Document 99p82wQe1Yx44mxa50RXxwMDV

Company Sanitized. Does not contain TS C A CBI. P-1 [ ]: TRANSFORMATION POTENTIAL IN AEROBIC AND ANAEROBIC SOILS WILDLIFE INTERNATIONAL, LTD. PROJECT NO.: 112E-108 Organisation for Economic Cooperation and Development OECD Guideline 307 AUTHORS: Edward C, Schaefer Joel Stenzel Raymond L. Van Hoven STUDY INITIATION DATE: March 10, 2005 STUDY COMPLETION DATE: March 17,2008 Submitted to: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 USA Wildlife International, Ltd. 8598 Commerce Drive Easton, Maryland 21601 USA 1 (410) 822-8600 Page 1 of 298 P-2 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 2- GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT SPONSOR: E.I. du Pont de Nemours and Company TITLE: " ^ Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROIECT NO.: 112E-108 STUDY COMPLETION DATE: March 17,2008 This study was conducted in compliance with Good Laboratory Practice Standards as published by the U.S. Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17), with the following exceptions: Periodic analyses of well water for potential contaminants were performed using a certified laboratory and standard U.S. Environmental Protection Agency analytical methods. The characterization of the test substance, and the stability of the test substance under the conditions of storage at the test site, were not determined in accordance with Good Laboratory Practice Standards. Original documentation for the preparation of two combined stocks were not maintained. Documentation of the stocks preparation was re-created from the information on the stock label. STUDY DIRECTOR: **6 0. Edward C. Schaefer ^ Director of Biodegradation 17 W /U cff DATE P-3 Company Sanitized. Does not contain TSC A CBI. Wildlife International, Ltd. Project No.: 112E-108 -3- QUALITY ASSURANCE STATEMENT This study was examined fox compliance with Good Laboratory Practice Standards as published by the U.S. Environmental Protection Agency in 40 CFR Part 792 and OECD Principles of Good Laboratory Practice (ENV/MC/CHEM (98) 17). The dates of all inspections and audits and the dates that any findings were reported to the Study Director and Laboratory Management were as follows: ACTIVITY: DATE CONDUCTED: Protocol March 1,2005 Stock Solution Preparation March 15,2005 Headspace Sampling March 24,2005 Stock Soludon Preparation April 19,2005 Analytical Data - Day 0 Analyses October 10-26,2005 Analytical Data - Week 1 Analyses October 10-28,2005 Analytical Data - Week 2 Analyses October 10Novembcr 1,2005 Analytical Data - Month 1 Analyses October 10November 1,2005 Analytical Data - Month 2 Analyses October 10November 1,2005 Analytical Data - Month 4 October 10- Analyses November 2,2005 Soil Moisture Determination and Dosing Data October 10December 2,2005 Oxygen Measurement October 17,2005 Dosing Viability Controls October 17,2005 Microbial Biomass Sample Processing . October 18,2005 DATE REPORTED TO: STUDY DIRECTOR: MANAGEMENT: March 1,2005 March 11,2005 March 15,2005 March 16,2005 March 24,2005 April 13, 2005 April 19,2005 April 22,2005 October 26,2005 November 8,2005 October 28,2005 November 21,2005 November 1,2005 November 21,2005 November 1,2005 November 21,2005 November 1, 2005 November 21,2005 November 2,2005 December 1,2005 December 2,2005 October 17,2005 October 17,2005 August 30, 2006 October 19,2005 September 26, 2006 October 18,2005 November 3, 2006 P-4 Company Sanitized. Does not contain TSC A CBI. Wildlife International, Ltd. Project No.: 112E-108 ACTIVITY: Analytical Sample Processing (6-month) Month 6 Analytical Data Analytical Draft Report 9 and 12 Month Analytical Data and Tables Biological Data and Draft Report Data and Draft Report (including IS, 18 and 24 month analytical data) Final Report -4- QUALITY ASSURANCE STATEMENT (continued) DATE CONDUCTED: DATE REPORTED TO: STUDY DIRECTOR: MANAGEMENT: October 20,2005 November 14-17,2005 November 17-22,2005 August 21-25 and 28, 2006 October 23 November 9,2006 October 24 November 2, 2007 October 20, 2005 November 17, 2005 November 22, 2005 August 29, 2006 November 9,2006 November 2, 2007 October 24,2005 November 28,2005 December 1,2005 October 3, 2006 December 18, 2006 November 20,2007 March 17,2008 March 17,2008 March 17,2008 All inspections were study-based unless otherwise noted. Linda R. Mitchell Director of Regulatory and Ecotox Operations 17 M/h u j DATE P-5 Company Sanitized. Does not contain TSCA CBI. Wildlife International, Ltd. Project No.: 112E-108 -5 REPORT APPROVAL SPONSOR: E.I. du Pont de Nemours and Company TITLE: Q J Transformation Potential in Aerobic and Anaerobic Soils WILDLIFE INTERNATIONAL, LTD. PROJECT NO. : 112E-108 STUDY DIRECTOR: <% / Edward C. Schagfr Director of Biodegradation /7 DATE 2-oob MANAGEMENT: Director of Aquatic Toxicology/Terrestrial Plants and Insects 3 DATE Willard B. Nixon, Ph.p. Director of Chemistry DATE P-6 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project n 0.: 112E-108 -6- TABLE OF CONTENTS Title Page.......................................................................................................................................................... 1 Good Laboratory Practice Compliance Statement......................................................................................... 2 Quality Assurance Statement.......................................................................................................................... 3 Report Approval............................................................................................................................................... 5 Table of Contents.................................................................... 6 Study Information........................................................................................................................................... 16 Introduction............................................................ 17 Objective........................................................................................................................................................ 17 Experimental Design...................................................................................................................................... 17 Materials and Methods...................................................................................................................................18 Test Substance.......................................................................................................................................... 18 Measured Analytes................................................................................................................................... 18 Internal Standards................................................................................................................................... 21 Test Soils................................................................................................................................................ 22 Test Apparatus and Conditions............................................................................................................. 23 Soil Pre-Incubation................................................................................................................................. 23 Preparation of the Test Chambers......................................................................................................... 23 Maintenance of Test Chambers............................................................................................................. 24 Sampling and Measurements.................................................................................................................25 Quality Criteria....................................................................................................................................... 25 Viability Assessments............................................................................................................................ 26 Results...........................................................................................................................................................26 Test Conditions.......................................................................................................................................26 Characterization of Soils........................................................................................................................ 27 Sample Processing and Analyses...........................................................................................................27 Headspace Analyses........................................................................................................................ 27 Soil Analyses....................................................................................................................................27 Stock and Calibration Standard Preparation................................................................................... 29 Calibration Curves and Limits of Quantitation............................................................................... 31 References 34 P-7 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Projectn0.: 112E-108 -7TABLE OF CONTENTS - Continued - TABLES Table 1. Frequency of Measurements....................................................................................................... 35 Table 2. Test Chamber Headspace Oxygen Content................................................................................36 Table 3. Selected Soil Properties...............................................................................................................37 Table 4. Soil Viability by Glucose Mineralization:Alfisol........................................................................ 38 Table 5. Soil Viability by Glucose Mineralization:Inceptisol...................................................................38 Table 6. Soil Viability by Glucose Mineralization:Ultisol........................................................................39 Table 7. Soil Viability by Glucose Mineralization:Mollisol.................................................................... 39 Table 8. Soil Microbial Biomass...............................................................................................................40 Table 9. Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas.......... 42 Table 10. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts..................................................................... 43 Table 11. Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts....... 44 Table 12. Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts........................................................................................................................................ 45 FIGURES Figure 1. Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers......... 46 Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts...........................................................47 Figure 3. Representative ion chromatogram for 8-2 FTOH in a low-level (25.0 ng a.i./mL) calibration standard by GC/MS...................................................................................................48 p.8 Company Sanitized. Does not contain TSCA CBI. \Yildlife International, Ltd. Project No.: 112E-108 8- TABLE OF CONTENTS - Continued - Figure 4. Representative ion chromatogram for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS.................................................................................................. 49 Figure 5. Representative ion chromatogram for 8-2 FTOH in a low-level (10.0 ng a.i./mL) calibration standard by LC/MS/MS.............................................................................................50 Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mT.) calibration standard by LC/MS/MS.............................................................................................51 Figure 7. Representative ion chromatogram for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS.............................................................................................52 Figure 8. Representative ion chromatogram for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS.............................................................................................53 Figure 9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS............................................................................... 54 Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standards by LC/MS/MS............................................................................... 55 Figure 11. Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................................. 56 Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a,i./mL) combined PFCA calibration standard by LC/MS/MS................................................................................. 57 Figure 13. Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 58 Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 59 Figure 15. Representative ion chromatogram for PFOA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 60 Figure 16. Representative ion chromatogram for PFOA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 61 Figure 17. Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS........................................ 62 P-9 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project no.: 112E-108 -9TABLE OF CONTENTS - Continued - Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 63 Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 64 Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS............................................................... 65 Figure 21. Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by GC/MS .....................................................................66 Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS................................................................................. 67 Figure 23. Representative calibration curve for 8-2 FTOH by GC/M S......................................................68 Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS................................................ 69 Figure 25. Representative calibration curve for 7-2 sFTOH by LC/MS/MS...............................................70 Figure 26. Representative calibration curve for 8-2 FTA by LC/MS/MS...................................................71 Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/MS..................................................72 Figure 28. Representative calibration curve for 8-2 FTUA by LC/MS/MS................................................73 Figure 29. Representative calibration curve for PFOA by LC/M S/M S..................................................... 74 Figure 30. Representative calibration curve for PFNA by LC/MS/MS..................................................... 75 Figure 31. Representative calibration curve for PFDA by LC/MS/MS..................................................... 76 Figure 32. Representative calibration curve for PFUA by LC/MS/MS..................................................... 77 Figure 33. Representative ion chromatogram for 8-2 FTOH in the sampled headspace (Cig cartridge extract) from a Day 0 untreated control soil by GC/MS .......................................................... 78 Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS......................................................................................... 79 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 - 10TABLE OF CONTENTS - Continued - Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS.........................................................................................80 Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.........................................................................................81 Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS.........................................................................................82 Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.........................................................................................83 Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.......................................................................................................... 84 Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS..........................................................................................................85 Figure 41. Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.......................................................................................................... 86 Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS.......................................................................................................... 87 Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8 cartridge extract) from a Month 24 untreated control soil by GC/M S..................................................... 88 Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 89 Figure 45. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 90 Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 91 Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 92 Figure 48. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 93 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -11 TABLE OF CONTENTS - Continued - Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 94 Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 95 Figure 51. Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS......................................................................................... 96 Figure 52. Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS.........................................................................................97 Figure 53. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Q 8cartridge extract) from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS.................................................................................................................98 Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS.............. 99 Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 100 Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 101 Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 102 Figure 58. Representative ion chromatogram for PFNA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 103 Figure 59. Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 104 Figure 60. Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 105 Figure 61. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C|8 cartridge extract) from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS............................................................................................................... 106 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -12TABLE OF CONTENTS - Continued - Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 107 Figure 63. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 108 Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 109 Figure 65. Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 110 Figure 66. Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ I l l Figure 67. Representative ion chromatogram for PFDA in the soil extract from a Month 12 ., fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 112 Figure 68. Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS............ 113 Figure 69 Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C18cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg H-26634 by GC/MS..................... 114 Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kg H-26634 by LC/MS/MS............................................................. 115 Figure 71. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ ]by LC/MS/MS...............................................................116 Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgQ Jby LC/MS/MS...............................................................117 Figure 73. Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg_ Jby LC/MS/MS.............................................................. 118 Figure 74. Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg J by LC/MS/MS............................................................. 119 Figure 75. Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kgl'' J b y LC/MS/MS.............................................................. 120 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 13TABLE OF CONTENTS - Continued - Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg (_ 3 by LC/MS/MS.............................................................. 121 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8cartridge extract) from a Month 12 sterile soil treated with 200 mg/kg 3 ^ GC/MS............... 122 Figure 78. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kg Q Jby LC/MS/MS............................................................. 123 Figure 79. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC 3 by LC/MS/MS.............................................................. 124 Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg^ 3 by LC/MS/MS.............................................................. 125 Figure 81. Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg ^by LC/MS/MS...............................................................126 Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg^ 3 by LC/MS/MS............................................................... 127 Figure 83. Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg ^ by LC/MS/MS................................................................128 Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg }by LC/MS/MS............................................................... 129 Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C|g cartridge extract) from a Day 0 live soil treated with 200 mg/kg by GC/MS........................ 130 Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live soil treated with 200 mg/kg y>y LC/MS/MS............................................................... 131 Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 live soil treated with 200 mg/kg Dby LC/MS/MS................................................................132 Figure 88. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 live soil treated with 200 mg/kg ^by LC/MS/MS................................................................ 133 Figure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 live soil treated with 200 mg/kg J by LC/MS/MS............................................................... 134 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -14TABLE OF CONTENTS - Continued - Figure 90. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 live soil treated with 200 mg/kg . ^by LC/MS/MS.............................................................. 135 Figure 91. Representative ion chromatogram for PFOA in the soil extract from a Day 0 live soil treated with 200 mg/kg [ }by LC/MS/MS.................... 136 Figure 92. Representative ion chromatogram for PFNA in the soil extract from a Day 0 live soil treated with 200 mg/kg[ ^by LC/MS/MS.............................................................. 137 Figure 93. Representative ion chromatogram for PFDA in the soil extract from a Day 0 live soil treated with 200 m g/kg^ "3 by LC/MS/MS............................................................. 138 Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 mg/kg^ ~3by LC/MS/MS.............................................................. 139 Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C|8cartridge extract) from a Month 24 live soil treated with 200 mg/kg( }jy GC/MS.................. 140 Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg [ ^ by LC/MS/MS...............................................................141 Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg J b y LC/MS/MS..............................................................142 Figure 98. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kg^" ' `JyLC/M S/M S............................................................... 143 Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg )>y LC/MS/MS............................................................. 144 Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/kg ) by LC/MS/MS............................................................145 Figure 101. Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil treated with 200 mg/kg Jby LC/MS/MS............................................................ 146 Figure 102. Representative ion chromatogram for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kg Q }by LC/MS/MS............................................................ 147 Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kg jb y LC/MS/MS............................................................148 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 15 - TABLE OF CONTENTS - Continued - Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kg J b y LC/MS/MS.......................................... ................149 APPENDICES Appendix I. Measured Concentration of Selected Analytes (pg/kg Soil Dry Weight).......................150 Appendix II. Measured Concentration of Selected Analytes (pmol/kg Soil Dry Weight).................. 223 Appendix III. Example Calculations.......................................................................................................296 . Appendix IV. Duration of Incubation.....................................................................................................298 p. 16 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -16- STUDY INFORMATION Study Initiation Date: Experimental Start Date: Experimental Termination Date: Study Completion Date: 10 March 2005 11 March 2005 07 September 2007 17 March 2008 Project No.: 112E-108 Study Director: Sponsor: Sponsor Representatives: Study Personnel: Edward C. Schaefer E.I. du Pont de Nemours and Company William Berti and Bogdan Szostek Edward C. Schaefer, B.S., Director of Biodegradation Raymond L. Van Hoven, Ph.D., Scientist, Chemistry Joel I. Stenzel, B.S., Senior Chemist R. Scott Flaggs, B.S., Biologist, Biodegradation Abul Siddiqui, B.S., Scientist, Biodegradation Frank Lezotte, B.S., Senior Chemist, Chemistry David L. Weiss, M.S., Chemist, Chemistry William Schutt, B.S., Chemist, Chemistry John R. Murrell, B.S., Senior Chemist Willard B. Nixon, Ph.D., Director of Chemistry Henry O. Krueger, Ph.D., Director of Aquatic Toxicology/Terrestrial Plants and Insects Molly E. Matthews, Biologist, Biodegradation Jon A. MacGregor, B.S., Scientist Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 17- INTRODUCTION This report describes a laboratory research study conducted to assess aerobic and anaerobic transformation o f Din soil systems. This study was conducted by Wildlife International, Ltd. for E.I. du Pont de Nemours and Company at the Wildlife International, Ltd. research facility in Easton, Maryland. The raw data generated by Wildlife International, Ltd. and the original final report final report will be archived by the study sponsor. A certified copy will be retained under Project Number 112E-108 in the archives located at on the Wildlife International, Ltd. site. OBJECTIVE The objective of the research study was to assess the potential for transformation of the test substance in aerobic and anaerobic soils. EXPERIMENTAL DESIGN The test was conducted with four test soils under aerobic conditions. The study was initially designed to set-up to also include four soils under anaerobic conditions, however a check of the system indicated that the test chamber closures did not exclude air. The systems initially started under anaerobic conditions up to and including month 6 are included in this report because they were extracted and analyzed as planned. Four groups were established for each soil type and condition (aerobic or anaerobic): (1) untreated (control) live soil; (2) 200 mg test substance/kg dw treated live soil; (3) 200 mg test substance/kg dw treated sterile soil; (4) 250 pg 8-2 telomer B alcohol/kg dw treated sterile soil; 10 pg 8-2 telomer B acid /kg dw; 10 pg 8-2 unsaturated telomer B acid/kg dw; and 10 pg perfluorooctanoic acid/kg dw, heptadecafluorononaoic acid, nonadecafluorodecanoic acid, and perfluoroundecanoic acid at 10 pg/kg dw all added to sterile soil. The test systems reported here were incubated in sealed containers at approximately 20C for at approximately 24 months. Two incubation chambers from each of the groups were removed at appropriate time intervals and the soil samples were extracted and analyzed for potential metabolites. In addition to the day zero samples, eight samplings were performed with all of the groups. Three additional sampling intervals were performed with only the untreated control (1) and 200 mg test substance/kg dw treated live soil (2) groups. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 18- Additional untreated and test substance treated soil chambers were prepared for use as matrix fortification samples, biomass determinations, viability controls and to monitor aerobic conditions as necessary. * The test groups were prepared using 25 grams (dry weight equivalent) of soil. While this amount of soil is less than die 50 to 200 grams specified in the OECD 307 guideline, it was chosen to allow for 1) the extraction of the entire test chamber contents and internal surfaces; 2) sufficient headspace to minimize the need for active aeration which could result in a loss of potential transformation products. METHODS AND MATERIALS The test system and study conditions are based on the OECD Guideline for Testing of Chemicals, Guideline 307, Aerobic and Anaerobic Transformation in Soil (1). As this guideline is designed for low molecular weight substances, but not for polymers, adaptations of the method may be necessary for feasibility reasons. Nevertheless, the study was carried out in such a way that the recommendations given in the guideline were followed as closely as possible. Test Substance The following substance was used in this study: ID:( ) Date received: 2 November 2004 Wildlife International Ltd. ID: 6918 1 Measured Analytes Name: 8-2 Telomer B Acid, 8-2 Fluorotelomer Acid Synonym used in Report: 8-2 FTA CAS Name: Decanoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro-(8CI,9CI) CAS Number: 27854-31-5 Molecular Weight: 478.1 g/mole ID: H-26210 Date received: 1 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 97%; 3% 2H-Hexadecafluoro-2-Decanoic Acid Company Sanitized. Does not contain TSCA CBI. W ildlife International, L td. Project No.: 112E-108 - 19- Description: Solid Wildlife International Ltd. ID: 6826 Name: 8-2 Telomer B Alcohol, 8-2 Fluorotelomer Alcohol Synonym used in Report: 8-2 FTOH CAS Name: 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoro- (7CI, 8CI, 9CI) CAS Number: 678-39-7 Molecular Weight: 464.11 g/mole ID: H-24691 Date received: 1 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99.2% Description: Solid Wildlife International Ltd. ID: 6828 Name: 8-2 Unsaturated Telomer B Acid, 8-2 Fluorotelomer Unsaturated Acid Synonym used in Report: 8-2 FTUA CAS Name: 2-Decenoic acid, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-hexadecafluoro- (9CI) CAS Number: 70887-84-2 Molecular Weight: 458.1 g/mole ID: H-26211 Date received: 1 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 98%; 0.5% 8-2 FTOH; 1.5% unknown Description: Solid Wildlife International Ltd. ID: 6829 Name: N-Perfluorooctanoic Acid Synonym used in Report: PFOA CAS Name: Octanoic acid, pentadecafluoro- (8CI, 9CI) CAS Number: 335-67-1 Molecular Weight: 414.07 g/mole ID: H-24749 Date received: 10 September 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99.2% Description: Solid Wildlife International Ltd. ID: 6849 Name: Heptadecafluorononaoic Acid, Perfluorononanoic Acid Synonym used in Report: PFNA CAS Name: Nonanoic acid, heptadecafluoro-(8CI, 9CI) CAS Number: 375-95-1 Molecular Weight: 464.08 g/mole ID: H7568 Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -20- Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6944 Name: Nonadecafluorodecanoic Acid, Perfluorodecanoic Acid Synonym used in Report: PFDA CAS Name: Decanoic acid, nonadecafluoro (6CI, 7CI, 8CI, 9CI) CAS Number: 335-76-2 Molecular Weight: 514.08 g/mole ID: R11K Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6943 Name: Perfluoroundecanoic Acid, Perfluoroundecanoic Acid Synonym used in Report: PFUA CAS Name: Undecanoic acid, heneicosafluoro-,ammonium salt (9CI) CAS Number: 4234-23-5 Molecular Weight: 564.09 g/mole ID: Q30G Date received: 30 November 2004 Expiration/reanalysis date: 12 October 2007 Purity: 99+% Description: Solid Wildlife International Ltd. ID: 6945 Name: 2H, 2H, 3H, 3H Pentadecafluoro-Decanoic acid Synonym used in Report: 7-3 Acid CAS Name: Decanoic acid, 4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-pentadecafluoro- CAS Number: 812-70-4 . Molecular Weight: 442.12 g/mole ID :26833 Date received: 20 April 2006 Expiration/reanalysis date: Not provided Purity: Not provided Description: Solid Wildlife International Ltd. ID: 7592 Name: 1-Perfluoro heptyl ethanol, 7-2 Secondary Fluorotelomer Alcohol Synonym used in Report: 7-2 sFTOH CAS Name: Not provided CAS Number: Not provided Molecular Weight: 414.11 g/mole ID :27497 Lot: 72SFTOHGA05 Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. -21 - Date received: 20 April 2006 Expiration/reanalysis date: Not provided Purity: Not provided Description: Liquid Wildlife International Ltd. ID: 7593 Internal Standards Name: N-Perfluorooctanoic Acid [l,2-di-13C] Synonym used in Report: 2-13C PFOA CAS Name: NA CAS Number: NA ID: H-25617 Dates received: 1 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 and 31 January 2007 Purity: 96.4% (not given for WIL 7591) Description: Solid Wildlife International Ltd. ID: 6827,7591 Standard Reanalysis Date: August 2007 Purity: Confirmed Expiration Date: August 2010 Name: ID, ID, 2D, 2D, 3-13C-Heptadecafluorodecanol Synonym used in Report: HDFD CAS Name: NA CAS Number: NA ID: H-25433 Dates received: 10 September 2004 and 20 April 2006 Expiration/reanalysis date: 12 October 2007 Purity: 100% (not given for WIL 7590) Description: Solid Wildlife International Ltd. IDs: 6850,7590 Project No.: 112E-108 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -22- Test Soils Four freshly collected United States (U.S.) soils were used to evaluate the test substance under aerobic and anaerobic conditions. The soils were collected from each of the following soil orders: Soil O rder Percent of US Land* Alfisol 13 Mollisol 25 Inceptisol 16 Ultisol 13 Percent o f total land in the US, from Foth, H.D. 1990. Fundamentals o f Soil Science 8 ed. John Wiley & Sons, New York) The soils were processed between 6 and 22 days after collection. Processing included removing vegetation, larger soil fauna, stones and passing the soil through a 2 mm sieve. The soil used in the sterile groups was sterilized by cobalt irradiation. The soils were stored in the dark at 4 2 C. Characterization of the soils was conducted by Agvise Laboratories (Northwood, North Dakota, USA). The following is a list of the minimum physicochemical properties of the soil. Texture (i.e., percentage of sand, silt and clay) (NUT.02.06) pH (NUT.02.05) Organic carbon (NUT.20.20) Bulk density (NUT.02.10) Field moisture capacity (% moisture at 1/3 bar) Cation exchange capacity (NUT.02.03) The percent moisture and water holding capacity (ISO 14240-2; Annex A) of each of the soil types was determined. In addition, the microbial biomass (ISO 14240-2; fumigation-extraction) of a live sample of each soil type was determined. The soil microbial biomass was determined at the beginning of the test prior to adding the test substance and in test and control soil samples at 4, 6 and 12 months using the fumigation-extraction method. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -23 - Test Apparatus and Conditions The test chambers were 250 mL glass serum bottles (Wheaton, Milleville NJ). The chambers were sealed with butyl rubber septa (Wheaton, Milleville NJ) that were covered with aluminum foil and aluminum crimp caps (Wheaton, Milleville NJ). The test chambers were identified by project number, test substance ID, test concentration, and unique identifier and incubated statically at approximately 20 2C. Soil Pre-Incubation The soil moisture of each test soil content was adjusted to 40 to 60% of water holding capacity (which is equivalent to a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The test soil then was allowed to warm to the test temperature for at least 2 days. A pre-incubation period of at least 7 days for the aerobic soils and at least 14 days for the anaerobic soils was performed under the appropriate conditions (i.e. temperature, soil moisture content, aerobic or anaerobic). Approximately 25 grams dry weight equivalent of test soil was added to the test chambers. Sterile soil treatments were dosed at approximately 200 mg/kg (dw) with both chloramphenicol (Aldrich, 98%, CAS 56-75 7) and cycloheximide (Sigma, min. 94%, CAS 66-81-9) to inhibit microbial growth. The chambers to be incubated under aerobic conditions were sealed with aluminum foil-lined septa and incubated. The chambers to be incubated under anaerobic conditions were flushed with anaerobic mixed gas, sealed with aluminum foil-lined septa and incubated. The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC). The soil moisture content of each chamber was checked and adjusted, if necessary, once during the pre-incubation period by weighing the test vessels. Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water losses. Preparation of the Test Chambers The soil moisture content was checked and adjusted, if necessary, prior to the addition of the test substance. Sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) was added as necessary to compensate for water. Anaerobic treatments and controls were prepared within an anaerobic environment and stored in a temperature controlled room. The test substance was applied to the soil within the test chambers as described below. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -24- Background Blank Control: Soils with no test substance added was tested in duplicate to check for background concentrations of analytes (see Sampling and Measurements). The entire bottle was extracted after a sample of the headspace had been taken. Test S ubstance: The test substance dosed treatments were prepared by dosing approximately 25 grams (dry weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The entire bottle was extracted after a sample of the headspace had been taken. Abiotic Controls: The abiotic controls were prepared by dosing approximately 25 grams (dry weight equivalent) of 6Co-sterilized soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The entire bottle was extracted after a sample of the headspace had been taken. Spike Recovery Controls: The spike recovery controls were prepared by dosing approximately 25 grams (dry weight equivalent) of Co-sterilized soil with appropriate volumes of 8-2 FTOH stock (250 pg/mL in ethanol) and fluorinated acid stocks (10 pg/mL in water). The stocks were injected directly into the soil using a glass microsyringe. The test chambers were immediately sealed with septum lined with aluminum foil and the contents of the chambers were mixed. The entire bottle was extracted after a sample of the headspace had been taken. Viability Controls (untreated): The metabolic activity of untreated test soil was assessed on a monthly basis. Duplicate incubation chambers for each test soil and condition (aerobic and anaerobic) were dosed at approximately 100 mg/kg dw with a combination of radiolabeled and non-labelled glucose. The evolved l4C 02 (aerobic and anaerobic conditions) and 14CH4 (anaerobic conditions) was measured and the percent biodegradation calculated. Aerobic Controls ('treated'): The aerobic controls were prepared by dosing approximately 25 grams (diy weight equivalent) of soil with sufficient test substance to deliver 200 mg/kg dry weight. The test substance was administered by direct weight addition. The test chambers were sealed with septum lined with aluminum foil, mixed and incubated. The oxygen content of a sacrificial chamber was measured at monthly intervals. Maintenance of Test Chambers The soil moisture content was maintained in the optimal microbial growth range of 40 to 60% water holding capacity (WHC; a pF of between 2.0 and 2.5, or from 0.1 to 0.33 bar). The soil moisture content was initially checked and adjusted, if necessary, at 1 to 2 week intervals by weighing the test vessels but was changed to monthly intervals based on the observed weight losses. Adding sterile-filtered demineralized water (degassed sterile-filtered demineralized water for the anaerobic soils) compensated for water losses. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -25- The oxygen content in the headspace of two aerobic control chambers was assessed on a monthly basis to ensure aerobic conditions. If the mean measured oxygen content within the control chambers was less than 19%, aerobic test chambers was aerated with filter-sterilized, oil-free air. Test chambers did not have to be opened to add water or for monitoring purposes. Soil moisture content was adjusted by injecting the appropriate amount of sterile water through the septa and onto soil. The headspace oxygen content was measured by inserting the probe of an oxygen analyzer through the septa and into the headspace of the aerobic control chamber. Sampling and Measurements Duplicate chambers from each of the control, treated and spike recovery soils were extracted and analyzed. Sampling intervals were at 0, 1 and 2 weeks and 1, 2,4, 6, 9 12, 15, 18 and 24 months for aerobic. Potential volatile transformation products in the headspace of the soils chambers were collected using a C18 cartridge. At each sampling time prior to opening the test vessel, the septum was pierced using a needle connected to a C18 cartridge and syringe. A 20-mL volume of headspace gas from within the chamber was pulled through the Cl 8 cartridge using the attached syringe. The Cl 8 was subsequently extracted with ACN and analyzed. Sample extracts were stored at -10C or lower if analysis is delayed more than 24 hours. Extracts were analyzed for: 1. 8-2FTOH 2. 8-2 FTA 3. 8-2FTUA 4. PFOA 5. PFNA 6. PFDA 7. PFUA 8. 7-2 sFTOH 9. 7-3 Acid Quality Criteria Recovery for a given sampling time point should range between 70 to 120% for the analysis of the 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFNA, PFDA, PFUA, and PFOA from the spike recovery control samples. These ranges should be interpreted as targets and should not be used as criteria for acceptance of the test. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -26- Viability Assessments On a monthly basis, duplicate incubation chambers for each test soil and condition were dosed with 1 rnL of the stock solution containing both radiolabelled and non-labelled forms of d-glucose which resulted in a concentration of approximately 3000 mg/kg dw. The headspace of each of the aerobic test chambers was continuously purged at an air-flow rate of approximately 1 bubble per second. The air exiting the test chambers was be passed through a series of gas washing bottles with at least one containing approximately 100 mL of 1.5 N KOH. The headspace of each of the anaerobic chambers was continuously purged with a flow of nitrogen and passed through a gas collection system consisting of two sets of C 02 traps and a combustion apparatus. The displaced gases initially passed through one empty bottle followed by two bottles each containing approximately 100 mL of 1.5N KOH (C02 trapping solution) followed by another empty bottle. The gas was combined with a flow of oxygen and channeled through a quartz column that was packed with cupric oxide and maintained at approximately 800C in a tube furnace to combust methane to C 02. The gas exiting the combustion column was passed through an empty bottle followed by two additional C 02 traps. The C 02 traps connected to the aerobic chambers and the l sl C 02 trap in each set (before and after furnace) of the anaerobic mineralization apparatus were removed for analysis and replaced at least weekly. Three replicate 1 mL aliquots of C 0 2 trapping solution were analyzed for radioactivity by liquid scintillation counting (LSC; Packard Tri-Carb Liquid Scintillation Counter). The assessment was terminated when 50% of the initial activity was recovered as mineralization products or after 28 days. RESULTS Test Conditions Room temperatures during the acclimation period for all four soils ranged from 18 to 22 C. Temperatures recorded during the test for all four soils ranged from 17 to 25 C. Incubation temperatures outside of the range 18 to 22 C were observed three times over the duration of the study. The frequency of measurements performed on the test soils is presented in Table 1. The moisture contents of the soils were adjusted as needed based on soil moisture content determinations. Each vessel required water additions at least once during the initial 12-month test period, but no additions were required for shorter incubation times. The maximum amounts of water added to any vessel over 12 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. projectNo.: 112E-108 -27- and 24 month periods were 1.2 and 3.9 mL, respectively. The oxygen content in the headspace of the two aerobic control chambers used to assess aerobic conditions is presented in Table 2. Characterization of Soils The test soils were characterized before the start of acclimation, and the results are presented in Table 3. The results of the viability assessments are presented in Tables 4-7. The results of microbial biomass determinations performed at the start of the test, during the test, and at the end of the test are presented in Table 8. Sample Processing and Analysis Soil chambers were either processed immediately upon receipt or removed from frozen storage and prepared for analysis of the candidate analytes 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. Headspace Analyses Prior to opening the soil chambers, a 20-mL volume of headspace gas was sampled from each chamber for 8-2 FTOH using a gas-tight syringe (Becton Dickinson, 10 mL with Luer-LokTM tip), hypodermic needle (Becton Dickinson, 18G 114) and a C]8 cartridge (600 mg, Alltech Maxi-Clean). Each sampled C|g cartridge was extracted with 10 mL of acetonitrile (Burdick & Jackson, HPLC-Grade, CAS No. 75-05-8). The extracts were fortified with 100 pL of a 0.100 mg HDFD/mL internal standard in acetonitrile to yield an internal standard concentration of 100 ng/mL in each extract. The fortifications were performed using a gas-tight syringe (Hamilton 81000 Series). An aliquot of each sample extract was transferred into a glass autosampler vial, sealed and analyzed for 8-2 FTOH by GC/MS. A method outline and typical instrumental conditions for analysis of 8-2 FTOH in the headspace of the soil chambers are provided in Figure 1 and Table 9, respectively. Soil Analyses Following headspace sampling, the crimp seal from each soil chamber was removed and the aluminum foil liner was placed inside the test vessel. A 125-milliliter aliquot of acetonitrile extraction solvent (measured with a graduated cylinder) followed by a 10-milliliter aliquot of 200 mM sodium hydroxide (measured with a serological pipette and prepared with Aldrich, Lot No. 14227TO NaOH or Fisher, Lot No. 926471 and Burdick & Jackson HPLC-Grade reagent water, CAS Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -28- No. 7732-18-5) were added to each test vessel containing the soil sample. The acetonitrile extraction solvent contained 100 ng/mL of HDFD internal standard and was prepared by fortifying four liters of acetonitrile measured with a graduated cylinder with 400 pL of a 1.00 mg HDFD/mL primary stock' in isopropyl alcohol (Burdick & Jackson, HPLC-Grade, CAS No. 67-63-0) and a gas-tight syringe. The vessels were re-sealed and placed onto a gyratory shaker table (New Brunswick Scientific, Model G10) set for 250 rpm for three hours at room temperature. The vessels were removed from the gyratory table and allowed to settle for several minutes at ambient temperature. Using a volumetric pipette, a 10-mL aliquot of the extract from each vessel was transferred into a separate polypropylene centrifuge tube (Nalgene, 50-mL polypropylene Oak Ridge type, Catalog No. 3119-0050) containing 25 milligrams of ENVI-Carb (Supelco, 120/400). The test vessel, including the remainder of the extract, was replaced into frozen storage (operating range -10 to -20C). The extract aliquots were centrifuged (Sorvall RC5BPlus fitted with Sorvall SS-34 rotor) for 10 minutes at 12000 rpm (17000 g) and 20 degrees C. An aliquot of supernatant was diluted with reagent water containing 1.00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water and the diluted extract was analyzed for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by HPLC tandem mass spectrometry (LC/MS/MS). The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 pL of a 2-13C PFOA secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and either a gas-tight syringe or calibrated micropipettes. A second aliquot of supernatant was diluted with reagent water containing 1.00 ng/mL of HDFD internal standard and in the proportions 1 part extract: 1 part dilution water and the diluted extract was analyzed for 8-2 FTOH by LC/MS/MS. The dilutions were performed directly in HPLC autosampler vials using calibrated micropipettes. The dilution water was freshly prepared by fortifying 100 mL of reagent water with 100 pL of a HDFD secondary stock in acetonitrile using a borosilicate glass, Class A volumetric flask and gas-tight syringe. The remainder of the soil extract supernatant was decanted into glass scintillation vials (Fisher, 20-mL disposable, Catalog No. 03-337 7) and placed in frozen storage. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -29- At the request of the Sponsor, two additional analytes, 7-2 sFTOH and 7-3 Acid, were screened for in selected stored soil extracts collected prior to the Month 18 sampling interval using LC/MS/MS. The earlier sampling intervals included Day 0 and Months 2, 6, 9, 12 and 15. 7-2 sFTOH and 7-3 Acid were also screened for in soil extracts generated concurrent with the Months 18 and 24 sampling intervals. The soil treatments selected for the screen included one untreated control soil replicate and the live treated soil replicates in each of the four soil types. Three different sample preparation regimes were used. For concurrent analyses of 7-2 sFTOH and 7-3 Acid, an aliquot of soil extract was diluted with reagent water containing 100 ng/mL of HDFD internal standard and 1.00 ng/mL of 2-13C PFOA internal standard in the proportions 1 part extract: 3 parts dilution water. This preparation was used for Month 18 and batch analyses on the earlier sampling intervals, except Day 0. For 7-2 sFTOH analyses only, an aliquot of soil extract was diluted with reagent water containing 1.00 ng/mL of HDFD internal standard and in the proportion of 1 part extract: 3 part dilution water. For 7-3 Acid analyses only, an aliquot of soil extract was diluted with reagent water containing 1.00 ng/mL of 2-13C PFOA internal standard and in the proportions 1 part extract: 3 parts dilution water. The latter two preparations were used for processing Day 0 and Month 24 samples where re-analyses due to instrumental or sample stability issues were required (e.g. 7-2 sFTOH stability in the dilutions was suspect). Each of the dilution solvents in reagent water was freshly prepared before use. A method outline for the analysis 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts by LC/MS/MS is presented in Figure 2. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA are provided in Table 10. Typical instrumental conditions for LC/MS/MS analysis of 8-2 FTOH are provided in Table 11. Typical instrumental conditions for LC/MS/MS analysis of 7-2 sFTOH and 7-3 Acid are provided in Table 12. Stock and Calibration Standard Preparation Individual component primary stock solutions (1.00 mg/mL) were prepared for each analyte and internal standard from the neat materials using an analytical balance and volumetric glassware. Individual component secondary stock solutions (100, 10.0, 1.00 and 0.100 pg/mL) were also prepared by serial dilution. The 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 2-13C PFOA and HDFD (for 24 month interval only) primary stock solutions were prepared in acetonitrile. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -30- The 8-2 FTOH, 7-2 sFTOH and HDFD (except as noted for the 24 month interval) primary stock solutions were prepared in isopropyl alcohol. All secondary stocks were serially diluted with acetonitrile. The 100-pg/mL HDFD secondary stock solution was used to fortify the internal standard into soil extraction solvent, calibration standards containing 8-2 FTOH and 7-2 sFTOH and dilution solvents for analyses of soil extracts for these analytes. For GC/MS analyses of 8-2 FTOH in the headspace gas of soil test chambers, 8-2 FTOH calibration standards in acetonitrile (25.0, 50.0, 100, 250 and 500 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks. Representative ion chromatograms for 8-2 FTOH calibration standards by GC/MS are presented in Figures 3 and 4. For LC/MS/MS analyses of 8-2 FTOH in soil extracts, 8-2 FTOH calibration standards in 50: 50 (v/v) acetonitrile: reagent water (10.0, 25.0, 50.0, 100, 250 500 and 1000 ng 8-2 FTOH/mL) and each fortified at 100 pg HDFD/mL internal standard, were prepared from the appropriate individual component stocks. Representative ion chromatograms for 8-2 FTOH calibration standards by LC/MS/MS are presented in Figures 5 and 6. For 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses of soil extracts, a 100pg/mL -combined stock of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks. Secondary combined acid stocks in acetonitrile (10.0, 1.00 and 0.100 pg/mL) were prepared by serial dilution from the 100 pg/mL combined stock solution. Combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards in 25: 75 (v/v) acetonitrile: reagent water (0.100, 0.250, 0.500, 0.750 and 1.00 ng/mL) and each fortified with 0.75 ng 2-13C PFOA/mL internal standard were prepared from the 0.100-pg/mL combined stock. A 5.00 ng/mL combined 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standard in 25: 75 (v/v) acetonitrile: reagent water was prepared from the 1.00pg/mL combined stock. All stock and calibration standards were stored under refrigerated (-4 degrees C) conditions when not in use. For 7-3 Acid and 7-2 sFTOH analyses of Months 2, 6, 9, 12, 15, 18 and 24 (7-3 Acid only) soil extracts, two sets of combined stocks were prepared. A 10-pg/mL combined stock of 8-2 FTA, Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -31 - 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in acetonitrile was prepared from the individual component primary stocks. Secondary combined stocks in acetonitrile (1.00 and 0.100 pg/mL) were prepared by serial dilution from the 10.0-pg/mL combined stock. A 10.0-p.g/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile was prepared from the individual component primary stocks. Calibration standards in 25: 75 (v/v) acetonitrile: reagent water containing 0.75 ng 2-13C PFOA/mL and 100 ng HDFD/mL internal standards were prepared from the 0.100 and 1.00-pg/mL combined perfluorinated carboxylic acid (PFCA) and 10.0-pg/mL combined telomer alcohol stock solutions, respectively. The nominal concentrations of 7-3 Acid and 7-2 sFTOH in the calibration standard set (7-3 Acid/7-2 sFTOH) were 0.100/5.00, 0.250/10.0, 0.500/25.0, 0.750/35.0, 1.00/50.0 and 5.00/250 ng/mL. Due to stability concerns, fresh preparations of 7-2 sFTOH calibration standards were prepared from the 10.0-pg/mL combined stock of 8-2 FTOH and 7-2 sFTOH in acetonitrile for analyses of 7-2 sFTOH in Month 24 soil extracts. The nominal concentrations of 7-2 sFTOH were as specified above. For 7-3 Acid analyses of Day 0 soil extracts, a combined calibration standard for set for 7-3 Acid and 7-2 sFTOH only was prepared as described above from individual component 7-3 Acid (0.100 and 1.00-ng/mL) and 7-2 sFTOH (10.0-ng/mL) secondary stock solutions. Only 7-3 Acid results were reported from this standard set due to a delay in instrumental analyses. For 7-2 sFTOH analyses, another set of standards was freshly prepared from the 10.0-pg/mL 7-2 sFTOH secondary stock solution. Representative ion chromatograms for 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA calibration standards by LC/MS/MS are presented in Figures 7-22. Calibration Curves and Limits of Quantitation Calibration standard sets for a given analysis were injected at the beginning an<l end of each analytical sequence with one calibration standard injected after a maximum of every five study samples. For 8-2 FTOH analyses in the headspace o f test vessels by GC/MS, linear regression equations were generated using adjusted peak areas (for the internal standard response ratio) versus the respective concentration of the calibration standards. The concentration o f 8-2 FTOH in the sampled headspace of the soil test vessels was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. The concentrations were converted to a mass basis using the appropriate volumes, weights and unit conversion factors. A representative calibration curve for 8-2 FTOH analyses by GC/MS is presented in Figure 23. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -32- For soil extract analyses of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA,7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS, weighted linear regression equations were generated using peak area response ratios (analyteinternal standard) versus the respective concentration ratios (analyteinternal standard) of the calibration standards. The concentration of each analyte in the samples was determined by substituting the peak area response ratios obtained for the samples into the applicable linear regression equation. Representative calibration curves for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA analyses by LC/MS/MS are presented in Figures 24-32, respectively. The limits of quantitation (LOQ) for these analyses were defined on a low-level standard equivalent basis and were calculated for 8-2 FTOH in the sampled headspace and for each analyte in soil as the product of the low-level standard concentration and the dilution factor of the untreated control samples. The LOQ for 8-2 FTOH in a 20 mL headspace gas aliquot was 12.5 ng/mL sampled headspace gas, or 0.250 pg for a 20-mL sample. The LOQ was 2.0 pg/kg for soil analyses of 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA. The LOQs for soil analyses of 8-2 FTOH and 7-2 sFTOH were 100 and 100 pg/kg, respectively. Statistical derivation of the limits of detection (LOD) was completed for all analytes with the exception of 7-3 Acid and 7-2 sFTOH during method verification trials. The calculation for each analyte was based on three times the concentration equivalent of the standard deviation of five replicate analyses of a reagent blank. Method verification trials and derivation of statistical LODs for 7-3 Acid and 7-2 sFTOH was outside the scope of the study. Measured concentrations of the analytes are presented in mg/kg soil dry weight and pmol/kg soil dry weight in Appendix I and II, respectively. Measured concentrations of 7-2 sFTOH and 7-3 Acid in the untreated soils were less than the LOQ and are not presented. Example calculations are presented in Appendix III. Representative chromatograms are presented for each of the four treatment groups in aerobic alfisol soil. Similar chromatographic characteristics were evident for the aerobic ultisol, mollisol and inceptisol soils. Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of untreated control, fortified control, sterile and live samples treated with J from Day 0 of the test are presented in Figures 33, 53, 69 and 85, respectively. Representative ion chromatograms for Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -33 - 8-2 FTOH in the sampled headspace of untreated control and live samples treated with Month 24 of the test are presented in Figures 43 and 95, respectively. jfrom Representative ion chromatograms for 8-2 FTOH by GC/MS in the sampled headspace of fortified control and sterile samples treated withC from Month 12 of the test are presented in Figures 61 and 77, respectively. Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from untreated control samples from Day 0 and Month 24 of the test are presented in Figures 34-42 and 44-52, respectively. Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from fortified control samples from Day 0 and Month 12 of the test are presented in Figures 54-60 and 62-68, respectively. Representative ion chromatograms for 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from sterile samples treated with L jfrom Day 0 and Month 12 of the test are presented in Figures 70-76 and 78-84, respectively. Representative ion chromatograms for 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts from live samples treated with(. Jfrom Day 0 and Month 24 of the test are presented in Figures 86-94 and 96-104, respectively. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -34REFERENCE Project No.: 112E-108 1 Organisation for Economic Cooperation and Development. April 2002. Aerobic and Anaerobic Transfortnation in Soil. OECD Guideline 307. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -35 - Table 1 Frequency of Measurements1 Interval Activity Biomass Soil moisture Headspace Soil Analytical oxygen metabolic interval Day 0 Week 1 Week 2 Week 4 Week 6 V V V V V content V activity V V V V V Month 2 Month 3 VV VV VV V Month 4 V V V V V Month 5 VV V Month 6 V V V V V Month 7 VV V Month 8 VV V Month 9 Month 10 VV VV VV V Month 11 V' V 3-- Month 12 V V V V V Month 15 VV Month 18 V VV Month 21 V Month 23 V Month 24 V VV Additional chambers for the measurement of biomass and headspace oxygen content beyond the initial 12 month test period were not prepared at the start of the study, Company Sanitized. Does not contain TSCA CBI. W ildlife InternationalLtd. Project No.: 112E-108 Interval Month 1 Month 2 Month 3 Month 4 Month 5 Month 6 Month 7 Month 8 Month 9 Month 10 Month 11 Month 12 -36- Table 2 Test Chamber Headspace Oxygen Content Alfisol 20.8 21.0 20.9 20.8 20.8 20.9 20.8 20.8 20.8 20.9 20.8 20.9 Average Oxygen Content (%) Inceptisol Mollisol 20.8 20.7 20.9 20.7 20.9 20.6 20.8 20.8 20.7 20.7 20.9 20.7 20.7 20.8 20.8 20.9 20.8 20.8 20.9 20.8 20.8 20.8 20.9 20.8 Ultisol 20.8 20.7 20.6 20.8 20.8 20.8 20.7 20.8 20.8 20.9 20.8 120.8 ' Company Sanitized. Does not contain TSCA CBI. W ildlife InternationalLtd. Project No.: 112E-108 -37Table 3 Soil label Texture class Alfisol Inceptsol Ultisol Mollisol Sandy loam Loam Sandy loam Sandy clay loam 1- cation exchange capacity 2- pH in 1:1 soil water ratio Selected Soil Properties Organic carbon content % 1.4 2.0 2.9 2.1 Sand Silt Clay % 65 24 11 45 32 23 55 31 14 59 18 23 CEC1 meq/100 g 7.8 16.9 7.9 17.7 Water holding capacity pH2 % 5.8 54.5 6.6 43.3 4.8 59.3 6.9 63.2 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -38- Table 4 Soil Viability by Glucose Mineralization: Alfisol Month Glucose Mineralization Rate Aerobic Anaerobic 1 1.83 1.81 2 1.79 2.68 3 2.45 7.34 4 2.73 7.36 5 2.54 4.17 6 2.60 3.00 7 3.99 NA 8 3.79 NA 9 7.57 NA 10 2.64 NA 11 2.39 NA 12 2.00 NA 15 1.64 NA 18 1.81 NA 24 1.81 NA - (percent biodegradation/elapsed time in days) x 100 Table 5 Soil Viability by Glucose Mineralization: Inceptisol Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd Project No.: 112E-108 -39 - Table 6 Soil Viability by Glucose Mineralization: Ultisol Month Glucose Mineralization Rate1 Aerobic Anaerobic 1 2.23 2.22 2 2.56 2.39 3 1.67 2.86 4 1.95 4.22 5 4.04 5.41 6 2.46 3.80 7 3.79 NA 8 7.33 NA 9 7.37 NA 10 8.83 NA 11 2.99 NA 12 3.61 NA 15 8.19 NA 18 2.37 NA 24 3.65 NA (percent biodegradation/elapsec time in days) x 100 Table 7 Soil Viability by Glucose Mineralization: Mollisol Month Glucose Mineralization Rate1 Aerobic Anaerobic 1 2.36 1.85 2 2.54 2.99 3 1.72 2.57 4 3.63 3.99 5 4.06 4.82 6 3.66 4.16 7 3.74 NA 8 3.95 9 3.60 10 7.51 11 2.73 12 1.78 15 3.59 18 2.11 1 24 (,p_e_r_c_e_n.t,biod, e--grad7--at:--ion/el-a--p-s-e--d time in days) 1.81 x 100 NA NA NA NA NA NA NA NA p. 40 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -40- Table 8 Soil Microbial Biomass (|xg/g) (ISO 14240-2; fumigation-extraction) ALFISOL Aerobic___________ Anaerobic_____ Treated Untreated Treated Untreated Post Inc. - 244 - 128 Month 4 317 323 435 287 Month 6 680 279 593 645 Month 12 769 675 NA NA NA - Not analyzed INCEPT1SOL ______ Aerobic_________ Anaerobic Treated Untreated Treated Untreated - 131 - 132 138 109 184 106 432 483 423 467 504 564 NA NA I p. 41 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -41 - Table 8 (continued) Soil Microbial Biomass (pg/g) (ISO 14240-2; fumigation-extraction) MOLLISOL Aerobic___________ Anaerobic_____ Treated Untreated Treated Untreated Post, Inc. - 190 - 53 Month 4 216 218 218 205 Month 6 243 259 267 270 Month 12 714 734 NA NA NA - Not analyzed ULTISOL ______ Aerobic Anaerobic Treated Untreated Treated Untreated - 312 - 54 36 31 26 34 486 194 500 407 565 570 NA NA p. 42 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. Project No.: 112E-108 -42Table 9 Typical GC/MS Operational Parameters for Analysis of 8-2 FTOH in Headspace Gas Instrument: Detector: Analytical Column: Injector Temperature: Oven Temperature Profile: Hewlett-Packard Model 5890 Series II Gas Chromatograph (GC) Hewlett-Packard Model 5971 Mass Selective Detector Phenomenex Zebron ZB-5 (30 m x 0.25 mm, 0.25-pm film thickness). 150C Initial temperature: 50C Initial hold time: 2.00 minute Ramp: Rate fC/minutei 20.00 50.00 Final Temperature l'Ci 100 300 Detector Temperature: Carrier Gas: Septum Purge: Split Vent: Injection Volume: Data Acquisition Mode: Final hold time: 3.00 minute 280C Helium, ~1 mL/min; CHP = ~14 psi Helium, ~5 mL/min ~40 mL/min 2 pL (2 stops w/ 10 pL syringe), splitless SIM Acquisition Ions: Peak Retention Times: Component 8-2 FTOH HDFD Quantitation 31 m/z 33 m/z Confirmation 95 m/z 98 m/z 8-2 FTOH: Approximately 5.0 minutes HDFD: Approximately 5.0 minutes p. 43 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -43- Table 10 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in Soil Extracts Instrument: Analytical Column: Column Temperature: Mobile Phase: Gradient Elution Profile: Injection Volume: Monitored Ion Transitions: PFOA 2-13 PFOA (internal standard) 8-2 FTA 8-2 FTUA PFNA PFDA PFUA LC/MS/MS Analog Parameters: Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode. Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size) 40C A: 0.1% Formic Acid in Reagent Water B: Acetonitrile Time /min') 0.00 1.00 8.00 9.00 9.10 14.00 15.00 20.00 50.0 pL Flow Rate (uL/minl 250 250 250 250 250 250 250 250 Percent A Percent B 70.0 30.0 70.0 30.0 15.0 85.0 15.0 85.0 5.0 95.0 5.0 95.0 70.0 30.0 70.0 30.0 Ql 413.0 amu 415.0 amu 477.0 amu 457.0 amu 463.0 amu 513.0 amu 563.0 amu Q3 369.0 amu 370.0 amu 393.0 amu 393.0 amu 419.0 amu 469.0 amu 519.0 amu Dwell Time 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec 250 msec Retention Time 9.82 min 9.82 min 12.2 min 12.3 min 10.6 min 11.2 min 11.8 min IS = -4500 TEM = 400 DP = -10 FP = -140 EP = -10 CE = -15 CXP = -25 CUR = 8 CAD = 4 NEB = 12 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Projectn0.: 112E-108 -44Table 11 Typical LC/MS/MS Operational Parameters for Analysis of 8-2 FTOH in Soil Extracts Instrument: Analytical Column: Guard Column: Column Temperature: Mobile Phase: Isocratic Profile: Injection Volume: Monitored Ion Transitions: 8-2 FTOH HDFD (internal standard) LC/MS/MS Analog Parameters: Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode. THERMO EC Betasil C-18 (50 mm x 2.1 mm, 5-pm particle size) Javelin Betasil C-18 (20 mm x 2.1 mm) 40C A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile Time (min) Flow Rate CuL/min) Percent A Percent B 0.00 250 6.00 250 30.0 70.0 30.0 70.0 25.0 pL Q1 523.1 amu 528.1 amu IS = -4500 TEM = 450 DP = -25 FP = -250 EP = -10 Q3 58.9 amu 58.9 amu Dwell Time 500 msec 500 msec . Retention Time 4.18 min 4.15 min CE = -50 CXP = -10 NEB =12 CUR = 7 CAD = 6 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -45Table 12 Typical LC/MS/MS Operational Parameters for Analysis of 7-3 Acid and 7-2 sFTOH in Soil Extracts Instrument: Analytical Column: Column Temperature: Mobile Phase: ' Gradient Elution Profile: Injection Volume: Reported Ion Transitions: 7-3 Acid 2-13 PFOA (internal standard) 7-2 sFTOH HDFD (internal standard) LC/MS/MS Analog Parameters: Agilent Technologies 1100 Series High Performance Liquid Chromatograph with an Applied Biosystems/MDS SCIEX API 3000 LC Mass Spectrometer equipped with a Perkin-Elmer Turbo IonSpray source. Operated in the negative ion multiple reaction-monitoring mode. Zorbax RX-C8 (150 mm x 2.1 mm, 3-pm particle size) 40C A: 0.15% Acetic Acid in Reagent Water B: Acetonitrile/0.15% Acetic Acid Time iminl 0.00 1.00 1.01 7.00 12.00 13.00 20.00 Flow Rate fuL/min) 250 250 250 250 250 250 250 Percent A 95.0 95.0 70.0 15.0 15.0 95.0 95.0 Percent B 5.0 50.0 30.0 85.0 85.0 5.0 5.0 50.0 pL Ql 441.0 amu 415.0 amu 473.0 amu 528.0 amu Q3 337.0 amu 370.0 amu 59.0 amu 59.0 amu Dwell Time 50 msec 50 msec 50 msec 50 msec Retention Time 11.6 min 10.0 min 11.9 min 12.1 min IS =-4500 TEM = 400 EP = -10 CUR = 7 CAD = 6 NEB = 132 7-3 Acid/ 2-13 PFOA DP = -40 FP = -350 CE = -15 CXP == -11 7-2 sFTOH/ HDFD -25 -250 -50 -10 p. 46 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -46- Project No.: 112E-108 Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers For each sample, fit a C18cartridge between the end of an appropriate volume syringe and a hypodermic needle. I With the plunger fully depressed, insert the needle of the disposable syringe through the septum of the sample and pull a full syringe volume. Remove needle, expel headspace air and repeat. i Detach Ci8cartridge and place in appropriate holder. Attach the barrel of a 10 mL disposable syringe to Cis cartridge and use a volumetric pipette to transfer acetonitrile extraction solvent into the syringe barrel. Insert the syringe plunger into the barrel and inject the acetonitrile through the Ci8cartridge into a 20-cc glass scintillation vial. Fortify the extract to contain 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro Decanol internal standard. Pipette an aliquot of the extract directly into an autosampler vial. Cap vial and submit for GC/MS analysis of 8-2 FTOH. All sample vials not analyzed immediately are place in refrigerated storage. i Store the remaining trap extract in scintillation vials under frozen conditions. Figure 1. Method flowchart for the analysis of 8-2 FTOH in the headspace of soil test chambers. p. 47 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -47- Project No.: 112E-108 Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA in soil extracts For each sample vessel, remove crimp seal and place foil liner into the vessel. Using a graduated cylinder, measure and transfer 125 milliliters of extraction solvent into each sample vessel. The extraction solvent consists of 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro Decanol internal standard in acetonitrile. Add a 10-mL aliquot of 200 mM NaOH into each sample. 4 Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Extract the samples for approximately three hours using a gyratory shaker table set for 250 rpm. '; Allow suspended material in extracts to settle. Remove a 10-mL aliquot of the extract using a serological pipette and transfer into a separate 50-mL plastic centrifuge tube preloaded with approximately 25 mg of Envi-CarbTM. Centrifuge samples at a setting of 12000 rpm for 10 minutes. Transfer extract supernatant for each sample into a separate labeled scintillation vial. 4 For 8-2 FTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL ID, ID, 2D, 2D, 3-13C Heptadecafluoro Decanol (HDFD) internal standard to produce a final solution composition of 1:1 (v/v) extract: water. For 7-2 sFTOH analyses, pipette an aliquot of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 100 ng/mL HDFD to produce a final solution composition of 1:3 (v/v) extracttwater. Perform dilution directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis of telomer alcohols. All sample vials not analyzed immediately, place in refrigerated storage. 4 For PFCA analyses of each sample, dilute an appropriate volume of the extract supernatant (or diluted extract, if applicable) with reagent water fortified with 1.00 ng/mL N-Perfluorooctanoic Acid, [1,2 di13C] internal standard to produce a final solution composition of 1:3 extract: water. Perform dilution directly in an autosampler vial, cap and vortex. Submit for HPLC/MS/MS analysis of PFCAs. All sample vials not analyzed immediately, place in refrigerated storage. 4 Re-crimp each sample vessel with a new aluminum crimp cap and the original septum. Store sample vessels and extract scintillation vials under frozen conditions. Figure 2. Method flowchart for the analysis of 8-2 FTOH, 7-2 sFTOH, 8-2 FTA, 7-3 Acid, 8-2 FTUA, PFOA, PFNA, PFDA and PFUA by LC/MS/MS in soil extracts. p. 48 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd, Project No.: 112E-108 -48- Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: T :\DATA\ALFI025\0101005.D Operator: DW Date Acquired: 20 Apr 106 5:41 pm Inatrument: GCMS#1 Sample Name: T A - 30 Mise Info: Vial Number : 1 25.0 ug a . i ./L [Abundance Ion 31.00 (30.70 to 31.70): 0101005.D 200 150 Time-->4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 Figure 3. Representative ion chromatograms for 8-2 FTOH in a low-level (25.0 ng a.i./mL) calibration standard by GC/MS. p. 49 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -49- Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: T:\DATA\ALFI025\0501009.D Operator: DW Date Acquired: 20 Apr 106 7:03 pm Instrument: GCMStfl Sample Name: TA-34 Misc Info: 500 ug a.i./L Vial Number : 5 Figure 4. Representative ion chromatograms for 8-2 FTOH in a high-level (500 ng a.i./mL) calibration standard by GC/MS. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -50- Project No.: 112E-108 Figure5. Representative ion chromatogram for 8-2 FTOH in a low-level (10.0 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -51 - Project No.: 112E-108 Figure 6. Representative ion chromatogram for 8-2 FTOH in a high-level (1000 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -52- Project No.: 112E-108 Figure 7. Representative ion chromatograms for 7-2 sFTOH in a low-level (5.00 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd, -53 - Project No.: 112E-108 Figure 8. Representative ion chromatograms for 7-2 sFTOH in a high-level (250 ng a.i./mL) calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -54- Project No.: 112E-108 Figure9. Representative ion chromatogram for 8-2 FTA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -55 - Project No.: 112E-108 Figure 10. Representative ion chromatogram for 8-2 FTA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TS C A CBI. W ildlife International, Ltd. -56- Project No.: 112E-108 Figure 11. Representative ion chromatogram for 7-3 Acid in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife InternationalLtd. -57- Project No.: 112E-108 Figure 12. Representative ion chromatogram for 7-3 Acid in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -58- Project No.: 112E-108 Figure 13 Representative ion chromatogram for 8-2 FTUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, -59- Project No.: 112E-108 Figure 14. Representative ion chromatogram for 8-2 FTUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -60- Project No.: 112E-108 U* |5S 1MwU tax IX i-------- j--------1-- 1-- r Figure 15. Representative ion chromatogram for PFOA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -61 - Project No.: 112E-108 Figure 16. Representative ion chromatogram for PFOA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. -62- Project No.: 112E-108 Figure 17. Representative ion chromatogram for PFNA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -63- Project No.: 112E-108 Figure 18. Representative ion chromatogram for PFNA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -64- Project No.: 112E-108 Figure 19. Representative ion chromatogram for PFDA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -65 - Project No.: 112E-108 Figure 20. Representative ion chromatogram for PFDA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, -66-, Project No.; 112E-108 Figure 21. Representative ion chromatogram for PFUA in a low-level (0.100 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -67- Project No.: 112E-108 Figure 22. Representative ion chromatogram for PFUA in a high-level (5.00 ng a.i./mL) combined PFCA calibration standard by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -68- p. 68 Project No.: 112E-108 IS Adjusted Peak Area Counts Concentration (pg 8-2 FTOH/L) Figure 23. Representative calibration curve for 8-2 FTOH by GC/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, -69- Project No.: 112E-108 Figure 24. Representative calibration curve for 8-2 FTOH by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -70- Project No.: 112E-108 Figure 25. Representative calibration curve for 7-2 sFTOH by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -71 - Project No.: 112E-108 Analvte A r e a / IS A r e a 042006_M12_TELALAE.rdb (8-2 (S)): "Linear" Regression ("1 / x" weighting): y = 0.116 x + 0.00123 (r = 0.9992) 0.80* 0.75 ' P 0.70 0.85 0.60 0.55 0.50' 0.45 0.40 0.35 0.30 0.25 0.20 0.15 0.10 0.05 0.0 0 - 0.5 1.0 1J5 2 0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 Anatyta Cone. / IS Cone. Figure 26. Representative calibration curve for 8-2 FTA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBi. W ildlife International, Ltd. -72- Project No.: 112E-108 Figure 27. Representative calibration curve for 7-3 Acid by LC/MS/MS. Company Sanitized. Does not contain TSC A CBt. W ildlife International, Ltd. -73- Project No.: 112E-108 Analvte A rea / ISA rea 042006_M12_TELALAE.ndb (8-2 (U)): "Linear* Regression ("1 DC weighting): y = 1.07 x + -0.00685 (r = 0.9996) 7.4 7.0 % 6.5 6.0 5.55.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5- 0.0- O.'s' ' ' llo ' ' ' 1 5 ' ' ' 0 ' Z 5 ' ' 3 0 ' 'a s ' ' 4 0 4.6 5.0 ' 5 5 ' 6.0 ' ' 'S __________________________________Analyte Cone. / IS Cone. Figure 28. Representative calibration curve for 8-2 FTUA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -74- x" 042006_M12_TELALAE.rdb(PFOA C-8): "Linear" Regression ("1 / weighing): y = 1.12 x + 0.0538 (r = 0.9985) Analvte A r e a / IS A r e a Analyte Cone. / IS Cone. Figure 29. Representative calibration curve for PFOA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -75 - Project No.: 112E-108 Analvte A rea /IS A rea 042006_M12_TELALAE.rdb (C-9 Add): "Linear" Regression (*1 / >Cwelghthg): y = 1.34 x + 0.00636 (r = 0.9991) 9.0 8.5 8.0 7.5 .X 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1. 0 0.5- 0.0-j* 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 __________________________________ Analyte Cone. I IS Cone. 6.5 Figure 30. Representative calibration curve for PFNA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI W ildlife International, Ltd. -76- Project No.: 112E-108 Analvte A r e a / IS A r e a Figure 31. Representative calibration curve for PFDA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, -77- Project No.: 112E-108 An alvte A rea / IS A rea Figure 32. Representative calibration curve for PFUA by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -78- Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: C:\HPCHEM\1\DATA\ALFI005\0701011.D Operator: fj1 Date Acquired: 30 Apr 105 4:10 pm Instrument: GCMS#2 Sample Name: 1570-TEX Misc info: Vial Number : 7 Figure 33. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8cartridge extract) from a Day 0 untreated control soil by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -79- Project No.: 112E-108 Figure 34. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 8-2 FTOH was not present (no peak at 4.18 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -80 - Project No.: 112E-108 Figure 35. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 7-2 sFTOH was not present (no peak at 11.9 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -81 - Project No.: 112E-108 Aim 'kanvtf ftmpto `iftTfr fto7042t09_OQ.TilAlXr.wllr Piali Man: '0*9 ( I f Maarfeal *477 M O ) 0 amw* Canw ant I12.0.l00,tl0-<UTC)` Awnttoon " sanpla )ndas> 14 l i a p t i Typ*i C oncentration! .l./fcH ank 0 .0 0 uqa I* , C a lc u la t e d Cene: M/A Acq. Datai Acq. Tlwai 0 4 /2 1 /0 * 0 7 : I0 i 04 PH 0600.00 H o d lC U d ; NDoulnac*h lnThvraf sahcotlodi:: I A ta* tn raah elrti H ut. tn o a th i! ; NT V indoui t x p a c ta d RTt Sap. H irtth: i Sap. I ilf h ti j t i p . Paak m e la i .! t i p . A dJ. l i t i o : II t i p . V a l. P a l l a i ;j Usa P a l a t i v i RT: HIo 24.04 11 5 0 .J ) )0.0 12.2 0O..2O0t 5 .0 0 4.00 2.00 Ho epa epa aae Un 000O' sooooo 7600.00 700000 " i n t . Typ*: P a ta n tlo n T laai A raat H aighti S ta ti T im i End TIm i No Peak H/A N/A H/A N/A un c o u n ts epa lift 1400 00 oooao gttOOGO w oooo * 400000 I400000 : 3600 00 ; 3000.00 ! 2500.00 3000 Q0 1600.00 1000.00 80000 Printing Date: Friday* April 22,2005 * ' U4 ..lL-jatiB-- 1U0l3.42.1S.ni9M Figure 36. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 8-2 FTA was not present (no peak at 12.2 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -82- Project No.: 112E-108 Figure 37. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 untreated control soil by LC/MS/MS. 7-3 Acid was not present (no peak at 11.6 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, Project No.: 112E-108 I*S*J Hem* `iMnfrf* 0 0 T I w i f h - *1*70" F i r *042109_00_TeLALA w flF^ PMkNHM.*-9<Ur !*<*} *457.00 CminuCm m M tl3*-10.0B.1t^turC )' AnMliVaA-** Saapl* tndcxi 14 Saapl* Typ*t C cncanccatian: M nk 0 .0 9 u} i . i . / L tO(WS C a lc u la t e d C onct N/A Acq. S at* : 04/21/0$ Acq. T taes O il 1 0 :9 4 PM SO*4 H 4llldi Bunching r a c ta t i N an Thraahold: Ax Thc*hol<d; Nua. smooth! AT Windows tx p c t* d AT: Sap. W idths S*p. W eights ta p . P*k A ati: Exp. A d). A atla: Exp. V al. p a tio : U ia H a l a t l v a AT: N* 1 2 0 1 .0 1 1009.61 1 3 0 .0 1 2 .3 0 .2 0 0 .0 1 9 .0 9 4 .0 0 3 .0 0 No cpa ep* sac a in 900*4 90*4 00*4 7 60*4 7 00*4 Ine. Typi ActCA tion T la t: Ar*at H aight: Start Tiaa: CM T lM l Mo 9oak N/A n/ a N/A N/A N/A a in c o u n ts epa am am 6 60*4 6 00*4 9.60*4 ' 6.00*4 460*4 400*4 60*4 0.00*4 2.60*4 200a4 I 60*4j 1.00*4. 6000.00 I Printing Dale: Friday April 22,2005 -83 - 00 6J4 7 *0 . y 10.19 11.19 1267. 1 9 .4 2 ,129 6 7 0 9 10 11 12 1J 14 IS 10 _________________ Tim*.nan_______ __________________________ _ ___ 17 . Figure 38. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. . 8-2 FTUA was not present (no peak at 12.3 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. I Utmr*5iMyS~aFflankttO^tJiVFto:^TT6S_CirTET3UE P**k Nan*: *PFOA C4* Mat*). 4t 30 '36 0 0 mu' CnvnaMtR-10e.1.1KMUTCr ANwMait:** I su?l* index: H I saapl Typ: lank j C oncentration: 0.00 uq 1.00*9* , 1 . / L . C a lc u la t e d C nct KM " Aeq. U t t l .' A eq. T in a I 01/21/09 O il 1 0 :04 PH 060*4 M odified: Bunching f a c t o r : Hoi T h reitto lrii Ar*a T hraahold: Hu*. fcaootha: RT Window: B x p * ct* d RT: Sop. W idth: Sp. H eighti ta p . r a e l R atio: Exp. A dj. R atio: Exp. V el. R atio: V** R l a tl v RT: NO 1 199.77 P 4 B .14 1 3 0 .0 9 .1 2 0 .2 0 0 .0 1 9 .0 0 4 .0 0 3 .0 0 No epa cpa Bln 0.00*4 ; A60*4 ! 0.00*4 I 7.60*4 7.00*4 : In c. Typet R e te n tio n T in*: MAarleqah:r; Scare Tioa: End T in i K* Peak KM nM HM K/A KM un c o u n ts epa Bln Bln 060*4 -j 000*4 ! 060*4 ij 600*4' 4.90*4 , 4.00*4 360*4 t " 300*4 3.90*4 200*4 190*4 600000 000 -84- Project No.: 112E-108 Figure 39. Representative ion chromatogram for PFOA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFOA was not present (no peak at 9.82 min). Company Sanitized. Does not contain TSCA CBI W ildlife International, Ltd. Project No.: 112E-108 if'r N*^v^i4J0 . It I PMkNm:'C<OAetf' M**<**>;*403 0/410.0*mu` !! CM*n*f'112i . 100.100,114UrCr Anrattttea** I S M p l* In d a x i ;j!! s a p l* T ypt i0la.n0k0 uq 'i C a l c u l a t e d C o n c i H/A Ac*. D ata: 04/21/O S i Acs. T i**r 0 7 i 1 0 :0 4 OH IK odifiadt Bunching r a c to r i W eis T h resh o ld : Ara fh re sb a ld s K\ia. la M t lt i : . *T Window: ' B x p a c ttd h r : " Sap. W idth! Sap. H aight i Exp. Oaak R a tio : Exp. A dj. R atio: Exp. v a l. R atio: Uaa R a la tiv a RT: Wo 3 0 .0 10. 0.20 0.01 5 .0 0 4 .0 0 3 .0 0 HO A rea: K alghe: S t a r t T ioai End T is e t Ho Saak X/A N/A N/A N/A N/A t7.00*4^ 0*4 j o.oom j S.Oa4 1 00*4 4.40*4 4 00*4 300*4 300*4 2 60*4 2.00*4 IS0*4 -85 - Printing Date: Friday, April 22,2005 . m.. 710 8.00 1 0 ^ 1 0 0 0 ^ 1 J 4 .1 2 .H .1 2 J 0 I3 .O 3 4 0 0 7 " f 10 11 12 13 14 "is"" io 17 " 10 * _______ T kw .mln Figure 40. Representative ion chromatogram for PFNA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFNA was not present (no peak at 10.6 min). Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd, -86- TSmtufMvJkJM (UobftWftr 7S.'*Wi4"d P*kMnr"C-IOAe^ U*m|**>'SllOf481.0 trau- C ennw il' llK.IM .IQ a.U tH U TC r AnnoHon." S ta p l* ln d *: )4 * M ft Typ: C o n c e n tra tio n ! >Unk 0 .4 0 9 a . i . / L 1C U S C *l u l t d c on e : D/A Ae q. D *tt 04/ 1I / 0* Acq . T i| O T tiO tO m ** 4 H 0d m 4 : Punching r i e t e n iH o lt T h (h ld : j A t T h re s h o ld ; 11 ] i u b . s * e o t h i I N T w in d o w : ; e*p*ctd pti I *p. w id th : u p . tig h t! . u p . r**k n o tio i U p. M J. U li ! U p. V l . M tioi 0s l i l i U y i A T : No 1 1( 7.10 I K .S O 1 10.0 )).; 0.20 0.01 *.00 4 .(0 1.B0 Ke 06*4 A tta* 00* 4 TBCW 4 7. 00*4 ! J u t. 7yr*r M t t n t l e n Ti m i ' At*: (K alghti - S t a l l Tine: End Ti m i W k n/ a n/A H/A N /A H/A un count* epa n ln un 8-00*4 , 4. 00*4 J 156*4 ' 100*4 2SO)4j 200*4 j 1S0*I t 00*4 SOQOOO 000 4- ' Printing Dale: Friday, April 22,2005 Project No.: 112E-108 To"iV` to To Figure 41. Representative ion chromatogram for PFDA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFDA was not present (no peak at 11.2 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. *OI3iet_BO_TKULAe.H T CPMMkWMrtta'l.`tCX>'1tlAOaaW.'tMU.IMW*U(T*CnrSAWiOwratHWODanm"V :| S a a p lt ta d II >. Sanpt* Typ* ; Concuntcaticm Olank 0.00 up a . i . / t C a lc u lated Conci */A Ac. Oat*: i Ac. Timi 04/21/01 O TtlO iO l .504 >lo ltu i h ld i I! Al4 T b to h o ld l ' Ifua. SM Ottl: I n r mndowi tx p a c tt d ATI U p . N ldthl Sap. Rcsphtl lip , r u t M tlO! li l i p . M l . AatiO t j flip I v i. lu rio i j: VJ* Mlitlv ATI I 0JO.O II. 0.20 0.01 0.00 a)..o0o0 0JXW4 0-30*4 j 00*4 7 60*4 ' 700*4i 4AM MatpAtt ita c i T iM i And 7 tM i -87- Project No.: 112E-108 jJ 00*4 J IOM U O i4 200*4 Printing Date: Friday, April 22,2005 17 4. 7J0 4 7 1 04 11>I- n o m e U.aOtiJO I f I f 14 IS Figure 42. Representative ion chromatogram for PFUA in the soil extract from a Day 0 untreated control soil by LC/MS/MS. PFUA was not present (no peak at 11.8 min). Company Sanitized. Does not contpin TSCA CBI. W ildlife International, Ltd. -88- Project No.: 112E-108 ndlife L td . MSt> S a t i a i ti. FAXe s op:oato;r * Xr\tSCM Sl\ 1 \DATA\AI r-MZ4-1 VO6-01010 >D dw ....................... fiwfepi JSddlii.redi 18 A f t 107 3:0 f rn a e tu a ia n t: <stsrtMi ' #a$fXj3| Ndmes 1S92VXE3C Miae i n f o : 1 l^E -ios/lG Sy iio V ia l Sumbea? : 6 aU A U ttJ& Ji' Figure 43. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Qg cartridge extract) from a Month 24 untreated control soil by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -89- Project No.: 112E-108 Figure 44. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS. 8-2 FTOH was not present (no peak at 4.18 min). Company Sanitized. Does not contain TSCA CBI. Wildlife InternationalLtd. -90- Project No.: 112E-108 Figure 45. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 untreated control soil by LC/MS/MS. 7-2 sFTOH was not present (no peak at 11.9 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -91 - Project No.: 112E-108 Figure 46. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace 8-2 FTA at 12.3 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -92- Project No.: 112E-108 Figure 47. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 untreated control soil by LC/MS/MS. 7-3 Acid was not present (no peak at 11.6 min). Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -93- Project No.: 112E-108 Figure 48. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. 8-2 FTUA was not present (no peak at 12.3 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -94- Project No.: 112E-108 Figure 49. Representative ion chromatogram for PFOA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFOA at 9.91 min was <LOQ. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -95- Project No.: 112E-108 Figure 50. Representative ion chromatogram for PFNA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFNA at 10.7 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -96- Project No.: 112E-108 Figure 51, Representative ion chromatogram for PFDA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFDA at 11.4 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -97- Project No.: 112E-108 Figure 52. Representative ion chromatogram for PFUA in the soil extract from a Month 24 untreated control soil by LC/MS/MS. Trace PFUA at 12.0 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -98- Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: C:\HPCHEM\l\DATA\ALFI005\0801012.D Operator: fj1 Date Acquired; 30 Apr 105 4:29 pm Instrument: GCMS#2 Sample Name: 1 7 1 9 -TEX Misc Info: Vial Number : 8 Figure 53. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C|8cartridge extract) from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -99- Project No.: 112E-108 Figure 54. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 100- p. 100 Project No.: 112E-108 Figure 55. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -101 - p. 101 Project No.: 112E-108 Figure 56. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 102 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 S ^ N i! rw :>'i p l M i* F I * :*4105_D0_TELALAlL'lf' P o lH t fi :` PPOAC-7 M tw (et) *4130OM enV Cammed *112S-109,lC0.110<*PR<Er S n i p ) * Codaxs 1) S ta p le Types C eneentretiem QC 10.0 ug * . ./! 100(0 C a lc u lated Cooes 9.1702 ug a . i . / k g Acq. D ate: A cq. TIFiei 04/21/0S . 0 ? s3 1 i2 7 PM 9.90(4 i K e d l f le d ; He - lunching F aeton N o i `I h m h a l d : 1 9 9 .7 7 epa ' Ax T hzesheld: i Ho b . S tso o th n 194 9 .9 4 epa (' RT Window: B x p e cted KT: 30.0 aee 9.92 *ln j p . w idths 0 .2 9 $#p. H eight: 0.01 exp. Peek Ratios 4 .0 0 Il Cxp. A d ). R e tto ; 4 .0 0 ,[ e x p . V a l. R a tio s 1 .0 0 C ",M`" ND I n t . Types Rase To B ate I) R e te n tio n T in ts 9.91 n ln ! A re a: 170090. counts .> H eig h ts 29273.54469 epa : S ta r t T ints end Tines 9.67 Bin 10.0 a in 900(4 30t4< ooa4 ; 7 50*4 700*4 60*4 | 00*4 | 8.90*4 I 1.00*4 f 460*4 | 400*4 3 80*4 | 3.00e4 | 250*4 200*4 1.50*4 1,00*4 - 102- n 12 13 14 1 Figure 57. Representative ion chromatogram for PFOA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 103 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -103 - A **ha*'*`C-OA*W Ceavnarfc *112S*10>,tM>1 la a p l* Induxs i; S*npl* Typar IS CC 1 C oncentration! 10.0 ' C a lc u la te d Conct 9 .1 (0 * v a .i ug a .l ft. wiM.M.wos'jr- ******* ~ t .OM .' 5.90*4^ Hcdlfladt lunching fa c to i: Not* Thra*heldi 'At* ftit*ah*ldi | Mum. Snaethai IT Vlndevi Capictad H i Sep. W idth: lap. H aighti Cap. peak l a t i a i Up. Ad). Hanoi tap. V at. la tlo i Via talatiwe RTi 10.0 10.* 0 .2 0 0.01 (.0 0 4.10 1.00 if <p* (At. lype: ' Ratantlon Tinai 10.( in At**! 2 2 1 1 (0 . count* Might! )(((.99(1* cp* start final 10.4 nin Cnd Tinai 1 0 . 0 nln 00*4 I t 0.00*4 r.SO4 7.00*4 190*4 000*4- S99014' | 500*4- * 4SO*4j 390*4' 100*4 250*4 100*4 140*4 1.00*4 000 0J1 0 7 0742 Projectno.: 112E-108 Figure58. RepresentativeionchromatogramforPFNAinthesoil extractfromaDay0 fortified (250 pg8-2 FTOH/kgand10.0 pgPFCAs/kg) control soilbyLC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -104- p. 104 Project No.: 112E-108 Figure 59, Representative ion chromatogram for PFDA in the soil extract from a Day 0 fortified (250 |ig 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 105 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. marni --amm_yna * * i; P**>kkHi uMmn*:'*<eM.i1 Aw*P Ulli*i*M(*ti>it:l"SlU*XWOfl1l.0O.0 *muf' i 1 C*'*'*nC' t 1211010*.11Pt#PiK2;r A nne ****:" > Index: IS l i w l t lyp*: AC ,,, C w cenctatleni 10.0 up . ./l i C e lc u letad Cenci 9.1194 uy 4. 1 . rig 100 Ac. W t* A */t/iK Medi l u d i ktraM ng P a c ieri Mele* T K ra ah a ld t Afe* th re s h o ld s Jfua, te e e th i: T indewt Espected Rti S*p. W ldthi l* p . K elghti Cip. Peak n a tio i tx p . Ad), lu t ic i ! Bxp. V al. A etiei Use e l a t i v e M i N 1 >14.01 570.14 1 )0 .9 11.0 0 .2 0 0.01 5 .0 0 4.00 3.00 Ke ep Cp sic in I n i . Type d e te n tio n Tlstei A ceti 1 HeipM i ' S te it TIM I 2n d T la ti 0*s* Te I m i II. ain 19M70. count 1231C.022S < 11. aln 12.0 atri >0M] 50*4 ' 00*4 I; s o m - 1.00*4 1 10*4 00*4 ( >M*4 f **4 * 4 50*4 tllj 4.00*4 .i 150*4 [i li 3 0 0*4' 2 50*4 1 200*4 i I 0*4 ' 100*4 sonooo* -105- 11.14 01*.1 TOP POI ProjectNo.: 112E-108 Figure 60, Representative ion chromatogram for PFUA in the soil extract from a Day 0 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 106 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -106 - Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: T:\DATA\ALFI025\0801012.D Operator: DW Date Acquired: 20 Apr 106 8:04 pm Instrument: GCMS#1 Sample Name: 1735-TEX Misc Info: 112E-108,109,110 Vial Number : 8 Figure 61. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Qg cartridge extract) from a Month 12 fortified (250 jig 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -107- p. 107 Project No.: 112E-108 Figure 62. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Trace 8-2 FTOH at 3.98 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -108- p. 108 Project No.: 112E-108 >' q Li* I.* g a 1 >1 B *1 in * ] , p~ T" 'f.- --r "T 7--------- 5" Figure 63. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 109- p. 109 Project No.: 112E-108 Figure 64. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI W ildlife International, Ltd. - 110- p. 110 Project No.: 112E-108 Figure 65. Representative ion chromatogram for PFOA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -111 - p. 111 Project No.: 112E-108 Figure 66. Representative ion chromatogram for PFNA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -112 - p. 112 Project No.: 112E-108 Figure 67. Representative ion chromatogram for PFDA in the soil extract from a Month 12 fortified (250 pg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -113 - p. 113 Project No.: 112E-108 Figure 68. . Representative ion chromatogram for PFUA in the soil extract from a Month 12 fortified (250 |xg 8-2 FTOH/kg and 10.0 pg PFCAs/kg) control soil by LC/MS/MS. p. 114 Company Sanitized. Does not contain TSCA CBI. W ildlife InternationalLtd. Project No.: 112E-108 - 114- Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: C:\HPCHEM\1\DATA\ALFI005\1201020.D Operator: fj1 Date Acquired: 30 Apr 105 7:04 pm Inst rument: GCMS# 2 Sample Name: 1665-TEX Misc Info: Vial Number : 12 Figure 69. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C,8cartridge extract) from a Day 0 sterile soil treated with 200 mg/kg(, }by GC/MS. 8-2 FTOH was not present (no peak at 5.0 min). Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -115- p. 115 Project No.: 112E-108 Figure 70. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 sterile soil treated with 200 mg/kg[_ J b y LC/MS/MS. Trace 8-2 FTOH at 4.09 min was <LOD. p. 116 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 116- I KMii p* k N M :* -2 tt r HnMtKfiCRGRar- '4 T ro rm o * m u * Canwpat *1t-lCO-* Annotato* ** lu p i ]M i ~~ S a a fla Typai (M C M U itlM I .. C a lc u lated Cenci 9.000Q up a . l . / k q ., AAceq.. TPiaatati O04V/i 2101 /:r3*1 I M adlliodt Bunching r a c k e t: H oii* rtoraaheld: A lt* Ttireahold: . m a. liN tM i BT WlAdOw: B ipected BTt . ta p . Width, lap . H eight: Cap. Teak H atJoi ta p . Ad), BaUoi tap. Val. tie: ,11a* R e la tiv e Ti Mo 1 24.04 130.21 1 10.0 12.2 0.20 0.01 S.00 4.00 ).O0 Na epi epa tie bid aooo.ro aeoo.co aooooo raoo-ooj 7000 oo j 'I IM . ty p a r A ttendee tlB as Atea: ' H alfnu 1 s ta r t TIMI I EM T IM I Ho reek w/ a M/A w/ a M/A X/A aln count! epa Bin Bln il soooo- 00000- 1WOOS J aooooo; 1 asoooo; 4000.00 aacaoo 200009 Project No.: 112E-108 too 040 t Printing Date: Friday, April 22,2005 -------, ------- 22 004 , . . ft ' o" 7.00.7.04t - t s a i t ^ 0 *1 7 " T io t 1 1 1 * . 1100 n ' it ' Vo'""Ja is ' ' m ' i i S ' Figure 71. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS. 8-2 FTA was not present (no peak at 12.2 min). p. 117 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 117- ( " I T SaiW N*r i ' \ `i t n y k t j l 14' SairpU lD M ini* I 5 Peek Mam*'*$-3(Uf* Meeafsa). *497.0093.0Ml* ' Cam* *113E>t06-* A ftM ttton" Sin*) Index: 26 l i i p l i Type: Unknown o.ooooC e n c a n t t a t i e n : C a lc u lated Cone: N/A uq a .l./k g t.OQaS Acq, O at*: Acq. T ina: 0 4 /2 1 /0 5 0; 16:11 PM 9 6044 M oditied: unehtng r a e to rt H ols* T tuahold: A rea T h rtah e ld : Nu b . S a o e t h a : AT W indow: B xpactad *Ti Sap. w idth: S*p. H eight: Bxp. Peak A atio : 6x p . A d}. R a t i o : Bxp. V at. R atio: V t* R e l a t i v e AT; NO 1 201.61 11 0 0 6 .(4 3 0 .0 12 .1 0.20 0.01 5 .0 0 4 .0 0 1.00 No *ec Bln 0 00e4 6 90e4 8KM 7 60e4 7 OQM Ilnt. Type: P e ta n tio n Tib *; A rt H aight 1 !| S t a t e T i n t ; ! tn d Timet N/A N/A N/A N/A N/A m i) c o u n t* ep* n ln a ln 6S0M 6.00*4 i! ; f 6.00*4 * if $60*4 3 00*4 ISOM 2.00*4 1 60*4 6000.00 I 0.00 ' Printing Date: Friday, April 2 2 ,205 2 4 S 12 u ' * * ,7 -lf 612 r 7 t .t0.12 11,16 H J 6 1333. 1363 __________________ to it ili t i ' I S T Yi ............................................Tm .m in Figure 72. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg by LC/MS/MS. 8-2 FTUA was not present (no peak at 12.3 min). p. 118 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. ' Cm w m I126.10*-' i l u v t i 2nd*: 20 1 Saapla Typai Vntnown C M C tnm tlen: KM If C a lc u la te d cortet 0 .4)021 U t , Oat*l 04/21/01 T llM t 6 illi2 l HH edirtad: | StMchlng fa c te rr H Kota* T h tash v ld t [| Aca t h m t o i d : . IHM IKtl if fit V ln d w i |i farpactad KT: l| sa p . w idth! ' ta p . Height! Cap. Paak k a tlo i Cap. Ad). M tlo l : Cap. V al. R atiet . tf*a R e la tiv e P it X | HatgHti I state u m i ' end TIM! IM 7.*S4I epa - 118- Project No.: 112E-108 S9IW4 I 900*4 250*4 | 100*4 eooaooj 000-1----- Figure 73. Representative ion chromatogram for PFOA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg "jby LC/MS/MS. Trace PFOA at 9.81 min was <LOQ. p. 119 Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. -119- r' &ni pi P**kNm* 'C-OAM* HiM<(.,490M1fOam* e w u M M 'I IK - IM - ' Ah m S I m " Saapla Indexi 20 : 34Spl* tVP*l Unknown , C enetatcation: MM ' C a le v lo to d Cone: 0.0000 ug 4 . i . / k g ' Acq. m o t Acq. TlM I 0 4 /2 J/O S 0 il( 3 1 0M SQM M ediriad: Bunching E a c tsri Mokao T l u i i h i d i Ai m 7 h r a h o ld i Hus. S a o e th u *7 Window: k e p a c ta d HTt Sp. w idth) i Sp. H aight: Exp. N ik M tio i Exp. M j. M e ist Exp. V 4l. M tio i W*s M l a t i v s T: No 102.01 0(4.25 30.0 10.< 0.20 0.01 S.00 4.00 3 .0 0 HO P cpl in (O M SOM eoM ,I tn t. Typ: M tan tio n T isat A tsai H aight: S taat T isat EM T ise: No Pool MM HM HM MM NM in e s tin ta epa ln ln d t e Iala e Jr Project No.: 112E-108 StOM ; sin ; 100*4 ; sooooo- Priniing Date: Friday, April 22,2005 Figure 74, Representative ion chromatogram for PFNA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg(. Jby LC/MS/MS. PFNA was not present (no peak at 10.6 min). p. 120 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 120- T P u li N M 'X -10Add* W*a*<t) 'St3(V480*w* C**mtfi`m e -tO * -' Aonot***-- liip ) tM i; 24 Soapio Typ*i Unknown c * n e n tr tie n t W/A i-OOo) C o l e u la ttd Conci 0 .J9T 4) 19 o . t . / k q Aeq. at> 01/ 21/ 0* Acq. r i u t m ! som K adK ltkl Aunehlnf Paertt Mafao h r a a h o ld : Ac* T heahold1 Mu, SM O thsi At Hindou1 I t p a c M AT1 Sap. W idthi Sap. Holqhti Sip. N ik Patiei t i p . Ad), Pott! C ip. V41. Potto! U*4 M lO tiV * At! 0. 0) 3.00 1.00 3.00 OOOat I 0 604 J 00*4 I 7 SO*4 I I 7.00*0 j K olqht! S t a r t Tlo: b id Tlmos 71*).7 count 122. 0*9 epo 1 1 .1 in 0.90* I i :90*4 00*4 >S0*4 300*4 300*4 200*4 . Project No.: 112E-108 Printing Date: Friday, April 22,2005 Figure 75. Representative ion chromatogram for PFDA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg^ J b y LC/MS/MS. Trace PFDA at 11.2 min was <LOQ. p. 121 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 121 - 1 S * ( ^ M a n * J3*npU j?4' $*mpltO. MOM* f H : 1>it j S T D u > r Reek N**: *0.11 A rif '$ 30419 0 t m f Cemment *112K-t0i^ A m h t n " S ta p le index: 20 S tap le TypI Unknown C oncentration: a/A C e lc u lated Cenci u .012040 uq e .t ACq. O a t* : 04/21/05 Acq. T la et 119:14:31 PM M odified: * Ourtehlaq r e c to r : j Mole* T h resh o ld : {-Aree T hreshold: 11 Wua. S a o e th : ' RT Window: Ko i 114.03 $ 7 0 .IS 1 30.0 ' E x p ec te d RTi 11.0 Sep. W idth: Sp. M elqht: 0 .2 0 0.01 Cap.' Peek R ecto: . Rep. A di. R e tto : $ .0 0 4 .0 0 lap . V ei. R edo: Use R e la tiv e RT: 3 .0 0 Ko 100*6; 060*4 000*4 ' 060*4 -j 600*4 760*4 700*4- ln*. Typ: R etention T int! Ar**< H eiqht: - s t a r t Ti m : , End T la e i ____ _______ _ 11.1 a in 1570.4 count* 541.43444 cp* 11.7 a la if .0 ain 60*4 00*4 600*41 460*4 < S 60*4 300*4 l( MM 7.00*4 100*4 i I 00*4 . Project No.: 112E-108 Printing Date: Friday, April 22,2005 Figure 76. Representative ion chromatogram for PFUA in the soil extract from a Day 0 sterile soil treated with 200 mg/kg *)by LC/MS/MS. Trace PFUA at 11.8 min was <LOD. p. 122 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -122 - Wildlife International, Ltd. GC-MS#2 MSD Serial No. 3118A02483 File: Operator: T:\DATA\ALFI025\1301021.D DW Date Acquired: 20 Apr 106 11:09 pm Instrument: GCMS#1 Sample Name: 1682-TEX Misc Info: 112E-108 Vial Number : 13 Figure 77. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (C|gcartridge extract) from a Month 12 sterile soil treated with 200 mg/kgC Joy GC/MS. Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -123- p. 123 Project No.: 112E-108 Figure 78. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -124 - p. 124 Project No.: 112E-108 Figure 79. Representative ion chromatogram for 8-2 FTA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg_ J b y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 125 - Project No.: 112E-108 y . iw yntw w ag,, T> f JT Figure 80. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 12 sterile soil treated with 200 m g/kg Jby LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -126 - p. 126 Project No.: 112E-108 Figure 81. Representative ion chromatogram for PFOA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg^ }>y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -127 - p. 127 Project No.: 112E-108 Figure 82. Representative ion chromatogram for PFNA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg(^ by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -128- p. 128 Project No.: 112E-108 Figure 83. Representative ion chromatogram for PFDA in the soil extract from a Month 12 sterile soil treated with 200 mg/kgC J b y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 129- p. 129 Project No.: 112E-108 Figure 84. Representative ion chromatogram for PFUA in the soil extract from a Month 12 sterile soil treated with 200 mg/kg J by LC/MS/MS. p. 130 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 130 - Wildlife International, Ltd. GC-MS#2 MSD Serial No. 31I8A02483 File: C:\HPCHEM\l\DATA\ALFI005\1001014.D Operator: fjl Date Acquired: 30 Apr 105 5:06 pm Instrument: GCMS#2 Sample Name: 1593-TEX Misc Info: Vial Number : 10 Figure 85. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Cl8 cartridge extract) from a Day 0 live soil treated with 200 mg/kg_ j b y GC/MS. 8-2 FTOH is not present (no peak at 5.0 min). p. 131 Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. -131 - Project No.: 112E-108 Figure 86. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Day 0 live soil treated with 200 mg/kg 3 by LC/MS/MS. Trace 8-2 FTOH at 3.93 min was <LOD. Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. -132- p. 132 Project No.: 112E-108 Figure 87. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Day 0 live soil treated with 200 mg/kgj? ^by LC/MS/MS. Trace 7-2 sFTOH at 11.9 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. I"1 I P"aiik" M imai"*"M0.2<(SS|*r lMiaasa*<t)---'4--7--7--0--9--0-3--0-/* Cmrnnr 't l t E - t n - ' Awwbtoev* Saapl* Iodxi 17 BSCCM*ueaosnnlqdcdciurlla*iilnncagdTttceiyadrptaicaeiont on: er s: (feknevn V/A <0 ;t ANAAtoeeqqt,. * TTt ul;hhmtt*rtali h h ld olds t 04/21/05 0 8 :1 1 :1 0 1 Mua. SaoothS I I- xt Windows 20.0 Itx p c t d RTs 12.2 |, s p . Width: 0.20 I Sp. H aight t 0.01 I Cap. f*K R a tiu i |s X- A d ). R a tio : 1 e|>. v a l . R a tio : 5 .0 0 4.00 2.00 ' Us R l itiv XT: m I n t . Typos A tntioo Tim A raa: Haight* S ta r t TUw: End Ti m s Rasa To la s 12.2 a ln I 72. l l count* 142.08282 cp* 12 .1 airs 12.4 a in ^SOOOOO - 133- p. 133 Project No.: 112E-108 100000 too 00 000 Figure 88. Representative ion chromatogram for 8-2 FTA in the soil extract from a Day 0 live soil treated with 200 mg/kg *]by LC/MS/MS. Trace 8-2 FTA at 12.2 min was <LOD. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 134- p. 134 Project No.: 112E-108 Figure 89. Representative ion chromatogram for 7-3 Acid in the soil extract from a Day 0 live soil treated with 200 mg/kg ]by LC/MS/MS. 7-3 Acid is not present (no peak at 11.6 min). p. 135 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -135- fir.`.' H ^'^ "W irne: `limpi* Oil* M yfcSPTSV '04StW_06_TiLM> w r ' '< P*k Nmiw: *0-2 [ \if Mm ) *457.0/3 0 mu* . C*mm*At*112E*10-* A iw W iit ` S aapl* tnd**i 17 * * ! Type Unknown C encentratlont M/A 100* ; C a lc u la t e d Cofici 0.0000 0 4 .1 |A eg. M tti 1Aeg. Tin* i 04/21/0 00:141 IS PH ttM ' H editlad: Bunching Factor 1 W ei T h z c ih e ld i ! A r t thc*hlfl: j Hu*. S*e*th*t XT Windowi expect n il Sep. W idthi 3*p. H eightt Exp. Peak R atloi Exp. Ad) . R e tie : Exp. V al. R atio: ; Ua* R e la tiv e RTl Ke 1 201.03 1000.64 1 3 0 .0 12.3 0.20 0.01 3 .0 0 4 .0 0 3 .0 0 Ke ep* cp* In 00*4 050*4 I I000*4 7 90*4 700*4 I n t. Type; X atcn tio n T inei A re : m ig h t: S tilt tin * : End Time: Ke Peak K/A M/A K/A K/A K/A in ceunt* cp* in in 060*4 I000*4 J 830*4 .1 |!i I l! ) 00*4 j 960*4 j 100*4 , ii 100*4 ' 100*4 I 600000 j 000 a ' 4 S 0 T Project No.: 112E-108 Figure 90. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Day 0 live soil treated with 200 mg/kg Jby LC/MS/MS. 8-2 FTUA was not present (no peak at 12.3 min). p. 136 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 136- I S*m*t*Nm* t i i i p l a J M I ' P h INi m pfOAC-*' Co m m i* '112&10B.' AnnoMan-** II S a n p l* In d a * : 17 3 u ^ l Typat Unknown j| C o n c e n t r a tio n : )t/A I C a lc u la te d Cone: 0.CS3IS ug a . l . / k q j| A ceq . TDIamtai; 0 4 /2 1 /0 $ PM Modi f t a d : Bunching fa c to rs 'I N o iaa T h r e s h o ld : Area T hresholds , Mia*. taMthii RT M lndovi ` capected Rti f J c p . Width! 1' S ap . H a ig h t: Bap. Saak R a tta i ta p . Ad}. R a tio : I U110.0 0.10 0.01 $.00 4 .0 0 3 .0 0 ,i i n t . T y p at ' R e te n tio n Ti m i Ar e a : H aighti S ta re T ina: End n n a i B ast To Baa* * .0 J min 14347. counts 24BO.$ 0297 epa .70 Bln 10.0 min F i t : *042106 00 TEUtlAE.vSP'* `i 00*4 ; .00*4 B.S0*4 00*4 ' 7.BO*4 : 7.00*4 1 A00*4 | j 00*4 | s^i It * 6 0 * 4 . ( 400*4-; i. 3.90*4 2.60*4 200*4 190*4 1 00*4 6000 00 Project No.: 112E-108 11 12 1 14 Figure 91. Representative ion chromatogram for PFOA in the soil extract from a Day 0 live soil treated with 200 mg/kg _Jby LC/MS/MS. Trace PFOA at 9.83 min was <LOQ. p 137 Company Sanitized. Does not contain TSCA C8I. W ildlife International, Ltd. - 137 - l| h Barn! , _ '.TM. U6mj- h..itiiMibbjiwiMJtir I PnkN nw .*C4A cW *4]0/41 9 .0 **W I CemmtnC *1l2C<109-' Ah m M m :'* S tapl* Inda: 17 SM pla Typai unknown II c o n c e n t r a t i o n : N/A *00*5 II c a l c u l a t e d C onci 0 .1 7 9 1 6 u g a . l . / k g !| A eg . D aki Aeq. 1 \m : 0 1 /1 1 /0 $ B i U 1 : l l PM 960*4 IM odified: Bunch!*? F a c to r: M ols T h resh o ld : M n T k tttlw ld : Mun. SBOOths: No 3 0 .0 10. 6.10 0 .0 1 AT Window: E xpected n r: Sap. W idth: Sap. H aight: No l| c u p . Pak R e tie : 'I EI nx pt .. AT ydp;, : R a tio : B a sa TO B a t \ RKapu. nVt taoln. uP amt i o: : 1 0 .6 ln '' UA sree aR: a la tlV * RTl SS ! . 6 c e u n t s . H aight: 664.4(642 cp* S ta rt T ina: 10.4 Bin End T ln a t 1 0 .7 mxn 900*4 so* 000*4 760*4 700*4 9 $0*4 ; ' 500*4 4 60*4 4 00*-: 360*4 9.00*4 2.60*4 200*41.60*41.00*4- 6000.00 000 : Project No.: 112E-108 Figure 92. Representative ion chromatogram for PFNA in the soil extract from a Day 0 live soil treated with 200 mg/kg J b y LC/MS/MS. Trace PFNA at 10.6 min was <LOD. p. 138 Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. - 138 - W flllO MMT fta *M?1DB.M_TElAlACwfF I m m i m 'c -h /m M M r t i t M N t M i r Sa^t* In4i ) I ta p l i Typ> IktAM i ClIew litM CMi 0.41IH M i A'. M il l 04/2)/ U , Tll N lK l I I IH iM KUftcS! M tfMhlM r a ti o n i ;.ku* m.jo ; Ac** Thit>kUi IK.SO I rt viadowi I Ciprot<d RTl tp. Widths 'Jap. M lqhtl Kip. h i t 21.0 )).J 1.20 Il. T ffi! R ttcn L im Xmi H rlfh tl S titt riH i lu ll la Iim 11.2 MR 710).1 c m i< I2 H1.I1K.M fpi ua 00M DM ; I 90M | Project No.: 112E-108 UM 100M t4 00 1 2 o' 'i 5 0 ? 1* "to 1 'll "0 Figure 93. Representative ion chromatogram for PFDA in the soil extract from a Day 0 live soil treated with 200 mg/kg. J b y LC/MS/MS. Trace PFDA at 11,2 min was <LOQ. p. 139 Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. Project No.: 112E-108 -139- I >*mpUNam*'*6*>nf4*J>t 1T empi* 10 *190T Ffc:W Jt05_t_TeiA LA.T?^ Peak Nim*: *0-1 t Ackf U*<*>: *66* H M .0 amW Annctafan: " saapla lnd*si 17 Saepl* ty p t Unknown C oncentration! N/A 100*5 C a lc u la te d Contst 0 .0 0 0 0 ug a . l . / k g Acg. O stai Act). T in o t 0 4 /2 1 /0 0 O O iltilO OK 0 BON H editl*d! j Owvchlng F a e t o n || N oise T h resh o ld ! * i T hreshold: | Ntao. S n o o th a i RT Window! E x p e c te d RT: I Sap. W idths I sap. H eight: I Exp. Peek R atto; I Exp. A d). R a tte t I Esp. V al. R atte! Ose R e l a t i v e RT: 3 0 .0 11.0 0.20 0.01 5 .0 0 4 .0 0 3 .0 0 He 300*4 tS M 1 0.00*4 j 7.80*4- 700*4 tn t. Typ*t R eten tio n T ine: A rsa: ! H eight; S ta r t T i**i ' End T tn * i Ne Peak K/A N/A N/A N/A N/A in count* epa Bln Bin 00*4 a 9.00*4 8 6.00*4 * 4.80*4 400*4 ^ 160*4 3.00*4 2.60*4 100*4 140*4 1.00*4 mwkfw 000 1 * 3 4 $ 6 f 6 B 0 n 12 t3 l i 16 1 " l > * 7 1 iL _____________ _____ . _ Jn y e . min ,, Figure 94. Representative ion chromatogram for PFUA in the soil extract from a Day 0 live soil treated with 200 mg/kg(_ ^ by LC/MS/MS. p. 140 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 140- Project No.: 112E-108 W iId -life Infceamati.ona1 , lit# . MSD' S e f i & l NO. m eir xj >jpe*a to x ? 43W . P ete A c& y ife d i 18 A p t 10V 10* trusted tsr S G M S ll Sntpie Haste t Mia Ir tfo : V i s l Nsuofeei? 7 3:28 pat 1618-THSt llS fE -lO S U il&A6ii4&:3 i.--i--| i*"t > i | i. > i `i | v * |--i--i--i-- i--i--i `1 r -r t 'i1 i i "v - I - i --i i i- . i 4 . 4 0 A4 . 6 6 4>80 3 . 0 0 S . g.Ql g.&Q & j&Q B , S 0 Khantfaisee 153 a s . 00 134,70 to 9 5 .7 0 )5 0 7 0 1 p ll. ` .'2$pISO - | rime.--> .4,..'40' A - i ' | , i |. 1 j t i Figure 95. Representative ion chromatograms for 8-2 FTOH in the sampled headspace (Ci8cartridge extract) from a Month 24 live soil treated with 200 mg/kgC J b y GC/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -141 - p. 141 Project No.: 112E-108 Figure 96. Representative ion chromatogram for 8-2 FTOH in the soil extract from a Month 24 live soil treated with 200 m g/kgt }by LC/MS/MS. Trace 8-2 FTOH at 3.96 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -142- p. 142 Project No.: 112E-108 Figure 97. Representative ion chromatogram for 7-2 sFTOH in the soil extract from a Month 24 live soil treated with 200 mg/kg J by LC/MS/MS. Trace 7-2 sFTOH at 11.9 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 143 - p. 143 Project No.: 112E-108 Figure 98. Representative ion chromatogram fmr 8-2 FTA in the soil extract from a Month 24 live soil treated with 200 mg/kg Jby LC/MS/MS. Trace 8-2 FTA at 12.3 min was <LOQ. Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. -144- p. 144 Project No.: 112E-108 Figure 99. Representative ion chromatogram for 7-3 Acid in the soil extract from a Month 24 live soil treated with 200 mg/kg(f J b y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -145- p. 145 Project No.: 112E-108 Figure 100. Representative ion chromatogram for 8-2 FTUA in the soil extract from a Month 24 live soil treated with 200 mg/k$ J by LC/MS/MS. Trace 8-2 FTUA at 12.3 min was <LOQ. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -146- p. 146 Project No.: 112E-108 Figure 101. Representative ion chromatogram for PFOA in the soil extract from a Month 24 live soil treated with 200 mg/kg J b y LC/MS/MS. Note: Secondary dilution required and reported for PFOA. Original analysis was over calibration range. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 147- p. 147 Project No.: 112E-108 Figure 102. Representative ion chromatoeram for PFNA in the soil extract from a Month 24 live soil treated with 200 mg/kgC . J by LC/MS/MS. Company Sanitized. Does not contain TSCA CBI, W ildlife International, Ltd. - 148 - p. 148 Project No.: 112E-108 Figure 103. Representative ion chromatogram for PFDA in the soil extract from a Month 24 live soil treated with 200 mg/kgC J y LC/MS/MS. Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -149- p. 149 Project No.: 112E-108 Figure 104. Representative ion chromatogram for PFUA in the soil extract from a Month 24 live soil treated with 200 mg/kg J>y LC/MS/MS. p. 150 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 - 150Appendix I Measured Concentration of Selected Analytes (ng/kg Soil Dry Weight) p. 151 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -151 - Project No.: 112E-108 Wildlife Intematimal, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (pg/kg) in Soils Treated with f j Sam ple A erobic A naerobic S o il Type_________ Interva l_____________L ive S terile_______L ive S terile Uliisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 17.8 19.1 32.0 36.5 57.8 61.4 122.1 150.0 269.7 373.8 376.4 441.1 487.0 558.6 367.7 344.1 340.1 362.6 410.7 328.7 365.9 329.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 <LOQ 2.4 <LOQ 2.2 <LOQ 4.9 <LOQ 7.8 <LOQ <LOQ <LOQ <LOQ <LOQ 21.7 22.7 26.8 23.9 51.5 40.3 70.0 73.3 114.7 133.5 148.6 104.7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.5 <LOQ 2.8 <LOQ LOD = limit o f detection (0.206 ng/kg) LOQ = limit o f quantitation (2.0 pg/kg) Thursday, November OS, 2007 p. 152 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -152- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations ofPFOA (pg/kg) in Soils Treated with Soil Type Sam ple In te r v a l A erobic L ive Sterile M ollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 13 Month 18 Month 24 <LOQ <LOQ 15.4 15.1 17.8 18.0 21.7 20.2 31.8 30.3 46.0 58.6 74.7 74.4 92.5 69.3 103.8 94.0 98.5 247.9 126.9 118.8 229.3 207.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.9 <LOQ 3.2 2.3 3.4 3.3 3.0 3.1 LOD = limit o f detection <0.206 /ig/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile <LOQ <LOQ 14.7 15.1 18.8 18.1 20.2 22.3 42.8 43.2 78.5 61.5 117.4 91.0 . <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.7 <LOQ 3.6 2.4 Thursday, November OS, 2007 I p. 153 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -153 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (ug/kg) in Soils Treated with J Sam ple A erobic A naerobic S o li Type_________ Interval_____________L ive Sterile_______L ive Sterile Alfisol _ DayO Week 1 Week 2 Week 4 . Month 2 Month 4 Month 6 Month 9 Monti) 12 Month IS Month 18 Month 24 <L0Q <L0Q 8.5 8.2 11.4 11.0 14.9 15.8 23.2 25.0 32.0 33.1 58.4 52.5 97.9 93.7 65.4 84.2 133.1 103.5 194.4 155.9 196.5 190.7 <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3.3 <LOQ <LOQ <LOQ <LOQ <LOQ 9.4 10.9 14.1 15.4 19.7 19.8 17.9 31.1 90.0 77.8 99.3 83.5 <LOQ <LOQ <LOQ <IOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 <LOQ 2.2 <LOQ LOD = limit o f detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Frida,, October 12, 2007 p. 154 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 154- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFOA (ug/kg) in Soils Treated w itt 3 Soil Type Sam ple In te r v a l A erobic liv e Sterile A naerobic L ive Sterile InceDtisoI Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 18.9 20.8 28.6 29.0 55.7 54.7 122.2 97.7 190.4 189.4 264.7 250.4 288.5 339.5 367.8 350.0 402.3 385.3 422.5 446.5 530.9 554.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.1 <LOQ 2.8 <LOQ 4.4 <LOQ 5.6 3.0 6.2 2.8 4.2 2.5 <LOQ <LOQ 20.9 17.7 26.5 26.8 75.5 48.1 138.0 144.3 284.5 302,6 327.0 347.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 5.0 <LOQ 7.6 <LOQ LOD = limit of detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Frida,, October 13, 3007 p. 155 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -155- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA Oig/kg) in Soils Treated w ith^ 3 Soil Type Ultisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 A erobic L ive Sterile <LOD <LOQ <LOQ <LOQ 2.1 2.5 4.0 3.2 6.3 6.7 12.3 12.7 12.1 19.3 30.9 19.7 6.0 7.6 11.3 13.5 15.6 6.4 15.6 32.3 <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD LOD = limit of detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile CLOD CLOD CLOD CLOD CLOQ cLOQ CLOQ CLOQ CLOQ CLOQ cLOQ CLOQ cLOQ cLOQ CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD Friday, October 12, 2007 ! p. 156 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -156- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-1Q8 Measured Concentrations of 8-2 FTUA (fig/kg) in Soils Treated withJT ^ Soil Type Mollisol . Sam ple In te r v a l Day 0 Week l Week 2 Week 4 Montli2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <L0Q <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ 3.0 <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 gg/kg) A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ CLOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD CLOD Friday, October 13, 3007 p. 157 Company Sanitized. Does not contain TSC CBI. W ildlife International, Ltd. -157- Project No.: 112E-108 Wildlife InternationalLtd, Project N um ber 1 12E-108 Measured Concentrations of 8-2 FTUA (pg/kg) in Soils Treated with Soil Type Alfisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 13 Month IS Month 24 A erobic L ive Sterile CLOD <LOD CLOD <LOD <LOD CLOD cLOQ cLOQ cLOQ CLOD CLOD CLOD cLOQ CLOQ cLOQ cLOQ cLOQ cLOQ CLOQ CLOQ cLOQ <LOQ cLOQ cLOQ <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOQ LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile CLOD CLOD CLOD CLOD CLOD CLOD CLOQ CLOQ CLOD cLOQ ' 3.9 2.2 2.3 cLOQ CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ CLOQ Friday, October 12, 2007 p. 158 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -158- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (jrg/kg) in Soils Treated with Soil Type Inceotisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 A erobic L ive Sterile <LOQ <LOQ <LOD <LOD <LOQ <LOQ 5.9 5.0 25.2 12.3 77.7 88.9 164.0 160.4 192.1 161.0 209.5 206.9 225.6 252.1 256.2 242.7 248.5 239.0 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2.7 <LOQ 2.2 <LOQ <LOQ <LOQ LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile <LOD cLOD <LOD <LOD <LOD <LOQ 7.7 <LOQ 5.6 5.5 13.0 13.0 15.4 10.0 <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD cLOQ cLOQ Frida,, October 11, 3007 p. 159 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -159 - Project No.: 112E-108 Wildlife International, Ltd,___________________ ProjectNumber 112E-108 Measured Concentrations of 8-2 FTA (ng/kg) in Soils Treated with 3 Sam ple A erobic A naerobic S o il Type_________ Interval__________ L ive Sterile_______l iv e Sterile Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD <LOD <LOQ <LOQ 3.8 3.7 6.2 4.7 8.6 8.5 9.7 8.0 10.1 19.0 19.7 12.0 3.0 4.4 4.3 3.8 6.7 2.9 4.7 5.2 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD cLOD cLOD CLOD cLOQ CLOD cLOD CLOD cLOD 2.0 cLOD cLOD CLOD CLOD CLOD CLOQ <LOD cLOD CLOD cLOD CLOD CLOQ cLOQ CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD LOD " limit o f detection (0.503 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 160 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 160- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTA (jxg/kg) in Soils Treated with!. Soil Type Mollisol Sam ple Interval . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <LOD cLOQ <LOQ cLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD cLOD <LOQ 2.3 cLOD CLOD CLOD CLOD CLOQ CLOQ CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD cLOD LOD - limit o f detection (0.503 pg/kg) LOQ = limit o f quantitation (2.0 (jg/kg) A naerobic L ive Sterile CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD <LOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD Friday, October 12, 2907 p. 161 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 161 - Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Soil Type Altisol ' Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated witta 3 Sam ple In te r v a l A erobic L ive Sterile A naerobic L ive Sterile Day 0 Week 1 Week 2 Week 4 . Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD cLOD <LOD <LOD CLOD <L0D <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ cLOQ cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOQ cLOQ cLOD CLOD CLOQ CLOQ CLOQ cLOQ cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOQ LOD = limit o f detection (0.503 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 162 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 162- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTA (pg/kg) in Soils Treated with_ 1 Soil Type Incentisol Sam ple In te r v a l . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <LOQ <LOQ <LOD <LOD <LOQ <LOQ 5.5 4.8 18.0 13.0 73.7 75.7 162.8 144.6 163.8 126.8 133.2 140.6 193.0 205.7 213.7 226.5 202.5 221.7 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 2.9 <LOQ 2.8 <LOQ <LOQ <LOQ LOD = limit o f detection (0.503 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile <LOD <LOD <LOQ <LOD <LOD <LOD 6.6 <LOQ 2.5 2.6 6.1 5.9 8.5 5.3 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ Friday, October 13, 3007 p. 163 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -163- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFNA (pg/kg) in Soils Treated with^ S o il Type Ultisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 A erobic L ive Sterile A naerobic L ive Sterile <LOQ <LOQ 7.0 7.2 11.5 12.3 17.6 16.7 24.0 21.5 28.0 30.0 28.5 30.7 33.2 30.1 8.6 15.7 23.8 26.2 29.8 24.4 31.0 29.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ . 6.5 7.0 9.2 9.5 15.7 13.7 15.4 21.5 23.4 22.4 30.0 25.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit o f detection (0.124 pg/kg) LOQ " limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 164 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 164- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Soil Type Moilisol Measured Concentrations of PENA (fig/kg) in Soils Treated with 3 Sam ple In te r v a l A erobic L ive Sterile A naerobic L ive S terile Day 0 Week l Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ . 5.8 6.0 5.7 8.3 10.6 12.3 18.1 17.2 22.4 22.5 21.2 20.0 17.3 11.1 19.5 18.4 14.6 25.8 20.7 17.1 25.7 34.7 <LOD <LOD <LOQ <LOQ <LOD CLOD cLOQ cLOQ cLOQ CLOQ CLOQ CLOQ cLOQ CLOQ cLOQ cLOQ CLOQ CLOQ cLOQ cLOQ 4.2 4.3 5.8 4.6 4.3 5.9 6.5 8.2 12.1 9.3 16.0 12.1 CLOD CLOD cLOQ CLOQ CLOQ CLOD <LOQ CLOQ cLOQ CLOQ CLOQ cLOQ cLOQ <LOQ LOD = limit o f detection (0.124 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 165 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -165- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFNA in Soils Treated withC Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive Sterile Alfisot Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ 3.3 4.0 4.6 3.9 6.6 7.7 10.4 16.2 10.6 13.5 8.9 15.9 17.6 23.3 13.4 17.5 23.1 15.2 15.7 17.7 25.3 22.0 <LOD <LOD <LOQ <LOQ <LOD CLOQ <LOQ cLOQ CLOQ CLOQ cLOQ CLOD cLOQ CLOQ cLOQ CLOD cLOQ cLOQ cLOQ cLOQ cLOQ 2.4 2.7 3.2 4.8 4.9 3.6 6.6 8.9 9.2 11.2 7.9 cLOQ cLOQ CLOQ CLOD cLOQ CLOD <LOQ cLOQ cLOQ CLOQ cLOQ CLOQ cLOQ CLOQ LOD = limit o f detection (0.124 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Frida,, October 13, 3007 p. 166 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 166- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E -108 Measured Concentrations of PFNA (pg/kg) in Soils Treated with(F S o il Type Incentisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <LOQ <LOQ 20.3 25.1 29.0 33.4 36.9 35.2 62.2 63.9 59.4 61.9 67.9 79.9 79.9 79.5 71.5 70.7 73.9 74.1 79.2 80.6 96.5 104.2 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit o f detection (0.124 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic liv e Sterile <LOQ <LOQ 9.5 7.2 13.1 13.5 34.9 21.8 24.8 26.6 28.7 35.7 34.0 36.1 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q Friday, October 12, 2907 p. 167 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 167- Project No.: 112E-108 Wildtife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFDA ftig/kg) in Soils Treated with J Soil Type Ultisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 LOD = limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A erobic L ive Sterile <LOQ <LOQ 4.6 4.8 7.6 8.4 10.7 11.9 18.0 18.5 26.6 28.2 30.4 35.5 37.9 39.3 9.2 20.9 33.3 34.0 48.1 36.5 50.7 44.3 <1.0 Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ 4.8 5.2 6.9 7.3 9.3 8.5 13.2 15.0 25.4 28.4 21.9 19.6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <1.0 Q Thursday, November 08, 3007 p. 168 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 168- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFDA (pg/kg) in Soils Treated with^ J Sam ple A erobic S o il Type_________ Interva l_____________L ive Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 3.4 3.7 4.7 5.2 6.2 6.2 9.9 10.1 14.6 16.4 17.3 17.6 16.6 12.4 19.7 18.6 14.6 27.7 22.0 21.4 45.4 48.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 <LOQ <LOQ <LOQ <LOQ 2.1 <LOQ <LOQ LOD = limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A naerobic L ive Sterile <LOQ <LOQ 3.7 3.7 5.4 4.7 6.5 6.7 11.0 10.7 17.4 14.2 21.0 17.5 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <l o q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Thursday, November 08,2007 p. 169 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 169- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFDA (jig/kg) in Soils Treated with ^ Soil Type Alfisol Sam ple A erobic A naerobic In terva l_____________L ive Sterile_______ L ive Sterile Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 M onth 15 M onth 18 Month 24 <LOQ <LOQ <LOQ 2.1 2.9 3.0 4.4 4.8 8.1 9.2 8.4 9.8 9.3 12.5 17.1 19.7 12.9 16.1 26.3 15.3 23.3 20.7 37.6 36.8 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ 2.4 3.1 3.6 5.4 5.6 4.8 8.7 12.9 13.2 14.6 12.2 <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ LOD = limit o f detection (0.114 pg/kg) LOQ limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 170 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -170- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFDA (pg/kg) in Soils Treated wiihQ ^ Soil Type InccDtisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 LOD " limit o f detection (0.114 pg/kg) LOQ = limit of quantitation (2.0 pg/kg) A erobic L ive Sterile <LOQ <L0Q 4.5 5.4 8.1 8.3 15.6 15.6 34.3 29.8 43.5 50.4 52.3 68.8 66.7 57.2 62.0 69.8 62.9 66.0 81.4 77.9 86.7 89.6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.1 <LOQ 2.6 <LOQ 3.1 3.1 2.9 2.3 23 2.0 A naerobic L ive Sterile <LOQ <LOQ 5.3 4.5 7.5 7.8 20.9 11.5 21.4 22.9 33.7 39,3 47.0 41.0 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.7 <LOQ Friday, October 12, 2007 i i ! p. 171 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 171 - Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFUA (ug/kg) in Soils Treated with J Soil Type Ultisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 LOD = limit o f detection (0.128 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) A erobic L ive Sterile <LOQ <LOQ <LOQ <LOQ 3.0 3.3 5.1 4.7 8,5 8.0 13.2 15.0 16.0 16.4 18.8 17.0 3.6 10.5 15.1 15.5 19.2 16.1 22.5 22.7 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ 2.7 2.6 4.1 3.9 6.9 8.2 15.5 13.1 18.2 17.6 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October l i , 007 p. 172 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 172- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFUA (jig/kg) in Soils Treated with ^ Sam ple A erobic A naerobic S o il Type ______ Interval_____________ L ive Sterile_______L ive Sterile Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month6 Month 9 Month 12 Month IS Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ 2.1 2.7 2.7 6.0 5.7 10.0 10.8 9.5 9.6 8.2 4.6 8.8 8.6 6.1 11.6 10.4 9.2 14.5 21.3 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 2.0 2.5 3.5 6.0 4.7 6.8 5.5 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD LOD = limit o f detection (0.128 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 i j p. 173 Company Sanitized. Does not contain TSC A C 8I. W ildlife International, Ltd. -173- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFUAJjug/kg) in Soils Treated with[_ J Sam ple S o il Type_________ In terva l Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month L2 Month IS Month 18 Month 24 A erobic liv e Sterile <LOD <LOD <LOQ <LOQ <LOQ <LOQ 2.0 2.3 4.6 6.1 4.1 6.2 3.2 6.2 7.3 11.3 7.6 9.2 13.5 5.8 7.0 7.2 14.4 10.1 <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ A naerobic L ive Sterile <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 3.0 3.1 3.3 4.5 3.5 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD <LOQ <LOQ LOD limit o f detection (0.128 pg/kg) LOQ " limit o f quantitation (2.0 pg/kg) I Friday, October 12, 2007 p. 174 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -174- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFUA (Hjjj/kg) in Soils Treated withC Soil Type InceDtisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 A erobic L ive Sterile <LOQ <LOQ <LOQ 2.4 3.9 4.3 7.1 6.0 19.0 19.8 18.7 18,8 20.8 28.0 ,29.4 28.2 25.9 29.3 26.1 25.7 34.9 33.0 40.5 45.3 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ A naerobic Live Sterile <LOD <LOD <LOQ <LOQ 3.4 3.5 9.6 6.3 14.1 14.2 17.1 19.1 22.2 21.1 <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOQ LOD = limit o f detection (0.128 gg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 175 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 175- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 1 12E-108 Measured Concentrations of 8-2 FTOH(fig/kg) in Soils Treated with Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______ L ive Sterile Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD =limit o f detection (22.3 pg/kg) LOQ = limit of quantitation (100 pg/kg) i Monday, October 15, 2007 p. 176 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 176- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E -108 Measured Concentrations of 8-2 FTOH in Soils Treated with Soil Type Moitisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <L0D CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOQ CLOD CLOD cLOD CLOD CLOD CLOD <LOD CLOD <LOD CLOD <LOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD LOD = limit o f detection (22.3 pg/kg) LOQ = limit o f quantitation (100 pg/kg) A naerobic L ive Sterile CLOD CLOD <LOQ CLOQ CLOD CLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD Monday, October 15, 2007 p. 177 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -177- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTOH (pg/kg) in Soils Treated withQ Sam ple A erobic S o il Type_________ Interval__________ L ive Sterile Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Monthfi Month 9 Month 12 Month 15 Month 18 Month 24 CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD <LOD <LOD CLOD cLOQ CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD LOD = limit o f detection (22.3 gg/kg) LO.Q = limit o f quantitation (100 pg/kg) A naerobic L ive Sterile CLOD cLOD <LOD CLOD cLOD cLOD CLOD CLOD CLOD CLOD CLOQ CLOD CLOD CLOD CLOD CLOD CLOD cLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD ! Monday, October 15, 2007 I p. 178 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 178- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH (pg/kg) in Soils Treated with 1 Sam ple A erobic A naerobic S o il Type_________ Interva l_____________L ive Sterile_______L ive S terile Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD CLOD CLOD CLOD cLOQ cLOQ 174 125 148 138 cLOQ 140 100 <LOQ 131 165 173 121 cLOQ cLOQ CLOQ cLOQ CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOQ CLOD <LOD CLOD cLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD CLOQ CLOQ cLOQ cLOQ cLOQ cLOQ CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOQ CLOD cLOD <LOD cLOD CLOD LOD = limit o f detection (22.3 pg/kg) LOQ = limit o f quantitation (100 pg/kg) Monday, October IS, 2067 p. 179 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -179- Project No.: 112E-108 Wildttfe International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTOH (pg/L) in Headspaces Gases from Chambers Treated with 1 Sam ple A erobic A naerobic S o il Type_________ Interva l_____________L ive S terile_______ L ive S terile Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ O' O' oo LOQ = limit o f quantitation {12.5 pg/L of headspace) . Monday, October IS, 2007 p. 180 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 180- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E -108 Measured Concentrations of 8-2 FTOH (fig/L) in Headspaces Gases from Chambers Treated witta 1 Sam ple S o il Type_________ Interval_______ A erobic L ive Sterile Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <L0Q <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.5 pg/L o f headspace) A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <I,OQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 p. 181 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 181 - Projectno.: 112E-108 Wildlife International, Ltd._________________________ Project N um ber 112E-L08 Measured Concentrations of 8-2 FTOH (pg/L) in Headspaces Gases front Chambers Treated with Q 3 Sam ple S o il Type_________ In terva l A erobic L ive Sterile Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month + Month 6 Month 9 Month 12 Month IS Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.S pg/L o f headspace) A naerobic L iv e ...... Sterile^ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October JS, 2007 p. 182 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 182- Project No.: 112E-108 Wildlife International, Ltd._________________________ project N um ber 112E -108 Measured Concentrations of 8-2 FTOH (pg/L) in Headspaces Gases from Chambers Treated with(f J S o il Type Sam ple In te r v a l A erobic L ive Sterile InceD tisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 .<LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.5 pg/L o f headspace) A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October 15, 2007 p. 183 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -183- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 7-2 s FTOH (fig/kg) in Soils Treated with[_ ~] Sam ple S o il Type_________Interval_______ A erobtc L ive A naerobic S te r ile L iveSterile Ultiso) DayO, Month 2 Month 6 Montlt9 Month 12 Month IS . Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 133.0 166.1 <LOQ <LOQ <LOQ <LOQ 115.6 <LOQ <LOQ <LOQ <LOQ <LOQ' LOQ = limit ofquantitation (100 pg/kg) Thursday, November 08, 2007 i p. 184 Company Sanitized. Does not contain TSCA CBI. W ildlife International, L td. -184- ProjectNo.: 112E-108 Wildlife International, Ltd._______________ Project Number 112E-108 Measured Concentrations of 7-2 s FTOH (pg/kg) in Soils Treated withC ^ Sam ple A erobic S o il Type_________ Interval_____________L ive Sterile Mollisot DayO Month 2 Months Month? Month 12 Month 15 Month IS Month 24 <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ A naerobic L ive Sterile LOQ = limit o f quantitation (100 pg/kg) Thursday, Sovtmber OS, 2007 p. 185 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -185- projectno.: 112E-108 Wildlife International, Ltd, Project Num ber 112E-108 Measured Concentrations of 7-2 s FTOH (jig/kg) in Soils Treated with, "3 Sam ple S o il Type_________In terva l Alfisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ LOQ " limit of quantitation (100 pg/kg) A naerobic L ive Sterile Thursday, November 08, 2007 p. 186 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 186- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 7-2 s FTOH (jig/kg) in Soils Treated with j Sam ple A erobic A naerobic S o il Type________ Interval_____________L ive Sterile_______L ive Sterile InccD tisol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOQ <LOQ <LOQ <LOQ 278.3 278.3 192.8 464.2 193.0 118.9 173.2 182.2 <LOQ <LOQ O' O' oo Vv LOQ = limit o f quantitation (100 pg/kg) Thursday, November 08, 2007 p. 187 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 187 - projectNo.: 112E-108 Wildlife International, Ltd.__________________________Project N um ber U 2E -108 Measured Concentrations of 7-3 Acid (jig/kg) in Soils Treated with S o il Type Ultisol Sam ple In te r v a l Dy 0 Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 A erobic Live Sterile <LOQ <JLOQ 6.6 9.1 16.3 21.4 31.3 28.7 41.7 33.4 34.1 40.1 27.8 17.0 54.3 56.2 LOQ = limit o f quantitation (2.0 jig/kg) A naerobic L ive Sterile ' I Wednesday, March 12,2008 p. 188 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 188- Projectno.: 112E-108 Wildlife International, Ltd.________________ Project Number 112B-108 Measured Concentrations of 7-3 Acid (jig/kg) in Soils Treated with Q ^ Soil Type Mollisol Sam ple In te r v a l DayO Month 2 Months Month 9 Month 12 Month IS Month 18 Month 24 A erobic liv e Sterile <LOQ <LOQ <LOQ <LOQ 2.4 2.7 2.7 2.9 5.0 4.8 6.6 9.0 3.5 3.4 8.9 6.6 . LOQ - lim it o f quantitation (2.0 pg/kg) A naerobic L ive Sterile Wednesday, March 12,2009 p. 189 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -189- projectno.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 7-3 Acid (Mgj'kg) in Soils Treated with S o il Type Alflsol Sam ple In te r v a l Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 A erobic L ive Sterile <LOQ <LOQ <LOQ <LOQ 3.2 2.5 3.2 2.5 4.9 6.1 7.6 8.9 8.1 6.4 6.6 7.3 LOQ limit o f quantitation (2.0 ng/kg) A naerobic L ive Sterile \ Wednesday, March 12,2008 p. 190 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 190- Project No.: 112E-108 Wildlife International, Ltd, Project Num ber 112E-108 Soil Type Inceotisol Measured Concentrations of 7-3 Acid (fig/kg) in Soils Treated with(_ 3 Sam ple In te r v a l A erobic L ive Sterile A naerobic L ive Sterile Day 0 Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ LOQ 16.7 9.6 67.7 81.6 128.3 153.2 277.0 246.2 260.7 289.7 282.3 212.2 355.2 321.9 LOQ = limit o f quantitation (2.0 |ig/kg) Wednesday, March 12,2008 p. 191 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 191 - Project No.: 112E-108 Wildlife International, Ltd._____________Project Numbers 1 I2E-108, 109,110 Measured Concentrations of PFOA (jig/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Recoveries S o il Type_________ Interva l_______ A erobic A naerobic______A erobic______ A naerobic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 13 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ 9.5 7.8 . 8-6 9.1 9.2 9.2 10,4 10.3 12.0 11.4 10.6 10.4 9.2 10.0 7.4 6.8 95% 79% 96% 91% 92% 92% 204% 103% 120% 114% 106% 104% 92% 100% 74% 68% 9.5 9.2 10.5 11.3 9.2 9.8 9.3 9.0 11.4 10.8 11.4 11.3 11.2 11.5 95% 92% 105% 113% 92% 93% 93% 90% 114% 103% 114% 113% 112% 115% LOD = limit o f detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 Page 1 o f 4 p. 192 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 192 - Project No.: 112E-108 Wildlife International, Ltd. ProjectN um bersll2E-108,109,110 Measured Concentrations of PFOA (jig/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols S o il Type_________ Interval_______ A erobic A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 cLOD CLOD <LOD <LOD cLOD CLOD cLOQ cLOQ cLOD CLOD cLOQ CLOD cLOQ cLOQ cLOQ cLOD <LOD CLOD CLOD cLOD cLOQ LOD = limit o f detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) CLOD cLOD clod cLOQ cLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD F ortified Recoveries A erobic______ A naerobic 8.9 9.3 9,7 10.0 8.8 9.4 9.1 9.0 10.7 11.2 13.2 12.1 13.1 12.0 11.4 11.6 9.2 10.4 89% 93% 97% 100% 88% 94% 91% 90% 107% 112% 132% 121% 131% 120% 114% 116% 92% 104% 9.3 10.1 8.5 9.6 8.5 8.9 9.5 9.9 12.2 10.9 14.0 13.9 14.5 14.3 93% 101% 83% 96% 85% 89% 95% 99% 122% 109% 140% 139% 145% 143% Friday, October 12,2007 Page 2 of4 i i p. 193 Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. - 193- Project No.: 112E-108 Wildlife InternationalLtd,________________ Project Numbers 1126-108,109,110 Measured Concentrations of PFOA (pg/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l Untreated C ontrols A erobic A naerobic Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month LS Month 18 Month 24 CLOD CLOD cLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOQ cLOQ CLOD cLOD cLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD cLOD cLOD cLOD CLOD CLOD cLOD CLOD cLOD LOD = limit ofdetection (0.206 pg/kg) LOQ = limit ofquantitation (2.0 pg/kg) F o rtified R ecoveries A erobic A naerobic 9.2 8.3 8.9 10.0 9.9 9.4 10.0 10.1 11.0 10.7 10.8 11.5 11.0 11.1 11.5 12.5 9.2 9.3 92% 83% 89% 100% 99% 94% 100% 101% 110% 107% 108% 113% 110% 111% 115% 125% 92% 93% 9.2 8.2 8.7 9.3 9.6 10.0 10.6 10.4 10.4 10.7 11.1 10.9 10.5 11.2 92% 82% 87% 93% 96% 100% 106% 104% 104% 107% IL1% 109% 105% 112% Friday, October 12,202? Page 3 Of4 p. 194 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -194- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 1126-108,109,110 Measured Concentrations of PFOA (fig/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l U ntreated Controls A erobic A naerobic Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ CLOQ <LOQ LOD " limit o f detection (0.206 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 10.4 10.5 12.9 10.1 10.6 10.9 12.4 11.2 11.3 11.7 13.4 10.8 12.1 10.6 14.5 12.4 9.6 9.6 104% 105% 139% 101% 106% 109% 124% 112% 113% 117% 134% 108% 121% 106% 145% 124% 96% 96% 9.7 9.4 10.9 9.8 10.8 10.5 10.7 10.9 11.9 11.1 10.6 11.5 11.3 11.6 97% 94% 109% 98% 108% 105% 107% 109% 119% 111% 106% 115% 113% 116% Friday, October 33,3007 Pagg 4 f 4 p. 195 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -195- Project No.: 112E-108 ,Wildlife International Ltd________________ Project N um bers 112E-108,109,110 Measured Concentrations of 8-2 FTUA (jtg/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Recoveries S o il Type_________ Interva l_______ A erobic A naerobic______ A erobic______ A naerobic Ultisol ' DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOD cLOD <LOD <LOD CLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD 7.2 72% 6.5 65% 7.7 77% 7.9 79% 6.9 69% 6.9 69% 7.2 72% 7.2 72% 8.9 89% 8.0 80% 6.5 65% 7.3 73% 2.9 29% 5.4 54% 5.1 51% 4.0 40% 8.0 80% 7.4 74% 7.7 77% 8.7 87% 8.4 84% 8.2 82% 8.2 82% 7.9 79% 9.3 93% 7.6 76% 8.2 82% 8.0 80% 6.5 63% 7.0 70% LOD - limit of detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pgflkg) Friday, October 12,2007 Page 1 o f4 I \ p. 196 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 196- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 1126-108,109,110 Measured Concentrations of 8-2 FTUA (fig/kg) in Untreated and Fortified Soils Sam ple U ntreated Controls F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD CLOD CLOD CLOD cLOD cLOD CLOD <LOD cLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD cLOD cLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD CLOD 9.2 9.0 8.1 8.5 9.1 9.5 9.0 9.1 8.8 8.7 10.0 9.4 10.2 8.2 92% 90% 81% 85% 91% 95% 90% 91% 88% 87% 100% 94% 102% 82% 78% OO 9.0 90% 5.9 59% 6.3 65% 7.3 8.8 7.7 8.7 7.7 9.8 8.0 8.0 9.7 7.8 9.9 10.7 10,0 8.5 73% 88% 77% 87% 77% 98% 80% 80% 97% 78% 99% 107% 100% 85% L O D = limit of detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) . ' Friday, October 13, 3007 Page 3 of 4 p. 197 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 197 - Project No.: 112E-108 Wildlife International, Ltd. Project N um bers m E -1 0 8 ,1 0 9 ,110 Measured Concentrations of 8-2 FTUA (pg/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______A naerobic Alfisol , Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 CLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD cLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD cLOD CLOD 8.4 7.7 8.6 9.8 10.5 7.6 7.5 9.2 9.6 9.1 6.6 9.0 8.4 9.0 8.0 11.1 6.2 6.7 84% 77% 86% 99% 105% 76% 75% 92% 96% 91% 66% 90% 84% 90% 80% 111% 62% 67% 8.9 7.5 9.3 9.5 9.2 8.9 8.6 9.4 10.9 10.8 8.1 8.3 10,3 8.3 89% 75% 93% 95% 92% 89% 86% 94% 109% 108% 81% 83% 103% 83% LOD = limit o f detection (0.119 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 Pag* 3 c f 4 p. 198 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 198- ProjectNo.: 112E-108 Wildlife International, Ltd. P ro je ct N u m b ers 1i2E-io8.io9.no Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils Soil Type Incentisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month. 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 Untreated C ontrols A erobic A naerobic cLOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD CLOD LOD = limit of detection (0.119 pg/kg) LOQ = limit of quantitation (2.0 pg/kg) I F ortified Recoveries A erobic A n a ero b ic 11.0 9.6 10.8 8.3 8.8 9.7 9.4 7.9 8.0 8.9 10.1 8.1 7.0 6.8 7.4 6.4 5.3 5.2 110% 96% 108% 83% 88% 97% 94% 79% 80% 89% 101% 81% 70% 68% 74% 64% 33% 32% 7.9 79% 8.1 81% 8.3 83% 7.5 73% 8.5 85% 7.8 78% 7.7 77% 8.2 82% 7.6 76%. 8.8 88% 6.6 66% 7.0 70% 6.1 61% 7.1 71% Friday, October 3, 2007 Page 4 o f4 p. 199 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -199- ProjectNo.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (fig/kg) in Untreated and Fortified Soils Soil Type uteisoi Sam ple In te r v a l DayO Weekl Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 U ntreated C ontrols A erobic A naerobic <LOD <LOD CLOD CLOD cLOD CLOD CLOD CLOD <LOD CLOD cLOD cLOD CLOD cLOD cLOD cLOD cLOQ CLOQ CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD LOD limit o f detection (0.503 pgfltg) LOQ 3 limit o f quantitation (2.0 pg/kg) Values in parentheses are <LOQ and >--LOD F ort{ped Recoveries A erobic A naerobic 6.1 01% 5.8 58% 6.2 62% 6.9 69% 5.4 54% 5.5 55% 5.4 54% 5.3 53% 3.3 33% 2 .4 24% 3.4 34% 3.7 37% (1.4) (1 .4 ) 4.1 41% (1 .7 ) 6.7 67% 6.6 66% 7.0 70% 6.9 69% 6.1 61% 6.5 65% 5.3 53% 6.3 63% 7.2 72% 5.1 51% 4.8 48% 4.6 46% 3.5 35% 3.2 32% Friday, October 12, 2007 Page 1 o f4 ! p. 200 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 200- ProjectNo.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108.109,110 Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils S o il T ype Mollisol S a m p le In te rv a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 U n trea ted C o n tro ls A e ro b ic A n a e ro b ic <LOD <LOD 2.7 <LOD <LOD CLOD <LOQ cLOD cLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD LOD = limit o f detection (0.503 pg/kg) LOQ = limit o f quantitation (2.0 pg&g) Values in parentheses are <LOQ and >=LOD F o r tifie d R eco veries A e ro b ic A n a ero b ic 8.7 9.0 8.4 9.0 9.0 8.8 8.3 8.1 7.8 7.9 8.4 6.9 6,4 6.2 5.0 6.2 2.3 (1.71 8m 90* 84% 90% 90% B8* 83* 81* 78* 79% 84* 69% 64* 62* 30* 62* 23* 7.0 70* 8.7 87* 7.1 7 1* 7.6 76* 6.4 64* 8.3 83* 6.3 63* 7.0 70* 8.0 80* 7.1 71* 5.8 58* 6.5 65* 5.7 57* 5.2 52* Friday, October 12, 2007 Page 2 o f4 p. 201 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -201 - ProjectNo.: 112E-108 Wildlife International, Ltd. projectN um bersii2 E -io 8 , 1 0 9 ,1 1 0 Measured Concentrations of 8-2 FTA (pg/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F o rtified R ecoveries S o il Type_________ Interva l_______ A erobic A naerobic______A erobic_______A naerobic Atfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD cLOD cLOQ CLOD cLOD cLOD CLOD cLOD cLOD <LOD CLOD cLOD cLOD CLOD CLOD <LOQ CLOQ 7.8 7.9 7.8 8.4 10,6 8.7 6.8 8.4 7.5 7.0 4.2 6.7 6.0 6.6 5.1 7.7 4.0 3.7 78% 19% 11% 84% 106% 87% 68% 84% 75% 70% 42% 67% 60% 66% 51% 77% 40% 37% 8.8 88% 6.7 67% 8.5 85% 8.4 84% 8.4 84% 8.1 81% 7.7 77% 8.3 83% 8.8 88% 8.3 83% 6.7 67% 6.6 66% 7.3 73% 7.0 70% LOD " limit o f detection (0,503 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Values in parentheses are <LOQ and >=LOD Friday, October 13, 2007 Page 3 o f 4 p. 202 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 202- ProjectNo.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTA (fig/kg) in Untreated and Fortified Soils Soil Type InceDtlsol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Montil 18 Month 24 Untreated C ontrols A erobic A naerobic <LOD CLOD CLOD cLOD CLOD CLOD CLOQ cLOD CLOD cLOD cLOD cLOD cLOD CLOD CLOQ CLOQ cLOQ cLOD cLOD CLOD cLOQ CLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD LOD = limit o f detection (0.503 pg/kg) LOQ - limit o f quantitation (2.0 pg/kg) Values in parentheses are <LOQ and >=LOD F ortified Recoveries A erobic A n a ero b ic 10.7 9.6 9.8 7.6 7.0 8.4 7.1 6.3 5.5 5.8 5.3 4.5 4.3 3.7 3.4 3.4 2.1 n .8 i 107% 96% 98% 76% 70% 84% 71% 63% 53% 58% 53% 45% 43% 37% 34% 34% 21% 7.8 78% 7.2 72% 7.0 70% 6.7 67% 7,2 72% 7.6 76% 5.9 59% 6.6 66% 4.8 42% 6.3 63% 3.6 36% 3.4 34% 2.6 26% 3.5 35% Friday, October 12, 2007 Pag* 4 oj 4 p. 203 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -203- ProjectNo.: 112E-108 Wildlife InternationalLtd, Project Numbers 112E-108>109,110 Measured Concentrations of PFNA (fig/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified Recoveries S o il Type_________ In terva l_______ A erobic A naerobic______A erobic______ A naerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1$ Month IB Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <L0Q 9.4 7.8 9.7 9.8 9.5 9.8 10.4 10.1 11.4 11.4 10.3 10.6 7.0 10.3 7.9 6.7 94% 79% 97% 99% 95% 98% 104% 101% 114% 114% 103% 106% 70% 103% 79% 67% 10.0 9.9 9.9 10.7 9.7 10.3 10.3 9.6 11.2 11.0 11.6 11.7 10.8 11.6 100% 99% 99% 107% 97% 103% 103% 96% 112% 110% 116% 117% 108% 116% LOD " limit o f detection (0.124 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) Friday, October 12, 2007 Pago 1 o f4 p. 204 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -204- Project No,: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 11 2 E -1 0 8 ,1 0 9 ,1 10 Measured Concentrations of PFNA (pg/kg) in Untreated and Fortified Soils S o il Type MoUisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 Untreated Controls A erobic A naerobic <LOD CLOD cLOD CLOD CLOD CLOD CLOD cLOQ cLOD <LOD CLOD cLOD CLOD cLOD cLOQ cLOQ CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOQ CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD LOD = limit of detection (0.124 pg/kg) LOQ - limit of quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 9.4 9.6 9.7 9.7 9.5 9.9 10.2 8.6 10.7 10.6 12.2 11.9 12.0 11.7 10.0 11.2 8.1 8.3 94% 96% 97% 97% 95% 99% 102% 86% 107% 106% 122% 119% 120% 117% 100% 112% 81% 83% 9.6 10.1 8.9 9.8 9.6 9.9 9.3 9.8 10.7 10.1 11.6 12.8 12.2 11.9 96% 101% 89% 98% 96% 99% 93% 98% 107% 101% 116% 128% 122% 219% Friday, October 12, 2007 Page 2 o f4 p. 205 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -205- Project No.: 112E-108 Wildlife International, Ltd. Project N um bers 112E-108,109,110 Measured Concentrations of PFNA (fig/kg) in Untreated and Fortified Soils S o il Type Aliisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month. 2 Month 4 Month 6 Month 9 Month L2 Month IS Month 1$ Month 24 U ntreated C ontrols A erobic A naerobic <LOD <LOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOQ cLOQ CLOQ CLOQ cLOQ CLOQ CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOD cLOQ CLOQ LOD = limit o f detection (0.124 gg/kg) LOQ - limit o f quantitation (2.0 gg/kg) F ortified R ecoveries A erobic A naerobic 9.8 9.3 9.4 11.2 10.3 9.1 10.3 10.7 11.3 11.0 10.0 11.4 10.8 11.4 11.3 12.3 8.7 8 .6 98% 93% 94% U2% 103% 91% 103% 107% 113% no% 100% 114% 108% 114% m% 123% 87% 86% 9.7 8.1 9.4 10.1 9.8 10.1 10.3 10.0 11.0 11.0 10.7 10.7 11.5 1 0 .2 97% 81% 94% 101% 98% 101% 103% 100% 110% 110% 107% 107% 113% 102% Friday, October 12, 2007 Page 3 o f 4 p. 206 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 206- Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 1 12E -108,109,110 Measured Concentrations of PFNA (fig/kg) in Untreated and Fortified Soils Soil Type In ceD tiso l Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 U ntreated Controls A erobic A naerobic <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ. <LOQ <LOQ <LOQ LOD = limit o f detection (0.124 ng/kg) LOQ = limit o f quantitation (2.0 jig/kg) F ortified Recoveries A erobic A naerobic 12.4 11.6 13.0 10.6 10.7 10.7 12.5 10.7 11.2 11.9 14.0 10.5 10.4 10.0 13.0 10.9 8.9 8.8 124% 116% 130% 106% 107% 107% 125% 107% 112% 119% 140% 105% 104% 100% 130% 109% 89% 88% 10.2 10.0 10.8 9.8 10.3 10.2 10.1 10.7 11.1 11.1 11.0 10.8 10.1 10.8 102% 100% 108% 98% 103% 102% 101% 107% 111% 111% 110% 108% 101% 108% Friday, October 12, 2007 ii Page 4 o f4 p. 207 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -207- Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E -108,109,110 Measured Concentrations of PFDA (fig/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD - limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 9.3 7.4 8.9 8.6 8.8 8.9 9.4 9.3 10.9 10.9 9.6 10.0 5.2 9.5 8.4 7.0 93% 74% 89% 86% 88% 89% 94% 93% 109% 109% 96% 100% 52% 95% 84% 70% 9.2 9.1 9.0 9.7 9.4 9.2 9.3 8.0 10.4 10.2 11.0 10.9 9.6 10.4 92% 91% 90% 97% 94% 92% 93% 80% 104% 102% 110% 109% 96% 104% Friday, October 12, 2007 Page 1 o f 4 p. 208 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -208- Project No.: 112E-108 Wildtife International, Ltd. P roject N um bers 112E -108,109,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils S o il Type Mollisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 Untreated Controls A erobic A naerobic <LOQ <LOQ <LOD <LOD <LOD CLOD CLOQ CLOQ cLOD CLOD cLOD cLOD CLOD cLOD CLOQ cLOQ CLOD cLOD CLOD CLOD cLOQ CLOQ CLOQ <LOQ CLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD CLOD cLOD LOD =limit o f detection (0.114 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 10.0 10.0 9.8 10.4 9.7 10.1 9.8 8.1 9.9 9.7 11.7 10.9 12.1 11.2 9.2 10.2 7.6 8.0 100% 100% 98% 104% 97% 101% 98% 81% 99% 97% 117% 109% 121% 112% 92% 102% 76% 20% 9.2 9.9 8.2 9.5 9.2 9.3 9.0 9.0 10.4 9.2 11.4 12.1 11.2 10.9 92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109% Friday, October 12, 2007 Page 2 o/4 p. 209 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -209- Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 1 1 2 E - 1 0 8 ,10 9 ,110 Measured Concentrations of PFDA (pg/kg) in Untreated and Fortified Soils S o il Type Alfisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 U ntreated Controls A erobic A naerobic <LOD <LOD cLOD <LOD cLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOQ CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD CLOD cLOD cLOD CLOD cLOD cLOQ LOD = limit o f detection (0.114 pg/kg) LOQ " limit of quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 9.3 9.3 9.4 11.0 10.1 8.6 9.0 10.5 11.9 11.2 9.3 10.9 10.7 11.2 10.9 13.3 9.2 9.3 93% 93% 94% 110% 101% 86% 90% 103% 119% 112% 93% 109% 107% 112% 109% 133% 92% 93% 9.2 92% 7.4 74% 8.7 87% 9.3 93% 9.0 90% 9.5 93% 9.7 97% 9.6 96% 11.3 113% 11.4 114% 9.0 90% 9.6 96% . 11-6 116% 8.6 86% Friday, October 22, 2007 Page 3 o f4 p. 210 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 210- Project No.: 112E-108 Wildlife International, Ltd. ProjectN um bersll2E-108,109,110 Measured Concentrations of PFDA (fig/kg) in Untreated and Fortified Soils Soil Type InceDtisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 U ntreated Controls A erobic A naerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit o f detection (0.114 jig/kg) LOQ = limit o f quantitation (2,0 pglkg) F ortified Recoveries A erobic A naerobic 13.9 12.4 14.6 11.6 11.3 11.6 12.1 11.5 12.0 13.3 14.1 10.8 10.7 9.2 12.8 10.9 9.5 9.2 139% 124% 146% 116% 113% 116% 121% 116% 120% 133% 141% 108% 107% 92% 128% 109% 95% 92% 9.9 9.7 10.7 9.6 10.8 10.5 10.1 10.7 11.8 12.2 10.8 11.6 9.6 10.6 99% 97% 107% 96% 108% 103% 101% 107% 118% 122% 108% 116% 96% 106% Friday, October 12, 2001 Page 4 o f4 p. 211 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -211 - Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 1 1 2 E -1 0 8 >109,110 Measured Concentrations of PFUA (pg/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F o rtified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic______ A naerobic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOD <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOD <L0Q <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q 8.5 6.9 8.2 8.2 7.6 7.8 8.6 7.4 10.2 10.1 9.2 9.3 4.1 8.4 7.2 6.2 85% 69% 91% 82% 7614 78% 86% 74% 102% 101% 92% 93% 41% 84% 72% 62% OO 00 8.5 83% 88% 8.5 83% 8.4 84% 8.8 88% 8.6 86% 9.1 91% 7.0 70% 9.8 98% 9.5 95% 9.4 94% 9.5 95% 8.8 88% 9.9 99% LOD " limit o f detection (0.128 pg/kg) LOQ = limit of quantitation (2.0 pg/kg) Friday, October 12, 2007 p. 212 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 212- Project No.: 112E-108 Wildlife International, Ltd. project Numbers i i2E-io8,io9,no Measured Concentrations of PFUA (ng/kg) in Untreated and Fortified Soils Soil Type Mollisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month Month 9 Month 12 Month IS Month 18 Month 24 Untreated C ontrols A erobic A naerobic <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD COD <LOD <LOD COD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ COQ <LOD <LOD <LOD <LOD COD <LOD <LOD <LOD <LOD <LOD COD LOD = limit o f detection (0.128 pg/lcg) LOQ =limit o f quantitation (2.0 pg/kg) F ortified Recoveries A erobic A naerobic 9.5 9.6 8.7 9.3 9.0 9.2 8.7 6.9 8.4 8.4 10.1 10.1 9.5 8.7 7.4 8.8 6.1 6.3 95% 96% 87% 93% 90% 92% 87% 69% 84% 84% 101% 101% 93% 87% 74% 88% 61% 63% 8.2 82% 8.6 86% 6.7 67% 7.6 76% 6.3 63% 7.1 71% 6.3 63% 8.0 80% 8.5 85% 7.5 75% 9.6 96% 9.4 94% 9.8 98% 7.9 79% Friday, October 12, 2007 p. 213 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -213- Project No.: 112E-108 Wildlife International, Ltd. Project N um bers 112E -108, 109,110 Measured Concentrations of PFUA (jig/kg) in Untreated and Fortified Soils S o il Type Alfisol Sam ple In te r v a l DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 U ntreated C ontrols A erobic A naerobic <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOQ cLOQ <LOD <LOD <LOD cLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD <LOQ cLOQ LOD = limit o f detection (0.128 pg/kg) LOQ = limit of quantitation (2.0 pg/kg) F ortified R ecoveries A erobic A naerobic 9.2 8.7 9.2 10.9 9.4 8.0 7.7 9.4 10.3 9.7 7.5 8.9 8.9 9.3 8.6 12.2 8.7 8.8 92% 87% 92% 109* 94* 80* 77% 94% 103% 97% 73% 89% 89% 93% 86% 122% 87% 88% 8.3 83% 6.1 61% 8.3 83% 8.6 86% 7.9 79% 7.9 79% 7.0 70% 7.6 76% 8.8 88% 9.9 99% 7.4 74% 7.2 72% 9.5 95% 6.7 67% Friday, October 12, 2007 p. 214 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -214- Project No.: 112E-108 Wildlife International, Ltd. P ro je c tN u m b e rs ll2 E -1 0 8 ,109,110 Measured Concentrations of PFUA (|xg/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l Untreated C ontrols A erobic A naerobic Incentisol DayO W eek 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <lod <LOD <LOQ <LOQ LOD = limit o f detection (0.128 pg/kg) LOQ = limit o f quantitation (2.0 pg/kg) F ortified Recoveries A erobic A n a ero b ic 12.0 in 12.5 10.1 9.2 10.3 9.8 8.9 10.4 11.7 12.2 8.8 8.2 8.3 9.9 . 8.2 7.4 6.9 120% 112% 125% 101% 92% 103% 98% 89% 104% 117% 122% 88% 82% 83% 99% 82% 74% 49% 7.9 7.4 7.9 7.4 7.4 8.7 8.0 8.5 8.7 10.3 6.9 7.3 7.0 8.5 79% 74% 79% 74% 74% 87% 80% 83% 87% 103% 69% 73% 70% 85% Friday, October 1>, 2007 p. 215 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -215- Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E -108,109,110 Measured Concentrations of 8-2 FTOH (pg/kg) in Untreated and Fortified Soils Soil Type Ultisol Sam ple In te r v a l DayO W eekt Week 2 Week 4 Month 2 Month 4 Month 6 Month? Month 12 Month 13 Month IS Month 24 Untreated C ontrols A erobic A naerobic F ortified Recoveries A erobic A naerobic <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD <LOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD 289 283 244 247 210 208 165 147 102 / 98 ) ( 45 ) ( 53 ) ( 41 ) CLOD CLOD CLOD CLOD CLOD 113% 113% 97% 99% 84% 83% 66% 59% 41% 39% 18% 21% 16% 0% 0% 0% 0% 0% 291 116% 288 115% 220 88% 258 103% 249 181 179 155 165 117 ( 87 1 ( 54) ( 68 ) 99% 73% 71% 62% 66% 47% 35% 22% 27% LOD = limit o f detection (22.3 pg/kg) LOQ = limit o f quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD Monday, October IS, 2007 p. 216 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 216- Project No.: 112E-108 Wildtife International, Ltd. P ro ject N um bers 112E -108,109,110 Measured Concentrations of 8-2 FTOH (fig/kg) in Untreated and Fortified Soils Soil Type Mollisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 U ntreated C ontrols A erobic A naerobic F ortified Recoveries A erobic A naerobic <LOD <LOD <LOD <L0D CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD 299 12094 21 104% 213 85% 217 87% 194 78% 1 1 2 45% 119 48% ( 51 ) 21% ( 63 ) 25% CLOD 0% < 32 ) 13% CLOD 0% CLOD 0% CLOD 0% CLOD 0% CLOD 0% CLOD 0% 266 104% 279 112% 244 98% 273 109% 257 , 103% 243 97% 191 77% 192 77% ( 9 9 ) 40% 162 45% i 7 0 ) 28% ( 46 ) 18% ( 36 ) 14% CLOD 0% LOD = limit o f detection (22.3 pg/kg) LOQ = limit o f quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD < Monday, October IS, 2)07 p. 217 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -217- Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (jig/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______A naerobic Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 M onths Month 9 Month 12 Month 1$ Month 18 Month 24 <LOD CLOD CLOD CLOD CLOD CLOD CLOD ' CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD cLOD cLOD CLOD 307 123% 245 98% 210 84% 143 57% 156 62% 142 57% 117 47% 112 45% ( 75 ) 30% ( 79 ) 32% < 54 ) 22% ( 51 ) 21% CLOD 0% ( 29 ) 12% CLOD CLOD 0% 0% cLOD 0% cLOD 0% 267 107% 254 102% 215 86% 203 81% 205 82% 161 64% 127 51% 143 57% ( 81 1 33% ( 62 ) 23% ( 5 0 ) 20% ( 3 7 ) 15% CLOD CLOD 0% 0% LOD " limit o f detection (22.3 pg/kg) LOQ = limit o f quantitation (100 pg/kg) Values in parentheses are <LOQ and >=LOD Monday. October IS, 2007 p. 218 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -218- Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 1 12E - 108, 1 0 9 ,1 10 Measured Concentrations of 8-2 FTOH (fig/kg) in Untreated and Fortified Soils S o il Type Incentisol Sam ple In te r v a l Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 Untreated C ontrols A erobic A naerobic F ortified R ecoveries A erobic A naerobic <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD 285 114% 262 105% 267 10W 285 114* 245 99% 234 94% 218 97% 284 114* 201 91% 200 80% 157 63% 186 74% ( 86 1 34% ( 85 ) 34% ( 55 1 22% 115 46% CLOD 0% CLOD 0% 287 115% 285 114% 258 103% 260 104% 237 95% 235 94% 230 92% 219 87% 134 54% 165 66% 106 42% 106 42% ( 42 1 17% ( 37 1 13% LOD = limit o f detection (22.3 pg/kg) LOQ - limit o f quantitation (100 pg/ltg) Values in parentheses are <LOQ and >=L0D Monday, October 15, 2007 p. 219 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -219- Project No.: 112E-108 Wildlife International, Ltd. Project N um bers m E -1 0 8 ,1 0 9 ,1 1 0 Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Soils S o il Type_________ Interval_______ A erobic A naerobic______A erobic A naerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 M onths Month 9 Month 12 Month 1$ Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.5 pg/L o f headspace) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 1 o f4 p. 220 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -220- Project No.: 112E-108 Wildlife International, Ltd. Project Num bers 112B-108,109,110 Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils iSam ple Untreated C ontrols F ortified Soils S o il Type_________ Interval_______ A erobic A naerobic______ A erobic A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ " limit o f quantitation (12.5 pg/L o f headspace) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 2 of4 p. 221 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -221 - Project No.: 112E-108 Wildlife International, Ltd. Project N um bers m E -1 0 8 ,1 0 9 ,1 1 0 Measured Concentrations of 8-2 FTOH (fig/L) in Headspace Gases from Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Soils S o il Type_________ Interval_______ A erobic A naerobic______A erobic A naerobic Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.S pg/L o f headspace) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Fag* 3 o f 4 p. 222 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -222- Project No.: 112E-108 'Wildlife International, Ltd. P roject N um bers 112E -108,109,110 Measured Concentrations of 8-2 FTOH (pg/L) in Headspace Gases from Untreated and Fortified Soils Soil Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Inceotisol Day 0 Week t Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (12.5 pg/L o f headspace) F ortified Soils A erobic A naerobic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 4 of4 p. 223 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -223 Appendix II Measured Concentration of Selected Analytes (pmol/kg Soil Dry Weight) p. 224 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -224- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of PFOA (ftmol/kg) in Soils Treated with Soil Type Sam ple In te r v a l A erobic L ive Sterile Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.043 0.045 0.077 0.088 0.140 0.148 0.295 0.362 0.651 0.903 0.909 1.065 1.176 1.349 0.888 0.831 0.821 0.876 0.992 0.794 0.884 0.795 LOD - limit o f detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0,0048 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.006 <LOQ 0.005 <LOQ 0.012 <LOQ 0.019 <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ 0.053 0.055 0.065 0.058 0.124 0.097 0.169 0.177 0.277 0.322 0.359 0.253 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.006 <LOQ 0.007 <LOQ Thursday, November 08, 2007 p. 225 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -225 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um ber 112E-108 Measured Concentrations of PFOA (fimol/kg) in Soils Treated with "3 Sam ple A erobic A naerobic S o il Type_________ Interval____________ L ive Sterile_______ L ive Sterile Moilisol Day 0 Week 1 Week 2 Week 4 ' Month 2 Month 4 Months Month 9 Month 12 Month 15 Month IS Month 24 <LOQ <LOQ 0.037 0.036 0.043 0.044 0.052 0.049 0.077 0.073 0.111 0.142 0.180 0.180 0.223 0.167 0.251 0.227 0.238 0.599 0.307 0.287 0.554 0.501 . LOD " limit of detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0,0048 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.007 <LOQ 0.008 0.006 0.008 0.008 0.007 0.008 <LOQ <LOQ 0.035 0.036 0.045 0.044 0.049 0.054 0.103 0.104 0.189 0.149 0.284 0.220 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.006 <LOQ 0.009 0.006 Tkursday, November OS, 007 I p. 226 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -2 2 6 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um ber 112E-108 Measured Concentrations of PFOA frunol/kg) in Soils Treated with C J S o il Type Sam ple A erobic Interval_____________L ive Sterile O' O' oo Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 M onth 24 0.020 0.020 0.027 0.026 0.036 0.038 0.056 0.060 0.077 0.080 0.141 0.127 0,236 0.226 0.158 0.203 0.321 0.250 0.469 0.376 0.475 0.460 LOD = limit o f detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0.0048 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ 0.008 <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ 0.023 0.026 0.034 0.037 0.048 0.048 0.043 0.075 0.217 0.188 0.240 0.202 <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.005 <LOQ Friday, October 12,2007 p. 227 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -227- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFOA Qimol/kg) in Soils Treated withQ, 0 S o il Type Sam ple In te r v a l A erobic liv e Sterile Incentisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ 0.046 0.050 0.069 0.070 0.135 0.132 0.295 0.236 0.460 0.457 0.639 0.605 0.697 0.820 0.888 0.845 0.972 0.930 1.020 1.078 1.282 1.338 LOD limit of detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0.0048 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ 0.007 <LOQ 0.011 <LOQ 0.013 0.007 0.015 0.007 0.010 0.006 A naerobic L ive Sterile <LOQ <LOQ 0.050 0.043 0.064 0.065 0.182 0.116 0.333 0.349 0.687 0.731 0.790 0.838 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.012 <LOQ 0.018 <LOQ F riday, O ctober 1 2 , 2 0 0 7 p. 228 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -228 - Project No,: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTUA tumol/kg) in Soils Treated with . "3 S o il Type Sam ple In te r v a l A erobic L ive Sterile Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 <LOD <LOQ <LOQ <LOQ 0.005 0.006 0.009 0.007 0.014 0.015 0.027 0.028 0.026 0.042 0.067 0.043 0.013 0.017 0.025 0.029 0.034 0.014 0.034 0.071 LOD = limit o f detection (0.0003 pmol/kg) LOQ - limit o f quantitation (0.0044 (tmol/kg) CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD cLOD CLOD A naerobic L ive Sterile CLOD CLOD <LOD CLOD <LOQ cLOQ <LOQ CLOQ cLOQ CLOQ cLOQ CLOQ cLOQ cLOQ CLOD CLOD CLOD CLOD <LOD cLOD cLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD j Friday, October 12, 2007 p. 229 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -229- Project No.: 112E-108 Wildtife InternationalLtd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTUA (jimol/kg) in Soils Treated with|_ J Sam ple A erobic A naerobic S o il T fp e_________ Interva l_____________L ive Sterile_______ L ive Sterile Mollisol ' DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 . Month 12 Month IS Month L8 Month 24 <LOQ <LOQ <LOD <LOD cLOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ CLOQ <LOQ <LOD <LOD <LOQ <LOQ 0.007 <LOQ LOD = limit o f detection (0.0003 pmol/kg) LOQ 3 limit o f quantitation (0.0044 pmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD cLOD cLOD CLOD CLOD cLOD CLOD CLOQ cLOQ CLOD CLOD cLOQ CLOQ <LOQ cLOQ cLOD CLOD CLOD cLOQ cLOQ cLOQ cLOQ cLOQ cLOQ <LOQ cLOD CLOD cLOQ CLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD CLOQ cLOD CLOD Friday, October 12, 2007 p. 230 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -230- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of 8-2 FTUA (pmol/kg) in Soils Treated with Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive Sterile Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month $ Month 12 Month 15 Month IS Month 24 <LOD CLOD CLOD CLOD CLOD CLOD CLOQ cLOQ CLOQ CLOD CLOD CLOD cLOQ cLOQ CLOQ cLOQ cLOQ cLOQ cLOQ cLOQ cLOQ cLOQ cLOQ cLOQ LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit of quantitation (0.0044 pmol/kg) CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOQ cLOQ CLOD CLOD CLOD CLOD CLOD cLOD cLOQ cLOQ CLOD CLOQ 0.008 0.005 0.005 cLOQ CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOQ cLOQ Frida,, October 12, 2007 p. 231 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -231 - Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTUA (pmol/kg) in Soils Treated with 3 Soil Type Sam ple In te r v a l A erobic L ive Sterile Incentisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD cLOQ <LOQ 0.013 0.011 0.055 0.027 0.170 0.194 0.358 0.350 0.419 0.352 0.457 0.452 0.492 0.550 0.559 0.530 0.542 0.522 LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0044 jimol/kg) -CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD 0.006 <LOQ 0.005 <LOQ cLOQ <LOQ A naerobic L ive Sterile cLOD <LOD <LOD CLOD CLOD CLOQ 0.017 CLOQ 0.012 0.012 0.028 0.028 0.034 0.022 CLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOQ CLOQ \ III i Friday, October 11,1007 p. 232 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -232- ProjectNo.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTA ftimol/kg) in Soils Treated with{_ 3 Sam ple A erobic A naerobic S o il ty p e _________ Interval_____________L ive Sterile_______L ive S terile Ultisoi Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 <LOD <LOD <LOQ <LOQ 0.008 0.008 0.013 0.010 0.018 0.018 0.020 0.017 0.021 0.040 0.041 0.025 0.006 0.009 0.009 0.008 0.014 0.006 0.010 0.011 LOD = limit o f detection (0.0011 pmol/kg) LOQ - limit o f quantitation (0.0042 pmol/kg) <LOD CLOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOQ CLOD cLOD CLOD CLOD 0.004 CLOD CLOD <LOD CLOD CLOD CLOQ CLOD CLOD cLOD cLOD CLOD CLOQ CLOQ CLOD cLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD Friday, October l , 07 p. 233 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -233 - ProjectNo.: 112E-108 Wildlife International, Ltd. P roject N um ber 112 E -108 Measured Concentrations of 8-2 FTA Ounol/kg) in Soils Treated withQ" J S o il Type Sam ple In te r v a l A erobic L ive Sterile Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD <LOQ 0.005 CLOD CLOD <LOD CLOD cLOQ cLOQ LOD " limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 (unol/kg) CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD A naerobic L ive Sterile CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD Friday, October 12, 2097 p. 234 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -234- ProjectNo.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTA (pmol/kg) in Soils Treated with 1 Sam ple A erobic A naerobic S o il Type_________ Interval____________ L ive Sterile_______L ive S ten le Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD CLOD CLOD CLOD CLOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOQ CLOQ LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg) CLOD CLOD cLOD <LOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOQ cLOQ CLOD CLOD CLOQ cLOQ cLOQ cLOQ CLOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD cLOQ Friday, October 12, 2007 p. 235 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -235 - ProjectNo.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTA (pmol/kg) in Soils Treated with Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive S terile Incentlsol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 - Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOQ <LOQ 0.011 0.010 0.038 0.027 0.154 0.158 0.341 0.302 0.343 0.265 0.279 0.294 0.404 0.430 0.447 0.474 0.423 0.464 LOD = limit o f detection (0.0011 junol/kg) LOQ =limit o f quantitation (0.0042 pmol/kg) <LOD <LOD <LOD <LOD <LOD cLOD <LOD <LOD <LOD <LOD <LOQ <LOD 0.006 <LOQ 0.006 <LOQ <LOQ <LOQ <LOD CLOD cLOQ CLOD CLOD CLOD 0.014 cLOQ 0.005, 0.005 0.013 0.012 0.018 0.011 cLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD cLOQ Friday, October 12, 2007 p. 236 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -236- Project No.: 112E-108 Wildlife International, Ltd. P roject N um ber 1 12E-108 Measured Concentrations of PFNA (pmol/kg) in Soils Treated witlj^ 0 Sam ple A erobic A naerobic S o li Type_________ Interval_____________L ive Sterile_______L ive Sterile TJltisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.015 0.016 0.025 0.026 0.038 0.036 0.052 0.046 0.060 0.065 0.061 0.066 0.072 0.065 0.018 0.034 0.051 0.057 0.064 0.053 0.067 0.064 LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmoVkg) <LOQ CLOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.014 0.015 0.020 0.020 0.034 0.029 0.033 0.046 0.050 0.048 0.065 0.055 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October 13, 3007 p. 237 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -237 - Project No.: 112E-108 Wildlife International, Ltd. P ro ject N um ber 112E-108 Measured Concentrations of PFNA (junol/kg) in Soils Treated with J Soil Type Sam ple In te r v a l A erobic L ive Sterile M ollis ol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month Month 18 Month 24 cLOQ <LOQ 0.013 0.013 0.012 0.018 0.023 0.026 0.039 0.037 0.048 0.048 0.046 0.043 0.037 0.024 0.042 0.040 0.031 0.056 0.045 0.037 0.055 0.075 LOD - limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) <LOD CLOD cLOQ CLOQ CLOD CLOD CLOQ CLOQ CLOQ cLOQ CLOQ cLOQ cLOQ cLOQ CLOQ cLOQ cLOQ CLOQ A naerobic L ive S ta ile CLOQ cLOQ 0.009 0.009 0.013 0.010 0.009 0.013 0.014 0.018 0.026 0.020 0.034 0.026 CLOD CLOD CLOQ CLOQ cLOQ <LOD cLOQ CLOQ CLOQ CLOQ CLOQ cLOQ CLOQ CLOQ Friday, October 12, 2007 p. 238 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -238- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of PFNA (iimol/kg) in Soils Treated with J Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive Sterile O 'O ' OO Alflsol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 0.007 0.009 0.010 0.008 0.014 0.017 0.022 0.035 0.023 0.029 0.019 0.034 0.038 0.050 0.029 0.038 0.050 0.033 0.034 0.038 0.054 0.047 LOD = limit o f detection (0.0003 junol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) CLOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ CLOD cLOQ cLOQ cLOQ <LOD <LOQ <LOQ cLOQ <LOQ cLOQ 0.005 0.006 0.007 0.010 0.010 0.008 0.014 0.019 0.020 0.024 0.017 cLOQ cLOQ cLOQ CLOD CLOQ CLOD CLOQ cLOQ cLOQ cLOQ cLOQ CLOQ CLOQ cLOQ Friday, October 12, 2007 p. 239 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -239- Project No.: 112E-108 Wilcllife International, Ltd. Project N um ber 1 12E-108 Measured Concentrations of PFNA (junol/kg) in Soils Treated with ^ Soil Type Sam ple In te r v a l A erobic L ive Sterile Incentisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.044 0.054 0.062 0.072 0.079 0.076 0.134 0.138 0.128 0.133 0.146 0.172 0.172 0.171 0.154 0.152 0.159 0.160 0.171 0.174 0.208 0.224 LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ 0.021 0.016 0.028 0.029 0.075 0.047 0.053 0.057 0.062 0.077 0.073 0.078 <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October l i , 1007 p. 240 Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. -240- Project No.: 112E-108 Wildlife International, Ltd. P ro ject N um ber 1 12E-108 Measured Concentrations of PFDA (pmolTkg) in Soils Treated with ) Sam ple A erobic A naerobic S o il Type_________ Interva l_____________L ive Sterile_______L ive Sterile Ultisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ 0.009 0.009 0.015 0.016 0.021 0.023 0.035 0.036 0.052 0.055 0.059 0.069 0.074 0.076 0.018 0.041 0.065 0.066 0.093 0.071 0.099 0.086 LOD = limit o f detection (0.0002 pmol/kg) LOQ ~ limit of quantitation (0.0039 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.009 0.010 0.013 0.014 0.018 0.016 0.026 0.029 0.049 0.055 0.043 0.038 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Thursday, November OS, 2007 I p. 241 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -241 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um ber 1 12E-108 Measured Concentrations of PFDA (fimol/kg) in Soils Treated with S o il Type Sam ple In te r v a l A erobic L ive Sterile Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOQ <LOQ 0.007 0.007 0.009 0.010 0.012 0.012 0.019 0.020 0.028 0.032 0.034 0.034 0.032 0.024 0.038 0.036 0.028 0.054 0.043 0.042 0.088 0.094 LOD limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg) <LOQ <LOQ <EOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ 0.007 0.007 0.010 0.009 0.013 0.013 0.021 0.021 0.034 0.028 0.041 0.034 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Thursday, November 08, 2007 p. 242 C o m p an y S an itized . D o es no t contain TSC A CBI. W ildlife International, Ltd. -242- Project No.: 112E-108 Wildlife International, Ltd. P roject'N um ber 1 12E-108 Measured Concentrations of PFDA (punol/kg) in Soils Treated with^ ^ Sam ple A erobic S o li Type_________ Interval_____________L ive Sterile AIGsol . DayO Week 1 . Week 2 Week 4 Month 2 Month 4 Month Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ 0.004 0.006 0.006 0.009 0.009 0.016 0.018 0.016 0.019 0.018 0.024 0.033 0.038 0.025 0.031 0.051 0.030 0.045 0.040 0.073 0.071 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ LOD = limit of detection (0.0002 pmol/kg) LOQ - limit o f quantitation (0.0039 pmol/kg) A naerobic L ive Sterile <LOQ <LOQ <LOQ 0.005 0.006 0.007 0.011 0.011 0.009 0.017 0.025 0.026 0.028 0.024 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October 12, 2007 p. 243 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -243 - Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFDA (jimol/kg) in Soils Treated with J Soil Type Sam ple In te r v a l A erobic liv e Sterile InceDtiso! DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.009 0.010 0.016 0.016 0.030 0.030 0.067 0.058 0.085 0.098 0.102 0.134 0.130 0.111 0.121 0.136 0.122 0.128 0.158 0.151 0.169 0.174 LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 <LOQ 0.005 <LOQ 0.006 0.006 0.006 0.005 0.004 0.004 A naerobic L ive Sterile <LOQ <LOQ 0.010 0,009 0.015 0.015 0.041 0.022 0.042 0.045 0.065 0.076 0.091 0.080 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 <LOQ Friday, October 12, 2007 p. 244 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -244- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFIJA Qunol/kg) in Soils Treated with S o il Type Sam ple In te r v a l A erobic L ive Sterile Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 0.005 0.006 0.009 0.008 0.015 0.014 0.023 0.027 0.028 0.029 0.033 0.030 0.006 0.019 0.027 0.028 0.034 0.029 0.040 0.040 LOD " limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ 0.005 0.005 0.007 0.007 0.012 0.015 0.027 0.023 0.032 0.031 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Friday, October 12, 2007 p. 245 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -245- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA ol/kg) in Soils Treated wittC Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive S terile Mollisol DayO Week 1 Week 2 Week 4 Month2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LQQ <LOQ 0.004 0.005 0.005 0.011 0.010 0.018 0.019 0.017 0.017 0.015 0.008 0.016 0.015 0.011 0.020 0.018 0.016 0.026 0.038 LOD - limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.004 0.004 0.006 0.011 0.008 0.012 0.010 <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD Friday, October 12, 2007 p. 246 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -246- Project No.: 112E-108 Wildlife International, Ltd. Project Number 112E-108 Measured Concentrations of PFUA (pmol/kg) in Soils Treated with(_ .] Sam ple A erobic A naerobic S o il Type_________ Interval____________ L ive Sterile_______ L ive Sterile Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 . Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOQ <LOQ <LOQ <LOQ 0.004 0.004 0.008 0.011 0.007 0.011 0.006 0.011 0.013 0.020 0.014 0.016 0.024 0.010 0.012 0.013 0.025 0.018 LOD - limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOQ <LOQ <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.005 0.005 0.006 0.008 0.006 <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOD <LOD <LOQ <LOQ Friday, October 13, 2007 p. 247 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -247- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 1 12E-108 Measured Concentrations of PFUA (pmol/kg) in Soils Treated withjT 1 S o il Type Sam ple In te r v a l A erobic liv e Sterile A naerobic L ive Sterile InecDtlsol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 1$ Month 24 <LOQ <LOQ CLOQ 0.004 0.007 0.008 0.013 0.011 0.034 0.035 0.033 0.033 0.037 0.050 0.052 0.050 0.046 0.052 0.046 0.045 0.062 0.058 0.072 0.080 LOD =>limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ CLOQ <LOQ <LOQ <LOQ <LOQ <LOQ cLOQ <LOQ <LOD <LOD cLOQ cLOQ 0.006 0.006 0.017 0.011 0.025 0.025 0.030 0.034 0.039 0.037 CLOD CLOD CLOD CLOD CLOD <LOQ cLOQ CLOD CLOQ cLOQ cLOQ CLOD cLOQ cLOQ Friday, October 12, 2007 p. 248 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -248 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um ber 112 E -108 Measured Concentrations of 8-2 FTOH (fimoL/kg) in Soils Treated witii 3 Soil Type Sam ple In te r v a l A erobic L ive Sterile Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <L0Q <L0Q <LOD <LOD <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ cLOD <L0Q cLOQ <LOQ <LOQ <LOD <L0D LOD = limit o f detection (01048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg) cLOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD <LOD CLOD CLOD CLOD A naerobic L ive Sterile CLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD <LOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD Monday, October IS, 2007 p. 249 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -249- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH Qunol/kg) in Soils Treated with 3 Soil Typo Sam ple In te r v a l A erobic L ive Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOQ CLOD CLOD LOD = limit o f detection (0.048 pmol/kg) LOQ " limit o f quantitation (0,216 pmol/kg) CLOD cLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD <LOD CLOD CLOD CLOD A naerobic L ive Sterile CLOD cLOD cLOQ CLOQ cLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD CLOD CLOD Monday, October 15, 2007 p. 250 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -250- Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 8-2 FTOH (pmol/lcg) In Soils Treated withQ D Soil Type Sam ple In te r v a l A erobic L ive Sterile A naerobic L ive Sterile Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD cLOD cLOD cLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOQ cLOD LOD = limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0,216 pmol/kg) <LOD CLOD <LOD cLOD <LOD CLOD CLOD cLOD CLOD CLOD <LOD CLOD cLOD CLOD cLOD <LOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD <LOD cLOD CLOD cLOD cLOQ CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD Monday, October IS, 2007 p. 251 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -251 - Project No.: 112E-108 Wildlife International, L td Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH (pmol/kg) in Soils Treated withf. 1 Soil Type Sam ple In te r v a l A erobic L ive Sterile InceDtisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 ' Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD CLOD CLOD CLOD CLOQ CLOQ 0.374 0.269 0.319 0.297 cLOQ 0.302 0.216 CLOQ 0.282 0.356 0.374 0.261 cLOQ cLOQ cLOQ cLOQ LO D = limit o f detection (0.048 pmol/kg) LO Q *limit o f quantitation (0.216 pmol/kg) CLOD CLOD CLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD cLOQ CLOD CLOD CLOD cLOD CLOD CLOD cLOD A naerobic L ive Sterile CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD CLOQ CLOQ cLOQ cLOQ CLOQ cLOQ cLOD CLOD cLOD CLOD cLOD cLOD CLOD CLOD cLOQ CLOD cLOD CLOD CLOD CLOD 1 ! Monday, October IS, 2007 p. 252 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -252- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated with 0 Soil Type Sam ple In te r v a l A erobic L ive Sterile A naerobic L ive Sterile Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L of headspace) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 p. 253 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -253 - Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations,of 8-2 FTOH (p.mol/L) in Headspaces Gases from Chambers Treated witbC 3 Soil Type Sam ple In te r v a l A erobic L ive Sterile Mollisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month $ Month? Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ =>limit o f quantitation (0.027 pmol/L o f headspace) A naerobic L ive Sterile <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <1,0 Q <LOQ <LOQ <LOQ Monday, October IS , 2007 p. 254 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -254- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspaces Gases from Chambers Treated with 1 S o il Type Sam ple In te r v a l A erobic L ive Sterile A naerobic liv e Sterile Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 M onths Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L o f headspace) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <L0Q <LOQ <LOQ <LOQ Monday, October 15, 2007 p. 255 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -255 - Project No.: 112E-108 ,WiIdlife International Ltd._________________________Project N um ber 112E-108 Measured Concentrations of 8-2 FTOH (ji.mol/L) in Headspaces Gases from Chambers Treated with[_ 3 Soil Type Sam ple In te r v a l A erobic L ive Sterile Incentisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1$ Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L o f headspace) A naerobic L ive Sterile <LOQ <LOQ <;LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 p. 256 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -256- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 7-2 s FTOH QunoL/kg) in Soils Treated with(_ Soil Type Sam ple In te r v a l A erobic L ive Sterile Uitisol DayO Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ 0.321 0.401 <LOQ <LOQ <LOQ <LOQ 0.279 <LOQ <LOQ <LOQ <LOQ LOQ limit o f quantitation (0.24 junol/kg) A naerobic L ive Sterile I Thursday, November OS, 2007 p. 257 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -257- Project No.: 112E-108 Wildlife International, Ltd. P ro ject N um ber 112E-108 Measured Concentrations of 7-2 s FTOH Qj.moL/kg) in Soils Treated withC Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______ L ive Sterile Mollisol DayO O 'O ' S3 VV Month 2 Month 6 Month 9 Month 12 Month L5 Month IS Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ LOQ = limit ofquantitation (0,24 pmol/kg) Tmndy, November 06, 2007 p. 258 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -258 - Project No.: 112E-108 Wildlife International, Ltd. Project Num ber 112E-108 Measured Concentrations of 7-2 s FTOH (junol/kg) in Soils Treated with^ ^ Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive Sterile Alfisol DayO Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit of quantitation (0.24 pmol/kg) . Thursday, November 08, 2007 p. 259 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -259- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 7-2 s FTOH (fimol/kg) in Soils Treated with ^ Sam ple A erobic A naerobic S o il Type_________ Interval_____________L ive Sterile_______L ive S terile O' O' SS VV Inceotisol DayO Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ 0.672 0.672 0.466 1.121 0.466 0.287 0.418 0.440 <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.24 pmol/kg) Thursday, November 08, 2007 p. 260 Company Sanitized. Does not contain T SC A CBI. W ildlife International, Ltd. - 260- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 7-3 Acid (junol/kg) in Soils Treated with ^ Sam ple A erobic A naerobic S o il Type_________ Interval_____________liv e Sterile_______L ive Sterile Ultisol DayO Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ 0.015 0.021 0.037 0.048 0.071 0.065 0.094 0.076 0.077 0.091 0.063 0.038 0.123 0.127 LOQ = limit o f quantitation (0.0045 pmol/kg) Wednesday, March 12,200S I p. 261 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -261 - Project No.: 112E-108 Wildlife International, Ltd._____________ Project Num ber 112E-108 Measured Concentrations of 7-3 Acid (pmol/kg) in Soils Treated with Sam ple S o il Type_________ In terva l A erobic A naerobic L ive_ _ S te tte _______ L ive Sterile Molllsol DayO Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 0.005 0.006 0.006 0.006 0.011 0.011 0.015 0.020 0.008 0.008 0.020 0.015 LOQ - limit o f quantitation (0.0045 pmol/kg) Wednesday, March 12,2008 p. 262 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 262- Project No.: 112E-108 Wildlife International, Ltd. Project N um ber 112E-108 Measured Concentrations of 7-3 Acid (pmoi/kg) in Soils Treated with^ 3 Sam ple A erobic A naerobic S o il Type_________ Interval____________ L ive Sterile_______L ive Sterile Alflsol Day 0 Month 2 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ 0.007 0.006 0.007 0.006 0.011 0.014 0.017 0.020 0.018 0.015 0.015 0.016 LOQ " limit of quantitation (0.0045 pmol/kg) Wednesday. March 12,200S p. 263 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -263 - ProjectNo.: 112E-108 Wildlife International, Ltd. Project N um ber U 2E-108 Measured Concentrations of 7-3 Acid (pmol/kg) in Soils Treated with. 1 Sam ple A erobic A naerobic S o il Type_________ In terva l_____________L ive Sterile_______L ive Sterile Inceotisol DayO Month 2 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ 0.038 0.022 0.153 0.185 0.290 0.347 0.626 0.557 0.590 0.655 0.638 0.480 0.803 0.728 LOQ = limit o f quantitation (0.0045 pmoi/kg) Wednesday, M ardi 13, 2009 p. 264 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -264- ProjectNo.: 112E-108 Wildlife International, Ltd. P ro je c tN u m b e rs ll2E -108, 109,110 Measured Concentrations of PFOA (pmol/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______ A erobic______ A naerobic Ultisol . DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <l o q <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ LOD = limit o f detection (0.0005 pmol/kg) LOQ = limit o f quantitation (0.004S pmol/kg) <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.023 0.019 0.021 0.022 0.022 0.022 0.025 0.025 0.029 0.028 0.026 0.025 0.022 0.024 0.018 0.017 95% 78% 86% 92% 92% 104% 103% 120% 114% 106% 104% 92% 100% 74% 68% 0.023 0.022 0.025 0.027 0.022 0.024 0.023 0.022 0.027 0.026 0,027 0.027 0,027 0.028 95% 92% 105% 113% 92% 98% 93% 90% 114% 108% 114% 113% 112% 115% i Friday, October 12, 2007 Page 1 o f4 p. 265 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -265- ProjectNo.: 112E-108 Wildlife InternationalLtd. Project Numbers 1i 2e - io 8 ,io 9 ,i 10 Measure^ Concentrations of PFOA (pmoi/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Molllsol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOD <LOD <LOD <LOD <L0D <LOD <LOQ <LOQ <LOD <LOD <LOQ <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ LOD = limit o f detection (0.0005 pmol/kg) LOQ - limit of quantitation (0,0048 pmol/kg) <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD F o rtified Recoveries A erobic A naerobic 0.022 0.022 0.023 0.024 0.021 0.023 0.022 0.022 0.026 0.027 0.032 0.029 0.032 0.029 0.028 0.028 0.022 0.025 89% 93% 97% 100% 88% 94% 91% 90% 107% m% 133% 131% 131% 120% 114% 116% 93% 104% 0.023 0.024 0.020 0.023 0.020 0.021 0.023 6.024 0.030 0.026 0.034 0.034 0.035 0.035 93% 101% 85% 96% 85% 89% 95% 99% 123% 109% 140% 139% 143% 143% Friday, October 12, 27 Page 2 o f4 p. 266 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 266- ProjectNo.: 112E-108 Wildlife International, Ltd. p ro ject N um bers 112E -108,109,110 Measured Concentrations of PFOA (pmol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l Untreated C ontrols A erobic A naerobic Alfisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD ' CLOD CLOD cLOD CLOD cLOD CLOD cLOQ cLOQ cLOD cLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD LOD = limit o f detection (0.0005 pmol/kg) LOQ = limit of quantitation (0.0048 pmolAg) cLOD cLOD cLOD CLOD CLOD cLOD cLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD F ortified R ecoveries A erobic A naerobic 0.022 0.020 0.022 0.024 0.024 0.023 0.024 0.024 0.026 0.026 0.026 0.028 0.027 0.027 0.028 0.030 0.022 0.022 92% 83% 89% 100% 99% 94% 100% 101% 110% 107% 108% 113% 110% 111% 115% 125% 92% 93% 0.022 0.020 0.021 0.022 0.023 0.024 0.025 0.025 0.025 0.026 0.027 0.026 0.025 0.027 92% 82% 87% 93% 96% 100% 106% 104% 104% 107% 111% 109% 105% 112% Friday, October 12, 27 Page S o f4 p. 267 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -267- ProjectNo.: 112E-108 Wildlife International, Ltd.__________________ P ro je ct N u m b ers 112E-108,109,11Q Measured Concentrations of PFOA (jrmol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic InceDtisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LO D = limit o f detection (0.0005 pmoi/kg) LO Q = limit o f quantitation (0.0048 pmol/kg) F ortified Recoveries A erobic A naerobic 0.025 0.025 0.031 0.024 0.026 0.026 0.030 0.027 0.027 0.028 0.032 0.026 0.029 0.026 0.035 0.030 0.023 0.023 104% 105% 129% 101% 106% 109% 124% 112% 113% 117% 134% 108% 121% 106% 145% 124% 96% 96% 0.023 0.023 0.026 0.024 0.026 0.025 0.026 0.026 0.029 0.027 0.026 0.028 0.027 0.028 97% 94% 109% 98% 108% 105% 107% 109% 119% 111% 106% 115% 113% 116% i Friday, October 12, 2007 Page 4 o f 4 p. 268 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -268 - Project No.: 112E-108 Wildlife International, Ltd. Project N um bers U 2E -108,109,110 Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month $ Month 12 Month 13 Month IS Month 24 <LOQ <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD LOD = limit of detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0044 (imol/kg) CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD c LOD CLOD CLOD F ortified R ecoveries A erobic A naerobic 0.016 0.014 0.017 0.017 0.015 0.015 0.016 0.016 0.019 0.017 0.014 0.016 0.006 0 .0 1 2 0.011 0.009 72% 65% 77% 79% 69% 69% 72% 72% 89% 80% 65% 73% 29% 54% 51% 40% 0.017 0.016 0.017 0.019 0.018 0.018 0.018 0.017 0 .0 2 0 0.017 0.018 0.017 0.014 0.015 80% 74% 77% 87% 84% 82% 82% 79% 93% 76% 82% 80% 65% 70% ' Friday, October 12,2007 Page 1 o f4 p. 269 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -269- Project No.: 112E-108 Wildlife International, Ltd. P rojectN um bersll2E -108,109,110 Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______Anaerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1$ Month 18 Month 24 CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD <LOD <LOD CLOD CLOD CLOD CLOD cLOQ cLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD 0.020 92% 0.020 90% 0.018 81% 0.019 83% 0.020 91% 0.021 93% 0.020 90% 0.020 91% 0.019 88% 0.019 87% 0.022 100% 0.020 94% 0.022 102% 0.018 82% . 0.017 78% 0.020 90% 0.013 59% 0.014 03% 0.016 0.019 .0.017 0.019 0.017 0.021 0.018 0.018 0.021 0.017 0.022 0.023 0.022 0.019 73% 88% 77% 87% 77% 98% 80% 80% 97% 78% 99% 107% 100% 83% LOD = limit o f detection (0.0003 junol/kg) LO Q = limit o f quantitation (0.0044 pmol/kg) Friday. October 12i 2007 Page 2 c f4 p. 270 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -270- Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112B-108,109,110 Measured Concentrations of 8-2 FTUA (pmol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic F ortified R ecoveries A erobic A naerobic Alsol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD cLOD CLOD CLOD cLOD CLOD cLOD cLOD cLOD cLOD cLOD CLOD cLOD cLOD CLOD CLOD CLOD CLOD <LOD CLOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD <LOD CLOD CLOD cLOD cLOD CLOD cLOD CLOD 0.018 0.017 0.019 0.021 0.023 0.017 0.016 0.020 0.021 0.020 0.014 0.020 0,018 0,020 0.018 0.024 0.014 0.015 84% 77% 85% n% 105% 76% 75% 92% 96% 91% 66% 90% 84% 90% 80% 111% 62% 67% 0.020 0.016 0.020 0.021 0.020 0.019 0.019 0.020 0.024 0.024 0.018 0.018 0.023 0.018 89% 75% 93% 95% 92% 89% 86% 94% 109% 108% 81% 83% 103% 83% LOD = limit of detection (0.0003 pmol/kg) LOQ - limit o f quantitation (0.0044 pmol/kg) Friday, October 13, 307 Page 3 o f4 p. 271 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -271 - Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 1 1 2 E -1 0 8 ,1 0 9 .1 10 Measured Concentrations of 8-2 FTUA (fimol/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic F ortified R ecoveries A erobic A naerobic Incentisol D ayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD LOD = limit of detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0044 pmol/kg) CLOD CLOD cLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD 0.024 0.021 0.024 0.018 0.019 0.021 0.020 0.017 0.018 0.020 0.022 0.018 0.015 0.015 0.016 0.014 0.012 0.011 u im 96% 108% 83% 88% 97% 94% 79% 80% 89% 101% 81% 70% 68% 74% 64% 53% 53% 0.017 0.018 0.018 0.016 0.019 0.017 0.017 0.018 0.017 0.019 0.014 0.015 0.013 0.016 79% 81% 83% 73% 85% 78% 77% 82% 76% 88% 66% 70% 61% 71% Friday, October 12, 2007 Fage 4 o f4 p. 272 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. - 212- Project No.: 112E-108 Wildlife International, Ltd. P ro ject N um bers 112E -108,109,110 Measured Concentrations of 8-2 FTA (pmol/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic______ A naerobic Ultisol Day 0 Weekl Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD cLOD CLOD <LOQ cLOQ cLOD cLOD CLOD LOD = limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg) CLOD CLOD CLOD cLOD CLOD CLOD cLOD cLOD cLOD CLOD cLOD CLOD cLOD cLOD 0.013 0.012 0.013 0.014 0.011 0.011 0.011 0.011 0.007 0.005 0.007 0.008 cLOQ CLOQ 0.009 CLOQ 61% 58% 62% 69% 54% 55% 54% 53% 33% 24% 34% 37% 41% 0.014 0.014 0.015 0.015 0.013 0.014 0.011 0.013 0.015 0.011 0.010 0.010 0.007 0.007 67% 66% 70% 69% 61% 65% 53% 63% 72% 51% 48% 46% 35% 32% Friday, October 12, 2007 Page 1 o f4 p. 273 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -273 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E - 108, 109,110 Measured Concentrations of 8-2 FTA (funol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic F ortified R ecoveries A erobic A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD 0.006 CLOD CLOD CLOD <LOQ CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOQ cLOD CLOD <LOD CLOD cLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD 0.018 0.019 0.018 0.019 0.019 0.018 0.017 0.017 0.016 0.017 0.017 0.014 0.013 0.013 0.010 0.013 0.005 CLOQ rm 90% 84% 90% 90% 88% 83% 81% 78% 79% 84% 09% 64% 62% 50% 62% 23% 0.015 0.018 0.015 0.016 0.013 0.017 0.013 0.015 0.017 0.015 0.012 0.014 0.012 0.011 70% 87% 71% 76% 64% 83% 63% 70% 80% 71% 58% 65% 57% 52% LOD - limit o f detection (0.0011 pmol/kg) LOQ = limit o f quantitation (0.0042 pmol/kg) Friday, October 12, 2007 Page 2 o f4 p. 274 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -274- Project No.: 112E-108 Wildlife International, Ltd. Project Num bers 112E-108,109,110 Measured Concentrations of 8-2 FTA (iinol/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______ A erobic_______A naerobic Alfisol . Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month IS Month 24 <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOQ cLOQ 0.016 0.017 0.016 0.018 0.022 0.018 0.014 0.017 0.016 0.015 0.009 0.014 0.013 0.014 0.011 0.016 0.008 0.008 78% 79% 78% 84% 106% 87% 68% 84% 75% 70% 42% 67% 60% 66% 51% 77% 40% 37% 0.018 0.014 0.018 0.018 0.018 0.017 0.016 0.017 0.018 0.017 0.014 0.014 0.015 0.015 88% 67% 85% 84% 84% 81% 77% 83% 88% 83% 67% 66% 73% 70% . LOD " limit o f detection (0.0011 pmol/kg) LOQ s limit o f quantitation (0.0042 pmol/kg) Friday. October 12, 2007 P aget o f4 p. 275 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -275 - Project No.: 112E-108 Wildlife International, Ltd. Project Num bers 112E-108,109,110 Measured Concentrations of 8-2 FTA (unol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic F ort\fled Recoveries A erobic A n a ero b ic InceDtisol - Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 M onths Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <LOD CLOD cLOD CLOD CLOD CLOQ cLOD CLOD CLOD cLOD cLOD CLOD CLOD cLOQ CLOQ cLOQ CLOD cLOD CLOD cLOQ cLOD cLOD CLOD cLOD CLOD CLOD <LOD <LOD cLOD CLOD CLOD CLOD cLOD CLOD 0.022 0.020 0.020 0.016 0.015 0.018 0.015 0.013 0.011 0.012 0.011 0.009 0.009 0.008 0.007 0.007 0.004 CLOQ 107* 96% 98* 76% 70% 84% 71% 03% 53% 58% 53% 45% 43% 37% 34% 34% 21% 0.016 0.015 0.015 0.014 0.015 0.016 0.012 0.014 0.010 0.013 0.008 0.007 0.005 0.007 78% 72% 70% $7% 72% 76% 59% 66% 48% 63% 36% 34% 26% 35% L O D - limit o f detection (0.0011 pmol/kg) L O Q = limit o f quantitation (0.0042 pmol/kg) F rid a y, O ctober 12, 2 0 0 7 Page 4 o f4 p. 276 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -276- Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 1 12E-108,109,110 Measured Concentrations of PFNA (jimol/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified Recoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic______ A naerobic Ultisol . DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.020 0.017 0.021 0.021 0.021 0.021 0.022 0.022 0.024 0.024 0.022 0.023 0.015 0.022 0.017 0.014 94% 78% 97% 98% 95% 98% 104% 101% 114% 114% 103% 106% 70% 103% 79% 67% 0.022 0.021 0.021 0.023 0.021 0.022 0.022 0.021 0.024 0.024 0.025 0.025 0.023 0.025 100% 99% 99% 107% 97% 103% 103% 96% 113% 110% 116% 117% 108% 116% LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) Friday, October 12,2007 Page 1 o f4 p. 277 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -277- Project No.: 112E-108 Wildlife International, Ltd. Project Num bers 112E-108,109,110 Measured Concentrations of PFNA (tmol/kg) in Untreated and Fortified Soils S o il Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month IS Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit o f detection (0.0003 pmol/kg) LOQ =*limit o f quantitation (0.0043 pmol/kg) F ortified Recoveries A erobic A naerobic 0.020 0.021 0.021 0.021 0.021 0.021 0.022 0.018 0.023 0.023 0.026 0.026 0.026 0.025 0.022 0.024 0.017 0.018 94% 96% 97% 97% 93% 99% 103% 86% 107% 106% 122% 119% 130% 117% 100% 113% 81% 83% 0.021 0.022 0.019 0.021 0.021 0.021 0.020 0.021 0.023 0,022 0.025 0.028 0.026 0.026 96% 101% 89% 98% 96% 99% 93% 98% 107% 101% 116% 138% 133% 119% Friday, October 12, 2007 Page 2 o f4 p. 278 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd, -278- Project No.: 112E-108 Wildlife International, Ltd. Project Num bers 112E-108,109,110 Measured Concentrations of PFNA (funol/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l Untreated Controls A erobic Anaerobic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month? Month 12 Month 1$ Month 18 Month 24 CLOD CLOD cLOD CLOD CLOD CLOD <LOD CLOD cLOD CLOD CLOD cLOD cLOQ cLOQ cLOQ CLOQ cLOQ cLOQ <LOD CLOD <LOD LOD = limit o f detection (0.0003 pmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD cLOQ cLOQ F o rtified Recoveries A erobic A n a ero b ic 0.021 0.020 0.020 0.024 0.022 0.020 0.022 0.023 0.024 0.024 0.022 0.025 0.023 0.025 0.024 0.026 0.019 0.018 98% 93% 94% 112% 103% 91% 103% 107% 113% 110% 100% 114% 108% 114% 113% 123% 87% 86% 0.021 0.017 0.020 0.022 0.021 0.022 0.022 0.022 0.024 0.024 0.023 0.023 0.025 0.022 97% 81% 94% 101% 98% 101% 103% 100% 110% 110% 107% 107% 115% 102% Friday, October 12$ 2007 i Page 3 o f4 p. 279 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -279- Project No. : 112E-108 Wildlife International, Ltd. ProjectN um bersll2E-108,109,110 Measured Concentrations of PFNA (pmol/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______A naerobic Inceotisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month? Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOQ <LOD <LOQ <LOQ <LOQ cLOQ <LOQ <LOQ cLOQ <LOQ <LOD <LOQ CLOD CLOD CLOD CLOD CLOD cLOQ CLOQ cLOQ cLOQ cLOQ CLOD cLOQ CLOQ cLOQ <LOQ 0.027 0.02S 0.028 0.023 0.023 0.023 0.027 0.023 0.024 0.026 0.030 0.023 0.022 0.021 0.028 0.023 0.019 0.019 124% 116% 130% 106% 107% 107% 125% 107% m% 119% 140% 103% 104% 100% 130% 109% 89% 88% 0.022 0.022 0.023 0.021 0.022 0.022 0.022 0.023 0.024 0.024 0.024 0.023 0.022 0.023 102% 100% 108% 98% 103% 102% 101% 107% 111% 111% 110% 108% 101% 108% LOD = limit o f detection (0.0003 (jmol/kg) LOQ = limit o f quantitation (0.0043 pmol/kg) Friday, October 12, 2007 Page 4 o f4 p. 280 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -280- Project No.: 112E-108 Wildlife International, Ltd._________________P ro je c t N u m b ers 1 1 2 E -1 0 8 ,I0 9 ,1 1 0 Measured Concentrations of PFDA (jwnol/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______ A erobic______ Anaerobic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOD <LOQ <L0Q <LOQ <L0Q <LOQ <L0D <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ cLOQ <LOQ 0.018 0.014 0.017 0.017 0.017 0.017 0.018 0.018 0.021 0.021 0.019 0.019 0.010 0.018 0.016 0.014 93% 74% 89% 86% 88% -89% 94% 93% 109% 109% 96% 100% 52% 95% 84% 70% 0.018 0.018 0.018 0.019 0.018 0.018 0.018 0.016 0.020 0.020 0.021 0.021 0.019 0.020 92% 91% 90% 97% 94% 92% 93% 80% 104% 102% 110% 109% 96% 104% LOD = limit o f detection (0.0002 fimol/kg) LOQ = limit o f quantitation (0.0039 uraol/kg) Friday, October 12, 2007 Pag* 1 o f4 p. 281 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -281 - Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E - 108, 109,110 Measured Concentrations of PFDA (fimol/kg) in Untreated and Fortified Soils Sam ple U ntreated C ontrols S o il Type_________ Interval_______ A erobic A naerobic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ cLOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg) F ortified R ecoveries A erobic A naerobic 0.019 0.019 0.019 0.020 0.019 0.020 0.019 0.016 0.019 0.019 0.023 0.021 0.024 0.022 0.018 0.020 0.015 0.016 100% ioq% 98% 104% 97% 101% 98% 81% 99% 97% 117% 109% 121% 112% 92% 102% 76% 80% 0.018 0.019 0.016 0.018 0.018 0.018 0.017 0.018 0.020 0.018 0.022 0.024 0.022 0.021 92% 99% 82% 95% 92% 93% 90% 90% 104% 92% 114% 121% 112% 109% ' Friday, October 12$ 2007 Page 2 o f4 p. 282 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -282- Project No.: 112E-108 Wildlife International, Ltd. P roject N um bers 112E -108,109,110 Measured Concentrations of PFDA (p.moL/kg) in Untreated and Fortified Soils ' iSam ple U ntreated Controls F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic______ A naerobic Aifisol Day 0 Week 1 Week 2 Week 4 Months Month. 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOD <LOD <LOD <LOD LOD = limit of detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0039 ftmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ 0.018 0.018 0.018 0.021 0.020 0.017 0.018 0.020 0.023 0.022 0.018 0.021 0.021 0.022 0.021 0.026 0.018 0.018 93% 93% 94% 110% 101% 86% 90% 103% 119% 112% 93% 109% 107% 112% 109% 133% 92% 93% 0.018 0.014 0.017 0.018 0.018 0.019 0.019 0.019 0.022 0.022 0.018 0.019 0.023 0.017 92% 74% 87% 93% 90% 95% 97% 96% 113% 114% 90% 96% 116% 86% Friday, October 13, 3007 Page S o f4 p. 283 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -283 - project n 0.: 112E-108 ,WiIdlif e International Ltd.__________________ P ro je c t N u m b ers 112E -108,109,110 Measured Concentrations of PFDA (fimol/kg) in Untreated and Fortified Soils Soil Type S a nale In te r v a l U ntreated C ontrols A erobic A naerobic Incentisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOD = limit o f detection (0,0002 pmol/kg) LOQ = limit o f quantitation (0.0039 pmol/kg) F ortified Recoveries A erobic A naerobic 0.027 0.024 0.028 0.023 0.022 0.022 0.024 0.022 0.023 0.026 0.027 0.021 0.021 0.018 0.025 0.021 0.018 0.018 139% 124% 14696 11696 11396 11696 12196 11596 12096 13596 14196 10396 107% 92% 123% 109% 93% 92% 0.019 0.019 0.021 0.019 0.021 0.020 0.020 0.021 0.023 0.024 0.021 0.023 0.019 0.021 99% 9796 107% 96% 101% 105% 101% 107% 118% 122% 108% 116% 96% 106% Friday, October 13, 2007 JPug*4of4 p. 284 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -284- Project No.: 112E-108 Wildlife International, Ltd._________________P ro je ct N um bers 1 1 2 E -1 0 8 ,109,110 Measured Concentrations of PFUA (pmol/kg) in Untreated and Fortified Soils Sam ple Untreated C ontrols F ortified R ecoveries S o il Type_________ Interval_______ A erobic A naerobic______A erobic_______A naerobic U ltisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOD <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ 0.015 0.012 0.015 0.014 0.013 0.014 0.015 0.013 0.018 0.018 0.016 0.017 0.007 0.015 0.013 0.011 85% 69% 83% 82% 76% 78% 86% 74% 102% 101% 92% 93% 41% 84% 72% 62% 0.015 0,016 0.015 0.015 0.016 0.015 0.016 0.012 0.017 0.017 0.017 0.017 0.016 0.018 85% 88% 85% 84% 88% 86% 91% 70% 98% 95% 94% 95% 88% 99% LOD - limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) Friday, October 12, 2007 Page 1 o f4 p. 285 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -285- projectNo.: 1 1 2 E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 112E - 108, 1 0 9,110 Measured Concentrations of PFUA (fimol/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l U ntreated C ontrols A erobic A naerobic Mollisol DayO 'Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD LOD = limit of detection (0.0002 pmol/kg) LOQ " limit o f quantitation (0.0036 pmol/kg) F ortified Recoveries A erobic A naerobic 0.017 0.017 0.015 0.017 0.016 0.016 0.015 0.012 0.015 0.015 0.018 0.018 0.017 0.015 0.013 0.016 0.011 0.011 95% 96% 87% 93% 90% 92% 87% 69% 84% 84% 101% 101% 95% 87% 74% 88% 61% 63% 0.015 0.015 0.012 0.014 0.011 0.013 0.011 0.014 0.015 0.013 0.017 0.017 0.017 0.014 82% 86% 67% 76% 63% 71% 63% 80% 8S% 75% 96% 94% 98% 79% Friday, October 12, 2007 Pag* 2 o f4 p. 286 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -286- Project No.: 112E-108 Wildtife International, Ltd._________________ P ro je ct N u m b ers 112E -108>109,110 Measured Concentrations of PFUA (junol/kg) in Untreated and Fortified Soils Sam ple S o il Type_________ Interval U ntreated Controls A erobic A naerobic ' Alfisol DayO Week 1 Week 2 Week 4 Month 2 Mondi 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD <LOD <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ <LOD <LOD <LOD LOD = limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ F o rtified Recoveries A erobic_______A naerobic 0.016 0.016 0.016 0.019 0.017 0.014 0.014 0.017 0.018 0.017 0.013 0.016 0.016 0.016 0.015 0.022 0.016 0.016 92% 87% 92% 109% 94% 60% 77% 94% 103% 97% 73% 89% 89% 93% 86% 122% 87% 88% 0.015 0.011 0.015 0.015 0.014 0.014 0.012 0.013 0.016 0.017 0.013 0.013 0.017 0.012 83% 61% 83% 86% 79% 79% 70% 76% 88% 99% 74% 72% 93% 67% Friday> October 13, 3007 Page 3 o f4 p. 287 Company Sanitized. Does not contain TSC A CBI. W ildlife International, Ltd. -287- projectno.: 1 1 2 E-108 WiIdlife International, Ltd.__________________P ro je ct N u m b ers U 2 E -1 0 8 ,1 0 9 ,1 1 0 Measured Concentrations of PFUA (fimol/kg) in Untreated and Fortified Soils ~ Sam ple U ntreated C ontrols F ortified Recoveries S o il Type_________ In te r v a l______ A erobic A naerobic______ A erobic_______Anaerobic Inceotisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOQ <LOQ cLOQ <LOQ <LOQ <LOQ <LOD <LOD <LOD <LOD <LOD CLOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD cLOQ cLOQ 0.021 0.020 0.022 0.018 0.016 0.018 0.017 0.016 0.018 0.021 0.022 0.016 0.014 0.015 0.018 0.015 0.013 0.012 120% 112% 125% 101% 92% 103% 98% 89% 104% 117% 122% 88% 82% 83% 99% 82% 74% 69% 0.014 0.013 0.014 0.013 0.013 0.015 0.014 0.015 0.015 0.018 0.012 0.013 0.012 0.015 79% 74% 79% 74% 74% 87% 80% 85% 87% 103% 69% 73% 70% 85% LOD - limit o f detection (0.0002 pmol/kg) LOQ = limit o f quantitation (0.0036 pmol/kg) Friday. October 12, 2007 Page 4 o f4 p. 288 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. - 288- Project No.: 112E-108 Wildlife InternationalLtd. P roject N um bers 112E -108,109,110 Measured Concentrations of 8-2 FTOH Qimol/kg) in Untreated and Fortified Soils Soil Type Sam ple In te r v a l U ntreated Controls A erobic A naerobic F ortified Recoveries A erobic A naerobic Ultisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD <L0D <LOD <LOD <LOD <LOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD 0.622 0.609 0.525 0.531 0.452 0.448 0.356 0.317 0.220 CLOQ CLOQ <LOQ CLOQ CLOQ CLOQ CLOQ CLOQ CLOQ 115% 113% 97% 99% 84% 83% 66% 59% 41% 39% 18% 21% 16% 9% 9% 0% 0% 0% 0.627 0.621 0.475 0.555 116% 115% 88% 103% 0.536 0.390 0.385 0.334 0.356 0.253 CLOQ <LOQ cLOQ 99% 72% 71% 62% 66% 47% 35% 22% 27% LOD " limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg) I Monday. October 25, 2007 Page1 o f4 p. 289 Company Sanitized. Does not contain TSCA CB1. W ildlife International, Ltd. -289- ProjectNo.: 112E-108 Wildlife International, Ltd. P ro je ctN u m b ersll2 E -1 0 8 (109,110 Measured Concentrations of 8-2 FTOH (pmol/kg) in Untreated and Fortified Soils S a m p le U n trea ted C o n tro ls F o rtifie d R eco veries S o il T y p e ____________ In te r v a l_________ A e r o b ic A n a e r o b ic _______ A e r o b ic ________ A n a e r o b ic Mollisot Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD 0.645 120% 0.562 0.459 0.469 0.419 0.241 0.256 cLOQ cLOQ CLOQ CLOQ cLOQ CLOQ CLOQ CLOQ cLOQ CLOQ 104% 85% 87% 78% 4S% 48% 21% 25% 7% 13% 3% 4% 0% 3% 0% 0% 0.574 0.602 0.526 0.589 0.554 0.524 0.412 0.413 CLOQ 0.349 cLOQ CLOQ CLOQ CLOQ 104% 112% 98% 109% 103% 97% 77% 77% 40% 65% 28% 18% 14% 6% LOD = limit o f detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg) . Monday, October IS, 2007 Page 2 o f4 p. 290 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -290- Project No.: 112E-108 Wildlife International, Ltd. Project N um bers 112E-108,109,110 Measured Concentrations of 8-2 FTOH (funol/kg) in Untreated and Fortified Soils S a m p le S o il T y p e ___________ I n te r v a l U n trea ted C o n tro ls A e ro b ic A n a e ro b ic F o r tifie d R eco veries A e r o b ic ________ A n a e r o b ic AlfisoJ DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month!) Month 12 Month IS Month 18 Month 24 cLOD <LOD <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD cLOD cLOD 0.662 0.527 0.453 0.309 0.336 0.307 0.252 0.241 cLOQ CLOQ cLOQ cLOQ cLOQ CLOQ cLOQ CLOQ cLOQ cLOQ 123% 98% 84% 57% 62% 57% 47% 43% 30% 32% 22% 21% 5% 12% 4% 7% 0% 0% 0.574 0.548 0.462 0.437 0.441 0.347 0.274 0.308 <LOQ CLOQ cLOQ CLOQ cLOQ cLOQ 107% 102% 86% 81% 82% 64% 51% 57% 33% 23% 20% 15% 4% 7% LOD limit o f detection (0.048 junol/kg) LOQ = limit o f quantitation (0.216 nmol/kg) Monday, October IS, 2007 Page 3 o f4 p. 291 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -291 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 112E-108,109,110 Measured Concentrations of 8-2 FTOH Qunol/kg) in Untreated and Fortified Soils Soil Type S a m p le In te r v a l U n trea ted C o n tro ls A erobic A naerobic F o r tifie d R eco veries A erobic A naerobic Incentisol D ayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month IS Month 18 Month 24 <LOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD CLOD cLOD CLOD cLOD CLOD cLOD CLOD CLOD cLOD cLOD CLOD 0.613 0.565 0.575 0.613 0.529 0.505 0.470 0.612 0.434 0.430 0.338 0.401 cLOQ cLOQ cLOQ 0.248 cLOQ cLOQ 114% 103% 107% 114% 08% 94% 87% 114% 81% 80% 63% 74% 34% 34% 32% 46% 0% 0% 0.619 0.613 0.555 0.560 0.511 0.507 0.495 0.471 0.289 0.356 0.228 0.228 cLOQ CLOQ 115% H4% 103% 104% 95% 94% 92% 87% 54% 66% 42% 42% 17% 15% LOD = limit of detection (0.048 pmol/kg) LOQ = limit o f quantitation (0.216 pmol/kg) I Monday, October IS, 3007 Page 4 of4 p. 292 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -292- Project No.: 112E-108 Wildlife InternationalLtd. Project N um bers m E -1 0 8 ,1 0 9 ,1 1 0 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils S a m p le U ntreated C o n tro ls F o rtifie d S o ils S o il T y p e ____________I n te r v a l_________ A e r o b ic A n a e r o b ic _______ A e r o b ic A n a e r o b ic Ultisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Months Month 9 Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L o f headspace) <LOQ <LOQ <LOQ <LOQ <loq <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 1 o f4 p. 293 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -293 - Project No.: 112E-108 Wildlife International, Ltd. Project N um bers 112E-108.109, 110 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils S o il T ype S a m p le In te rv a l U n trea ted C o n tro ls A e ro b ic A n a e ro b ic Mollisol DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month? Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L ofheadspace) F o rtifie d S o ils A e ro b ic A n a e ro b ic <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 2 o f4 p. 294 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -294- Project No.: 112E-108 Wildlife International, Ltd. P ro je c t N u m b e rs 112E-108,109,110 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils S o il T ype S a m p le In te rva l U n trea ted C o n tro ls A e ro b ic A n a ero b ic Alfisol Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 1$ Month 18 Month 24 <LOQ <l o q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ = limit o f quantitation (0.027 pmol/L o f headspace) F o rtifie d S o ils A e ro b ic A n a e ro b ic <LOQ <L0Q <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ Monday, October IS, 2007 Page 3 o f4 p. 295 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. -295 - Project No.: 112E-108 Wildlife International, Ltd. Project Numbers 1123-108,109,110 Measured Concentrations of 8-2 FTOH (pmol/L) in Headspace Gases from Untreated and Fortified Soils S o il T ype S a m p le In te rv a l U n trea ted C o n tro ls A e ro b ic A n a e ro b ic Incentisot DayO Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 M onth? Month 12 Month 15 Month 18 Month 24 <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ LOQ *limit o f quantitation (0.027 jimol/L o f headspace) ' F o rtifie d S o ils A e ro b ic A n a e ro b ic <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L0Q <LOQ <L0Q <LOQ ! Monday, October IS, 2007 Page 4 o f4 p. 296 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -296- Appendix III Example Calculations A. Standard Curves by Linear Regression Standard curves were prepared by plotting the ratio of analyte concentration (pg/L = ng/mL) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid to the internal standard concentration (0.75 ng/mL 2-13C PFOA for 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA and 7-3 Acid and 100 ng/mL HDFD for 8-2 FTOH and 7-2 sFTOH) in solutions with known concentrations on the abscissa and the respective peak area responses on the ordinate. Weighted regression (1/x) was applied to analysis of the data to determine the equation with respect to the abscissa as shown below: A = [m x (C /Q ) + b] x Aj where A = A) = m= C= Ci = b= analyte area internal standard area slope analyte concentration (ng/mL) internal standard concentration (ng/mL) intercept Concentrations of analytes in the final solutions of processed samples, just prior to injection, were calculated using a rearrangement of the above equation: c _ C;x(A/Aj) -b m B. Residue Concentrations of 8-2 FTOH. 8-2 FTA. 8-2 FTUA, PFOA. PFNA. PFDA. PFUA. 7-2 sFTOH and 7-3 Acid in Soil Extracts Residue concentrations (pg/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation: Sample Concentration in pg/kg = y r-& & (W) x (V^il) n\ X \(rVi2) where C = Concentration (ng/mL) as determined above Ve = Extraction volume (125 mL) V,i = First initial volume (mL) Vi2 = Second initial volume (if required) Vn = First final volume (mL) Vr = Second final volume (mL) W = Weight of sample (25 g dry weight). p. 297 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Project No.: 112E-108 -297- Residue concentrations (pmol/kg) of 8-2 FTOH, 8-2 FTA, 8-2 FTUA, PFOA, PFNA, PFDA, PFUA, 7-2 sFTOH and 7-3 Acid in soil extracts were determined using the following equation: Sample Concentration in pmol/kg = C x molecular weight (pmol/pg) where C = Sample concentration (ng/kg) as determined above C. Procedural Recoveries in Fortified Controls Procedural recoveries were calculated for fortified control samples prepared in each test condition at known concentrations in 8-2 FTOH (250 pg/kg), 8-2 FTA (10.00 pg/kg), 8-2 FTUA (10.00 pg/kg), PFOA (10.00 pg/kg), PFNA (10.00 pg/kg), PFDA (10.00 pg/kg) and PFUA (10.00 pg/kg). Percent recoveries of die each analyte were determined to provide confirmation that the method was performing adequately. Percent recoveries were determined by dividing the concentration of the analyte recovered in the fortified sample by the nominal concentration added as shown below: Percent Recovery - ,, 100% pg/kg Added p. 298 Company Sanitized. Does not contain TSCA CBI. W ildlife International, Ltd. Sampling Interval Day 0 Week 1 Week 2 Week 4 Month 2 Month 4 Month 6 Month 9 Month 12 Month 15 Month 18 Month 24 -298 Appendix IV Duration of Incubation Actual Days of Incubation Alfisol 0 7 14 . 28 63 119 182 273 365 462 546 728 Inceptisol 0 7 14 28 63 119 182 273 365 462 546 728 Ultisol 0 7 14 28 56 119 182 273 364 462 553 728 Project No.: 112E-108 Mollisol 0 7 14 28 56 119 182 273 364 462 553 728