Document 9J2BnXELMwOEZLDRQ93LEYQ7V

AR 226 -- 0216 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesuifonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not given. The test sample is FC-206CE (5/23/91). It'spuritywas not sufficiently characterized, though current information indicates it is a mixtureof0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylpheny! ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. Thefollowing summaryapplies to a mixture with incompletely characterized concentrationsof impurities. Datamaynot accurately reflect the toxicityofthe fluiorochemical componentofthe test sample. METHOD: Method: `OECD Method 202 Type: Acute static GLP: No `Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures. Analytical monitoring: Temperature, pH, conductivity, and DO Exposure period: 48-hours Test organism age: <24 hours old Statistical method: Not applicable. Survival was greater than 50% at all test concentrations. Test conditions: Dilution water: Filtered natural well water Dilution water chemistry: Conductivity: 860 pmhos/cm pH: 76 Lighting: A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provideda light intensity of 20 uEs'm?2 Stock andtestsolution preparation: A 1000 mg/L primary stock solution was prepared in dilution water. Aliquots were then added directly to dilution `water in test vessels to create test solutions. - Exposure vessels: 250 mL Pyrex glass beakers containing 200 mLoftest solution and a water depth of ~6 cm. Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: Five plus a blank control 001785 Water chemistry during the study: Conductivity range (0-48 hours): 860 --880 pmhos/em (control exposure) 860 -- 880 pmhos/om (1000 mg/L exposure) pH ran7g.e6 (048 -8.3 hours): (control exposure) Temper7a.t7u-r8e.3ra(n1g0e00(0m-g4/8Lhoeuxrpso)s:ure2)0 - 21C 1199.0.0 ---- 2200..12 CC ((c1o0n0t0romlge/xLpoesxuproes)ure) Dissolved oxygen range (0-48 hours): 88..75-99..23 mmgg//LL ((c1o0n0tr0omlge/xLpoesxuproes)ure) RESULTS Nominal concentrations: Bk control, 150, 250, 400, 600, and 1000 mg/L Element values: 48-hour ECso = >1,000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test `Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS The FC-206CE 48-hour ECso was determined to be >1000 mgiL, the highest concentration tested. No mortality or sublethal effects were observed at this `concentration. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES or Test was conducted by EnviroSystems Division or Resource Analyst, Incorporated, Hampton, NH at the requestofthe 3M Company, St. Paul, MN, Lab Request number J1884-2, 1991. 001786 OTHER Last changed: 6/26/00 001787 me a RETA ONE > 3 " i " BEST COPYAVAILABLE ne Sate hatfrrotaoe otf r-escce = iii of HaaaAunntho5r.se.wet i 3 fh code fae cuopen hale 5 BoC ce res mate Leia finial na #= oC nec C|hiSi a. 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