Document 99xd4r7pb2wY2q2ZKEVmOwBEp

A R a .a -6 - 0 9 \ & ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3103. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FX-3103, 3%. Current information indicates it is a mixture of 0.96% PFOS, 20% diethylene glycol butyl ether, 50.56% water, 15% ethylene glycol, 4.32% sodium octyl sulfate, 6.0% polyoxyethylene monooctylphenyl ether, 2.5% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ M ethod: Not given. Type: Static acute GLP: No Y ear com pleted: 1985 Species: Daphniamagna Supplier: In-house cultures. Originally obtained from U.S. EPA-ERL, Duluth, MN. A nalytical m onitoring: Temperature, pH, and dissolved oxygen. Exposure period: 48-hours T est organism age: <24 hours old Statistical m ethods: EC50 values calculated using logistic regression methods (probit analysis). Test conditions: Dilution w ater: Carbon-filtered well water. Dilution w ater chem istry: Not given. Lighting: Not given. Stock and test solution preparation: A primary stock solution was prepared by dissolving 1000 mg of test substance in 500 mL water. Exposure concentrations were prepared by addition of stock solution to each vessel. Exposure vessels: 250 mL glass beakers containing 200 mL exposure " solution at a depth of 6 cm. Num ber o f replicates: 2 Num ber o f daphnids per replicate: 10 Num ber o f concentrations: five plus a blank control W ater chem istry during the study: 002969 pH range (0-48 hours): 8.1 - 8.2 (control exposure) 7.8 - 8.1 (1000 mg/L exposure) Tem perature range (0-48 hours): 22 C Dissolved oxygen range (0-48 hours): 8.9 - 9.3 mg/L (control exposure) 7.0 - 9.3 mg/L (1000 mg/L exposure) R E S U L T S ________________________________________________________ Nom inal concentrations: Blank control, 10 0,1 80, 320, 560, and 1000 mg/L. Elem ent values: 48-hour EC50 - >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance rRmarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance 48-hour EC50 was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY__________________________________ _________________ Reliability: Klimisch ranking = 3. Study lacks description and records of method followed. The study lacks information on purity of the test substance and analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C 1111-1,1985. OTHER__________________________________________________ Last changed: 6/26/00. 002970 ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET LR / V / / / - / TEST CHEMICAL TESTED LOT___________ TEST ORGANISM 7 ? ^ ^ SOURCE/ ^ y > / - ^ ^ 2------ DATE RECTI___ / ^ A / AGE OF TEST ORGANISMS:<24 hrs. old instars (neonates) NO. ORGANISMS PER TREATMENT TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml DEf^R 6cm TIME STARTFQ HATE STARTED ___DATE C O M P L E T E C U ^ ^ ^ S Z DILUTION MATER C arbon-filtered wep water (Temp.^ ^ ; C. D.O. ^ ppm. pH 'ft, / ) ANALYS^ ^ t ^ ^ / ^ g ^ ^ ^ V ^ a g ^ x EC^0 ( 95% Confidence Lim its)_________________________ TEST I n i t i a l T o ta l VESSEL Gone. Amount NO. mg/1 Added -* ytU1 X - Cbntro] 24 Hours T o ta l No. pH Dead o 48 Hours D.O. T o ta l No. PH D.O. ppn Dead ppn * _ ? tf,? | - | lo o \o n n \~?< 1P )0 I n n SV 1-- =s X 2D & Z j o o \l ft? 1-4 SnO |-^ \ n m ID O <? <-> n m fiO m H BEST COPY AVAILABLE Test Tene. qC1 I n i t i a l s & Date COMMENTS : w y/r^ ' -rOAf.fos' fiu ifs t/' ''ra & j, r ? r ^ y / K ' Z St/&/ /A />( / ( Y r / s'f r ? / ^ r e t ir e / ___________________________ 002971 ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET lr/ 7 / # v -- / TEST type TEST ORGANIS fiw rtSnSnfl chemical tested Y - , V S O U R C E ^ ^ ^ ^ ^ jL -DATE R E C O . lot *- 81./ AGE OF TEST ORGANISMS:-C24 h r s . o l d I n s t a r s (n e o n a te s ) NO. ORGANISMS PER TREATMENT / f l TEST VESSEL: 250m l P y re x g la s s b e a k e r TEST SOLUTION VOLUME: 200m l ISe p S F: 6cm TIME STARTED DATE STARTED DATE CDMP1 FTEn D I LUTION MATER C a r b o n - f i l t e r e d w e ll w a t e r (Tem p. J $ L 2 C . D . O . ^ J 5^ ppm. pH P i, / ) (95% C o n fid e n c e L i m i t s ] _________________________________ TEST Initial Total 1 24 Hours VESSEL Gone. Amount Total No. PH NO. mg/1 Added Dead T T - Contro: .0 48 Hours | D.O. Total No. pH D.O. PiTM Dead cCTc*m" *. &2 2 2 - | loo o ' o %2 rZ i m c=53Q tfi o p ` o ft? # , < P 34. 2 5 S^lnC) p o =? & 2 loon inn & 2 2(2, OD Cm/) O 0 u < 1 E OD m COMMENTS: Test Ten. C 23 _Initials & Date JU '-- r g s r a c y i r tf/ & M * d i. s /fa /iz 'M r Ll SECONDARY .TEST VESSEL , lin Symptoms or Reactions Control T0M eER iFw ' . 002972 ENVIRONMENTAL LABORATORY L --/ TABLE I. SUMMARY OF ACUTE T O XICITY OF TO DAPHNIA MAGNA UNDE r V //i EXPOSURE CONDITIONS*1* V CHEMICAL CONCENTRATION (g/l) 24-HR. PERCENT MORTALITY 48-HR. CONTROL ,1Q.Q___ 1P.O ____ .^leQ__ 1PQQ__ o o o o 1 o o o o o 45 EC,.ft (95Z C O N F I D E N C E L I M I T S ) * 2 * V 50 ag/1 > OOO > lo o o 029 (1) '~{2) Data were averages of two replications for each concentration with ten (10) daphnlds per replicate. A total of twenty daphnlds were used per concentration. 1 C lvalues were calculated using logistic regression aethods (Problt Analysis).