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HAZLOTON -mgm-@Av-L,Ec,s,, 3301K SMAN BLVD. P.O. BOX 7545 - MADISON, WISCONSIN 53707 Chemical& BioMedicalSciencesDivision PHONE (608)241-4471 TLX 703966 HAZRAL MDS UD F J,14AL REPORT F Pi-)i;FP PFR@,'T@4!:; rijt4@4FcnTA rIJ@JTtIG TOXTt7Ol-Of7,'@' SFR@)ICES .ST. PAUL., t'lN 5c52Ol MANUFACTURING COMPANY SArlPLE: T-40-16 PI-IRCHASE ORDER NUMBER: TP,77389-410 712 SAMPLE NUMBER: SAMPLE ENTERED: REPORT PPT.NTED: 7010031,@,-7 01/'07,,S- F t,Cl L 0 S F,17): ACLJTE ORAL TO"'-"ICITY STUDY PAIAI DATA APPENDIX IN RATS PLETHOD,, SUMMARY., PATHOLOC@'i' ,/ ........... STEUEN M. GLAZA i STUDY DIRECTOR ACUTE TO'@llI COLOG'T' ......... DATE .......... BY At4D FOR HAI-7LETON LABORATORIES AMFRJl-7A, INC. PAIAIDATA FOP THTC, STUDY gt,lFP117A". l@@C. , MADISON, ARE KEPT O@4 FILE LITSCON--Ilfl. AT HAZ7L-ETOt-A LABORATORTEC HAZLETON LABORArORirzs AftAERicA, iNc 3301 KINSMAN BLVD. - P.O. BOX 7545 * MADISON, WISCONSIN Chemical& B!oMedicaSlcienceDsivision 53707 - PHONE (608) 241-4471 - TLX 703956 HAZRAL MDS UD tALIrIF-.:FP.,: 70101@71;7 t,lP'L F T-4016 pA(-F-, Af7lJTF ORAL Ti-iX I f--T T@f' Oh .1e r7t ) k@p-: Tc- determine the acute oral toxicity produced when a test material is admini--,ter-ed bv the oral route Coavaoe) to rats according to the U.S. Environmental Protection Aqenc,,,'s Guidelines for Testing Pe!--.ticirle-- and Toxic Substances. Test tlaterial: T- .40 1r@, Ph-,,-,isc a I Cies c r ip t ic,n Pi ir i t k, An r, C@t Thick- brown liquid a-,5uries stability determinations. pur, t-- 7:t-,- TF--s t Ari i ma I strain Young adi-ilt male ijer-e procured, and female maintained albino by sex rats of in group the Spraque- cages in tempe ratijre- and I,r) Pijrina Roderit @iumidit@)-controlled 17.hoi.,aind Liater and quarters., held @ or provided continuous an acclimation per-iod at least 7 dak,,s. access of ,L'4r-climated animal@@ 6iF--rrzchosen at random for the study. Test animals i.,ier-Phoused by se-, in groups of fi,,je and identified by animal number and r,;orrespondinq tht,nua@ic,ut the ear taq. stud@,,, Food except and for water were an overnight akdailable period ad iust libitum before test material administration i,jheri forid., but not water, was withheld. F,P-a=,on @or Species c-electicin: T@ie rat is the anirrial classically used due to itc, small sizr--. read@,., a,,)ailabilit,,,,, and large amniint of back-.groijnd data. t rl Fi,,.,e rrialr- ar:d @F-m;:@le rat--. @r,r a sinqle d,-3saqr-- le,.,el n4@ 5.0 +r-- '-c)-7 q Pt--eparation and calculated and administered Admirii--tratiori of Test rlaterial: for each animal based upnn it-. iindiluted b,,, ga-,)ao,e. An individual d05e fasted body welqht The do-le ,,,olume 1_jric,nari awerarie of t@-ie test Liulk matc-r-ial of 1.06 was o /rT, I . rril,'kq of bod@,) weight basp-d fr)r R'oiite of Administration: a @@rioi,inouantitt,) cif tc-7@t substance This and is t[-ie method for @-ias been t@-ie route administering of choice . I- 0'b@* HAZLOTON 3301 KINSMAN --sc@-Es .. BLVD. P.O. BOX 7545 o MADISON, WISCONSIN Chemical & BioMedical Sciences 53707 o PHONE (608) 241-4471 TLX 703956 HAZRAL Division MDS UD -cl;-@rlPLF N!-IrlBFR: 7Fill@03177 pAf;F r1p 1,,17- -7- Af'-,I-ITEOPAT- TC-I'l-lif7TT'i' COt4T TNIJER.) Ob F.e r @,a t i o ri@.: The animals wer-e obser,,,ed for clinical 5ions and mortalit@,l at *11. and 4 hours after test material administratic)ri. The animals lAlf@,rto@bsert,.,ed thereafter for 1.'4days for clinical 5ioris and twice dail@,) for mortalitk,,. All animals i@ip-rci-,lel.cihediust before .Clijr,,,,i,,a,njinmca,ls wer-e weia.@ied again test material adrciinistratior.. at -7 days and at study termination. Patholog@.,: ;-ir,imals At stijdk) termiriatior, sijrvi,.,,inganimals were euthanatized. died durinq the study or were euthanatized recei,.,,eda necropsk.1, examinati.ort and all abnormalities werr-- recorded. H Az LUTON 330K1INSMABNLVD-.P.OB.OX7545MADISON, WISCONSIN 53707 Chemic&aBl!oMediSccailencDeisvision PHONE (608)241-4471 TLX 703956 HAZRAL MDS UD SAt,lP@ F: T - 4,4Ci'Ifg,@ 77 PE ACI l-7E !-IPAL TO@,'lC ITl,' SUMMAPY CL-ITJTI NLIED Test i:-inimal: Albino Rats - I-prao,ue-Dawlek,,lstr-ain Source: Charles Rl,,,,erI--aboratories, Inc., Portaqe MT 1 [late @4riirrialER.ecei,,.)ed: tIF-thorJof 4)dmiriistrat ion: Oral Ga@.,ane [@@-te Test Started: Date Test 1--ompleted: 01 Estimated Oral LDC50: Male - Greater than r-.0 q,,*kq of bodk., weiqht Female - Greater than c7,.O c3/@-,qof body weiqht Dosage Lewe I Male 0 FF-rriaI e 1-@ A@.,f--raqe Bod,,) ltleiq@its(q) Initial Da@,' 7 Terminal 264 2 li9 321; 1,I'@, 3(,l @3 , - 44 Mf:)rtalit,,,j(Number Dead,"Nijm@er Dosed) 01,15 11,15 Hi-)iir s Clinical Sions 7 C@, Da @)s -1 '1, '1 @'laI e- P,r-jpeared Norma 1 4 4 5 F, 17@ 5 71 @7, 5 F 15 H. Fjr-:t 1 j t 0 0 0 ID ID ID 0 0 0 0 0 0 0 0 ;'ed-stained f ;:17p- 1 0 0 0 0 0 0 0 i) 0 0 0 0 0 cl r:e s E-, ,.,e s a i @.,at ion 0 0 1 00 000 0 0 0 0 0 0 0 0 Ferria Ie s i "Z r-, t i t k',, H g in e d a c-.e 44 44 I 1 0 0 0 0 ID 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 F .1a@ I i,,,,ta i,on t a.@:1 a Fr,,,3icat ion a t @-, 0 00 0 0 0 0 0 0 0 0 0 0 0 C, 0 0 0 0 1 0 00 0 0 0 0 0 0 0 0 0 0 iD 0 01 0 0 0 0 0 0 0 0 0 0 0 @i ci 0 00 1 0 0 0 0 ID 0 0 0 0 0 Fi-iiintd@p-ad in Fi.M. HAZLOTON LABCNXVRIES Atl,*RICA. irC Chemical& BioMedicalSciencesDivision 3301KINSMAN BLVD. o P.O.BOX 7545 - MADISON, WISCONSIN 53707 - PHONE (6082)41-4471 TLX 703956HAZRAL MDS UD t4I-1M E?F.F-':7 fi1ID1-7,F T - .-r,i r, F i@:F, r-- A'-1-ITE OF-'tL@L-Tl-l'*I-C' IT'i' Dc,sacp Le@,r--I An irriaI r4ijri)bre S F-. ri c 3 el0 Cr. 43 c F, F C@ 5 2 F F -7 1; F I-r@@ F, 'I C.7 F COT-AT I NIJEE! P A T H IDL r@7 ci,k,r3 o f b o d Te@zt Da,,) Died Sacrificed we Iri@i Necrops,-j Ccimment@@ C @ - - - - @- c c - t-a i ri@i d firm, rubber@,1,, porous r materia Stomach @ i r m., - corit a i rts derk,-brr,)i@in r u b b r= r,.,,), p ri r o u s rria t P- r a a 14 Stomach - contains dar-k,-Lirot@ir@ firm.. rubbery, pn-rr7sus material. Stomach - contains dark, fir-m., rubber-,,,),p,orous materiF-1. Stomar-,@-i- cor-itains dar@-.-brrji,ir. @ir-m, rubber,,,,, por-nus material. Stomach - contains dark- -b r-oi@it-i @irrri,,rubbery. pornus materia ulcerated arr--as on nono,lanrJ;Alar- D n , t , - ;. t riMe3 C:(Dr-it a ,n -@ d a r @jr r-iL@ir-) rubber@,,,, pc,rou-- meteriai. t (Dma r.@-i - c 1,r3-ti a in@@, I r-m , r iib b r-r-,,..@. cic,roij@E, rnigteria 'i Storriar-@-i- r-(7jr)tien,-. i r rr,i r ;jb b e.r,",, r)o r ciii n-iat e r )a ulcerated areas or-,riorir3latidiilar p c,r t i r3ri. Strjrnar.F,- cf-intairis dar-k-br-rii,in f i rrri,,rubbery, por-cj:js rnater ia a t-di F-x- h ib ite d d I ff 11.e5@I r e id a r-e a s ciri q Ia n d i-i',a r p c,r t i rDri. F,n.r@, iiI r-e-,r a t F-ri ar-L-aE-, or, o laridu Iar and n.--IngIaridii lar .or,r-,ti i o ris . @@.-'HAZLGICCLANBOPATORIES AftAERICA.INC Chemical& BioMedicalSciencesDivision 3301KINSMAN BLVD. 0 P.O.BOX 7545 - MADISON, WISCONSIN 53707 - PHONE J608)241-4471- TLX 703956HAZRAL MDS UD pA -F t4l-lrlRFP: l@I n 1--,.c7'@ - : T -.4 f@,l@ Ai-IJT=I- t)P'H'I- Tf7l,,'Tf-TT@' (COt4T I TAUEE, Rp@erenc:es: i H,@ t P. 11". Ar--itir--Ora I To x ic i ijdkl,"t,.Re.-,t i r-i-de Asse5.smen t ,,Iji de I inF--s @-;u@jriJ-,j)s ion F Haz a rd E,,)aIua t ion Human and Dorpes t i c (-@n Is , IJ.1-n. Enk,,i rcinrrir-t-an I Rrn t er-t- i-nn AqF.--nc-,@Of f i ce o f Pes t Ic ide and Toxic. C-,ubstarice Series 8'1-1, pp. 34-39, No-,.)ember 190-"@'. e-)1 rC7 p r-C@ Laborator@,, r 1 1,, 4-- -j -@@- -tI - - D-lic Adr,-;-,:@=,@-@l;---.@-,'::, Pract ices Peo,ulatiori--@for tionclinical Laborator@j Studies'). 7. DHHC- Pijblicatinr, Nri. (TIIH eF,-23 19P,15) Gijide for thp Carp- and U--,p ci@ Laborator-,) Animals. old Lo -i E a 00 Lt 10 ......... .it rc a 30. cc -So, @Lc C6 a. a is 0 cri to 41 H t- -0 CO z filll@I lI 0 C4 ri 0.0 Ira. Irv. Aj CL t)O CL IU ul I I ltV IW4 en -71 1 11 cc>;1' 114H 11 1 L r*- I IIL cn t I I 111 V Nil I I r@> 11 I Nil1 -1N 11 11 NI ]Ill]]III IN 0 0 -1 0 fl '00