Document 8RpKmoNMmgakR6Q4Qpv2EYvDy

AR x a 6 - O g J-6 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) JE ST.SUBSTANCE_________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600. Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: Not noted. Test type: Acute static GLP: No Year Completed: 1975 Species: Pimephales promelas Supplier: Not noted Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: LC50 values graphed. 95% confidence intervals calculated by probit analysis. Test fish age: Not noted Average Length and weight: Length = 2.0 inches Weight = 1.5 grams Loading: Not noted Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered water, City of St. Paul, MN. Dilution water chemistry: Not noted Lighting: Not noted Stock and test solution preparation: Test solutions were created by direct weights additions. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted Exposure vessels: Not noted. Number of replicates: One 001992 Number of fish per replicate: 10 Number of concentrations: 5 plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.0 - 7.2 (control exposure) 7.3 - 7.4 (2,000 mg/L exposure)* Temperature range (0-96 hours): 72F Dissolved oxygen range (0-96 hours): 5.7 - 7.0 mg/L (control exposure) 4.0 - 5.7 mg/L (2,000 mg/L exposure)* * Values for the 2,000 mg/L exposure (third highest concentration) were used because total mortality occurred in the highest concentrations tested. RESULTS___________________________________________________ Nominal concentrations: Bk control, 1,000,1,500, 2,000, 3,000 and 4,000 mg/L Element value: 96-hour LC50 =1,700 (1,465 - 1,972) mg/L Element value based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS ,__________________________________________ The FC-600 96-hour LC5ofor Pimephales promelas was determined to be 1,700 mg/L with a 95% Confidence Interval of 1,465 to 1,972 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DAIA-QUALLDi________________________________________ Reliability: Klimisch ranking 3. The study lacks proper documentation of the method followed. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions. REFERENCES_____________________________________________ This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1975. .OTHER____________ Last changed: 6/28/00 001994 740101 ENVIROIMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET t * TM t--- %-HouP Stiri? T.t o r w i u Tatlisnni Minna,). Date Started ft- S -1 5 _______ Mt.rii Tm ft-Ang _______ * t. .ight /.sq st Zlhek ^Date Completed 3 -3 *1 -7J" environmental engineering laboratory AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET Date Started Time Started: g- as -nr DS 0 -4AM) (PM) Type Test : (Static) 96Exposure Period:_________ -Hour Material Tested: TG--OO Test Organism (Fathead minnow) \JoAvg. Wt. (gram) Avg. Size Date Completed: (inch) -1,5' Diluent :carb o n -filt.-St.P . City Wtr.) Analysis by: (1) K No. of Doses Plotted 1 2 3 4 ! Observed % Cone. 1 Mortality 0.dDeoMll______ _____ VoM tlOOD 40 S H ood loo (2) Expected % Mortality n 4o Bo 3 (3) Corrected Values 5 .3 . cm > 0 -E ObservedExpected ' ' lt.S > Contribution to chi (from Nomograph *11 ff n.n - -< -- ^* iso vW;:.; ' 5 6 7 K Animals/Dose * - JtSL Total contribution to chi gfitf, Total Animals __ 40 _(chi2) contrib. to chi x Tot. Animals chi2 (P-.05) for (K-2) Q_______ degree of freedom (from^Table 2) sai ____ ___________ is less than JSL therefore, the data are not significantly heterogenous. LCg4 3 ___ Slope function (S) n n95% Confidence Limits for LCc* LC50 V l - P o W t 1 lc16 - _____ U 84/LC50 * ^ S O L^Cllfi \iooAafu * 5 o ^ a |li ^ Total number of animals used between 16% and 84% expected effects (N') .2 .7 7 /ifi' fLC50 (S) Ziu^vA-A iiM . (from Nomograph #2) \,I k ^ s o ' ^ s o m lower liB it * & .{& y .U h . \A L5 j m i ^ 5 0 X ^LC50 " uPPer lim it * - . U l Z iEftE LC50 (95% confidence limits 5 to 40 .(1) Do not list more than two consecutive 0% effects or more than two consecutive 100% effects. -~'- (2) Expected value for any dose should be greater than 0.01% or less than 99.99%. (3) Corrected value for each 0% or 100% effect (from Table 1). . * There is a 95% chance that the true value of L C ^ lies within these limits. 0 0 ii 9 99.