Document 91b0Q9bXyN58ZdOnjZ7OLNzJ5

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity: rPeefrefrlrueodrotoocatsanPeFsuOlSfAoAny,laFmCi-d1o(2e8thoyr)aascetthaetem;ajmoarycaolmspoobneent of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-|(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory sausbRs1t9a0n4c.e, C3u2rr%enwtatienrf,or1m4at%io2n-biuntdoixcayteetshaitnoisl,a 4mi%xteutrheanoofl,42an%dte8s%t Ca-C; fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. mTihxetufroleloofwtihngestuesmtmsaurbsytiasnacbebrinevaiawtaetedrb/eocragaunsiectshoelvteesnttssaolmuptlieoni.s a Data may not accurately relate toxicity of the test sample with that of thetest substance. METHOD: TMeestthotdyspe:: SOtEatCicDa2c0u9te GLP: Year CoNmopleted: 1997 Test organism source: Activated sludge collected from the City of Superior, Wisconsin, Wastewater Treatment Plant. RESULTS Nominal concentrations: Bk control, Reference substance, 3.75, 7.5, E15l.e0m,e3n0tmvga/lLu.es: 30-minute ECso = >1000 mg/L. 3-hour ECs = >1000 mg/L. (No inhibition exhibited) All element values based on nominal concentrations. dReesmcarrikbesd: iTnetshteintgeswtassubcsotnadnucceterdemoanrktshefimelidx.tuTreheofvtahleuetersetpsourtbesdtaanpcpleiaess to that mixture and not the test substance. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethyl)acetate. 1000411 _ I CONCLUSIONS The FC-129 3-hour ECso for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY. Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmationof test substance concentrations. There isa lack of characterizationof the test sample. REFERENCES This study was conducted at AScl Corporation of Duluth, Minnesota atthe request of the 3M Company. OTHER Last changed: 5/17/00 000412 ) EAnSvcilro"CnAoSmrceplnotSratalutdiTyoensI/tDAiFSnegl5D-0iD1vu0il-su0it6oh8n STUDY TITLE INHIBITION OF R1904 FOR ACTIVATED SLUDGERESPIRATION OECD GUIDELINE 209 JULY 10, 1997 AScI-Duluth EAnSvcilroCnomrepnotraaltiToenstingDivision 44 Airpark Boulevard `44 Duluth, MN 55811 Tel. No. (218) 722-4040 AScl Study TD# 5010-068 3M Company Lab Request? R1904 SSppoonnssoorr: St3uMdyCIoDmFpaRnIyS4 . 1 000413 EAnSveilrConomrepnotraaltToenst/iAnSgeDtiDvuilsuitonh "ASe Say IDF 5010068 1.0 GENERAL INFORMATION Study Title Inhibition of R1904 For Activated Sludge Respiration [s Daaundard| OECD Guideline 200. `Sponsor 23-M3EE-0n9v,ir9o3n5meBnutaslh TAevcehnnuoel,ogSty. and Safety Paul, MN Services, Building 55133; Tel No. (612) 778.7452. [| sponsor's Representative. Sponsor's Lab Request Testing Facility [[TrPrsoiinmcnisgppalFIaneveystirrgateor r |i DDeaftianiVtailviedaTteisotnDaantde Report SSeursvainceBse,acBuhi,ld3iMngE2n-v3iEr-o0n9m,en9t3a5l BTueschhnAovleonguye,anSdt.SaPfaeutly, MN 55133; Tel No. (612) 778-7452. ram] ADiSviIsiCoon,rp4o4r4at4ioAni/rApSacrkl-BDouulluetvharEdn,viDruolnumtehn,taMlNTeSstSi8n1g1; Tel. No. (218) 722-4040. Deion July 1, 1997 Alan Mozol Repos Sig pee P 4 P 21 | Retention Report of Raw Data and Final| Five years from the date study is completed. Location Report of Raw Data and Final | DAiSvcilsiCoon,rp4o4ra4t4ioAni/rApSacrkl-BDouulluetvharEdn,viDruolnumtehn,tal Testing | MN 55811; Tel. No. (218) 722-4040. J 2.0 OBJECTIVE RTIo9d0e4tefromriancetiivfaptoesdsisbllued,gethrees3p0i-rmatiinount.e and 3-h (contact times) ECsy's of the test substance | `GTuhiidsesltiundey2w0a9s(cOoEndCuDct1e9d93a)c.cording to the AScl study plan retained by the Sponsor and OECD SSppoonnssoorr:Stu3dMyICDoFmRpaIn9y08 . 2 000414 EAnvsieroCnomrenptoarlaiToen/iASnClD-iDvuilstohn "ASE Sudy IDs 3010-068 3.0 TEST METHODS { Test substance Properties: R1904, yellow liquid, specific gravity ca. 1.3 (water = 1), stable, and completely soluble in water. dTiersetctcoandcdeinttiroantsi.ons: 0 and 1,000 mg/L prepared in duplicate by I Reference Substance | Name: 3,5-dichlorophenol, purity 97%, Aldrich Chemicals, Lot/ | 02100. | "Test Organisms. Test concentrations: 3.75, 7.5, 15.0, and 30 mg/L prepared by direct addition of 500 mg/L stock solution in Millipore? water. Source: Activated sludge from the City of Superior (Wisconsin) Wastewater Treatment Plant receiving approximately 95% domestic | sewage. Use in test: 7500 rpm), (2()1)diCleuntterdiftuhgeedslfuidvgee mpielnluettesw,itthhrceaerbtoinm-etsre(a4teCd,water to | selected volume, final total solids concentration of 1.6 g/L was | obtained by further dilution, and (3) added SO ml of synthetic sewage | l fineietdiaptieorn.L of the diluted sludge, The sludge was held and crated for less than at 24 20C hours until test between collection and test initiation. Synthetic Sewage Feed | Composition: | Chemical g dissolved/L of deionized water { Deionized Water Bacto. peptone 16.0 Beef extract 1.0 Urea 3.0 NaCl 07 CMagCSlO,,2H7,H0,0 00.24 | K,HPO, 2.8 | Deionized water used in the study was obtained from a Millipore | Milli-Q System. The most recent chemical analysis is in Raw Data Package. | SSppoonnssoorr:Su3dMy CIoDmYpRaInSy04 . 3 000415 EAnvsicroCnmoepnotralioTne/AiSnCtDDiuvliusotnh Ase Say IDA 5010068 Test Setup Ecoxnptorsolusr,e tvwesosetless:t e8x,po1s-uLrebso,roasnidlicfaotuer grleafsesrebnecaekesrusb;sttawnoceintorecautlmuemnts. | f Orderofpreparaion: At 10-minute time intervals: (1) first inoculum choingthrorle,fe(r2e)ntceestsuebxsptoasnucreeecxopnocseunrteractoinocnenrterpalitciaotness,, a(n3)d f(4r)omselcoowndto inoculum control. The final volume in each beaker was 800 mi. Preparation: (1) Added 284 mi of the sludge preparation in each beaker, (2) added 26 mi of synthetic sewage feed to each beaker, and (3) in test and reference exposures, added the required amounts of the test and reference substances, respectively, (4) and diluted each exposure to 800 ml with carbon-treated water. l Determination of Respiration || Incubation and aeration: _19.9 to 20.0C and vigorous aeration. | `Sequence: Same as exposures were prepared. | Determination: At 30-min and 3-h contact times: (1) filled one 300 | ml BOD bottle with the exposure medium and continued to aerate the remaining medium, (2) using a YSI S4A oxygen meter with a selfsmteirdriinugmpervoebrey, 0d.e5temrimniunteedstfhoerDuOp tcoon5c.e5ntmriantuitoensin(thtehefierxstposure determination was made after 0.5 minutes), and (3) the sub-sample bweatswpereonpetrhleysedlisspoofsemde.asuDrOemmeenttesrfcoarliebarcahticoonntcrhoelckosf wtreeraetmmeantd.e ! { Calculation of | Respiration Rate For cach exposure, correlated DO depletion and time interval, and | calculated the slope of the regression line as the respiration rate. | Endpoint Calculation _| ECS0:_Probit Analysis (EPA 1988). | SSppoonnssoorrs St3uMdyCIoDmFpRa1n3y04 000416 . 4 3.0 RESULTS Test Substance Toxicity 30-minute ECs: >1,000 mg/L. (Pertinent data are in Table 1) 3h ECgy >1,000 mg/L. | Reference Substance Toxicity | 30-minute EC50: <3.75 mg/L. | (Pertinent data are in Table 1) 3-h ECs: <3.75 mg/L. EAnSvcilroCnomrepnotraaltiTosni/nASgclDDiviusihon "Ase Sty IDF 5010068 4.0 TEST QA/QC Que Criteria Respiration rates of two inoculum controls must not vary by more than 15%. 3-h ECs, of reference substance must be | between 5-30 mg/L. Test duration must be 3 h. EE Variations after 30-minute and 3-h contact times were less than 3%. 3-h ECs was <3.75 mg/L indicating the | sludge had greater than normal sensitivity. reTshueltl.ack of effect due to R1904 is a valid | `The test duration was 3 h. | 5.0 REFERENCES Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for `Testing of Chemicals. OECD Publication Information Center, Washington, D.C. U.S. Environmental Protection Agency (EPA). 1988. Computer Program Probit. Biological Methods Branch, EMSL, U.S. EPA, Cincinnati, OH 45268. | SSppoonnssoorr: St3uMdy CIoDmApRain5y04 000417 . 5 nECaDACrTIVATEDSLUDGERESPIRATION NHITIONTEST#208 siCsaeoa no CALCULATIONOFRESULTS. 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