Document 8Rqy7zNxnKnOKdxKejk1Q05KK
TRADE SECRET
AR226-3175
DuPont-6010
Study Title H-24516: Static, Acute, 48-Hour Screening Test to
Daphnia magna
Laboratory Project ID: DuPont-6010
AUTHOR: Robert A. Hoke.Ph.D.
STUDY COMPLETED ON: March 12,2001
PERFORMING LABORATORY;
E.I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine 1090 Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
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Company Sanitized. Does not contain TSCA CBl
H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna
Substance Tested: Synonyms/Codes:
H-24516 Haskell Number 24516
Composition:
DuPont-6010
Known Impurities:
Physical Charactarisdcs: Pale to medium tan waxy solid
Stability:
The test substance appeared to be stable under the
conditions of the study, no evidence of instability was
observed.
Solubility: Negligible in water
H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna
Sponsor:
E. I. du Font de Nemours and Company Wihnmgton, Delaware 19898
U.S.A.
Stndv Initiated/Completed: February 20,2001/March 12,2001
m-Life Initiated/Completed: February 20,2001/February 22,2001
DuPont-6010
-3Ceropany Sanitized. Does not contain TSCA i
H-24516: Static, Acute, 48-Hour Scresniag Test to Daphnia magna
DuPont-6010
SUMMARY
The acute toxidty ofH-24516 to the water flea, Daphnia magna (less than 24 hours old), was determined in an unaerated, 48-hour, static test
The study was conducted with 4 concentrations ofH-24516 and a dilution water control at a mean temperature of20.0C (range of 19.9-20.1C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and all test concentrations were clear and colorless with undissolved test material visible in all test chambers except me water control at test start. All water quality parameters were within acceptable limits during the exposure.
Exposure ofdaphnids to me water control and nominal H-24516 concentrations of 1,10,100, and 1000 mg/L resulted in 10,10,0,30, and 50% immobility, respectively, at the end of 48 hours. Nominal H-24516 concentrations were used for calculation ofECso values.
The results are summarized as follows:
Nominal concentrations of H-24516, mg/L 48-hour ECso (95% confidence interval)
for H-24516, based on nominal
concentrations, mg/lf
dilution water control, 1,10,100, and 1000 965 (576 to 3139)
H-24516 exhibited low concern for aquatic hazard1'in an unaerated, 48-hour, static acute test using me water flea, Daphnia magna (less than 24 hours old).
a SAS User's Guide, The PROSIT Procedure, SAS Institute Inc., Caiy. North Carolina, pp. 639 - 646. b Smichek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA:
methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTMSTP 1179. (W.G. Landis, J.S. Hnghes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials,
Philadelphia.
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.Company Sanitized. Does not contain TSCA CBI
H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna
DuPont-6010
CERTIFICATION
We, tile undersigned, declare that this report provides an accurate evaluation of data obtained
fiom this study.
Work Completed by;
B.S.Date '^^w^O^ ^S^S^f^_________ Daniel J. Peteison, Associate Scientist
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Issued by Study Director:
^WU/- /( - ^Ct-
RobertA.HokB.PhJ). Senior Research Scientist
/(3 ^fOM^, o^6/
Date
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Company SanjtEzed. Does nof eonfain TQ^A r-eff
H-24516: Static, Acute, 48-Hour Screening Test to Daphnia magna
DuPont-6010
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-24516
Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
PH
Test Completion (48 hours)
Dissolved
Oxygen
(mg/L)
pH
Water Control
8.8
7.4
8.7
7.5
1
8.8
7.4
8.8
7.5
10
8.8
7.5
8.8
7.6
100
8.7
7.4
8.8
7.6
1000
8.7
7.3
8.8
7.5
TABLE 2
IMMOBILITY OF Daphnia magna, IN AN UNAERATED, STATIC, ACUTE, 48-HOUR TEST WITH H-24516
Nominal H-24516 Concentration (mg/L)
Water Control
1 10 100 1000
Number Immobile/
Number at Study Start
24 Hours
48 Hours
0/10 0/10 0/10 0/10 1/10
1/10 1/10 0/10 3/10 5/10
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Does not contain TSr/ ^0(