In e r 4 .5 &( p er l i t e r . #(b) I n an opan space 5* o r e r b o ilin g I n e r t e 0.36 ng. per l i t e r . ( 3 ) . t h a t m ight be th e maximum c o n c e n tra tio n i n roos th ere transform ers ara placed hen the tenparatura of 1 GENP 010202 10 _ *krg 1*1 ag. par U t i r i ( i ) # th a t Is a aa f t ooocantration as ahem k j axpsrlm ssts Consldsrlng tbs hazard Is doa, under erd in ary working conditions to trle h lo ro b a a ic a e alone, (a ) from trlehlorobanseae data previously o b ta in e d , 033 me* p a r l i t e r ( b ) P ro s I n a r te e n d a ta - 048 ag p er l i t a S ^ i l a s t f ig u r e has bsaa v t r i f i a d by ezp o slag r a t a fo r 6 hours par day to sueh eo aeaatratlo n s over a p erio d of '(5) Xs th a ra a sA spla t a s t t h a t a l i i d a t a c t con c e n tr a tio n s o f X nartaan below *048 mg* p a r l i t e r * la s* I f 10 l i t e r s o f a ir ora d ram a t a r a ta of about ona l i t e r every s in u te s through 1 cc o f e th y l a lc o h o l (cooled in dry le a o r an le a s a l t m ix tu re) and the alco h o l poured over the au rfaca of a few cc. of d is tille d w ater in a te s t tube a rin g o f tu r b id ity w i l l be produced i f th e concentration is over 0.03 sc* per l i t e r . If a rin g Is produced, the a ir ta ste d shoul be regarded w ith s u s p ic io n and in c re a se d v e n tila tio n provided. ( 6 ) . t h a t i s th e minimum f a t a l d o te c r a to 3*5 c c . per kilogram (7 ) . Is there any shin hazard? Constant ezpcsu GENP 010203 U 7 w4r y tn s a i xpoi*6 to tto it t u m u m U eb B Koaa Xa u l i i ia B U iilT t ljr irr Bkl&s, Z/U /S8 4* rc GENP 010204 773817