Document 93jbOQkeB9g46qndEXdRkyROL

AALLGGIICCIIDDAALL AACCTTIIVVIITTYY I TESTE SUBSS TANCT E SUBSTA0N 00G 00E 0 IIddeennttiittyy:: AArefmmeirixxrtteuudrreetoccaoonsnttPaaiFinnOiinSngg, ppFeeCrr-ff9ilu5uo,orrooorooccattsaannaeescsuuolffmfoopnnaoattnee,e, wwnohthfiiccFhhC-mm2a0a3yy.aalls(s1oo- bbee rOecftearnreedsutlofoansicPaFcOidS), (FCCA-S95#, o27r9a5s-3a8-c3o)m. ponent of FC-203. (1- Octanesulfonic acid) (CAS # 2795-39-3). iRRseeFmmCa-ar2rk0ks3s:.: CuTTrhhreeen3t3MMinfpporroromddauutccittoiiononninllodotitcnnauutemmsbbeiterirswwaaassminnxoottutrnneoototeefdd1...T3T4hh%ee tteesstt ssaammppllee PFOS, 35% ddSisiiueeFltthCthyoy-l2lne0ennf3eeo. aggmClleyyurccro,orlel3nbb%ututtiynsyllofoeedrttimhhueeamrrt,,ioo33cnt77y..iln88d5s5ui%c%lafawwtetaaest,teeir0tr,,.is122a%00%%msoieexdtttihuhuryyemlleeonlnafeeu!rggy.l3llyy4scc%uoolllf,,Pa22tF.e.6O,666aSn%%, d35% 00S..u00l5t6o%%nettooflloyyalttmrriiaeazzro,oll3e.%. sodium octyl sulfate, 0.1% sodium lauryl sulfate, and cTThhhaeeraffocoltllelorowwiiiznneggd sscouunmmcmemnaatrrryyataaippoppnllsiioeessfittmoopaaurmmiitixxitetuusrr.ee Dwwaiittthha iimnnaccyoommnpopltleetatecelclyyurately rrceohffalieercacttcttteoorxxiiizcceiidttyyocofotfnthcheeenfftlrluauotoiroroonccshheeommfiiimccaaplluccrooitmmieppso.onnDeenantttaoofmftathhyeetnteoeststat cssacamumprplaletee..ly M0 MeEmTHoOnD0 :: 0000000 CMMheeettmhhioocdda::lsSSattanadnn/ddoaarrrddPrNNoooc..e22s22s,e, sRRe(elNlaaatttiiinnoggnattolo SSwwiimmmmiinngg Sanitation PPooooll WWaatteerr Foundation) TTrreeaattmmeenntt TCTGLyyhpPpe:eem((ictNteaeosslstt attyynppdeet)o)::r PrAAocccuuetsteeseSSsttaa(ttNiiccational Sanitation Foundation) YGSYpeeLeaaPcrr:issettNsuu:oddyy ppeeCrhriffooorrrommlleeadd:p:yre11n99o77i4d4osa and Phormidium inundatum SSSInopocu.eu,rcrcNcieeoe:rs:t:hIIbnnr--ohhooCokuuh,ss/eoeILcr.ecuulllatltuuprryeerssenmmoaaiidinnottsaaaiinnaeendddaatPt IIhnnoddruumsstitrdriiiaaullmBBiiiono-u-ttneedssttatLLuaambboorraattoorriieess,, EEtfnrllcbee.im,mdNeiemnonetrttthrbbibaacsrsoimiosse:k:a,sIuLr.eCCmeeellnl tpp,ooppuullaattiioonnss ccaallccuullaatteedd ffrroomm hheemmooccyyttoommeetteerr ccoouunnttss,, tESEutxxrapbptioiodsssitmuuirceraeetlrippcMeeemrrtiieohoaodds:d:usr:e1144mNddeoaantyyt.gssiven. ASAntnaaaltiylsytttiiicccaaalll mMmoeontinhtiotoodrrisinn:gg:N: oNNt ogottivggeiinvve.enn.. TToesstt CCoonnddiittiioonnss:: AAllggaall NNuuttrriieenntt MMeeddiiuumm:: AAlllleenn''ss nnuuttrriieenntt mmeeddiiuumm.. SaSlttgooaccekkwaaintnhdd algae with 1tt0ee0ssttmssLooollufuttiAiololnnenpp'rrseepmpaeardraaittuiioomnn:u:siAAnllgggaaall 100 mL of Allen's medium using a WWssaooalrluudittnniioggonnBBsslleppenrnrededepperaar.r.reeOOddnnbbeeyy mmbbllLLeennddiinngg aadillisiqtquiulooettdss distilled wooafftttehhree water attenessdtt and assduudbbsestdtaan1n0cceeAlwwleeenrr'ees added to Allen's hhmaaendndidluleemdd. medium. aassOOnn11ee0000mm%%LL aaooccffttiaiavvddeejjuuaassnnttddeeddddiiccleuuelttlleledd wwiitthh ssuussppeennssiioonn wwaass aaddddeedd ttoo eeaacchh tteesstt ssoolluuttiioonn,, ffoorara ffiinnaall vvoolluummee ooff 110000 mmiL.. ENExuxppmoobsseuurrreeofvvreeesspssleeilclssa::tesFF:llaass1kkss ccoonnttaaiinniinngg ffiinnaall vvoolluummee ooff 110000 mmLL.. NIIPnnhuiiottmiiraamlblieccdoreilullolmflloroaeapddiliinncgga::te77s..5:5 xx1 1100`= cceellllsslmimL. ffoorr CGhhl/oorreellliea,, 1122..55xx 1100`= cceellllss/m/mLL ffoorr Phormidium . Exhibit 2714 22771144..00000011 SM_MNO043645 3M_MN00436466 blank control for Phorum. NN umu beroom ffccoonb nccenetne rtarattiir oonnss:: ssiixx pplluuss aa bbllaannkk ccoonnttrrooll ffoorr CChhlolorreellllaa,, ffiivvee pplluuss aa blank control for Phormidium. incubation, with fina readings mado aftr 14 days Inoculation. Thies ditions LLiigghhttiinngg:: NNoott ggiivveenn SSuubbccuultltuurree:: IInnooccuullaatteedd bboottttlleess ssuubbccuullttuurreeddttooffrreesshh mmeeddiiuumm aafftteerr oonnee ddaayy incubation, with final readings made after 14 days inoculation. Three dilutions oofftthhee tteessttssuubbssttaanncceewweerree ssuubbccuullttuurreedd ((11::55,, 11::1100,, aanndd 11::1100). W Waatteerr CChheemmisitsrtryy:: NNoott ggiivveenn.. Bs ESTS ence NN1:oo1mm0i0inn0aa0lldccioonnoccenenntftrorraatCtihioiobnnrsse::llaBBKkpyccrooenntntrorooklli,,o11s::a55,,st11u::d14y00.,, 11B::K1100c00o,,nt11r::o11l55,001,,:511,::11100:001000,,, aa1-nn1dd00, 1:10000 dilution for Ch/ore/la pyrenoidosa study. Bk control, 1:5, 1:10, 1:100, 11::11000000,, aanndd 11::1100000000 ddiilluuttiioonn ffoorr PPhhoorriinniiddiiuumm iinnuunnddaattuurnm ssttuuddyy.. Resultsof the Study with Chlorella pyrenoidosa Results of the Study with Chlorella pyrenoidosa SSaammppllee GGrross AAppppeeaarrance TTuurrbbiiddiity ((ddililuuttiioonn)) 7 ms 1 --n=oe-- [-- 00Cc--1 || Control ((CCoolloorr)) green ((aabbssoorrbbaannccee)) >2100 [ [i we o 11:1:50 [ [m m nnoonnee o o || n =we<<00e oo.,0011e rr || [[faffoso1:1000 | |SSglgigghretnegoeernenenenttnintt ||____0<2O00.0,O03310 || 5 T1:010~)0000 1s:ulobooOul0ire | | genggrroeeeneennne || <2>>0220..0000010 || 1T11:0i1:5O00sssusuububbcbccucuullutltuttuuurrrereee || nnonnooonnnneeee || __<<<<000...0000111 01|| . 1 :! O0 subculture none <0.01 RReessuullttssoofftthhee SSttuuddyy wwiitthh PPhhoorriinniiddiiuumm iinnuunnddaattuumm SSaammppllee GGrroossss AAppppeeaarraannccee TTuurrbbiiddiittyy (d(dililuuttiioonn)) ((CColoolorr) ((aabbssoorrbbaannccee)) 7I ------ Control [15 now wor] i:5 green none >2.00 <0.01 SFI --CS---- -- [_T101:1:10000 Tnnoon nnee |e <0<00.101T | [mom goentmr [200| "t :1000 green tint >2.00 I ---- -- 1 : 10000 green tint >2.00 [ASsuboutture| none | _<001 | 1:5 subculture none <0.01 [7:10subculture| none |--<o0r 1 1:10 subculture none <0.01 [2100subcuture| none |--__<o0r J 1:1 O0 subculture none <0.01 EElleemmeenntt vvaalluueess aarree bbaasseedd oonn ddiilluuttioionnss ooff nnoommiinnaall ccoonncceenntrtraattiioonnss.. 22771144..00000022 3MM_M_MNONDOA034386446677 RSRueebmmsatararkknssc::e TTReeessmttiiannrggkwwsaafssielccdoo.nnTdduhucecttveeaddloouennstthhreeepmmoirixxttetuudrreaepaapsslydtdeeosstcchrraiibtbeemddixiintnuttrhheeeaTTneedssttnot ttShhueebffsilutuaoonrrococechhReememimiccaaalrlkppsrrfooieppolodrd.tiioTonnheaallvoonaneleu..es reported apply to that mixture and not cCoOnNoClLuUsSiIOoNnSs AAllggiicciiddaall rraatthheerr tthhaann aallggiissttaattiicc aaccttiioonn ooff tthhee tteesstt ssuubbssttaannccee wwaass oobbttaaiinneedd.. SSPuauubblm,mitMitittenernr:e:sot33aM, CC5o5om1mp3pa3annyy,, EEnnvviirroonnmmeennttaall LLaabboorraattoorryy,, PP..OO.. BBooxx 3333333311,, SStt.. Paul, Minnesota, 55133 RDAATTAAQQUUAAILTLTYY (eee eee. RmReeeltiliaahbboilidltityfy:o:llKKoiwliiemmdii.ssccThhhrreaannskkaiimnnpggl==e 33p..uriSStyttuuwddayysllanacocktkssprddoeepsseccrrrliiyppttciiohonanraaancntdderrreiezcceoodrrddassnoodffttthhheee mssttueudtdhyyolldaacfcokkslsloaawnnaealdlyy.ttiicTcaahlleccosonanfmfiirrpmmleaattpiioounndtooyff wtthhaees aanmmootoupunrntotpooeffdffylluuocohrroaocrchahecetmmeiriccizaaell dpprraoonppdoortrthtiieoonn iinn tthhee ssoolluuttiioonn.. RE~ RFEFEERREENNCCEESS ~ TTILeeas~tt twwhaaessrcceooqnundedsuutco~tfeedtdhbbeyy3lInnMdduuCsstor~mipaalalBnBiyieo,T-TSete.ssttPLaLauablb,oormMaNt~o,rdi1ee9ss7,,4.Ilnncc..,,ooffNNoorrtthhbbrrooookk,, IL ~the mque~ofthe3M Company,~.Paul, MN, 1974. COTU HERR ---- LLaasstt cchhaannggeedd:: 662/277//0000 22771144..00000033 IM_NNOO436468 3M_MN00436468 ly 5 Qe one Zaza 1B8I1O0 F-RTOENSTATGELaRbOoAraDtories, Inc. NorY1u8e|0rFoRoO kN ,TIAtGLEiNoRIOsAsD00s2 HBROOK, ILLINOIS 60069 ==) RREEPPOORRTT TTOO 33,M~ CcOoMm.pPaAnNyY TWO SAAALLM(GP]ILICCEIISDDAALLLAAABCCETTLIIEVVDIITT"YYA"OOFAFND "'B" ,T~VO SAIV~PLES LABET.~.D 2"A'" AND 23 JJAANNUUAARRYY 2Z33,, 11997744 IIBBTT NNOO.. 663333-0044447733 ) : ) 22771144..00000044 SM_MNOD43646 3M_MNO0436489 Snitch BIO TEST Laboratonss, Ine. . 3 REPORT TO 3M COMPANY TWO SAALMGPILCEISDALLAABCETLIEVDIT"YA"OFAND "B" . JANUARY 23, 1974 JANUARY 23, 1974 IBT NO. 633-0473 LBT NO. 633-04473 I Introduction : I. Introduction At the request of 3M Company, tests were conducted to determine At the request o5 3M Company, tests were conducted to determine ) tthhee aallggiiccilddaall aaccttiivviitt~y oaf ttwwoo ssaarmr~pplleess identified identified as ag "A" "A" and and "B". "B'. Two Two species of algae, Chlorelle pyrenoidosa and Phormidium inundatom, species of algae, Chlorell~ pyrenoidosa and Phormidium ~n~ndatum, were used in the eveluation. v were used in the eveluation. ~ ..J 22771144..00000055 SM_MNO0436470 3M_MNO0436470 Dulustiol BIO VEST Loboabsn,I 2 .2 3 Io. Summary Two Two samples, samples, identified ideut~ied aass ""AA"" aa~ndd ""BB"',, w weerree ssuubbmmiitttteedd oonn A -- November 2200,, I19~ 73,, bbyy ~3MM C Coommpapnany.y. ~ Thee asl~giiccii~daall tteesstt wwaass ! initiated on November 30, ~nitiated on Hovember 30, 11997733,, u~ssiinngg ttwwoo ssppeecciiee~s ooff aallggaaee,. C Chhlloorreellll~a Brencidoss Wise. pyrenoidosa ~isc. 2005, 2005, Phormidiam Phor~idi~ iinnuunnddaattu~m WWisisee.. I100~953.. The The test test was was conducted conducted according according ttoo a a mmeetthhoodd ~outtllinineedd aanndd Prepared _pre~red by the National Sanitation Foundation, by ~e Na~o~l S~n~tation Foundat{on, SSttaannddaarrdd N Noo.. 2222,, Relating Relating to to Swimming S~ing Pool Pool W Waatteerr TTrreei~ tmeenntt CChheemmicicaallss aanndd//oorr Processes. ~rocesses. The ~e ~torbrb~iddi~meettrriicc ddeetteer~mii~nat{tioonn ccoon~foorrmmeedd ttoo tthhee pprroo-- cedure recommended by Fitzgerald. * cedure, reco~ended ~ Fitzgerald, AA 1I~:1I0000 ddi~lluuttiioonno~f SSaammppllee ""AA"" oorr ""D B"",, eeffffeeccttiivveellyy coonnttrroolllleedd tthhee ) proliferation of Chlorella sp. and Phormidiam sp. for a 14-day period. proliferation o~ ~lorell~ ~p. and ~horm~di~ sp. for a 14-day period. Cid rather than static action of the candidate algacides was obtained. ~idal rather ~n static acti~ o~ the candidate hlgacides was obtained. *~ FFiittzzggeerraalldd,, GG.. ~P.. B Biiooaassssaayy ffoorr A Allg~iicciidda~l vveerrssuuss A Allg~isisttaatti~c ~ Cheem milccaalsls,, `WWaatteerr ~ andd SSeew waaggee W Woorrkks~,, 111100--2299~6 ((1199~633)) ~'~" ~ Respectlally submitted, Re s pe~ully s~b~itted, ~INDDUUSSTTRI~ALLBBIOIO--TTE~STT ~LABBOO~RATTOORRI~ESS,, ~INCC.. "3 Report prepared by: _DanDielrChane, M.CSl. an DGarnpiaep~ LCehaadn,erM.S. Janoary 23, 1974 ASAe..ctD DiaoavnviidHdeBBaadas,~kkiMinni,,ckrP~ohhb..iDDo.l. ogy Section Head, ~icrobiology . aJanuary Z3, 1974 dp J 22771144..00000066 awnoose 3M_MNO0436471 Sadish BIO-TEST LoboroniJauss * 3 3 mL. Procedure !If. Procedure A. PreparstionofInoculum A. Preparation of Inocu]am k TTw woo ssppeecciieess ooff aallggaaee,, CChhlloorreellllaa ~p.y~rrerenncoiiddoossaa W Wiissce.. 2005, and Phormidiom inundstum Wise. 1093, were used as the test and ~ormid~um in~ndat~ Wisc. ~0~3, were ~sed organtems. Each organism was subcultured at weekly intervals fn organisms. E~ch org~ism was subcultured ~t weekly intervals Allen's neutrient medium for three weeks, prior to inception of the test. Alien's neutrient medium for t~ee weeks~ prior to inception of the test. The suspensions of Chlorella and Phormidium were adjusted ~e s~spensi~s of C~orella and ~orm~di~ ~ere ~djusted ttoo ccoonnttaatinn 77..55 xx 11006 aanadd 11Z2.. 55 xx 11006 cceellllss//mmll rreessp~eccttiivveellyy.. ~ Thee~y w weerree prepared by shaking the algae growth in the flasks, to which was added pre~red by shaking the algae gro~h ~ ~e flasks, to whi~ was ~dded 100 mi of Allen's mediam and blendertzed for one minute fn a Waring . 100 ml of Allen~s ~edi~ and blenderized for one ~inute tn a ~ar{ng Blender. Cell populations were calcalated from hemocytometer counts. Blender. Cell populations were calculated fro~ hemocytometer counts. 3 B~.. PPrreeppaarraattiioonn ooff SSaammppllee One mi aliquots of test samples handled as 100% active were ~e ml al~qaots of test s~ples handled as 100% active were diluted in distilled water and added to Allen's medium for a final . diluted in distilled wa~r and added to Al~en~s medi~ for a f~n~l concentration ranging from 1:5 to 1:10,000. These concentrations 3 ~oncentrafion rang~g fr~ l:S to l:10,000. ~ese concentrations were achieved ater the addition of 1 mi of the adjusted algae cell were achieved ~ter the ~dition of 1 ~ of the adjusted algae ceil "uspension for a final volumeof100 ml. The numbers of Chlorella suspension for a ~inal volume ~" 100 ml. ~e n~mbers of ~lorel]a aanndd P Phhoorrm~iddiiuumm cceellllss uusseedd wweerree 77..55 xx i100 4 aanndd 1Z2.. 5 x 10%4 rreessppeeccttivveellyy.. Six control flasks, two per species, were also prepared. All S~ control flasks~ two per species, were also prepared. All inoculated bottles were subcultured to fresh medium after one day inoculated bottles were s~bcult=red to fresh medium ~ter one d~y incubation. Final readings for the presence of algal growth in all incubation. Final readings or the presence of algal gro~h in all bottles were made fourteen days after inoculation. bo~tles were made fourteen days ~ter inoc=lation. J 22771144..00000077 aM_oos3es72 3M_MNO0436472 Jeduirial BIO -TEST Laboralovies, Ine. + 9 IIVV.. RReessuullttss ? | TThhee rreessuullttss oofftthhee g~rroowwtthh eexxaammiinnaattiioonn aanndd tt~urrbbiiddiirmneettrriicc m meeaassuurree-- m meenntt ooff aallll ffllaasskkss itnnooccuallaatteedd w wiitthh CChhlloorreellll~a ppyyrreenncoiiddoossaa aanndd PPhhoorrrmniiddiimamu inundatum used for the algicldal tests are presented in Table 1 and lnundaturn used for the algtldal tests are presented in ~'able I and I respectively. respectively. ) J 22771144..00000088 SM_MN0436473 3M_MNO0436473 Suduibic) BIO TEST Loboroioras, Ine 5 y TTAABBLLEE I RReessuullttss ooff tthhee GGrroossss and and Tarbidimetric Turbidimetric ]E~xwamam iniantati[oonn ooff CChhlolorreellllaappy~ryernencoi[ddoos~sa. : Samdpilluetio"nA)" A Allggiicciiddaall TTeesstt G~r-roosesscoAAlpoprpp)eeaarra~nnccee | (color) .- abTTsuuorrrbbbiaiddnicitteyy) (absorbanee) 111:::5510 l11a:::l1sO 100o00 -11::115,0000 _Loh11::15Io,0s0,ou00b00oc0ulture 111::::1511000s0ssuuubsbbucccbuuuctlutltutluurtrreueere Control 1:!00 subculture C ont r ol nnmooonnneee nnnsooo lninngeeeht green tint gslriegehnt green tint ggrgnorreneeeeee.nns zlone nnoonnee. l~tone- ,. " ngoreneen <<<oo0...000111 <<<.00o0.,_.00.0o113l >2.00 O. 03 > 2.00 _22<>o2...0o0010 <<<<o000....00001111 >2.00 <o. oi > 2. oo Sample "8" 111:5:150 nnoonnee <<<000...000111 1hh11l1O 1050000 n"loingeht green tint <<<0o00....00001311 . 1:150 slight green tint 0.03 1:11:1:1l,0,,000000000 gggrrreeeeeennn >>>22Z.., 00O000 `7 5 1111::::551100ss,uu0sb0bucb0cuculultltuutrureere ngnnooornenneeee n ><<<000Z....000O1110 h1:11000susubbccuulltutarere Control l : lO0 subculture none none gnroenee n . <<<S0002...00001101 Control green > 2.00 , Ce] Deg To, "og, #3 SM_MNODS36474 3M_MNO0436474 22771144..00000099 ------------ Sitainsl BAO TEST. Loboriors, Ine 6 D ` ) TABLE I TABLE H R R`eeEssxunalmlttissnaoot~fittohhnee;o:GfG~rPoohseossriaan~nidddTiTauusmrbbniidudipimm deaetttrruiicmc Examination o~ l~horinidiurn inundation Algicidal Test : Algicidal Test Sample "AY dilution) Gross Appearance calor) Gross Appearance (color) Turbidity (ATbusrobribdaintcye) (Absorbance) 11:510 ~11.:11000 11.411,00000 -~l1::00l5,..0s00u00b000c0ulture 1111::::5111000s0ssuuusbbbuccbcuaculluttllutautrrureee,re Central l ; 100 subculture Control nnnooonnneee nnoonnee. n groeneentint `nome green tint green tint ~_reen tint nnonnonoonenne.ee green nolle green <<<oo000...000111 <o0..0011. <>02..0010 Z. 00 --<TI2o.00or <<<0oo....00001111 <>02..0010 2.00 Sample "" Sample 1I1::5S10 1111::11111000,00000 1111:::,15i10,0,os00u0Ob00Oc0bulture 1I11::1: S1I1000s0ussubsuubbcbcucucullutlutlturuterureere Control 1 : 100 sabculture C ontr ol nonnnooenn.ee non ngnoorenen.eeen tint nogggrnrreeeeeee.nnn tinttint tint nnnooonnneee ngroeneen green <<<o000...000111 <<<>0002....00001110 <5o2Z2....00001000 <<0000..o.,00001111 >20..0001 2.00 . . 35 22771144..00001100 SM_NNODS36475 3M_MNO0436475