Document 99XRdB3kY7xwLEdMXR7E2vBKq

ARZR6 -- 0815 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST EST SUBSTANCE Identity: ArefmeirxrteudretocaonstaPiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m2a0y6CaEls.o b(1eOctanesulfonic acid) (CAS # 2795-393). R20e6mCaEr.ksC:urreTnhtein3fMorpmratoidounctiinodniclaottensuimtbisear wmiaxstu5r0e2.ofT0h.e92t%estPFsOamSp,l1e5is%FCd1i5et5h%ylsenoedigluymcoolctbyultysluleftahtee,r,17.60.50%0p%owlaytoexry,et4h%yluerneea,mo1n.o2oc8t%ySluphteonnyelfeotahmere,r, 0w.a0s6a%dsdoeddiautmalanuormylinsaullfactoen,ceanntdra0t.i0on5o%fto2l0yt0rimagz/olLe.. Antifoamer SWS 214. cThhaerafcotlelrowiiznegdscounmcmeantrryataipoplnisoesftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical component of the test sample. METHOD Method: OECD 209 TGeLsPt:tyNpoe: Static acute Year Completed: 1990 Analytical monitoring: Dissolved oxygen concentrations, pH and tSetmatpiesrtaitcuarle.methods: Resuits were determined by calculationofthe % iTnehsibtiotirognainnirsesmpisroautriocne:ratAec.tivated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN Test condition: DSiylnutthieotniwcasteewr:agMei:llippeorreOMEiClDi-mQTMetwhaotder Rreeffeerreenncceesuabnsdtatnecset,s3o,l5u-tdiiocnhlporreoppahreanotli,onw:asA sptroepcakrseodlbuytidoinossfotlhveing w5a0t0erm,gthienn1b0romuLgh1tNtNoatOhHe,podiinltutoefditnoci3pi0emntLpwrietchipMiitlaltiipoonrweiMtihl1lN-QHTMzSOx awnads dmieluatseudrteod1tLowbieth7.M2i.llT-eQsTMt wsautbesrt.anTcheewpaHsoafdtdheedrdeifreecrtelnycteostohluetion test vessels. Antifoamer SWS 214 was added to the test vessles at 200 214 mg/L from in 500 mL a stock solution Mill-QTM water. pTrheepasroleudtiboynsdialuptpiengar2e0d00mimsgcibolfeSinWS ~~~ water. Test vessels: Not given. Nbluamnbkecorntorfoclsoncentrations: three plus 1 reference substance and 2 Temperature: 18-19 C 001774 Total suspended solids and pH on day of testing: 7.853 g/L (TSS), initial pH 6.7 and final pH 6.9. Initial TSS in 1000 mg/L concentration was 3.16 g/L, initial pH 6.7, final pH 6.8, and MLSS 3.38. Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20 C for calculations. RESULTS Nominal concentrations: 2 Blank controls, reference substance, 320, 560 and 1000 mg/L Element values: 30-minute ECso = >1000 mg/L 3-hour ECs = >1000 mg/L Element values based on nominal concentrations. Remarks: There was minimal foaming in the 1000 mg/L exposure concentration when compared to FC-206CE without antifoamer. Testing was conducted on a mixture as described intheTest Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. ONCLUSIONS The FC-206CE 3-hour EC for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and thestudy lacks analytical confirmation of the amountoffluorochemical proportion in the solution. REFERENCES This study was conducted bythe 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number H1480-4, 1990. ~ OTHER Last changed: 6/26/00 001775 . ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR RHIN~E Date3/25/90 snalye TTS Type Teor Actiated Sludge "Respiration \abibition Test CECDE2A SourceMein \alaste ToeatmenttPant, STPaul, My Date Obtatned____xjzz Jan Date Used__x/2xa0 Mixed Liquor Suspended Solids (MLSS) "ASA g/L Adjustment gL PH initial to, final od Comments, = Test Substance - Sapmplee DDeesacrieptioA n EE Sauple PH_jgitial 1 Linal Lf aitRy nAaifarerSais21s (10mal) Adjustaent CommentsMLSSof loci,test.o0in; luitialSlag Lunal X28alls Reference Substance Sample DescWreipstti.on i 6 Sample PHintal 1b, Fiual 1.0 Comente_ Sick solution pH 12 Sowa Adjustaent, Exposure Conditions TestDuration Aus Test Temperature_\P-19 c - Comenta --_-- 001776 Sample Description Environmental Lab -- Work Sheet . . . RESPI(ROAETCIDOANCTIINVHAITBEIDTISONLUTDEGSET #209 Lab Request No. HI480-1 o"a2t0e: --13\/2--8/9of0= Analyst: 5 TestRl Conc. a 5.0 1000% nDg oadtdead rrr 2805 + 500 + amim0Ra l Hedsynm.cssewiaege mlTmmeicortoeblial + 200 254 +25 . + 200 28a +25 + \p + 200 Mivital Soar Total 5volume = soo = son = son 001777 Be=b ENVIRONMENTAL LABORATORY p83 203 OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAK DATA Test substancesHC 200aCE,Let.5022ude )unityAuntioaimer SWSLN(200ml) Source of Activated Sludge Mixed Liquor:MetroWasteTeatment Plat,SEBul,Ma Date Received: 5/25/40 Date Used: _XJ23/an Analyst: Fuchelle Fihideau-- 30 - MINUTE EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS ng loafasfasasaaalaaaafilo| CONTROL I 20 oo ooo ol0 io 8 lra1lo1a| tpl2e 1dep lalesl I LT1 hl8a la Pre rrET te tert SEE ro 1 [oo [los alae a[2s va lusloa PBs 3 - HOUR EXPOSURE est Substance DISSOLVED OXYGENRCIONNUCTEEN:TRATIONS mg TH Ee CONTROL I 0 100 e2Silloealp e{uaelru2 lzPoEhu| 0al|ooa ha ls Foea arbseeillle[ess|lmouza| lraT ois sT[nla lialo EN sown. 1 [alualualzalzairlizzllozsal llie pTTiHesootffTAehcmitpgiehvreaasttteudrceoS:nlcue1dn8gt-er1aM3tiixoCendtLeisqtueod:r: IInniittiiaall LLJN] FFiinnaall 1L0A8 001778 ENVIRONMENTAL LABORATORY RHINE Test Substance EL A2C0T6IV0A6TLED kSLUSD0GE2CRA2ELSCPaUIlLReAmAT)TIwIOONiNAtOhFINARHrEISBkUIiL"TfTIoSOaNrTmEeScTS#I20S9 214 He (200mg)ysPd3eST 30. MINITE EXPOSURE ConcTeensttraStuibosntan(cneg/L) CONTRO) Oxygen RUptake Rate tRoCo2r0gCected ) + RIensihipiibriattiioonniiRn at(e? [o| tamt e 0 0 ems | ass | | aue |o masg ---- Bay 5 000 [= 1 1 CONTRO) | er | zar | Percent Difference in Control(s) WR% oNS Bly (95% Confidence Limits)mb cell EXPOSE Test Substance Concentration (mg/L) Oxygen Uptake Rate R Corrected | Inhibition in p to 20C Contr | ama | asp | Respiration Rate? 0 la) | [ vwes s [ TTz i eaa n | | a p -- p<--s 7foh&o. 000 | woaio | Luar | coNTRg | owt | Percent Difference in Controls )___54%" Ee (95% Confidence Limits) ee 0) Tewp. Correctionz --DRL o RC, + RC @ Innibition=. ot (ng 0,/L-min.) x 100 eg | | 5% ________ (9) Difference =i--L_--dx 109 RC, + RC} { 7 ). Tshheoultwdobceownittrhoilnr1e5s%piorfateiaocnhroattehse ThTe,or=besO:xy=geSanmupptlakee"termapts.C(n,gz O/2L-nin,) 8C'==1C.o0n4t7r,ol "x = T,obs - 20C *Rate vetcal5 c oxygu en dl eplea tedbtoolrape iidldy ly; yiyinnllessss, : tan Limindee.ne '! ENVIROWMENTAL LABORATORY Darteoljzuz/as0s e ACTIVATED SLUDGE MIXED LIQUOR TyeeTestAckvatedSludgeResgostion_ldinition Test OFCDE2N sourceU lMelnfastPlT ant,e SATa PAOt Mawon Dace Oheataed___=j250 Dace Used___x Jz:Jan PLLal0 Mixed Liquor Eid ea Suspended Solids (MLSS) 1.85% g/L Adjustment g/L Cometments--.s-- em -- - e lee Test Substance Semple DeVsiegrtnpetsion isZuolRAukifonu)o`r c(4o1olegiie)SlicesCregtration Adrian' Sample PR itial Lo] final (0. Adjustment Comments Sherk slicer pla] Reference Substance Sasple Description Wight . rested, 10ngafC Sample PH Ral Lb, Flual M.D `Adjustment Comente_Zptous splution 3H 1.2 `Exposure Conditions Test Duration Yours ~ comentSs, E Teast Temperature |@- 9 c NS: O -_ 00 -_ : 0m0;01780 i \mple Description STOCK SITIO: Lab Request NoW.140 =, Environmental Lab -- Work Sheet RESPIOREATCIDONACTIINVHIABTIETDIOSNLUTDEGEST #209 0 --l-- of 3 bate: 2/25/90 =D Stock and Test Solutions Preparation 2000/05 0005 Fath Lrls =Vig _-- --_e --------eeee srrrreietereeeleeeeeeemeesemm--eeseeees SSBTgl, Conc, pWl eStocrk msoln pemrali$0va0tmer nfleesdypn.orse5v0ag0em WilnoWcuGluRmbpeTra5l00m TotalnvloTme 000 25 + Isa + i + 200 = 500 _-- --- -_-- --_---- _---- --_------ e e e ----a ---- -------- ------a -- see -_-- p--stm----------------------------------=--= ---- pp ------ -_-- a. -_-- 0017S1 : ENVIROMMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 " PEe2eTS RAK DATA Test substance:SIS214 Anti@amer(411leie) Source of Activated Sludge Mixed Uauor:Met Silicones(hopooation,Adeiag, Mj, laste TrghueaPak.SptFadMa Date Received: =/22/q0 AnalyseCode EFabien Date Used: =fzxfan~~ 30. - MINUTE EXPOSURE entration (og Concentration (mg/L) = [00 CONTROL 11 DISSOLVED OXYGEN CONCENTRATIONS gg 055Too Jos Joo0 los |cG RRR T 01.9 5r 10.8I 0r 10 rT r & Se EREEEH ===ss 8 NF q a sls 3 - HOUR EXPOSURE ConcoESnTtratSiubosnta(ncaeg) i DISSOLVED OXYGEH CONCENTRATIONS mo WE-HIN TH ERR 000 0l2 LT a% TT TTT 1 q T C T T TT T HHH como 11 [selualuglss azarae mlizlos] [ig TPPHHestooffTehAmciptgeihrveaastttuerdceo:Snlc1ue8dn~gter1a9Mtii0xo_endteLsitqeudo:r:. Initial (oN Initial |p. Final LQ Final lo8. 001782 ENVIRONMENTAL LABORATORY TestSubstance+3SAC2TI4VAATEnDkiSLfUoDnGcECnAReLEcCSUP(LIARuTAITaOINOlNO)FISNRHiEIlSBiUIcLToTInSON TEST #209 Cocpanesion, R_HI40-5 p7a3tedx5/rzaa/o0 AdviagVG 30 - MINUTE EXPOSURE ConcTeensttrSautbisotnanc(eng/L CONTRO] noo Oxygeonen opUptake Rate 5R0Co2r0geCcted(1) + RIenshpi+ ibriatti+ ioonniRnate(2 | omal | toma 1 Jo0% | pap | . CONTRO |ie | r may | PEegrocen(t95%DiCfofnefriednecneceinLiCmointst)rmogl/slTM--)___y go 00 Sl EXPOSE ConTceesnttrSautbisotnan(cneg/L) CONTRO |OcygenaUspRtaake Rate | Rt0Ceo2r0gewcCted!(1) | * RIenshpi+ ibriatti1 ioonniRnate(? oon | usse | gta | ---- CONTROL [wo 1w ams| oPegrgcenOtSDiCfofnefriednecneceintCimeos)nm/tl rot5s%)___ : (1) Temp. Correction= o* (ng 0,/L-min.) Re, + RC. 4x (2) % Inhibition: X 100 [fev=) hTe,or=besO:xy=geSnamupplteakteermapte,C (ng2 O/2L-nin.) 8:Lowry x = T,obs - 20C - trol (3) Difference=--1 "dx 109 RC, + RC} The two control respiration rates should bewithin154 of each othe : 0017-8. So aMEnveonrmpe,ntalLaSboolriadtso[ryIS~]R[a1w55D]a[tTOSSh]eet --a] <3 een MethodRafrsnc:St.Metros " nmcosBLias elTd S re E JER Sor AToRATRPe AStPPTaTul)Ma F = =g ooit[pe | woe|G | VEG| | mew| oe | T --{=e Tumilere Los Faoerer TT 1 ree 1Tom]