Document 8RMKnR5kMgVw4rGva11nNOV6e

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE ldenfity:A mixturecontainingperfluorooetanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component ofFC-203CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productonlotnumber was 519. The testsample isFC203CE (2/21/89)C.urrentinformatioinndicateistisa mixtureof 1.84% PFOS, 30% diethyleneglycolbutylether,60.23% water,2.56% Sultone foarner,3.1% sodium octylsulfate2,.1% polyoxyethylenemonooetylphenyl ether,0.12% sodium laurylsulfatea,nd 0.05% tolyltdazole. The followingsummary appliesto a mixture with incompletely characterizeccloncentrationsof impudties. Data may not accurately mflectthe toxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute statircenewal GLP: No Year completed: 1989 Species: Daphniamagna Supplier:In-houseculturesI.nitibarloodstockfromAquaticBioSystems, Inc,FortCollinsC,O. Analyticalmonitoring: Temperature,pH, and DO Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethod: Not given. Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry: Temperature: 20 OC D.O.: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and testsolution preparation: The testmaterialwas added directly tothe dilutiownater on a weight/volurnbeasis. Concentration dosing rate:Daily Exposure vessels: 250 mL Pyrex glassbeakers containing200 mL oftest solutionand a water depth of6 cm. Number of replicates:2 Number of daphnids per replicate:10 Number of concentrations: one plusa blankcontrol Water chemistry during the study: pH range (0-48hours): 8.0- 8.2(controelxposure) 7.9- 8.1(1000mg/L exposure) Temperature range (0-48 hours): 21 *C Dissolved oxygen range (0-48 hours): 8.5 - 9.6 mg/L (controlexposure) 6.8 - 9.6 mg/L (1000 mg/L exposure RESULTS Nominal concentrations: Bk controland 1000 rng/L Element values: 48-hour EC50 = >1,000 mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined to be >1000 rng/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking= 2. Testingmeets the criterifaorquality testing.However, the studylacksinformationon purityofthe testsubstance and actualmeasurements ofthe amount of fluorochemicalproportioninthe solution. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number G 1358-1, 1989. OTHER Last changed: 6/26/00 LR EWIRO.4MENTAL LABORATORY DAPHNIA TOXICITY DATA SHEER TEST TYPE 1I,VOBHCAL TE HI@@Z5 6E@ z, T ORGANI%I--,AV@am-@ SOURCE Ie )@i@@e@aDATE REC'ID1411Z,01& AGE OF TEST ORGANI94S: t24 hrs - old instars (neonates).NO. ORGANISMS PER TREiklMe;L- 14 R TEST VESSEL: 250 ml-Pv-rex glass beaker TEST SOLUTI(14 VOLUME: 200 ml WATER DEMI: 6 TDM STARTEL--Li',i@., DATE STARTED_.fhi/Ag _ DATE CCTJPL= ,SIA@5M2 DILAJTIONWATER Carbon-filtered well Water (Temp. .21) OC D.O. 4?. my/L. pH VI) ,ANALysr-:@@/f E@@(4 JECSO(95%ConfidenceLimits) >/0000(.* Test Vesse No. Initial Total Conc. mg/L 7Amoun ota' Adddeedd] n4a 24 Hours 48 Hours Total No. pH 'D.O. Total No PH Dead mg/L Dead D.O. mg/L Control 0- 0 ELL loc)o 1.11 ooo 2-OD r) Io.X Test Temp. OC Initials & Date Ccmwnts:. 4%q IAA I l@Qutri L4AIstri"e U@@4-kul:f+ei-i-m',,Uqko4 e-4-lryy)vukll Test methodology conforms with the-following two test protocols: (1) OECD 1202 "Daphnia sp,, Acute Imobilization Test and Reproduction Test". (2) US-EPA #BG-1 "Daphnid Acute Toxicity Test".