Document 82nnY3LJXdegRdZEpYDQN8zpy

*> ;>'i Sr , *.. * . rV. ? k r/iH REQUEST FOR APPROVAL TO RELEASE PROPRIETARY TECHNICAL INFORMATION 4 f I 1 Alio* `+i.*vn I. O'-- ttv. Ipf"! lin<1 ft i bj*< iJ *<*#h tu* * + | ' . Ml Mjr| . ^Gb-AlSSjU. *n > tlc 0' manuscript: vl:;vL CHLORXDL METABOLISM AND TUI: STIMULATION OF j JM'LENIC LYMI'IIOtYTK TKANSFORMATIOM IN MICK ALJTmCR En iB i r Aft .. vsa rcu j ITai 0f AfliTJ'AC! I r-j I i)(i I *i1 j i') fit a *C;^ p( ^ u?iP'PCHTt ) ro:*,L_ ;/ firLw3ifiC . .J.____ liiur. j___ ' "STl'V-A -iV-i s'r"d op r a: ........... I p!0-e. c,. asI'ET - 1978, Houston, Tux as or lUI'HAK - 1978, Paris, France Los . . ill - i - !'*. -fj. r RELATED PATENTS OR DISCLOSURES RELATE!- PRODUCTS ON PROCESSES RELATED REPORTS ON PUBLICATIONS fi r\ fV. Nona ;....... ...... Vinyl Chloride Li. .preparation................... 9v 1 * i" 31- O 53 I SUGGESTIONS BV AUTHOR EOH POSSIBLE RVir-FKS OF THIS MANUSCRIPT I ov (.fOAl a*. J'LCTS '"'l.lf* pA, t* -T*Cft I I it V 1 I 0I.` li`; ,ini a0f f C ; ' l *> I * ,''r s : , ' ' , * ` * '* ,, j n ' r f CP UCnicaL aspects 'Inrlui" ! n^nr.'ir-ifp'ol > "> E I * IN ,'f l(Vo /.*Af*As^f,Mf!NT 'a T r >. * CjL pa ST Mr N T ;KA'.! V.-.RF MCCN .PAT! Nil i.LSAl, DEPARTMENT P-L" (3 >'c:a,'.: n. par c- nt n.-.mi JloMp Farher . /_____ ! dlP'. Inorganic Clnr.iicnls i G rn. I I I- 1 NAM! __ _ I i rf pt. "" ' ' .. ________ _________ | [3 OlHft- j Hami Earl Smith ;.i i-r. Ijior[;.mic Cht-cii cal:; JT] Hf ALIH f. i :iA!RONMt'!.TAL Mrs. ' name _yR_owc________________ f ' t. Toxicology Research (~) 'roiNOLOor center name _ ____________ E> E p T. _______________________ o:h[p nam! .1, .Iorp.eluon___ ;;rn;. Cot J)OTU LcMoiJi C.'l j 33 fi (/> to ! name: .. : r. p. 1801 QUESTIONS CONfENNING TE'lN nanUSCNiPT aNE TO HE DIRECTED TO o Shanr.a .. cn "l ''T-l!i-aJ th & Environmental Resi',lYc?i; 618-0251 __ IO CA T i T'-A I r C!T>: Midland MJHMI IT ti- 1 G-- 7 F.t [ C f T':............. ................. 2| Cpnipie'o S'*. 11 oil ?. Scmic `lp|.Mi|ii mle u,.p.|r"ni?M'T .if,p I -.T-I ...I.:.- Id CENTRAL KCPC'RT index: iliutr. N. '>. S ' U t r f* * t *hi, fox H .<* sr i DEPARTMENTAL approval FOR RELEASE I i*3.* r * rj j "ns Hiiiustntil 'i'M j .. i * r , * Eli -"_'^*i P ?, rfVj.E* < Thf Duw > firif .K--- j WMAr'-t' / F.tAfJAf,1 V. ri"ri`rLF7 da t;: ,77 C'1/ $ 7 7 'frfw l.E sal J'.P! C *' : !' fiUSirM"'-. _A.s Ti "> ` ' T Ev'^n'*.-. Al ; A>rr Dr:FARTAieNTAjA'5eL"fCTtON OF KcVIEltKS IS-- illiEifi sug,;',i,,E`ri M/: r feppj?.ovF5 j ] rott rL'Li;c,mic: i rVitC-OW vHTT oi Scctton 3. Scon the dftoc^<f<i moKuitnp1 *J nm l fi urn *. n 1S t 5 (, f)tnrinr: v#c ^cnv> Jt'tesl l)v tJ*c RpifflKh Ditrctor mm^ti l.*- v onr4 titi.il tm irlt-fisr wilt t-r 91 .*n ; ^3 .tnnviivr.pl to CUiT.^AU.WFPOR T irun, x. `iUllDlNu vo. MlOl AND. ______ Z.: i os.' wiJ! h jnti tl*i v ^CiFm : .:vMt:; ftPPRCVro fO" IMi;u:CA: :t1'll.^v oF :" W UICI:0mm[' Nr1A i IQNS 1 Rf LUM LI uritas! ar7rR changes ; /o N'l! RE L LAS'. 4: 1 '* 1 t* fj.n 4 -*0*v; .** * ' ttl V iU! % ') *'. *m 'f C" !* *>* t :K,,-i - M-r-LAtt'* J"lf f- 1 if s: c r. I v yr o .7*V( L VI Fl 1-J'j C-- i AA-T- [<}, ; r y. | i a'.E G appro.'!'' a 1 IL R CanCCS -.01 aPpb ;..;s <'T. - > *:C" /'/. E t... - ... aN&r' vinyl anuuniK mhtahoi, icm and the stimulation o: splenic lymphocyte :ransF' xmation in mice K. I'. Sharma and P. ,1. C.'bring The Dow Chemical Company Midland, Michigan 4C6AO il.S.A. We have previously reported that the exposure of aloe to vinyl chloride (VC) caused an increase in lymphocyte transformation in splenic cultures and also Increased the responsiveness of these lymphocytes to pliytomitogens. We investigated the role of VC metabolism in these phenomena. Incubation of mice splenic cultures In .; VC-atmosphere (1000 ppm) failed to produce any effect. A metabolite of VC, Liiiodiglycollc acid, pro duced effects similar to VC, exposure in vivo uni also Increased lymphocyte transformation when added to splenic cultures in vitro. Another metabolite of VC, M-acety1-S-(hydroxvethyl)eyateine, did noL stimulate lymphocyte transformation after in vitro addition. Simultaneous adm inis*'rat ion of ethanol or phenoharbital with VC decreased this stimulating property. PnejiOb.ubit.il nay have produced its of feet either by increasing the hepatic binding of reacLive VC metabolite, thus reducing its effective concen tration, or by Increasing the metabolism of VC through a different pathway. Ethanol has been a known inhibitor of VC metabolism. The results sug gested that VC was metabolized to products responsible for increased mitotic activity in splenic lymphocytes. In view of the previous reports that vinyl chloride disease cay be an immunological disorder, the evalu ation of peripheral lymphocytes in VC-ex;used workers is suggested. R&S 021053