Document 93aJKyQRQ12QL1y2DqGBEe225

AR226-04[3 estsvestance ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulfonamido) ethyl acrylate;mayalso be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafluorooctyh)sulfonyilaminolethyl ester, CAS # 423-825) Remarks: Material is an amber solid. Sample purity and not recorded. 3M lot number 13. METHOD Method: Not given Type: Static acute range-finder GLP: No Year completed: 1984 Species: Pimphales promelas Supplier: Brady, Neb. Adinsasloyltviecdalomxoyngietnorriencogr:deNdo.minal concentrations only. Exposure period: 96-hours `Statistical methods: LCs could not be calculated as toxicity at concentrations above the watersolubility. Test fish age: Not given Length and weight: Length: 3.79 cm Weight: 0.359 g Loading: 0.6 g/L Temperature, pH and there was insufficient Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry during thestudy: DO: 6.5-9.3mg/L pH: 7.982 Test temp. range: 21-22C Stock and test solution preparation: Test solutions prepared by mass addition of test substance to 3Lofdilution water. Concentrations dosing rate: Once Stability of the test chemical solutions: Not recorded Exposure vessels: Wide mouth one gallon glass jars Exposure volume:3 liters Number of replicates: one Numberoffish per replicate: five 000537 Numberof concentrations: Four plus a negative control RREESSOUWLTTSS 0000 Nominal loading concentrations: Bk control, 10, 100, 500, 1000 mg/L Element value: 96-hour LCso > 1000 mg/L. PeSrocemnatnM,ort|aliat es Pr [[FeCmome[| 0o|v v v v1o CA =Tot e == Remarks: All values reported are for nominal concentrations. No measurement of test concentrations was conducted. cCoOnNeCwLwUsSiIoONnSs The Et moll. FOSEA 96-hour LCs, for fathead minnow was determined to be > 1000 o0 DaATtAaQgUuA0 LaIrTYy 00000 Reliability: Klimisch ranking 2. This study, while conducted using the methodology acceptable at the time, lacks analytical data for determination of the test substance concentration in the test solutions and determinationofthe sample purity. Testing was also done well above the `solubility limit of the test substance. R0 REEFFEE0 RREENNCC0 EESS 0000000000 This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number B1228, 4/1/84 oOrTwHEeRr 00 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/22/00 000538 LL CHEMICALEENNVVIIRROONNMWEENNTTAALLELAFBFOERCATTSOREYVALUATION TTOXICITY TEST: SUMMARY REPORF . Chemical rested EY Sra. 2) 407 13 Test ee Rite Lier (@sdysesasicsosansy ox loa LL Geenren oan Jpn + MTTneeesslttyeDStap(teoec:)iCeso:m-E2A%7_UJEgATN SMingL)SYr(F)luesusr.lov:<FfaFompi2eg0wi8s 7 Exposure Type: (Exfosure/Test Conditions) (eraorn ng/L static_ |/Acre . Static Recirculatory, "I Diluents SOtrabtiitca/lConSthiakniunogus Static Renewal Flow-Through AWDeairtsaestroelvdHveaderdrOnxPeysagste:ne_r_%2,S(%aI/tnu)irtaitapiloomn.0: H.ar0dne.BSsms2e/C,1lsasspiHfGic,a7tio;n:TeAmp.LZD / ccti7pr)) Control(s) Lowest. Bxperinental bose 10gld Highest Experimental voselovowgll PH Levels: 48-hr 96-hr a 49 aS. Hp 7% Hs EExxptoesnudreed Control(s) Lowest Experinental Dose 10yalf Highest Brperinental bose tooowall Test Temperature C (F): 48-hr Bal al G2, 96-hr 8.0 M9 | EExxptoesnudreed 48h 96-hr EExxptoesnudreed Zhotoperiod: Sixteen hours light with a 15-30 minute transition period. - 00es053 BEST COPYAVAILABLE Jota EEITAL LOTR ve Te A eres SEXpPLrORyATt ORaYso(rRiAaNtGE-vFesItNeDdINEG XTAESLT)CR3U=2W 0)H2oe2s38Farysg Test OraanisnltmeanMupnlSource Fipder, Jz vateRec'dSJB Batch vo.554 Ave We 0,350 grams Avg. Size3 200M No. Organisms per dose 5 Test Tine SolutionVolume3Liters/All-Glass Tank Tank Started_2o/[ Spm Date Stared, S/25/5 Dimensions:WIDE-MOUTH | GALLON GLASS bate completed, "4/6 4 uAR DI(iFnluvlueltsitSoiigngant;Water Carbon-filtered Zwell waterso ((9T5e%mp.ConZf/idencec, Limi0t.s0) .2.23/00p0omm,alpnlaz zfemen [221 5 bly [0 lo ol Bilal ob *[ee [mTotmal ] Total Hours0.0] ES Added || No. Dead| pH | pp Totar 8 Hours 0.0.) oar IHour p.o. [[r9o6ter |Hour foo. No,Dead| pl pp No.Dead pi | ppm |INo.Dead bH [pom "100 3 CYR | [=00| ET | 2X7Bla4 n PTfPAN 2S | 0 kd 7 s |i ko L Tost Tom. c.r Laver I 2rer r ZT r T a . wis 1055/20GJ 5 20AT er3 a to fs Comores: IDIOMA _-- LEIGNTS..LIGHTAUECE Sor0 Jastceredl Effects other than Mortality eCSQ________ eealll A TAT - TreNm o. ||[ Symptoms or Reactions 24:Hr. 48 WrT.otal No, A7f2-fHerc,ted 96-Hr. FL 70 | EE wl 5. 0 OT | VY 3 g [os (J W Pw r IW IRS 3 [1 J --_-- mee em e--------------------------------------------