Document 91LvbyRQmNqMVVLXZqDD0n7N6
M A S 6 - 0 2 /5
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE________________________________________________
Identity: A mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 502. The test sample is FC206CE. Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. Antifoamer SW S 214 was added at a nominal concentration of 200 mg/L.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD________________________ ________________________________
Method: OECD 209 Test type: Static acute GLP: No Year Com pleted: 1990 A nalytical m onitoring: Dissolved oxygen concentrations, pH and temperature. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro W aste Treatment Plant, St. Paul, MN Test condition:
Dilution w ater: Millipore Milli-QTM water Synthetic sew age: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM
water, then brought to the point of incipient precipitation with 1N H2SO4
and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.2. Test substance was added directly to the test vessels. Antifoamer SW S 214 was added to the test vessles at 200 mg/L from a stock solution prepared by diluting 2000 mg of SW S 214 in 500 mL Milli-QTM water. The solutions appeared miscible in water. Test vessels: Not given. Num ber o f concentrations: three plus 1 reference substance and 2 blank controls Tem perature: 18 - 19 C
001774
Total suspended solids and pH on day o f testing: 7.853 g/L (TSS), initial pH 6.7 and final pH 6.9. Initial TSS in 1000 mg/L concentration was 3.16 g/L, initial pH 6.7, final pH 6.8, and MLSS 3.38. Elem ent Basis: Respiration inhibition as determined by oxygen consumption.
Rem arks: Values corrected to 20 C for calculations.
RESULTS_________________________________________________________
Nom inal concentrations: 2 Blank controls, reference substance, 320, 560
and 1000 mg/L Elem ent values:
30-minute EC50= >1000 mg/L 3-hour EC50= >1000 mg/L
Element values based on nominal concentrations.
Rem arks: There was minimal foaming in the 1000 mg/L exposure concentration when compared to FC-206CE without antifoamer. Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S ___________________________________________________
The FC-206CE 3-hour EC50for activated sludge respiration inhibition was
determined to be >1000 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES__________________________________________________ __
This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number H 1 4 8 0 -4 ,1990.
OTHER______________________ ____________________________________
Last changed: 6/26/00
001775
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
LRJm zULD a te a /^ /q O Analvat ^ ^ -*
Type T eat A rJ W n ri ^ U A d y r^ g & p \.r U c n \^W ibI-fcio^
r^ C ^ ^ Z T fk
Source kU -vtt
}^ V T P k u l } K /tg
D ate O btained
, * ^ / q o ______________ D a te U sed
M ixed L iq u o r S u sp e n d e d S o l i d s (MLSS)
g/L A djustm ent
pH lg .T j-P .H a t loM
Comments
------g/L
T est Substance Sample S am ple pH ^ H t a l
CommentsMLgft dP 1
A n M r a r r v S ' .S U I S ? ! >4
b . f t A d ju s tm e n t___________________________ _
-tosh dn.}
; -ftviaA 'h .t & q ILj
R eference Substance
Sam ple D e s c r i p t i o n ^ S ^ c h l r n f h ^ f t l Vv.ojfVCSt CCMtbBEUO^-tiafcBCl) lOvwjL-
Sa" P l e PM .m tiai h?,P>.) . f i k ^ 1 .Q __________A d ju s tm e n t
Comments
^ M u tt o n , p R *1.2.__________________
Exposure C onditions
T est D uration
___________
T est Tem perature tftv - 1 ^
C
Comment 8_____________________________
001776
Foim ioeao * D PWO
Lab Request No. U K f lQ - L j.
Sample Description
Environm ental Lab -- W ork Sheet
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Page ___ I
of
Date: ^j
Analyst:
5
1
T e s t S o l u t i ons Pr e p a r a tio n
PCZ OE^-LcLSOZj l/vu/gR) .WrlV\^ iriixfot%rifj" S W S A4 LQQni ^ lb )
-tTC-ZOfgC^V.fffcSQZj_\tAri\Vvdu.a.\ V/dr.urtte'_______________________ ___
S W S Z.l*-!-
Z O O vrv^li^ r c e i r .V-o p f t g t 7. r L . i3r. .5>-- rt- S W S . Z . H -
______ ^ q Vu ^ tT v I .
T e s t Cone, mg added m -M tk -----n er 500 ml
c^\r\^r-.h ._______ _______ _
mlMQ w a ter ml s y n . sew age ml m ic r o b ia l n er 500 m l ^ ^ ^ f e e d n e r 500 ml in o c u lim ^ e r
T otal voltine
-3JLQ_______ ILcO__t __ 3LSQ + .5 V __Li/_____ i ____ ZQQ__ _______ E ___S QQ ^
-^LgO ______ .SCS_ __ Z.SCf -h Z S 4- \lp__________L Q Q ______ E ____ ^ 0
. 1 0 0 0 * ______ ^ O Q ... +___2 - 5 A 4- 2 J Z 4-__ LLz_______ t _____Z - O Q -- _____E __ S O
B H C oR im fvS
ur>U V\
| 4 \ 4 f t Q ^ __________________ -
M n v M fU .-fb a m ltiQ .
trW l
r lu p Q c a f M . U ^ r i >uv w t ^ ^ s a r t v ^ - r ^ S
001777
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
RAW DATA
#209
lr JJLiiA SQ zii_______ 2- 'p'5
T est Substance:
ZAbCE
A n-h-fo gyn -p r 5>\aJ S 2 , | 4 l
Source o f A c tiv a te d Sludge Mixed L io u o r : V jfth n n U/ a. s f f .
Sk TRul y^
Date R e c e iv e d :......* / 2 * > / q n ________
Date Used:
___________
A nalyst: ~3^cicheft4>^~^dnlrfp$tA
30 - MINUTE EXPOSURE
le s t substance C oncentration (mg/L) e.5 1 .0
CONTROL I X ? r>
*1,4 l ,5
* . n P ,0
* i Pn 1 or>r>
U, 1.1/9 0 . *
DISSOLVED OXYGEN CONCENTRATIONS mo/L T O T MINUTEs
1.5 2.0 2.5 3.0 3.5 4 .0 4.5 5 .0
,4 ,5 4 , 7 4 ,? . 5 . 1 5 ,1 Z ,`b b i \
1 .4 0 ,5
0 ,5
5 .5 \.k> \ 5
ia Aa Aa
CONTROL I I
> j\
J5u
JL S L .
\R -L _
J
3 - HOUR EXPOSURE le s t Substance
C oncentration (mg/L) CONTROL I
IcO
_ 1000
DISSOLVED OXYGEN CONCENTRATIONS ma/L
tlME-M INUTES
TEI
0 .5 1 .0 1 .5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 .5 .5 C
Iff,5 I f t l
44 4,4
2 ,4 ,Q
1,0 A a
4,5 5 , 4 5 ,, ?,\r> ?,,0 i 4 0,4
ia
,*,4 . 5 , 4 2, ,ft P u li 1. Lo 1,1 0 ,5
19_
->4lo .l ,5 ,1 5 . ^ ,
L 4 ,1 M
5,0 2_i 4 1,4 1,5 0.8 J 3
CONTROL I I
*5 _ ia _
pH o f A c tiv a te d S ludge M ixed L iq u o r : pH o f h ig h e s t c o n c e n tr a tio n t e s t e d : Test Tem perature: IQ -1 9 C
3l
I n i t i a l Ip*! I n i t i a l l$,*1
J J 1 ,1 Z j- U 5 _
Final la ft
F in a l U>B
M
001778
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
T e s t Rnlvstanpp PC ZbdE.
lljfi)Xr^lnrT^r S W S 2 \4
L R J dSC b! Date
Analys' P3
.)
30 - MINUTE EXPOSURE
T est Substance C o n cen tra tio n (mg/L)
L_L
i b iL IOQ.Q
Oxygen Uptake Rate __________R'
JLlJjtL L i k .521
JLQ a *
R C orrected (1) to 20 C
U SO S.
L fts a i.
* In hibition in R espiration R ate^
k
QM QUI
laai
IP e r c e n t D if f e r e n c e i n C o n tr o ls (3 ) EC5 0 (951 C o n fid en ce L im its ) mg/L,
jb L S f .
T est Substance C o n cen tration (mg/L) CONTROL I -O
, in n n
Oxygen Uptake Rate R
1. H i o S 1. I S 4 3 'l. 03*10
l.& S ffl
R Corrected t o 20 C
I.2 O ?)
1.Z 2 \Z *\
\.7T , p n i.umn
%In h ib itio n in f2l R espiration R a te ^
n,s
T P fT 0.
N
CONTROL I I
________10 * 1 l a l __________
P ercent D ifferen ce in C ontrols (3)
K ^Q (95% C O T fidence L im its) mg/L.
TMr nhc (1 ) Temp. C o r r e c t io n ! --
e
(mg 0 2 /L - m in .)
yav^'
(3 ) I D if f e r e n c e -- ----------f x 100 ( RC1 + RC^
f c ; rc2 \
(2) % I n h i b i t i o n ! j A , x 100
K * RCj
The two c o n tr o l r e s p ir a tio n r a te s sh o u ld b e w it h in 15% o f each o th e
Where: R * Oxygen u p ta k e r a t e (mg 0 2/L - m in .) T ,o b s Sample tem p.,*C f t - 1 .0 4 7 , x - T ,o b s - 20*C C Control
v\sb c a lc u la b le .) *M e n . deptebecl -boo ra^dVv^ ^
1=^ -th v t 1 ^ ^ 903/7 7 9
Type T est
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
L iU M m = 5 L D ateato V qO A n a ly s c~~ J ts i
S o u rc e M ^ o ( i j k ^ ^ g A ^ g K f T T n a j ^ . S A T T a u D J M ia------------------- ,-----------
Date O btained
J * > / Z 3 / q o _______________ D a te U sed _____X ) Z & J c \Q _____________
M ixed L iq u o r S u sp e n d e d S o l i d s (MLSS) *"f .R 5s*> g /L A d ju s tm e n t
g /L
Comments
~ ..
T est Substance
Sample D e s c rip tlo n jS W lfi
( M/*1 jftltA ^ .W c O A ts s
Vvi^vceA-c o w ^ b ^ o W -tea^atij lO o o V r u y U 1
*
. A drianV i "
Sample pH J ^ M f c n ,
t a .g ______A d ju s tm e n t______________________________
Comments
. p U U .*1.
_____ _________________
R eference Substance
Sample D e s c r ip tio n . ^
-._____
Wa^sst eeei^eW ow>4 s *i*lJ lOmjU
Sa P l e PH-liAftiai I g . ^ f i n a l 1*.C>_______ A d ju s tm e n t
Comments
'I . I , -------------------------
Exposure C onditions T est D uration f t T e s t T e m p era tu re 1f i - 1 ^ Comments___ ________________
001780
m 1082d -a -O -P W O
impie Description
Environm ental Lab -- W ork S heet
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Lab Request No.U \4 fiQ -c S
P a g e ------L - -- of ..JS . Date: __ Analyst:
___________________________ S to ck and T e s t S o lu t io n s P r e p a r a tio n _____________________________
A irtfcH b am er .^ / l / B b ) S U ic o n g s C s r y r a B c r i ^ A d r i a k t } i ^ . ____
STOCK SOLUTION:____2 0 0 0 ^ / ^ 0 0 ^ 1 >
_______ ________
_______________ - ? n n n w i^ 5 > v f .s x t 4 / ^ Q Q v v i L j
______
------------------------------v e r u m i s c j b \ g ^ ^ .
,a c M .
ItH .
a s t Cone. m l s t o c k s o i n mlMQ w a te r
p er 500 ml
p er 500 ml
ml syn . sewage ml m icrob ial fe ed jjje ^ jiO O n n ^
T o ta l volume
IS O O
I2 J S _____ ____LS9______ ______ L k _______ _____ZOO_________ = /S f l
%Y\rnr& TFVj foy-tfan-A, w'^Vi
U '4Pfl -jvU.
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIO N TEST
RAW DATA
#209
L R jM ffl-5 (Tage. Z. 4 5
T est Substance; 5 W 5 2.14
(_n h Itti^
Source o f A c tiv a te d S ludge M ixed L iq u o r ; KJ f H r \
i f lM g s ('k n p o r a tia M j - A d r i a ^ M j .
} ^ 4 - fT m >0 1^1 n
Date R e c e iv e d ;. ' J z & f a t ) __________
Date Used;
___________
A n a ly s t!
30 - MINUTE EXPOSURE
le s t substance C o n ce n tra tio n (m g/L) 0.5 1 . 0
CONTROL I
1.4
1 2>.0
1 .5
2 .0
2 .5
t o t m N T
3.0 3.5 4.0
lift
.
4,1
H.2,
ft.l
4.5
$.0 .1
5.5 ?
\.l
Ifi
AS.
CONTROL I I
U..5C .& S _ a . a .
\,4
rvQ
Ifi
3 - HOUR EXPOSURE
le s t Substance
tlM E-M INUTESl
TEf
C o n c e n tra tio n (m g/L) 0 .5 1 .0 1 .5 2.0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 1.5.5 C
CONTROL I
^0(1
4 8 4 ,4 ,
ft, 2 L , 4 2 ..0
1 .0 1 8
1 r n n
2L .2 - I.S T 1.
18
--
CONTROL I I
5 .6 4 .8 4 + 3 .
z .n
pH o f A c t iv a te d S ludge M ixed L iq u o r : pH o f h ig h e s t c o n c e n tr a tio n t e s t e d : Test Tem perature: 1 8 -1 ^ ^ ,
I n i t i a l U>n In itia l if / I
. 4 1.1 \ - 2 . a s
F in a l U> 8 F in a l l0 * 8
ja
001782
EWIIWMnWTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
T e s t s.ih < ta n r^ \> J 5 > 2 1 4 A ^ f o a n r V > r ( ^ P i n ) crv\tn
30 - MINUTE EXPOSURE
T est Substance C o n cen tra tio n (me/L)
lo o n
Oxygen Uptake Rate R
l. .
i.n m q
R C onrected^ t o 20 C
1*1 ftO
i.lfW Io
L R _tiJ S aS-
PateJS jIzd S Q .
A nalyst
,
% In h ib ition in rr R e s p i r a t io n R a te *-2
!.>
OW BHPLH__________________
i . ' m i _____________
P e r c e n t D if f e r e n c e i n C o n t r o l s ^ ______ 4 . 8 ^ ) EC5 0 (951 C o n fid e n c e L im its ) m g/L ______________
T est Substance C o n cen tra tio n (mg/L)
CONTROL I in n n
Oxygen Uptake Rate R
i.iq sq l.lS S ta
R C orrected^ t o 20 C
\ . 25&Li
t.Z.101
%In h ib ition in R e s p ir a t io n R a te 1*''
CONTROL I I
\.o *ib n
Percent D ifferen ce in C ontrols ^ E C ^ ^ 9 5 \J Z o n fid en ec e Limmii1ts ) mg/L.
(1 ) Temp. C o r r e c tiinonn *s . b s
(mg 0 2 / L - m i n . )
(2) I I n h i b i t io n r' * / ....... x 100 K * RCj
Where:
RR - Oxygen uptake rate (mg 02/L-min.) T,obs Sample temp,,*C L 6& 1.047, x - T.obs - 20*C
C Control
l .lf lQ f o ____ ;_____
IRC-. * RCj (3 ) I D if f e r e n c e r-~ -- ------- X 100
___________
The two c o n tr o l r e s p ir a tio n r a te s sh o u ld b e w it h in 15% o f ea ch o th e
00178
.Solids [TSHTSSHTDS] 3M Environmental Laboratocy - Raw Data Sheet
filth 0 ax -IS to t-T T , Sec. 5*40 O-
_
Method Reference: Std. Methods(14th ed.)pfr. 80-08, sec. 308 M e tro VJkste,T r e a b r i e v & ^ a v t t
h K, ,Mv\
mg residue x 1000 Calculation: mg/l Residue =
m>***pl9
NU'jCeo UouOV^ (ptotS tK cdo-ufifldr 3 /2 3 /^ o )
q |4 8 0 - AJ & * 3 )5 -0 0 ?
Sample Description
UHial
KeWoU0te , IVl'AlUAn^.'PlAuk
.1
Ot.P&ul j Mrv-
* 2,
Reported Result mg/l
3 w tiS n x T csC cfc
'is s s ra ft:^ 4
5
s a ia K s a ^ v f > jbowJ l. ' I
_____________ a
^ - .p c ro w. jb ^ coo**(c -vat A*<Pvi_____ 10
_____________ l i
g& a"6 nn<A.I3
wfc.aoUdBo*"' _____________l i
- ____________IS
ml Sample
Gross Weight after 1 0 3 *c
Weight (g|
8
s 5 5 .8
.8 .8 8 8
8 8 8
<
.8 .8
i . \ 8M q 1. 1 8 8 8
I.lb 4 .4 1 .1 4 1 8 l.liL fi
1. \ 3 l o S
l.1882. 1 .1 3 3 *4
' 1, t i S i ? \.\3 ? > 4 i . lA *r< ; 1. 1 8 2 2 . 1 .1 .3 *8
|.l? S 0
l.U S Ifl
\.\m
I.1Z.S2,
U Z .08 U 2CR M 2DU
1.11^4l.in s ' 1.1184M ill u rn u ia b 1.1184 1.1184 . I.ltio
Residue Weight (g) 0 .0 8 4 8
n.nxnij
o .0 ^ 8 3 O .n iS S
0 . 01S T
O .O L 8 4
o .o is q
0 . fille d 0 . 0 lta 8 O .O llz>4
D .O l/rtQ n .o n o
Residue mg/l
Comments
T fi4 0
"l&lnO
.8 1 0 0 *3 1 4 -0
3 \ fin 3 \IaO
"N
y
5^ - X - 3 l 4 0 . i 4 0 w^ / l
t.s ? _/ I
S .\4 0 .3 S O O
X = 5 \{p O L > v w c /U
y/
^ /= 3 3 4 0 - 'a 0 w te lL 'O
S ^ 8 ( ~ 3 5 Z 0 J O v ^ U
BEST
; 0 E y a v a i l \B L E
c o n g if-
