Document 82Bm0zZz5jEdrgJYNeRENydgo

T ?jsjli-ikii4', i ' 1 ! ' ;V(i| . ` ` ' .TOV Inhalation Approximate Lethal Concentration (ALO o f Haskell Laboratory Report No. 802-85 Ee I . dir Pont de Nemours and Company Haskell Laboratory fo r Toxicology and In d u strial Medicine P. 0. Box 50, Elkton RoadNewark, Delaware 19714 Date Issued: January 6, 1986: Company Sanitized. Does not contain TSCA CBf q r _ _ l-r / t M e ^ teuiL fyrlt UWet het0consd1*i0t5ion*saotmf otshpihseres S " ' ' f f l i f & i S ' M i ' ! ' the highest than s lig h tly to x ic on an acute inhalation b a s is / worse Robert, C i g ' Technician / /{ffe Reviewed by: rnofflas A, Kegeiman Technician / 3 f*c~ Stuey D irector: ^OhAJiAnCa ft Laura A, Kinney* Chemist 1 / 3 / L Approved by: ------- -------------------------------------- i / 6 Section Superv1s o r, ' Acute In v estig atio n s Section LAK:smir:HLR17.17 V - 2- Company Sanitized. O oe,,,,,,cmlaln TSCACB( Sgonsor: T ex tile Fibers Department E. * dit: Pont de Nemours and Company Wilmington, Delaware M aterial Submitted 3v: j E. I . du Pont de Nemours and Company Seafo rd , Diaware . Jest Facility: ; E. I , du Pont de Nemours and Company ; Haskell lab o rato ry fo r Toxicology and , In d u strial Medicine ; P. 0 . Box 50, ETkton Road j Newark, Delaware 19714 Study I n itia te d - Completed: 12/7/84 - 12/26/84 There are 7 pages in th is rep o rt. D istribution; - 3- Company Sanitized. Does not contain TSCA CBi UffMDUEriffll jM Hie pl pose pf- ;i$| m4four inhalation SIC for ____ ___ lowest atmospheric c o n c e n t r e * ! * t e s t e d i # iio r c rats either on the day of exposure or withlii 14 #ye;^OSt p$5W*. #cept as documented in the study records, this stidy was inducted ciertlag io the applicable Good laboratory Practice R elation*, tone < # ! deviations noted affected the validity of this study; ' ' T v MATERIALS AND METHODS A. Animal Husbandry i; Young adult male Crl :CD*(SD)BR ra ts were received from Charles R iver Breeding la b o ra to rie s , Kingston, New York. Each r a t was assigned a unique 6 -d ig it id e n tific a tio n number which corresponded to a numbered card a ffix e d to the cage. Hats were quarantined fo r one week p rio r t c te s tin g , and were weighed land observed twice during the quarantine p erio d . During the t e s t , ra ts were housed in p a irs in 8" x 14* x 8" suspended, s ta in le s s stee l;, wire-mesh cages, the ra t assigned th e lower number in each cage was id e n tifie d by a slash in the rig h t e a r. P rio r to exposure, r a t s ' t a i l s and (cage cards were color-coded with w ater- insoluble markers so th a t \individual ra ts could be id en tifie d a f te r exposure. Except during exposure, Purina C e rtifie d Rodent Chow* 15002 and water were a v a ila b le ad libitum . B. Exposure Protocol Groups of 6 r a ts , 8 weeks old and weighing betwe =n 227 and 23 were re s tra in e d in p e rfo ra te d , s ta in le s s steel c y lirJ e r s with conical nose p ieces. Each group was exposed nose-ontjyfor * s in g le , 4-hrv- period to an aerosol atmosphere air . ^ weighed p rio r to exposure, and were o b s e n f c ^ o ^ J r n T c a l s </ns of to x ic ity during exposure. For 2 exjposures, surviving ra te ,,re weighed and observed d a ily fo r 14 daysr post exposure, weekends and holidays excluded except when warranted by the r a ts ' condition. For 2 exposures, ra ts were weighed and observed on the 15th or 16th day of recovery because the I4th day f e ll on a holiday. C. Test M aterial P h y sic a l Form: Tan l i q u id Company Sanitized. Coes not contain TSCA CBi Purity: Composition: Contaminants; Synonyms : S ta b ility : Tli was a s s o n i to be s ta b le throughout the exposure phase of th t e s t . 0. Atmosphere G eneration j Aerosol atmospheres o ^ W B jlU ^ f w e r e generated by pimping the liquid t e s t m aterial to a Wirayfftg Systems nebuiiizer. Air introduced a t the nebulizer aerosolized tjte t e s t m aterial and swept the aerosol stream int o the 38-l i t e r g la ss exposure chamber. The atmospheric concentration o f W P M M w s c o n tro lle d by varying the liq u id and a i r flow r a te s . ThdThamber exhaust was drawh through a cold tr a p and a MSA c a rtrid g e f i l t e r p rio r to being discharged into the hood. E. A nalytical ( The atmospheric concentration o f / ^ M | | w a s determined a t approximately 30-minute in te rv a ls durTffg each exposure by g ravim etric a n a ly sis. C alibrated volumes o f chan&er atmosphere were drawn from th e r a t s ' breathing zone throughprewelgbed Gelman* g lass fib e r f i l t e r s . F ilte rs were dried overnight fib a d esiccato r cohtalnfng D rier! te * to remove resid u al water from th e sample. F ilte r s were weighed on a Cahn ^^Model 26 automatic EJectrobaance*. The atmospheric concentration o f was calcu lated from th e d ifferen c e between the p re ^arra post-sampling weights o f,th e d ried f i l t e r s . P a rtic le s iz e (mass median aerodynamic diam eter and percent . re sp ira b le) was determined with a S ie rra Series 210 cascade impactor during each exposure. : During each exposure, chamber tem perature was measured with a mercury thermometer and r e la tiv e humidity was measured with a Bendix Model 566 psychrometer. Oaring most exposures, chamber oxygen content was measured with a B1Marine Model 2?5 oxygen analyzer. - 5- c omPany s a nHi * * iD o e s oiconiam A* E*Psure Conditions and Associated M ortality s ssp wc^ srser,nt^ a2nd7S2EJ2rS^Sit2r.^*^1;'.re,at,ve Characterization o f g M W ^ A ta ro so h er^ ' and t e f e a t e d Rat- to rt il' y D Particulate* Concentration (nafm3 \ N~ean S.. tZZqe A11f 350 710 1800 iifn 0 - 40 100 250 - 550 64 620 - 790 150 1600 - 2000 Respirable ! i 96 ! 95 81 HffllupQ* ' /*ft-*nVft s2.4~ 2 .2 4 .4 M ortality (# deaths/# exposed! 0/5 0/6 0/6 0/6 b e c a u V ^ 'iS m th l SlneStim " , TM . .r * ,t0 M9h*r " ` e n tra tio n s ere attempted P Sl " " K a 't , ! t ' an W a c h e d the lim its o f B. C linical Observations -H-Under th Dspirrint gSnior irnjpnediate'ly fol?l?owraintgs edxiepdosuArlel,owraintsg einxpaolslure - 6- '^A3o y a not cnt,sin TsC/ OBi groups had a red mg/nr; rats* faces *i rats exposed to 350 exposure* gj^Sgg Z ^ v s ^ s m . & sf i t ! ~ L soe t variTM > ^ W ^ m S u 7 t i, lis in a n f f fur CQSieiOSiQW ') kt bSrra z ^ m ****** ^ "*' ? f ! f U' a5 on e sc r^bed in Sierra Instrum ents, I n c ., B u lletin 7-79-219IH w j g S r l t o r ? !" '" 1 ! M a 210 M bi` " t Ca lL d * IiTOactors and I I Z ^ i: ) 7- Co,,PanySinlffeed. Oe; Snc?confa/n Tsc4 CBf