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TECHNICAL REPORT SUMMARY
70: TECHNICAL COMMUNICATIONS CENTER -- 201-2CN
important =Hfmportpeintedcnbotsidesofape,mdtwcopes 9TCC.)
Environmental Laboratory
Fate of Pluorochemical Project
Aquatic Toxicity Studies: Fu 3925
D. 1. Bacon A. N. Welter
(Fan. 8, 1079
0535
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018
00563
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sTFouOnFDfdieostheprme(iLenmpeoamtiahsemeaxaocprouoctskeeidr9usto)haovnuadrryLitnhCg8e04c8voanhlcoueeunrtfrLoCagr,tfbnlevueagloiuflel
T(oLEaxnbtv)oiirtoynmental TM* 3a2s.
) Lapatis
REPORTABSTRACT: (200-260 ee Sl el words)Thissbetrectinforms Sroionumdd|oy Tie Cancer over 0
Tdohteinliitmiivteeddewtaetrerminsaoltuiboinliotfytohfe FsUqus3t9i2c5 ptrooxhiciibtiyteodfa
t1hCiqs, Vteasltuemsateexrcieaeld. theBowtahtevrerstoelburbaitleitayndofinFveMrt3e02b5r.ate + .
sei pR..A. "PRriockkoepr
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CONCLUSION
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Tdheeterlmiimniatteidonwatoferthseoluabqiulaittiyc otfoxiFcii.3t9y25ofprtohihsibittesetd maatdeerfiianli.tive
sBooltuhbivleirttyebroafteFian3d925i.nvertebrate LCgo values exceed the water
INTRODUCTION
pTehrefotersmtedsounbsttahnicsemaFtMer3i9a2l5 eisvalanuaotfefd-wihtistesqsuoaltiidc. toTxeisctisty and the data obtained form the basis of this report.
METHODS
A. Aquatic Testing,
mTohdeelteedst apfrtoetroctohlast udteislcirziebdedfboyr UtSh/esEePAst(u1d8i75e)s. were
-
B. Acclimation Procedure
Tfhreomblauepgriilvlatesunhtaitschher(yLtep.omisStmoaccrkocfhiirsuhs)wewreree hoebldtaiinneda
wfeilblerwglaatsesr hatold14i-n1g5gCa.nk Afidllaeidlywipthhotocpaerrbioond-foiflte1r6edhours
)
p1iegrhitodawndas8mahiounrtsaidnaerdktwhirtohughaou3t0-mtihneutaecctlriamnasfiitoinonand
ftoeosdtibngeipnegriwoidt.hhelThde48fihshouwresreprifoedr Tteotraan-dMitnhroduagihloyu,t
pthreiortestto pteersitoidn.g. Fish were acclimated for 14 days
v
:
oAnn FacMut3e925s.horCta-rtberomn-f(i9l6t-ehoruerd)wesltlatiwcatbeiroaosfsakynowwans cpoemrpfoosirtmieodn
:3
waaqsuaursiead (a3s5xt2h0ex20dilcume)ntcon(tAatitnaicnhgmen1t6 1l)i.tersAllofgldaislsuent
Towrendtiyluebnltuegpilluls stuonxfiicsahn,t uconmipfroirsmedinasiszteu,dywecrheambteers.ted
aatlleoaccahtedchetmoiceaalchctoensctentcrhaatmiboenr. witThesitn 3f0ismhinwuetreesrafnodlolholwying
tcooxnidcuacnttedaadtdi2t0i-2o1n%. CB.luegMiolrltalsiutnyf,ishtemepxepreartiumreen,tsdwiesrseolved
oaxtyg2e4n-holuervelintaenrdvaplHsoofraullntitlesttotsaollumtoirotnasliwteyrehmaedasouccruerdred.
Gweenreeraslimoiblsaerrlvyatrieocnosrderdelwahteinve atpoprboephriaavtieo.ral changes
uThseesdeinstutdhiiess swteurdey wpeerreforcmoendsidinererdepltoicbatee.genOerrgalalnyisms healthy and free of disease.
AThperilDap1h9n,ia1m9a7g8nafursoemdthien MtohgisulstCourdpy. weTreest opbrtoationceodlonused was that described by USEPA (1975).
+ Dale Fattig Fish Hatchery, Brady, Nebraska Commercial Fish food of known composition
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pr
-3-
i RESULTS hTahde banlueagvierlalgesuwnefiigshht (Loefpo0w.l5smgicraonodhilreuns)gtuhseodf 4i.n0tchmiss. study T0h.e63lgo/a1d,ingpejfoawcttohre fmoraxtihmeusme rsetcuodmimeesndewads loaapdprionxgimfaatcetloyr, oPfH 0v.a8rieg/d11t1r).o"m 7."8Tes-t.8.t2emppHeruantiutrsesarnadngoexdygefnrosmat1u8ra-ti2o0nC, at 96 hours in the individual experiments were 34 and 56%.
sIinnctehistherseeplivcaaltueeds swtouudlyd, eLCxs)cdvatlheuewsawteerresonloutbiclailtcyullaitmeidts of FM 3925. (Attachment 2).
T1hg68Da,phpniiamTaaggngae osftu$d1y2wa-s8.c7onadnudcteadmiantimaaltem4p8erhaotuurreoxoyfgen
ssoaltuubrialtiitoyn ooff F79M%.3925P.roje(cAttetdacLhCgmodftv3a)l.ues exceeded the water
TbhyeM.daTt.a Erelpnoarbtaerdavyh-eraenidnL.areH. baPrsietdchoanrds.tudies performed
REFERENCES
1." MUSa/cErPoA-in-vMeerttheobdrsatefsoraAncdutAenphTiobxriicaintsy,TeEsctolowigtihcalFish
)
Research Series 660/3-75-008, 1975.
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TIETRICE Te Sgr Ha #2
! Type Test__Shoow Spat " Veterial Testes TM 202F
tessorpmtsBn leadYuiul) Avi. WLS frameAve. Sized
Test Solution Volume \b Liters/Task No. Organismsperdose.
20
Time Sta2,r10t 0PWeAud te Started _4:1\-]% PeteCompleted <}-\8 S
Dilution NetePiir ter-_WeCll Wyater r(Tebwp1oS sC, 0.0.88pre, p_73 )
InvesWtT,iEglaanatbaomur ny LC 50 (95% Confidence Limits)
hwm ll loge ole jones] 20]0 Rsler]| 0[eafeoll 0Tedaol] 0 [adc]
goed] 20I 0bslesl| [neleall hyleoll 0|nafsor]
[wll ze]o keleall"o Jaleo] 1 [slcell 1 |2s]cr]
(22] 20 Tufeell 0Tovlcall 2 [esl = [ole]
(ze 20] Tuleell 0 Jase] 2 fusca 2 Jogo]
[eel zo] 0hsleell 0Tafel 3[arfeall = [ass]
[roll 20170 Tasfeell 0 Juss] 7[ales 7
[as]co|.
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Test Solution Volums__|[p Liters/Task Wo, Organisms per dose 00
;
Time Started_3.2,0"PW Date Started 4-\FN\__ DateComplorsd__ 4-22-79
Dilution Water_Cazbon Filter - Well Mater (Tewp.\7Css 0.0.89pre. WIR 1
tvestigator_ERudNa\ Lc 50 (95% Confidence Limits) ___
f.. 24 Mrs, ool 2be 0.0.
--
wh ao[o |}.x oosshela y hahal1lTulsd loo11hslesl"a Tolan" > halanll 3Taalsel wl aoI Taglsull 1 usfs.lfl \halsoll 1Teal] w 020|o Rolsall oval] ohalugll 0Teslsol
aeIohalsell o hakull o haaafl 3[24lzd ol mo| oheliall 1 Jeolsoll 1Relall 1 Taslaol a Profsdale ahohells halal o piler)
em We
meats:\) Wegef
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TostOrgants DnSanus oop AVE. _Scgmms Avg. Steeca
Tost Solution Vohuur)m, weanles0BEQE Mo. Organisms por dose_ % 0)
Eden inala ale Tine Started X10Tr: Date Started WWAM\Y Date Coupleced_W-ALNY
Vilution Water Fil_ terC-NaellsMab teron (Tewp.\A C,, 0.0.3)pee, wi3))
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= ht eseflenlo TTH Mahalal TE TI 117
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Comments:
cove oo Sean nenXN Cov a0 Non aS
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Coe TesteTnghuinYaqpa, Material Tited3 EY92 95
TTeestt SoorlgutmiionnDVoolsumiemJ80)WSotagmpmaBE
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Over.pagniar ns poor doAsveg,.
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Timo StartedL06Wwy Dots Started \~ON\-"\ Date Compleced 4-24Y
Dilution Nater_Casbon Filter - ell Water(TempRQC., 0.0.38ppm, #431)
Tin im ala ake] InvestigatorinPecNhard LC 50 (95% Confidence Limits1 ) 30A tvs) Yn B\ueavaey
img. [Exposed | ko.Dead pit pie ||No bead si pom |INepond oh _pem|lveuomed si 3
BallasLol] [Tv Tealeell T0T TT]
Seo
Jo TT MoTleTaTl1aa1l1l1
soflac lo [1 ll ofestzel TTT T7}
rgge]] 20| 2 epee
HH
deol aoJ1211[NalgeTfTa1el1l7]
asell moTaoRelsd[=e]FI TTJ 177
sual ne TaoRufefloolETM T7171
well ao FaohuRsfleolETT 1171
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LR 157
Ru RaWpR1STD.AY3,20MAY 11, 1978 13134431
ASR
ENVIROMENTAL RESESRGH LAD. ~ DAUTH +sPRODIT AWPAYSIS FOR BIDASSWY DATRess
J Game EE NE. CUI:Tokcunve 1 74
I77NP6U1T30000D,,2R20T0.00A4.-CONNGD:.. EXPOSNOE.DDEA,D
77.21430%00,,2200,,2102
28
cLooxs. . FRRaEcTSIOInN
1000
2.812913 o8.2
"CHI=S PREMBILITY 15 > 0.03
Lo
oITEwRATIONS neces suKk ave
6
24.7904. 7.917801
((gI-T3E0RD=SEMIETY
FACTOR OB 1331.882
=
1
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AN
femy \. :$
FFIATCTTEIDON. DDeshpdlhmiad Mahslaue a
00:.910455930885%5"74.
MLILNEsARoIFTYo PcRiOBRsBoTFLImTY
'
1.61078 0.446226
6 = 0.1348014.
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