Document 99nXZJXVGjyBOpq0LVbxpKepL

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 2052.The testsample is FC-206A. Currentinformationindicateistisa mixtureof0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% Hydroxy foamer, 1.75% sodium octylsulfate1,.2% poloxyethylenemonooctylphenylether, 0.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlectthe toxicityof the fluorochemical component of the testsample. METHOD Method: 3M EnvironmentalLaboratoryProtocolforDeterminingAcute Toxicityofa Compound toActivatedSludge GLP: No Year Completed: 1979 Analyticalmonitoring: Dissolvedoxygen concentrabons. Statisticamlethods: Not given. Test organism source: Acbvatedsludgemixed liquocrollectefdromthe MetropolitaWnastewater Treatment Plant(Pig'sEye Plant),St.Paul,MN. Test condition: Dilutionwater: Deionizedwater. Reference and testsolutionpreparation:A primarystocksolution was createdby dilutin0g.60 g testsample to 100 mL withdilution water. Test vessels: Not given. Number of concentrations: one plus 1 blankcontrol Temperature: Not given. Total suspended solids and pH on day of testing:Not given. Element Basis: Respirationinhibitioans determined by oxygen consumption. RESULTS Nominal concentrations: 1 Blank controland 1000 mg/L Element values: No acute inhibitoreyffectson acbvated sludge respiration rateat1000 mg/L witha contacttimeof5 minutes. Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplyto thatmixtureand not thefluorochemicalcomponent alone. CONCLUSIONS The testsample exhibitedno inhibitoreyffectsat 1000 mg/L overa 5-minute exposure period. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. Study lacksdocumentation.The inoculumis not properlycharacterizedT.here isno characterizationfthetestsubstance purityand the studylacksanalyticaclonfirmationoftheamount of fluorochemicalproportioninsolution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory,St Paul,MN, Lab Request number 4970S, 1979. OTHER Last changed: 6/26/00 Pon$W IV$"-e-owo ENVIRONMENTAL SAMPLE DESCRIPTION -2-0(o,41 4orlib-02 ri t&4 File:-fop No. ENOR. UM3 - WORK SHEET Dow: Analyst:L Hri.: .70 pi I-vao OXYGEAI e-OAe OIV Al ijutl ))PS- Y.,o e-"7, I -7,5-S- 7.0 3 1-7 3, 3.5T /1 @ o,SS7 .2,q v Reviewed by: UMMARY NEET I fI LP Z Iw auZ Z lm W ix Cl ci 2 7- tI r-r I T-T- Ir +Hi I I I ITT t t -T-T-T-rr . . I Ir T UT-T- if -r-TT-- -2N -4: -Li I =FTT-T- -FTT-F I ITT II -FT-r I ---r-TT- T -JLI I I 4- -r.t-t IT IT . -T-T- iiv [it I I I I I IL 1 7t7 tt t t-t -i 0-' 17T 2 b TIME minute$ 3M ENVIPONMENTAL LAFIORATO!' -ICTOCOL FOR I)ETERNINING ACUTE TOXICITY OF A COWO.A TO ACTIVATED SLUDGE ,E@(X-*EDURE .1 Ohtain 4 liters of activated sludge from aeration basin at the Metro Wastewater Treatment plant. .2 Use sludge within 4 hours of collection. .0 Prepare a 100-al, 6,000-ft/I stock solution of the material to be tested in air-maturated, deionized (DI) water. .0 Starting with this stock'solution, make-sorial dilutions by transferring 10 al'of the pro-. ceding solution into 2:00-alvolmetr:Lc fl&Wm and bringing to volume with &JLr-saturated, water. In this mmnor, prepare 600-, 60-, O-P and 0.6-mg/l solutions .1 Prepare a 1,200 mg/l n@trient broth-solution by dissolving 1.2 g in approximately 100 al of DI water at 95 C and diluting to I liter with air-saturated water. ,2 Aerate to bring to air saturation-. .1-Pass the sludge through a 100-mah stainless steel *Lev*. .2 Place 3 liters of*tbo activated sludge le a .4-literb*&"r. S.Placo the.b*Wmr on an Insulating sheot on I top of a magnetic mixer. .4 Oot the mixer speed so that It keeps tb* -"sludge comietoly suspended. .A.Aerate the sludge rapidly to bring@it to .near aJLrsaturation. :t@,Plaao*a 1-in. magnetic stirring bar In a bottle. -1-Placo the BW bottle an a sb'oetof foam -,,Awmlatioaan top of a magnetic mi"r. 3 Add'50.nl of nutrient broth solution and 50 al of aerated DI water. 4 Start the mixer at a rate that will keep sludge suspended. 5 Transfer enough of the completely dis- persed, &*rated, activated aludge.to fill the BOD'bottlo using &.500-i-olbeaker. MATBRIALS AND REAGENTS . Th o follov*lng materials for this procedure: and reagents IL)Oxygen raeter 2) DO probe 3) AM 4) 4,'Literbeaker 5) 500 mi beaker 6) 10 ml pipets 7) 100 ml volumetric flasks 8) Compressed air 9) Air spargers 10) Plastic tubing 11) 300 ml BOD 12) 3-in. Magn 13) 1-in. Magn 14) Magnetic m 15) 3/4 in. Fo 16) Stopwatch 17) 100-mesh a 18) Deionized 19) Nutrient b 20) Activated 6.6 Start the stopwatch @immediately. 6.7 Insert the DO probe into the BOD record the time and DO concentrat quent intervals for 10 min or un concent' ration reaches 0.5 mg/l. 6.8 Obtain fresh sludge if the DO is - 0.5 mg/l at 10 min. 6.9 If the DO is depleted to 0.5 mg/l 5 min., bring the time required f uptake to between 5 and 10 min b sludge stock with air-saturated D 7.1 Repeat the procedure described in 6.7, except substitute 50 ml of t solutions or aerated DI water con aerated DI water used in step 6.3 7.2 Run the 0 assays in the followin 1) 6,000-?Ag/ltest material, 2) D 3) 0.1 mg/l, 4) 100 mg/l, 5) 1 mg No. 1, 7) 10 mg/l. 8.1 Use a full sheet of graph paper t concentration as a function of ti and each test concentration. 8.2 Label each plot with the final co test material in each BOD bottle. tions are 1,000, loo, 10, 1, and