Document 93daORgBXVryJ8v5GywwKJnnq

BACK TO MAIN BIODEGRADATION (Anaerobic Sludge) TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 217 (TN-A-2130). The test substance is a white powder. Purity determined to be 86.9% by LC/MS, 1H-HMR, 19F-NMR and elemental analyses techniques subsequent to analyses cited in the attached report. METHOD Method: Designed by Springborn Laboratories, Inc. Test Type: Anaerobic GLP: No Year completed: 2000 Contact time: 56-days Inoculum source: Anaerobic digestor at the Rockland, MD wastewater treatment plant. Inoculum loading in test vessels: 300 mL anaerobic sludge per liter medium Test medium: Mixture containing dried sludge extract (from rotating biological contacter wastewater treatment, Bridgewater, MA), unspecified OECD mineral media, and resazurin indicator. Media prepared under nitrogen p urge to exclude oxygen. Test concentration: ~20.8 mg/L (nominal) Study design: Inoculum control: inoculum, nutrient media Test: inoculum, nutrient media, PFOS stock solution Incubation conditions Temperature: 35C Lighting: Conducted in the dark Agitation: Not noted Test vessels: Twenty 160 mL serum bottles, containing 100 mL test solution, (purged with nitrogen after filling), with crimped butyl rubber tops. Dosing procedure: Each test flask received all components at test initiation. Sampling frequency: Days 7,14, 21, 28, 35, 42, 49, 56 - test bottle Days 7, 56 - inoculum control bottles BACK TO MAIN Analytical method: Aliquot removed from each bottle and centrifuged. Both the supernatant and the solid biomass portion of each aliquot were then analyzed via LC/MS. Remarks: Stock solution used to dose the biodegradation test systems was prepared at a concentration of 1,060 mg/L. This concentration is approximately twice the water solubility of PFOS RESULTS Results of PFOS analyses i ndicated no apparent PFOS biodegradation over the 56-day period. CONCLUSIONS PFOS is recalcitrant in the anaerobic system. DATA QUALITY Reliability: Klimisch ranking = 2. Study not conducted following Good Laboratory Practice Standards. The stock solution used to dose the systems was prepared at twice the water solubility. No Day 0 samples were taken to determine starting concentrations. REFERENCES This study was conducted at Springborn Laboratories, Inc., Wareham, Massachusetts at the request of the 3M Company. Report completed 10/31/00. Lab Project number E01-0434. OTHER Last changed: 6/12/01