Document 824xONgbNr2Db7MJG53n5y5ed
AR 226 -- 09/4
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: A mixture containing perfluorooctanesuifonate, which may also be OrceftearnreesdutlofoansicPFacOiSd,) (FCC-A9S5,# 2o7r9a5s-3a9-c3o)m.ponent of FX-3103. (1-
Remarks: The 3M production lot numberwas not noted. The test sample is FX-3103, 3%. Current information indicates itis a mixture of 0.96% PFOS, s2o0d%iudmieotchtyyllesnuelfgaltyec,ol6.bu0t%ylpeotlhyeorx,y5et0h.y5l6e%newamtoenro,oc1t5y%lpehtehnyylleneethgelry,co2l., 54%.32% propane sultone foamer, 0.5% sodium nitrate, 0.06% sodium lauryl sulfate, and 0.1% benzotriazole.
The following summary applies to a mixture with incompletely rcehfalreacctttehreizteod cxoinccoefinttthryaetfilounosorofcihmepmuirciatliecso.mDpaotnaenmtaoyf tnhoet atcecstursaatmepllye.
METHOD
Method: OECD 209
Tost type: Static acute
GLP: No
Year Completed: 1985 Analytical monitoring:
Dissolved
oxygen
concentrations
Statistical methods: Results were determined by calculation of the %
inhibition in respiration rate.
Test organism source: Activated sludge mixed liquor collectedfromthe
Metro Waste Treatment Plant, St. Paul, MN.
Test condition:
Dilution water: Notgiven.
Synthetic sewage: per OECD 209
Reference and test solution preparation: Not given.
Testvessels: Not given.
Number of concentrations: five plus blank control
Temperature: Not given.
Total suspended solids and pH on day of testing: Not given
Element Basis: Respiration inhibition as determined by oxygen
consumption.
RESULTS
-
Nominal concentrations: 2 Blank controls, 100, 180, 320, 560, and 1000 mgiL.
Element values: 30-minute EC50 = >1000 mg/L
002978
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test
Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone. CONCLUSIONS The test substance 3-hour ECs for activated sludge respiration inhibition was. determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. Study lacks description and recordsofthe method followed. The inoculum was not properly characterized. There is no characterizationofthe test substance purity, and the study lacks analytical `confirmationofthe amount of fluorochemical proportion in the solution.
REFERENCES
`This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C1111-1, 1985.
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