Document 8V1z3jvYjxLRZq7dejyLYL3ey
Ad a.a.6 - 0768
BIODEGRADATION (BOD/COD)
TEST SUBSTANCE_______________________________________________
Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-203FC, identified by the laboratory as R1335. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect degradation of the fluorochemical component of the test sample.
METHQPj____________________________________________
Method: OECD Guideline 301D Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days Inoculum: Polyseed NX, Lot D257, Polybac Corporation, Bethelem, PA.
RESULTS_______ !______________________________________________
Chemical Oxygen Demand (COD): Biochemical Oxygen Demand (CBOD):
5-Day: 10-Day: 20-Day: 28-Day:
447,000 mg/kg
Replicate 1 <95,000 mg/kg 199,500 mg/kg 294,900 mg/kg 301,500 mg/kg
Replicate 2 <95,000 mg/kg 224.800 mg/kg 274.800 mg/kg 290,000 mg/kg
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CO NC LUSIONS
No conclusions given.
001333
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Kllmisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution.
REFERENCES_____________________________________________
This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1335, 1997. O T H E R __________________________________________________________ Last changed: 6/26/00
0G1334
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AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Company U 9/97 AScl-DETD Study ID #5010-068
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R 1 3 3 5 BOD REPORT FOR 3M COMPANY
Submitted to 3M Environmental Technology and Safety Services
9 3 5 Bush Avenue Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331
Prepared by AScI Corporation/AScI-Duluth Environmental Testing Division
4 4 4 4 Airpark Boulevard Duluth, Minnesota 55811
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Sponsor: 3M Company Sponsor Study ID# R1335
SEPTEMBER 1997
0<K1335
AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Company - 9/97 AScI-DETD Study ID #5010-068
COD = 447,000 mg 0 2 / kg chemical.
Dates o f BOD test: 8/22/97 - 9/19/97.
Water bath temperature range during the test: 19.6 - 2 2 .0 C (controller shorted out on 9/7/97 overnight, temperature out o f range for about 16 hours.)
Lot number o f Polyseed N X : D 257.
TABLE 1. BOD Dilution Information for R 1335
1 Sample ] ID (mg/L)
13 17 21
Stock Solution Concentration (m g/L)
984 984 984 984
Sample Volume (ml)
2.7 4.0 5.2 6.4
: Ui Sample Concentration
(m g/L)
8.9
13.1
17.1
21.0
TABLE 2. BOD Blank Calculations for R 1335
1 Sample ID
1 Blank 1 Blank H Blank 1 Blank
FIRST REPLICATE
Day \ Initial D.O.
Final D.O.
D.O. Drop
5 8.94 8.79 0.15 10 8.94 8.60 0.34 20 8.94 8.61 0.33 28 8.94 8.64 0.30
SECOND REPLICATE
V1*
Initial D.O.
Final D.O.
D.O. Drop
8.96 8.78 0.18 8.96 8.42 0.54 8.96 8.54 0.42
8.96 8.18 0.78--
Sponsor: 3M Company Sponsor Study ID# R1335
- 1-
001336
AScI Corporation/AScl-Duluth Environmental Testing Division
Submitted to 3M Company - 9197 AScl-DETD Study ID #5010-068
TABLE 3 . BOD Seed Correction Factor Calculations for R 1335
Seed Cone. Day ml/Bottlc
10 5 10 10 10 20 10 28
Initial D.O.
8.92
8.92 8.92 8.92
TM " " I! FIRST REPLICATE
SECOND REPLICATE
Final D.O.
D.O. Drop
SCF* I Initial D.O.
Final D.O.
D.O. Drop
7.10 1.82 0.36 1 8.95 6.81 2.14
6.20 2.72 0.54 H 8.95 5.64 3.31
4.42 4.50 0.90 8.95 4.64 4.31
086 I4.62 4.30
8.95 3.83 5.12
SCF
0.43 0.66 0.86 1.02
a SC F = (D.O. drop * ml seed in test) / ml seed in seed blank.
L Average
SCF
0.40 0.60 0.88 0.94
: O'
Ir 0
TABLE 4 . BOD Reference8 and Tox Blanks Calculations for R 1335
y Cone. R mg/L
Day SCF
Initial
| D.O.
FIRST REPLICATE
Final D.O.
D.O. Corrected
Drop
Drop
BOD mg/Lb
Initial D.O.
SECOND REPLICATE
SC.
Final D.O.
D.O. Corrected
Drop
Drop
I0
5 0.40 9.00 5.00 4.00
3.60
180 | 9.00 4.80 4.20 3.80 9 0
1 210
5
* Reference = dilution.
0.40 9.05 4.24 4.81
4.41
221 1 9.00 3.62 5.38
4.98
ISO mg glucose and ISO mg glutamic acid in 1 liter BOD dilution water, tested at 2%
49
--
.7
.
. :
b BOD mg/L = (corrected D.O. drop) * 300 ml BOD volume / 6 ml reference.
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001337
ASc! Coiporation/AScI-Duluth Environmental Testing Division Submitted to 3M Company - 9/97 AScI-DETD Study ID #5010-068
TABLE 5. BOD Calculations for R1335
H mg/L Day SCF Initial
D.O.
8.9 5 0.40 9.00 13.1 5 0.40 9.01 17.1 5 0.40 8.97 21.0 5 0.40 9.00
FIRST REPLICATE
Final D.O.
D.O. Corrected
Drop
Drop
BOD mg/kg*
8.11 0.89
0.49
--
8.04 0.97
0.57
*
7.30 1.67
1.27
--
7.42 1.58 1.18 <95,000
Mean <95,000
Initial D.O.
9.03 9.03 8.99 8.97
SECOND REPLICATE
f
Final D.O.
D.O. Corrected
Drop
Drop
BOD mg/kg
7.84 1.19 0.79 - J.
7.92 1.11 0.71 .' t- --
7.61 1.38
0.98 ' v --
7.30 1.67 1.27 <95,000
Mean -<95,000
8.9 13.1 1 17.1 I 21.0 1
10 0.60 9.00 10 0.60 9.01 10 0.60 11 8.97 10 0.60 1[ 9.00
1
7.34 7.16 6.61 4.21
1.66 1.85 2.36 4.79
I 8.9
1 131
K 17.1
1 21.0
20 0.88 11 9.00 5.30 3.70
20 0.88 11 9.01 4.62 4.39
20 0.88 8.97 2.96 6.01
20 0.88 9.00 <0.50
-
1.06 -- 9.03 7.28 1.75 1.25 - 9.03 7.04 1.99 1.76 -- 8.99 6.94 2.05 4.19 199,500 8.97 3.65 5.32
Mean 199,500
2.82 316,900 9.03 5.51 3.52
3.51 267,900 9.03 4.31 4.72
5.13 300,000 8.99 4.14 4.85
-
-
8.97 <0.50
-
Mean 294,900
1.15 . -- 1.39 1.45 / 4.72 224,800
Mean 224,800
i*
2.64 ?56,600 3.84 293,100 3.97 .232,200
- 2-Mean 274,000 |
I 89
28 0.94 1 9.00 5.18
3.82
13.1 28 0.94 9.01 4.34 4.67
17.1 28 0.94 8.97 2.66 6.31
U 21.0 28 0.94 1 9.00 2.10 6.90
11
2.88 232,600 9.03 5.80 3.23 3.73 284,700 9.03 4.41 4.62 5.37 314,000 8.99 2.80 6.19 5.96 283,800 8.97 1.42 7.55
Mean 301,500
2.29 .257,300 3.68 280,900 5.25 307.000 6.61 B 4,800
Mea 290,000
* BOD = corrected drop x 1,000,000 / test concentration.
Omitted data points; D.O. drop corrected for SCF too low (< 2 .0 ). Final D.O. < 0 .5 0 mg/L.
Sponsor. 3M Company Sponsor Study ID# R1335
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