Document 8RQOp53EJk2kqGE2xmNq3pNgo

\ \ INFORMATION FOR DR. KEEOE ON THE STABILITY AND TOXICITY,ON BAL-INTRAV. 1 Sta b ility BAL-in t r a v ( BAL-g lu co s id e ) Is a compound o f somewhat' in d e fin it e co m p o sitio n n o r m a lly Is o la t e d , as a bar iu m s a l t . I t has been the p r a ct ice In p h a r m a cologica l s tu d ie s to co n v e r t t h is .b a r iu m s a l t t o t h e fr e e g lu c o s id e b y th e a d d it io n of d ilu t e s u lp h u r ic a cid u n t il bromothymol b lu e , used as an e xt e r n a l in d ic a t o r , give s a ye llo w co lo u r . A ft e r r e m ova l o f b a r iu m s u lp h a t e , fr e e BAL-glu co s id e may be i s o l a t e d fr o m t h e f i l t r a t e b y fr e e z e d r y in g . The s o l i d BAL-g lu c o s id e I s a b r o w n is h h y g r o s c o p ic p ow d e r . The fr e e SAL-glu co sid e , in s o lu t io n and a ls o as the fr e e ze d r ie d s o lid , h a s been fou n d In our la b o r a t o r ie s to be s t a b le , a t room tem p er atu r e fo r a t le a s t s e v e r a l m on ths, a ft e r w hich tim e th e t o x ic it y and a n t i- a r s e n ic a l a c t i v i t y have n o t been s ig n ific a n t ly a lt e r e d . T o x ic 1t y and Ac t l v l t y The LDi-q fo r i n t r a p e r i t o n e a l i n je c t i o n In m ice v a r ie s r a t h e r w id e ly , b u t r e c e n t fig u r e s fr om our la b o r a t o r ie s a v e r a g e 3000 m g./ Kg. We h a ve a ls o fo u n d t h a t t h e t o x i c i t y i s lo w e r e d b y th e a d d it io n o f so d iu m 1s u lp h a t e to t h e b a r iu m fr e e com p oun d . The ED5 0 i s o f t h e o r d e r 300 t o 500 m g./ Kg. when te s te d a g a in s t mapharsen p o is o n in g in m ice . HE 0019943 N20059