Document 995rQwrdegkRXNwrgNoL29goD

AR226-2821 1 Copies co: E I* du Pont de Nemours and Company Haskell Laboratory lor Toxicology and Industrial Medicine TEN-DOSE SUBACUTE ORAL TEST (RATS) Procedure : The test material (H-3824) was administered dally by stomach tube to each of six young adult ChR-CD male rats, five times a week for two weeks. Groups of three test rats, along with controls, were sacrificed four hours and 14 days after the last treatment. Results: Aqueous Susp,, % Dose (mg/kg/dav) Mortalitv Toxic Signs Individual Liver Weights (g) 4 Hours After 14 Days After 10th 1Dose 10th Dose Control T e a t Control Test Individual Liver Wt/ _________ Body Wt X 100 4 Hours After 14 Days After 10th 'ose 10th Dose Contro. Test Control Test 5 220 0/6 Slight depression in rate 17.6 15.5 19.7 16.8 5.1 5.0 of weight gain during test and recovery periods 16.7 15.3 17.0 16.0 5.0 4.9 16.1 18.8 16.3 17.8 4.8 5.7 4.7 4.7 4.5 4,5 4.2 4.8 0 . 5 22 0/5** Very slight depression in rate of weight gain during test period 17; 6 16.7 16.1 14.7 14.5 - 19.7 17.0 16.3 16.0 16.3 18.4 5.1 4.6 5.0 4.4 4.8 - 4.7 4.1 4.5 4.2 4.2 4.6 ** ^ animal accidentally killed with 8tf^se ACUTE ORAL TEST (RABBITS) Procedure: The test material (H-38-24) was administered as 33c.3% aqueous suspension by int-ragastric Intubation to male albino rabbits in single doses. Survivors were killed j.0-16 days later. Results: Dose feB/kg) 5000 3400 2250 1500 Mortality 0/1 0/1 0/1 0/1 Toxic Signs Weight losses for 12 and 8 days, respectively, at 5000 and 3400 mg/kg; decreased food and water consumption at these doses; urine of all rabbits, first 24 hours, clear, foamy and brown Weight When Killed Body (g) Liver (g) 2189 135 2486 110 3137 170 3139 179 Liver Wt _____ Body wt 1 100 6.2 4.4 5.4 5.7 ALD > 5000 mg/kg .Company Sanitized. Does not contain TSCA CBI ACUTE SKIN ABSORPTION TEST (RABBITS) g.F9cedur^ >e test material (H-3825), as received, was applied in single doses to the finely clipped skin of male albino rabbits and rubbed in lightly with a glass rod. The animals were restrained in wooden stocks for 24 hours, after which the skin was washed with warm water. Survivors were killed 14 days later. Results : Dose*** (mg/ke) 3765 2250 1500 Mortalitv 0/1 0/1 0/1 Toxic Siens None Weight When Killed Body (g) Liver (g) 2869 122 2657 167 2734 88 Bodv Wt X 100 4.2 6.3 3.2 ALD > 3765 mg/kg TEN-DOSE SUBACUTE SKIN ABSORPTION TEST (RABBITS) Procedure: The test material (H-3825), as received, was rubbed Into the clipped skin of six male albino rabbits, five tim';3 a wee for two weeks. After each exposure, which lasted six hours, the material was washed from the skin with warm water, ree test and three control rabbits were sacrificed four hours and 14 days after the laqt treatment. Based on active ingredient. Results : ose**** 1500 Mortality 0/6 Toxic Siens None **** Based on active ingredient. Individual Liver Welehts fa) 4 Hours After 14 Days After 10th Dose 10th Dose control Test Control Test 131 103 86 105 82 110 124 180 108 140 139 157 Individual Liver Wt/Bodv Wt X 100 4 Hours After 14 Days After 10th Dose 10th Dose Control Test Control Test 4.6 4.2 3.1 4.4 3.6 4.6 4.2 5.9 4.3 5.2 4.8 5.4 I l o w 'umula*lve oral toxicity for the male rat When administered repeatedly at doses of f h?!e XgS^IflfliTM>trically and histologically within the range of normal. Th s should be contrasted with the results obtained w h e n W B R w a s administered orally to rats at a rate of 2259 tng/kg/day the S e r s of these animals were extremely heavy and histologically abnormalfTM*1*" ^ -- 7 tne llvers i v . . e iaa ^ow acute oral toxicity and low acute dermal toxicity for the male rabbit, the Approximate Lethal <" >> b? S * * ? > > " 5" "8/1 - a greater than 3765 - / k g , r e s p c t l . . l y ? t t ^ M Lc bodv rfbl When admJ" l8tared repeatedly via the dermal route at a dose of 1500 m g / b g / d ^ Liver weights and liver/ which could hp* ccnsidered normal for all the rabbits examined; moreover, the livers did not exhibit any aberrations which could be attributed to the administration of the test material. to liver wliohi1? 6" "*6 betweea th response of the rat and that of the rabbit to acute oral doses of-, ____________ ght suggests a species difference. Organs other than liver were not studied histologically Lth respect 2Report by:_ Approved by: HS/mfs Date: May 6. lQfiA