Document 9J4KLagpXg5QX35oydB183DQ6
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Wildlife International, Ltd.__________________ __________ Project Number 454A-104
PFOS: A 48-HOUR STATIC ACUTE TOXICITY TEST WITH THE CLADOCERAN (Daphnia magnet)
ADDENDUM
WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-104 3M LAB REQUEST NO. U2723
U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines
OPPTS Number 850.1010 and
OECD Guideline 202
This study was reported using a test substance purity o f 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratio of the two purities or a factor of 0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data. As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate of analysis.
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Wildlife International. Ltd.
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Project Number 454A -104
REVISED STUDY SUMMARY BASED ON A PURITY OF 86
SPONSOR:
3M Corporation
SPONSOR'S REPPRESENTATtVE:
Ms. Susan A. Beach
LOCATION OF STUDY, RAW
DATA AND A COPY OF THE
Wildlife International Ltd
FINAL REPORT: ---------- ---------------- -- - ......................................................... --I-TM - -
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WILDI 1IT INTERNATIONA1
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LTD. PROJEt 1 Nl MBER
4S4A-104
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TEST SUBSTANCE:
PFOS (Perfluorooctane Sulfonic Acid Potassium Salt)
STUDY:
PFQS: A 48-Hour Static Acute Toxicity Test with the Cladoceran (Daphnia magna)
MEAN MEASURED TEST CONCENTRATIONS:
Negative Control, 11, 19, 32, 54 and 87 mg a.i./L
TEST DATES:
Experimental Start - February 16,1999 Biological Termination - February 18, 1999 Experimental Termination - February 18,1999
LENGTH OF TEST:
48 Hours
TEST ORGANISM: SOURCEOF TESTORGANISMS:
AGEOF TESTORGANISMS:
Neonate cladocerans (D aphnia m agna)
Wildlife International Ltd. Cultures Easton, Maryland 21601
< 24 hours at test initiation
48-HOUR EC50:
95% CONFIDENCELIMITS:
NO MORTALITY/IMMOBILITY CONCENTRATION:
NO-OBSERVED-EFFECTCONCENTRAHON:
59m ga.i./L 32 and 87 mg a.i./L 32 mg a.i./L ;i':3 f !8 a.i./L
Wildlife International, Ltd.
-3 CERTIFICATE OF ANALYSIS
Project Number 454A-104
INTERIM CERTIFICATE OF ANALYSIS
Revision 1(9/7/00)
Centre Analytical Laboratories COA Reference #; Q23-018A
3M Product; P F O S,L ot217
Reference #: SD-018
___________ __________ Purity; 86.9%___________ _
Test Name
Specifications
Result
Purity1
Appearance Identification
NMR Metals (ICP/MS)
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White Crystalline Powder
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86.9% Conforms
Positive
1. Calcium 2. Magnesium 3. Sodium 4. Potassium2 5. Nickel 6. Iron 7. Manganese
1. 0.005 wt./wt.%
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2. 0.001 wt./wt.%
3. 1.439 wt./wt.%
4. 6.849 wt./wt.%
5. <0.001 wt./wt.%
6. 0.005 wt./wt.%Si%5;
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7. <0.001 wt./wt.%
Total % Impurity (NMR)
:
1.93 wt./wt.%
Total % Impurity (LC/MS) Total % Impurity
8.41 wt./wt.%
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None Detected
(GC/MS) Related Compounds POAA Residual Solvents (TGA)
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0.33 wt./wt.% None Detected
Purity by DSC Inorganic Anions (IC)
1. Chloride 2. Fluoride 3. Bromide 4. Nitrate 5. Nitrite 6. Phosphate 7. Sulfate4
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Not Applicable3
1. <0.015 wt./wt.% 2. 0.59 wt./wt.% 3. <0.040 wt./wt.% 4. <0.009 wt./wt.% 5. <0.006 wt./wt.% 6. <0.007 wt./wt.% 7. 8.76 wt./wt.%
Organic Acids 5 (IC) 1. TFA 2. PFPA 3. HFBA 4. NFPA
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1. <0.1 wt./wt.% 2. <0.1wt./wt.% 3. 0.10wt./wt.% 4. 0.28 wt./wt.%
Elemental Analysis6: 1. Carbon 2. Hydrogen 3. Nitrogen 4. Sulfur 5. Fluorine
1. Theoretical Value = 17.8% 2. Theoretical Value = 0% 3. Theoretical Value = 0% 4. Theoretical Value = 5.95% 5. Theoretical Value = 60%
1. 12.48 wt/wt.% 2. 0.244 wt./wt.% 3. 1.74wt./wt.% 4. 8.84 wt./wt.% 5. 54.1wt./wt.%
COA023-018A
Page 1 o f 3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-018A
Date o f Last Analysis: 08/31/00
Expiration Date: 08/31/01
Storage Conditions: Frozen D-10C
Re-assessment Date: 08/31 /0 1
`Purity = 100% - (sum o f metal impurities, 1.45% +LC/MS impurities, 8.41%+Inorganic Fluoride, 0.59%+NMR impurities, 1.93%+organic add impurities, 0.38%+POAA, 0.33%)
Total impurity from all tests = 13.09% Purity * 100% - 13.09% = 86.9%
2Potassium is expected in this salt form and is therefore not considered an impurity.
3Purity by DSC is generally not applicable to materials o f low purity. N o endotherm was observed for this sample.
4Sulfur in the sample appears to be converted to SO4 and hence detected using the
inorganic anion method conditions. The anion result agrees well with the sulfur
determination in the elemental analysis, lending confidence to this interpretation. Based
on the results, the SO4 is not considered an impurity.5
5TFA HFBA NFPA PFPA
Trifluoroacetic acid Heptafluorobutyric add Nonofluoropentanoic acid Pentafluoropropanoic add
th eo retica l value calculations based on the empirical formula, CsFi7S03Tt+(MW=538)
This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160).
COA023-018A
Page 2 of 3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-018A
LC/MS Purity Profile:
Impurity C4 C5 C6 C7
Total
vrt/w t % 1.22 1.33 4.72 1.14 8.41
Note: The C4 and C6 values were calculated using the C4 and C6 standard calibration curves, respectively. The C5 value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves.
Prepared By: ________________________________
____
David S. Bell
Date
Scientist, Centre Analytical Laboratories
Reviewed B y : ________________________________
____
John Flaherty
Date
Laboratory Manager, Centre Analytical Laboratories
COA023-018A
Page 3 of 3
,ildlife International Ltd.
ECO TO XICO LO GY & ANALYTICAL TESTING SERVICES
September 27,2000
Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue 3M Building 2-3E-09 P.O. Box 33331 St. Paul, Minnesota 55133
Dear Susan:
Enclosed please find Addendums for Wildlife International, Ltd. Project Numbers 454A-101, 454A-102,454A-103A, 54A-104, 454A-105,454A-106,454A-107,454A-108 and454A-109
If you have any questions, please let me know.
KRD/cyw8/3m Enclosures
Kurt R. Drottar Senior Biologist
8598 Com m erce D rive Easton, Maryland 21601 Tel. 410-822-8600 Fax 4 10-822-0632 E-mail ecotox@ wildlifeinternational.com
European Office: Overscheenseweg IIA 1412 A A Naarden -The Netherlands -Tel.31(0)356949261 Fax 31(0)356948229 E-mail aleopold@worldonllne.nl
