Document 85BwkbB9bnepDN3LgLvm3Ogxm

A * ` wilari ^ < Q INTERNATIONAL l e a d z in c r e s e a r c h o r g a n iz a t io n " MAO 0>MC0* ***4'*0. Av,*.w, *. -v TO*H IF. *. * o*,o. *o* 6epteid>er 2W, 196V To Hesters of tb LIA-HAPO Health and Safety Ccrasltteei Subject: 1965 Fesearch Budget - Proposals and Work Scatenents for Consideration at the 1X>5 Budget Meeting Attached la the 1965 Catalog of Health end Safety Besearcb for your reeiew in preparation for the Health and Safety Comlttce Budget Meeting to he held October 196s*, at the Lead Industrie* Association'* Conference Boon, l'xth floor, S^/2 Kadi a00 Avenue, Bev Tort, X. I., at 9:15 A. K. This mterlal 1* designed to be used as a guide In our dlacuaslcni and coctalna, besides a list of ltexs to be discussed, review and coeoeots os past performance of acne of the projects. Also attached are copiee of two project prcpoeale to be discussed and voted or. at the oeetlng. There will be ether projects for our consideration vhlch you will receive at the ace ting as they have not yet been submitted to this office. Thla la the reason vfcy the dollar figures In the Catalog are lnccoplete. Please review these liens carefully and be prepared to aake your decisions at our asetlng ao that our recccssendatlens say be conpleted at that tlae. We suggest that you bring the Catalog to the netting to serve as reference. Very truly yours, JtrCTS- ^7 Dco 0.Pooler, Secretary Health and Safety Ccnalttea mysad cc 1 Chemical Subccoslttet Technical Steering Cosarittee toe,1 Catalog LH-75 VU-M LIA11578 \ 4 f- " I > i I t T i i i i ! 1965 CATALOG 0? I7ALT3 ATO EATSTT - llAD LIA-ILT30 EEAtra AKD SATETT OOWtETTEK I. II. III. Review of Exltlr-g Pro^^** aod Extension PropC*l Work Stateoect* Extension Prcpo**L* i r1 .j ii^i .i ,ii..11 ..u.L.1". -f.y.'iie *. --1 -a au. i *,. m&g^i1--yj w *im.io^yjyiPSfWMnu.. N 405.01 1965 Catalog of Health and Safety Research - Lead I. RTVTiV or DCT9TCTQ PFOJTCTS ATO EOTOSICS FT-CPOSAIB LK-75 ~ Kucan Exposure to Particulate lead Cocrpou-i.'-* In the Air Under b;er:astal Cccdltlor.a -- V:,a Lettering Laboratory Progress 00 this project la oc schedule cod wo are asking for a 004 year continuation at thla tine. Cur need* will be satisfied when the Kettering Laboratory has developed guidelines cn the amount of lead that will be aafa In urban atmospheres, which guideline* will be bade available to goveracent agencies upon retreat. Budget Aecosaendatloa $35,000 lfl-86 - Lead Clare* for Dinacrvare - Rutger* University Lifted for Information purpoaea only. The money va* appropriated In 196k. Amount Appropriated $6,977 LB-7$ - The ?ctln- cf OrERC-slcad Crrrr-yund* for Possible Bidocrlaologlcel and Ar.tl-Karaary Tunor Activity - The Ucrcoter foundation With two organclred cceprurd* elreaiy *helrg excellent prcalee In anti-Inflaanatory activity thla project baa cade good progress. Work will cootlnue In further exploration of prosit log cocpcuada that already have been screened together with new compounds to be obtained from the Batlck and TWO labcratorlea. Budget Pecaaseodaticn $ LB-79 * Tcxlclty Testing for Qrganolead Cotrpounda - The Hlne laboratoriest Inc, and Industrial Bio-Teat lalcratorle*, Inc. Listed for lnforaatloo purpoaea ccly. Orgacolead ccmpouada to be tested ooly aa tbs need arista. Bo new funds needed. Money was appropriated in 1S6h. Amount Appropriated $10,000  V vijq *" msy' .wg.*i v\ t",! i LIA11580 1965 Catalog of Health and Safety Beaeareb - Lead n v;?x trrxmc-^rs DLH-lk -- The Radioprotective Effect# of Inorganic Lead (Prepared by the lev fork Medical College) UIS-15 -- Toxicity Study of Ingcated Paint flla (SuLcltted by Raxeltcn Laboratorlea) rn e c t e s s ic s p r o p o s a l s LH-T5 -- Bvrsan Expoeure to Particulate Lead CoepcuDda In the Air Cbder Experimental Candltlcca -- The Kettering Laboratory Lfl.76 -- The Teatlng of Organolead Cocpounde for Poe tibia Endocrinological aad Antl-Mnrceiry TNaaor Activity -- The Mcrceater Foundation "srer LIA11561 G PART X PROJECTS PCH CCSJEEPATICSt AT THE LEAD HEALTH AJO 6AJVTK COKCTm 19<55 HUDGET KDiTD*3 Project Title eod Cootr*ctor fundi Peelilea ______________________________ ____________ .. . Feoueited (Approved Tor Axalnat LH-9^ A Biochemical Approach to Dead Toxicity -Barrard Medical School \ TOTAL i W5W "iiSj-'i1 iBW ig*?''1 y11 ' N 405.02 WS'TygBl >*yjWlft gi't>U" ; B. LIA11582 PART II WORK OTATOOTO FOR COSSIDERATIO0 AT TH* LEAD EEALTH A*fO SAIT7I COfrOTTEI 1965 BUDGET KELTISO Work Stateoent Tltl* fund* Re?je*te4 Approved UUB-lk ft* Radloprotectlr* Effect* of Inorganic Lead 10,05k ULa-15 Toxicity 8to<Sy of Inge ted Paint Ella $ D*cl*lco Agalnat I !I TOTAL ' .-; 1 % ' '-' iJ-:^',-m`^!: T*. L IA11583 1 1 0 PART in EXTiSSIOfl rPCKCALS FCH CCNSIDEPATICS AT THS LEAD HEALTH AID fATETY COWXTTEX 1565 b u x e i KErrrw Project Title and Contractor fund* Dec1*loo Approved 1 For t Ax&lnet lA-75 Buaao Expoaure to Particulate Lead Cospou&de In the Air Ubder Experimental Condition* -- Ketterln* Laboratory 35,000 IH-76 The Teetln* of OrganoLead Ccepouni* for Poealble Endocrinological end ABtl-Hasrwry Taaor Activity -- The Vorceeter fouodatlon $ TOTAL j i ;; yaw.-TMs*a4n; * f.<r nuw; w^sseg^Ti j!UJww wvj^ jW, a^igiyw^ L IA1158 4 ------ . Till* of Prcjecti ULB-lV THJ PADICPROTECTIVE ETT8CT3 OP IR0K1AHIC LEAD SPirrnc a d c ; Chenics.1 egent* utlilted la the radiation protection of tissues, organ* and uhol* aalanls vary * to structure, code of action and spec lee etudled. Trace netals play a vital role at Intracellular co-factore. Zinc, cobalt, Iron, ongneslta, nansaneee and copper an . eaae of the cetelllc loce vlth established function* In various enzyse and traneport systex*.^ Ret veil understood le the high concentration of cadnlua In the renal cortex and the relationship of selenW to cuacls oetabollsa. It le conceivable that In the cocpiex, Inter-related systex* that control cell function, radiation Induce* rapid lonlxatioo, foraatloo of free radical* and dlerupt* nolecular epetlal arranserente. In such situation* the varloua Bctalllc Iocs vould undergo severe alteratloce In their valence shell*. The possible introduction of appropriate lcc* to stabilise the carl/ changes produced by radiation Is vcrthy of stud/. This laboratory has an unusual cocpetence In cytocheaistry and hlstochenlstry. In addition, a tajor group of Investigator* are studying the structure sod function of the cardiovascular eye tea. These Include1 field Formerly Dr. Sara Schiller Blocbexletry (Silver* ity of Chicago Dr. Villiaa Gutstein Biophysics Rev York University Dr. Ralph 6trebel Exp. Path. Institute of Research, University of Montreal ' (Dr. 5*lye) Dr. 8. Mchoc Exp. Path. Dcvnstate Medical Center, I.Y. Harold Appleton, M.8 Clinical Rational Institute of Bealth Chealstry Dr. Bernard Vagner Exp. Path. University of Washington Working vlth this group ou a part-tlas basis Is an electronic* expert :- sod s physicist. The tissues frca the anlaals la the various experlaental group* vill be prepared by special techniques developed lo this laboratory to evaluate Intracellular, extracellular sod vascular changes. Vas cular changes Include fixation for observations of constriction or dilatstlon. J-* Frsllclnary studies employing Inorganic, ionised lead la th* Intact anteal has indicated that the vascular chauge* consequent to lethal vtiole-body X-irradlatloa eey be aodlfled. The purpose of this Investigation is to explore in detail the following question*.! 1. RadialUn projectlco of lead as a function of does, tin* and i tissue levels. S. Influence of saiaal age, sex sad diet. {. Mechanise* of protection at s hlatocbealcal, cytological level. . Blood sod urine charge* during experlnsnt* yej.wai_iy1i ****!!**. N 405.03 ! Ill IUIWIJIIl -till') w'aww^wwiwroaaFiaiwgt^^ LIA11585 .-`ifc. -2- METHODS: Experiment I Validity of Prevlcut tt>#rratloce Oroup A ZB Pb(Ae)2 BaCi 200 ag/kg 200 eg/kg 6 rate 6 rata bOO r bOO r XC Fb(Ac)2 n> R>(Ac )2 200 ag/kg 250 ag/kg 6 rate 6 rate bOO r boo r XX R>(Ac )2 250 ag/kg 6 r*f bOO r XT a j *03 za AgSOj 200 ngAg 250 ag/kg 6 rate 6 rate bOO r bOO r Jot#: Doeee 50 ^/kg/dy X-ray: 24 hr#. after latt dot# except 6roup# IC and IE where lrredlatloo precede# talt adalnlttratloo. Croup ZB It a ebaa treated rdl*tlco expoeuxe coctroi. VI#tar rata 100 gat. ETTERIXarr XI ty - - * 2A (6 23 (6 2C (6 20 2X (6 22 (6 4 4 Effect of Eoee ftod Tlae g- -3- > ____2L____ L - 4 4 4x 44 -4 *4 -- X 4 * X 10 . X 4 4 4 lb 21 . X 4X - 50 a* n (a c Jj l.f. lo ln>#CtlCO II bOO r X-r*y The txperlneotel do#ign It to relate dot# ecbedule u * fixed variable to tlat. la each croup of 6 aclBalt tber# art 3 celt# tad 3 f#aal*e of 100 ga Initial wight. Sot# th*t Oroup 2B tad 22 vlll rectlv* H> la th# poet-Irradiation ptrlod. Experlwot in Enhanced Diet Study Oroup R#p*t of Experiment I vlth th# following exception# III-IA Ad llbltiaa dlt III-ID &>rl<--h*4prut#la dl#t 255 Including M anloo told# III-I2 Ad lit IXI-XO Enriched Th# CH rich eaiao eclde till be tdtlnltt#red by (ravage tod vlll cccatltute t 255 Inc. la protU) M owr tb# etandard dally ccoeueptlos Of food. i,, wwuw. jtjg yr>,,-. ivjy i.'myfi .a V.****^' LIA11586 mip p s w mr i MAJjJiyi -3 Throughout *11 experiments, blood fcnd urine samples vlll be obtained t Judlcloj interval* for the following date ruin*tlocsi BLOOD Hemoglobin Hematocrit Perlit.ers.1 VBC sod differential Ea,K,C*, alectropberogmB Lead i 01 U3IRB lead Protein# Cent* Cells, Bp. 0rarity Tissue* obtained *t autopsy vlll Include! Emin, Lungs, Heart, Liver, Spleen, Stoeach, Kidney*, Adrenal*, Gonad*, Shin, Bone, Bcoe Harrtw. In addition to routine staining, vfcer* Indicated tbs following hlstccfcenical reactlcc* vlll be acAlyted. 1. Oxidative eosyats 2. Phosphatase* 3. Laxlc protein# a. Connective tlxroe polysaccharide* The experiment* vlll be modified during the course of the lnvsstigat loo *t the discretion of the Investigator*. Proolelng leads vlll be followed at no addltlooel expense. The experimental design vlll yield statistically significant Infonset loo vhlcfa vlll ansver In Largs part the original problea listed under title of project. The project vlll require 6-8 nooths for eccpletloo. CURRICULUM VTTA1 be man! H. Vagner, X. D. Ralph 81rebel, Ih.D. Lillian Bedell, BJt. - Professor and Cbxlrnan, Dept. of Pathology (Prime. Invest.) Assistant Professor of Experimental Pathology Chief, Histochemistry Laboratory PACTUTna AVATLABUt Animal Facilities ' Histochemistry Laboratory Cytochemistry Laboratory Htstopathology Laboratory X-ray machine (supervoltag* available) RF7TTICTCK3I I. Pntyaest Dlsoo, K. and V#M>, B.C., Aeadaalc Pr# Ino., Rev Torlv 195 8. Schubert, J.R., Muth, O.H., Oldfield, J.I. and Raaaert, L.f.i Experimental Result* vlth Celeolvx* in Vhlte Musols Disease of L*nh* end Calves, Fed. Proe., POt 609, 1#>1 J. Vagner, B.M.i Otudls* of Vasoconstriction, Dull, R.V. Aoadeegr of Mrdlolne, 381 66, 196* A. Van Cittern, R., Vagner, S. M. and Ruxhmer, R.l Architecture of Small Arterlss during Vasoconstriction, Clro, Ns., IOi 668, 1968. s i . s. , :' LIAl 1587 T Jl. o 5. Vfcn Cltttn, B., V*grir, B.M. *rx1 RukhsMr, R.t Artrl*l Vail* during VaaocooJtrlctloa, Cor et Vts*, li**, 1962 (tort in Rufttl&n) 6. Vi^Mr, B.M.t Alrutl&n PlictH of Mink, Arthritli tod R>\*llia, Au^uit, I963 5 ft ifi I 1 ' LI AE&5.88 p'I''1 |jy -5- PTKXSWTL puwgr Principal Icvtetl gator - Dr. 8. Vagnar Co-Inveetlgtor - Dr. Falpfc Ctrabal Technician, part-tlot Animal Ca t *-taiar, part-tin* cyrnxsff sen CCS31HA3L3 SUFFIXES Aclnala - Vlatar Bata (100) - Animal food, bedding Hlatocheaical raagaate, auppllaa Hlatology aupplla* Blood and Urloa taata OTKFR TXVn&TS X-ray technician, pert-t la* and ua* of <julpnt TOTAL DIKBCT ZXFBtSZS Overhead 10* *9,1*0 9U* 3,000 2,500 l,tt0 200 1,000 300 500 200 *10,05* TOTAL -< !-. .w. .ruij'ya `^ii-wwvi jaw, ig'j_.gj abjn'#>?!y?ay- mu j p,wgi'yi!^jej-'piy-" "V''1'