Document 8O476LQ1qyOzzXnL3DLKdyEn5
NITRIFICATION INHIBITION
TEST SUBSTANCE
Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, oras a component of FC-203CF-X or FC-203CF. (I-Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 501. The testsample isFC203CF-X. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoamer, 3% sodium octylsulfate,1% tdethanolamine,2% sulfonatedalkylethersalts,and 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectinhibitionby the fluorochemical component of the testsample.
METHOD--
Method: ISO/DIS 9509 ISO NitrificatiIonnhibitioTnest Test type: Microbialinhibition GLP: No Year Completed: 1995 Analyticalmonitoring: Oxidationofammonia, pH and temperature. Statisticamlethods: Not given. Test organism source: Activatedsludgemixed liquocrollectefdrom the Metro Wastewater Treatment Plant,St.Paul,MN (Prewashed and adjusted priorto use inthetest). Test condition:
Dilutionwater: MilliporMeiili_QTwMater Mineral medium: Per method. Reference and testsolutionpreparation: A stocksolutionofthe referencesubstance,1-allyi-2-thiourweaas, prepared by dissolving 116 mg in100 mL of Milli_QTwMater. Test substance was added directltyo testvessels. Test vessels: 500 mL glassEdenmeyer flaskswitha totaltestvolume of250 mL. Number of concentrations: fourplus I referencesubstance and blank control Temperature: 19.8- 20.6 OC Total suspended solids and pH on day of testing: InitiaTlSS 4.98 g/L which was adjustedto2.97 g/L. TSS inthe finaltestsolutionwas 1.49 g/L. InitiaplH 7.9. Blank controlat0-hr.pH 8.1 and at4-hr.8.5.. Element Basis: Reduction innitrification.
RESULTS
Nominal concentrations: Blankcontrol8,2, 118,252, and 496 mg/L
Element values: 4-hour EC5o = >496 mg/L
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conductedon themixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone.
CONCLUSIONS
The testsubstance4-hourEC50 forISO NitrificatIinohnibitiotnestwas >496 mg/L,thehighestconcentratiotnested.This concentratiodnidnothave an impacton nitrifyionrgganismsfrom activatedsludge.
Submitter: 3M Company, EnvironmentalLaboratoryP,.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:ilmischranking2. Testingmeets allcriterifaorqualittyesting. However, the sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution.
REFERENCES
This studywas conductedby the 3M EnvironmentalLaboratory,St Paul,MN, Lab Request number N2977,1995.
OTHER
Last changed: 6/28/00
Type Test
ENVIRONMENTAL LABORATORY ACTIVATED SWDGE MIXED UQUOR
11.
R
LDate
Analyst Page I of
q
ACTIVATED
SLUPGE
MIXED LIQUOR
Date Obtained Mixed Liquor Suspended Solids(MLSS)
Date Used
ilq.g_ g/L
Adjusted to
MLSS in finaltest solution
g/L
InitiaplH
(isttestsoin.prepared)
FinalpH
Comments
7 g/L
(lastsoln.prepared)
TEST SUBSTANCE Sample Description
3C 17- /A
InitiaplH of highestcone. tested
Comments
Adjusted to pH
REFERENCE SUBSTANCE Sample Description IInitiaplH of stock solution Comments
mg/L)
EXPOSURE CONDITIONS
Test Duration
lie
11^11@c t (11f
Test Temperqture Range: 11-k -.)r,.1, *c
Comments
Adjustedto PH A'.n
Sample Description I-C - @t)-@e-r -
EnvironmentalLab - Work Sheet
Lab RequestNo. IV_-@@-l,.I
Page
of
Date:
Analyst:
rr
>i l@j
rinL
@-o t
L "iL<@>,
ENVIRONMENTAL LABORATORY ISO NITRIFICATION INHIBITION TESTINO(ISO/DIS9509)
RAW DATA
TEST SL)BSTANCE-.F("
IX
LR DAT E ANALYST -"4 t3 Pg -3 of
READINGS IN TEST SOLUTION@AT EXPOSURE TIMES - OXIDA'IIONOF AMMONIA
TEST SUBSTANCE CONCENTRATION
mg/L
0-HRS
NH3
Temp.
mg/'L PH oc
EXPOSURE TIME - HOURS
2-HRS
NH3
Temp
mg/L pH OC
3-HR$
NH3
Temg..
mg/L PH OC
A-HRS
NH3
Tomp.
mg/L pH OC
BLANK CONTROL I,,,
3e,
qii
Ilse P,
111,; 5" .?C.(,
q
b
I/CIL,
(.1
7 .4,,1
ISO ACTIYATED SLUDGE NITRIF CATION INHIBITION TEST ISO/DIS 9509
CALCULATION OF RESULTS
LR DATE ANALYST
TEST CONC.
NH3(i) NK3
mg/L
mg/L
EXPOSURE PERIOD
NH3 Inhibiti(o2n ) mg/L
3
Nti3
Inhibition mg/L
Inhibition
3e
qq
77
7,1
C)
17
C)
-3e
ECSO (9$% CONFIDENCE INTERVAL)(3)
( I) REFER TO PG. -@
-
.
(2) PERCENT INHIBITIONOF NITRIFICATION- (@Cto-_CCc) XIOO
- -*jmere7Ct - NH3.mg/L Intestflaskattimet Cc - NH3, mg/L Incontrofllaskattime t Co - NH3. mgll incontrofllaskattime 0
(3) ECFO (95% C.I.)celculatuesding:-
SPECIFIC RATE OF NITRIFICATION (R)
R
=(MCLrSS x4@
Where: Cr - NH3. mg/L 1-inhibite(dATU)flask,at4-hrs
cr - NH3, mg/L lectontrolflaskat4-hrs MLSS - MixedLicuorSuspendedSolids,g/L,Intestflask
romn2t# -PWO
SolidsITS]ITSSIITDS] 3M EnvironmentalLaboratory- Raw D3ta Sheet
Method FteterericeS:id.Methods (14thed.)pp. a%ga, sec.208
9 residue x 1000 Calculationm:g/i Residue - m
mi sample
N Aq 77
Sample Description
A,
Reported Result mgti
mi Swnplo
Gross Weight after Old% c
(Ikkr rU entr@qbfe
Weight (g)
Residue Weight (g)
ol
A.I.
7
':,rL,k
'73 >
2,
0. C) c)Iq7
6 6 -7q
No.
No. "cum
GA CJwk Residue nVn
ICl
Solids[TS][T-SS)[TDS] 3M EnvironmentalLaboratory- Raw Data Sheet
)ds(14thed.)pp.%98, sec.208 ng residue x 1000
mi sample
ded Result mgil
ml Sernple
Gross Weig@t after,-1:3 c
t* Weight (g)
AlIq9 7
"*."Qum
OA ChCk
Residue Wei ght (9)
Residue nVn
C7
Comments
CR.,A5-
&537
1L6.@3
o o.01
C)lq7 601
0.
17.1 tyiiIL
3 3 IL