Document 8VM7abRwbExE4Q0JK0MBg05m5

ACUTE TOXICITY TO FISH (FATHEAD MINNOM TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatew,hich may also be referredto as PFOS, FC-95, or as a component of FC-206C and 81-2/8. (1-Octanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecaflpuootraos-s,ium salt,CAS # 2795-39-3) Remarks: The 3M producton lotnumber was not noted. The test sample isFC-206C and isreferredto by the tesbng laboratoryas "81-2/8". Current informationindicatesitisa mixtureof 0.73% PFOS, 76.09% water, 15% diethyleneglycolbutylether,4% urea,1.55% sodium octyl sulfate,1.47% hydroxy foamer, 1.05% polyoxyethylenemonooctylphenyl ether,0.06% sodium laurylsulfate,and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characteilzed concentration of impudties. Data may not accurately mtlect the toxicityof the tluorochemical compnent of the test sample.. METHOD: Method: EG&G, Bionomics protocolenfited"Methods forconducting flow-throughtoxicittyestswith freshwaterflsh". Test type: Flow-throughacute GLP: No Year Completed: 1981 Species: Pimephales promelas Supplier:Commercial supplierinNebraska Analyticalmonitoring:Temperature,pH, DO, and conductivity Exposure period:96-hours Statisticamlethods: LC5ovaluescalculatedusinga computer program (createdby Stephan, 1978) based on the moving average angle analysis, probitanalysis,or binomialprobabilitays appropriate. Test fishage: Not noted. Length and weight: Length = 33 - 54 mm witha mean of 41 mm Loading: 0.38 g/L Weight = 0.25 - 1.53 g with a mean of 0.57 g Pretreatment: Not noted. Test conditions: Dilutionwater: Well water supplemented with Town of Wareham untreatedand unchlorinatedwellwater. Dilutionwater chemistry: Hardness: 22 -24 mg/L(asCaC03) Alkalinity1:8- 24 mg/L(asCaC03) Conductivity: 90 pmhos/cm pH: 6.7-6.8 LightingC:oolWhite@ fluorescelnitghtpsrovidead dailpyhotoperioodf 16 hours lightand 8 hours darkwithan intensitoyf30 - 70 footcandles. Stock and testsolutionpreparation:A primarystocksolutonof681 mg/mL was prepared by adding 2724 grams oftestsample todistilled water and dilutintgo4000 mL. Alltestsolutionswere prepared by a dilutedreliverinfgivenominal concentrationsofthetestsample and controlwater. Flow-through rate: 5.9 aquarium volume replacements per 24 hour period. Stabilityof the testchemical solution:Not noted. Exposure vessels: 39 x 20 x 25 cm glasstanks which maintainedtest solutionvolume of 15 liters. Number of replicates:two Number of fishper replicate:10 Number of concentrations:5 plusa blankcontrol Water chemistry during the study: pH range (0-96hours): 6.8 - 7.1(controelxposure) 6.7- 7.3(2000 mg/L exposure) Temperature range (0-96hours): 21 - 220C (forallaquaria) Dissolved oxygen range (0-96hours): 7.7- 9.0nig/L(controelxposure) 7.1- 8.7mg/L (2000 mg/L exposure) RESULTS Nominal concentrations: Bk control,125, 250, 500, 1000, and 2000 mg/L Element value: 96-hour LC50 = >2,000 mg/L Element valuebased on nominal concentrations. Remarks: Tesfingwas conducted on the mixtureas describedinthetest substance remarks field.The valuereportedappliestothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206C96-houLrC5oforPimephalepsromelaswas determinetdo be >2,000 mg/L,the highestconcentratiotnested.No mortalitwyas observed atthisconcentration. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudy meets allthe criterifaorquality testing.However, thereisno informatiotnoconfirmthe dilutewras workingpropedy. Sample puft was notpropedy characterizeadnd the studylacksanalyticaclonfirmationofthe amount offluorochemical proportioninsolution. REFERENCES This studywas conducted by EG&G, Bionomics ofWareham, Massachusetts atthe requestofthe 3M Company, 3M Lab Request number 7038, 1981. OTHER Last changed: 6/26/00 ACUTE TOXICITY OF 81-2/8 TO 703 FATHTAD MINNOW (Pimephales promelas). FC- 0 6 c- TOXICITY TEST REPORT SUBMITTED TO 3M ST. PAUL, MINNESOTA REPORT-.#BW-81-10-1029 EG&G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham, Massachusetts October, 1981 INTRODUCTION The purpose of this study was 'to estimate. the acute toxicity of 81-2/8 to fathead minnow (Pimephales promelas) under flow-through conditions. Preliminary- range-f inding static tests and a subsequent 96-hour flow-through test were conducted at the Aquatic Toxicology Laboratory of EG&G, Bionomics, Wareham, Massachusetts. The acute toxicity of 81-2/8 was measured by estimating the LC50 value for the comp ound at each 24 hour interval of the test. The LC50 is defined as the concentration of the test compound in dilution water which caused mortality of 50% of the test -animal population at the stated exposure interval. The flow-through test was conducted from 24-28 September 1981 with a two day pre-exposure period. All raw data generated are stored at-the-above location. MATERIALS AND METHODS Procedures used in this acute toxicity test closely followed those described in the EG&G, Bionomics protocol entitled "Methods for conducting flow-through toxicity tests with freshwater fish." Values are reported to different-levels of significance depending on the, accuracy of the Tneasuring devices involved in any one process. The 81-2/8, an amber colored liquid, was received from 3M, St. Paul, Minnesota on 12 August 1981. The test material was stored in the dark at ambient temperature (210 C). The material was tested as 100% 2 active ingredient. Nominal test concentrations are reported as milligrams of 81-2/8 per liter of test solution (mg/k). The fathead minnow (Bionomics lot #8lAl7) were obtained from a commercial fish supplier in Nebraska and held in a 500-t fiberglass tank under a photoperiod of 16 hours light and 8 hours darkness. All fish were fed a dry, pelleted food, ad libitum, daily except during the 48 hours prior to testing. There was 1.0% mortality in the test fish population during this 2 day period (Daily Record of Fish Holding Conditions). The well water which flowed into this tank was characterized as having total hardness and alkalinity ranges as calcium carbonate (CaC03) of 22-24 mg/i and 18-21 mg/t, respectively and a specific conductance-range of 80-90 micromhos per centimeter (pmhos/cm) (Weekly Gravity Feed Tank Water Quality Analysis togbbok). Other parameters monitored in the holding tank were a pH range of 6.5-6.7, a dissolved oxygen .(DO) range of 94-99% of saturation and a flow rate of 18 tank volume replacements/day (Weekly Record of Fish Holding Water Characteristics). Test fish were maintained under these conditions for a minimum of 14 days. The temperature in the holding tank was 210C during this 14 day period,(Daily Record of'Fish Holding Conditions). The specific conductance was measured with a YSI Model #33 salinity-conductivitytemperature meter and probe, the pH was measured with an Instrumentation Laboratory Model #175 pH meter and combination ,electrode, the DO was measured with a YSI Model #57 dissolved 3 oxygen meter and probe and the temperature was measured with a Brooklyn alcohol thermometer.. Total hardness and alkalinity were measured according to APRA et al. (1975). The exposure system used in this study was a modified, proportional diluter similar to that described by Mount and Brungs (1967) with a 0.50 dilution factor. The dilution water was well water which was pump ed to an epoxy coated concrete reservoir where it was supplemented with Town of Wareham untreated and unchlorinated well water and aerated. This water was characterized as having total hardness and alkalinity ranges as CaC03 of 22-24 mg/t and 18-24 mg/t, respectively, a pH range of 6.7-6.8 and a specific conductance of 90 pmhos/cm (Weekly Gravity Feed-Tank Water Quality Analysis Logbook). The diluter delivered five nominal concentrations of 81-2/8, 2000, 1000, 500, 250, 125 mg/L and control water containing no 81-2/8 to duplicate test aquaria. Each glass test aquarium measured 39.x 20 x 25 centimeters (cm) with 19.5 cm high drains which maintained water volumes of 15 k. The diluter delivered 0.5 t of test water to each aquarium at an average rate of 178 cycles per day. This is equivalent to 5.9 aquarium volume replacements per 24 hour period. Illumination was provided by Cool WhiteR fluorescent lights centrally located above the test aquaria. Sixteen hours of light at 30-70 footcandles at the water surface were 4 provided each day. Test temperatures were controlled by immersion coil heaters in a water bath where the test aquaria were located. A 4-L glass Mariotte bottle in conjunction with a "dipping bird" delivery system (Lemke, et al. 1978')was utilized to deliver a stock so1 ution to the mixing chamber of the diluter with every cycle. The stock solution of 681 mg/mi was prepared by adding 2724 grams of 81-2/8 to distilled water and diluting to 4000 mk. The 81-2/8 stock solution was introduced into the diluter and cycling began on 22 September 1981. The test was initiated two days later when ten fathead minn6w @iith"a mean (range, N=30) wet weight and total length of 0.57(0.25-1.53) gram and 41(33-54) millimeters (Fish Weights and Lengths Log) were randomly distributed to each aquarium. Biological observations of the fish and observations of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pH, temperature and dissolved oxygen (DO) concentrations were measured in both replicates of the control, high, middle and low concentrations at 24, 48, 72 and 96 hours and in both replicates of all concentrations at 0 hour of exposure. The concentrations tested and the corresponding mortality data derived from the toxicity test@were used to estimate 24-, 48-, 72- and 96-hour median lethal concentrations (LC50) and 95% confidence intervals. The computer program utilized (Stephan, 1978, personal communication) estimated LC50 values using one of three statistical methods in the following order of preference: moving average angle analysis, pr obit analysis, binomial probability. The method selected was determined by the characteristics of the data base (i.e. presence or absence of test concentrations causing mortality of 100% of the test animal population, test concentrations causing mortality of a partial number of animals in the population, etc.). The computer program scanned the data base, identified the most preferred stdtidtical method and performed the analysis. RESULTS The test material 81-2/8, was not acutely toxic to fathead minnows at nominal concentrations as high as 2000 mg/L (Table 1). Al.though sporadic mortality and lethargy of the fish were observed, there were-...-d.onsoe-response trends suggestive of 81-2/8 related effects. In addition, the control mortality incurred suggest that the mortality and lethargy observed were due to extraneous factors and not 81-2/8. The estimated LC50 values after 24, 48, 72 and.96 hours of exposure were >2000 mg/t,'the highest concentration tested. 6 The temperature in various aquaria ranged from 21-22 0 C during exposure. The pH and dissolved oxygen concentrations measured during the toxicity test are presented in Table 2. LITERATURE CITED APRA, AWWA, WPCF. 1975. Standard methods for the examination of water and wastewater. 14th Edition, Washington, D.C. 11-93 pp. Lemke, A.E., W.A. Brungs and B.J. Halligan. 1978. Manual for constr uction and operation of toxicity-testihg proportional diluters. EPA-600/3-78-072. Mount, D.I. and W.A. Brungs. 1967. A simplified dosing apparatus for fish toxicology studies. Water Res. 1: 20-29. Stephan, Charles. 1978. U.S. EPA, Envirormental Research Laboratory, Duluth, Minnesota. Personal communication. Table l.' Concentrations tested, corresponding percentage mortalities and behavioral observations of fathead minnow (Pimephales promelas) exposed to 81-2/8 under flow-through conditions. Twenty fish were exposed to each concentration. Nominal concentration (mg/y.) 24 hour Percentage mortality 48 hour 72 h ur ..... .... .... 2000 1000 500 250 125 0a 0a 0 0a 0 0 Cl. 0 0a 0 0a control 0 0 .. ................. .... ... . .. a,b 0 0a a 0 15 a,b 5 a,c 15 a Suds formed on the surface of the test solution. b Some fish were lethargic. c One fishwas lethargic. 96 hour a,b 0 5a 0 is 5 15 Table 2. The pH and DO concentrations measured during the 96-hour-flow-throug test with.fathead minnow (Pimephales promelas.) and 81-2/8. The rang ments made from replicate aquaria are reported. Nominal concentration (mg/k) pH 2000 1000 500 250 125 control Do (mg/.t) 2000 1000 500 250 125 control a No:measurement made. 0 hour 7.3 7.2 7.1-7.2 7.1 ..7.1 7.1 8.6-8.7 8.6 8.9 8.9-9.0 8.9 9.0 24 hour 7.1-7.2 -a 7.0-7.1 -a 7.0 7.0 7.6-8.0 -a 7.5-7.7 a 7.8-8.0 7.7-8.0 48 hour 6.8 -a 6.7-6.8 -a 6.6-6.7 6.8-6.9 7.6-8.0 -a 7.8 a 7.7-8.0 8.5 72 hour 6.7-6.8 -a 6.7 -a 6.8-6.9 6.9-7.0 7.1-7.6 -a @:.7.6-7.7 a 7.6-7.9 8.3-8.4 SUBMITTED BY: EG&G, Bionomics Aquatic Toxicology Laboratory 790 Main Street Wareham,'Massachusetts September, 1981 PRINCIPAL INVESTIGATOR: Brenda F. Wilson Aquatic Biologist STUDY DIRECTOR: Gerald' .'LeBlan /uatic Tox@cologist DATA AUDITED BY: Robert E. Bentley Director, Quali@ttyAy/uA urance Unit