Document 7xJZyXaO4nzJ5oO4jyR4y1Do
Wildlife International, Ltd. ae
Project Number 454-107
PFOS: `A FLOW -THROUGH LIFE-CYCLE TOXICITY TEST WITH
"THE SALTWATER MYSID (Mysidopsis bahia)
ADDENDUM
`WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-107 3M LAB REQUEST NO. U2723
SerieUs.S8.5E0n.viErxopnlomgeinctaallEPfrocttecstiToenstAGgueindceylines (OPPTS Number 830.1350
TaFhiesrseteusdynhwaattsthrewarseesrtevpsiusbseeiodnoonvarei77ts1t0sb0uabsensetddaodntnchetrppurouittyrowfraei9sc0ed.tnt4t9pcy%ui.rinTteyhdaent0eysbst.s8u5b.Ts9ht%earneTshueltsSponsor rRHoeirosvoelmypntpraeaassenmtadledadotwaoorAerasaiumccphl,yamciontaoerniatetdoirbcsy tsdtuhaenafdoiarrodooucfnodnticenengwmraoatyipobunresitpoierrsemsaeeanats.ufrtceatdcochooneFcde0n.st9r6ae0ti3ons eaed Samay page om te inl porandth most ect ceocf dntl
EEl g 5 28 2a
Wildlife International, Ltd. 2
Prost Number 454-107
REVISED STUDY SUMMARY BASED ON A PURITY|OR869%
SPONSOR:
SPONSOR'S REPRESENTATIVE:
3M Corporation
SuAs.Baacnh
i
FD"IALNOTACALAATRINEODPNOOARFTCSOTPUYDYO,FTRHAEW. WFiusion, Mearyaiaro2s1,60Li15.0
LITDS. LPREOTIETCTENRUMABTEIRO:N, TEST SUBSTANCE:
----aE -- P(OSeTfRfEvcLrsSuolfionicsAcitdPoitanssieum Sal)
STUDY:
PFOS:A Flow
nLi
Toxicity Testwiththe
opsi bahia)
NCOONMCIENNATLRTAETSITONS:
NegativeSCintrR ol, 0,083,0.16, 033, 0.66, 1.3 and 26 mga. /L.
i
MEAN MEASURED TEST CONCENTRATIONS:
TEA NegitiyeControl,0.055,0.12, 024, 0.53, 12 and 2.5mg ai/L.
TESTDATES:
i
1 (OE-CMaDy 2)6, 1999
ct (EPA-) June 16, 1999
ical Termination =July 25, 1999
`Experimental Termination -- July 25, 1999
LGEoNEvRoATOIFONFERXSPT-OSURE: 35D
"TEST ORGANISM:
`Saltwater Mysid (Mysidopsis bahia)
SOURCE OFTESTORGANISMS: `EWaisltdolinf,eMIanrteymlaatnidona2l1,6L0t1d. Cultures
AGEOFTEST ORGANISMS:
Juveniles <24 hours old
}|
LozEG
hast
TOs mHpaein
036 mgaill.
1
:
|
i
Wildlife International, Ltd. 3
CERTIFICATE OF ANALYSIS
Prec Number 454-107
INTERIM CERTIFICATE OF ANALYSIS Revision 1(9/7/00)
Centre Analytica3lMLParboodrautcotr:iePsFCOOSA,LRoetf2e1r7enc#e: 023-018A Referen#ce: SD-018
Purity: 86.9%
Pw. TopePTE] sew]
[opmrmee =
Identification
|___ WmiE eCEaymlwhou o[y Conforms
|
lo. 1Me1tals (CIaClPo/MiSu)m.
Pea n meeae) ooa oswmk
23.. MSaogdnuemsiom L W E a an ol
| 23 oLooetww a%
45.. NPioctkaeslsium'
E AiR liE
45. 06804091Wwiinn%
6. Tron
me
6. 0.005 wt/wt %
7. Manganes
phe
i
tay
7._<0.001 wt./wt.%
[Tonifolmpunty NMR)|
TT TT
Swim
[adie V7 7 nn ween CMS)
[ce TE Te] (GC/MS)
msm 10 7777 ] en | POAA
Residual
Solvents
(TGA)
Lass
033 wt/wt.%
|"= NoneDooed |
Tnorg1.anicCAhnloiroindse(IC)
2. Fluoride
3. Bromide
4. Nitrate
65.. NPihtorsitpehate
Organ7i._cSAuclifadtse*' (10)
21.. PTFFPAA 43. NHFFPBAA Elemental Analysis
1. Carbon 2. Hydrogen
43.. NSuiltfruorgen
5. Fluorine
T PHEE GE
Hien
ene
alee
ee
Shue
Co a
Bi Si asi
BA
VNr l
a MULE Li
i
Eis
i
% i
Hi EE
iik
1. `Theoretical Value = 17.8%
2 "TheoreticalValue = 0%
34. Triaticall VVaal=Su00%e50
5
etical Val=6u0e%
1 <0.015wLsi%
2.
3.
<00.50940wtwiemntt%%
4. <0.009 weiwt%
65.. <<0.000.6wW0tt/0WL%7%
7.876 wiwt%
21 <<001lwwtmi%% 34002180wwuAmn%%
1. 1248 wt/wt % 2. 0244 wiiwt%
43. 818L47wAtwh/amt%
5. S4lwt/mt%
connzs018A
Page 1013
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-018A DateofLast Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01
"Purity = 100% - (sumofmetal impurities, 1.45% +LC/MS impurities, 8.41% Inorganic
Fluoride, 0.59%+NMR impurities, 1.93%+organic acid impurities, 0.38%+POAA,
033%)
Total impurity from all tests = 13.09%
Purity = 100%- 13.09% = 86.9%
potassium is expected in this salt form and is therefore not considered an impurity.
PoubrsietryvebdyfDorStChsissgaemnpelre.ally not applicable to materials oflow purity. No endotherm was. "Sulfur in the sample appears to be converted to SO and hence detected using the inorganic anion method conditions. The anion resultagreeswell ith the sulfur determination in the elemental analysis, lending confidence to this interpretation. Based on the results, the SO is not consideredan impurity.
`TFA
HFBA NFPA PPA
`Trifluoroacetic acid
Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid
Theoretical value calculations basedonthe empirical formula, CsF17SO3K' (MW=538)
"This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160).
connz3018A
Page 203
CeIntNreTAEnaRlyItiMcaCl LEaRboTraItoFriIeCs CAOTAEROefFereAncNeA#:LY02S3-I0S18A
|
LC/MS Purity Profile
a] Nfcourtroevm:etsh,TerChees4peCca4tniavdenlCdy6.Cs6TtavhnaedlauCreSds cvwuaerlrvueeecsaw.lacsLuilckaaetlwecidusuleas,titenhdgetuCshi7engCvta4hleualenadwvae0rs6agcseatlrecenusdlpaatorenddsceuaslfiiabncrgtaottrihsoen average response factors from the C6 and C8 standard curves.
Prepared By: David 5. Bell
Datpere
Scientist, Centre Analytical Laboratories
Reviewed By: LJaobhonrFaltaohreyrtMyanager, Centre Analytical LaboratoriesDate
CoAn23018A
Page3of3
iElCOdTlOiXfICeOLIOGnYt&eArNAnLaITtICiAoL TnEaSTlIN,G SLERtVIdCE.S
Sepiember 27,2000
M3sM. CSoursparAsn.ioBneach E9Sn3Mv5iBBruouinslmhdeinAntvgae2ln3Lea5b0or9atory PS.t0.PaBulo,xM3i3m3e3s1ota 55133
Dear Susan:
Enclosed please find AddendumsforWildlife Intemational, Ltd. Project Numbers 4544-101,
454A-102, 454A-103A,54A-104, 454A-105, 4544-106, 454A-107, 454A-108and 454A-109
Ifyouhaveanyquestions,pleaselet me know.
=
A
KEeneopnin
Kurt R. Drottar `Senior Biologist
659% Commerce Drive- Eston, Miryand 21601 ToL 10.822 8400 Fx 410-822.0632- Emad ecotonavwidiemamationaicom
EuropeanOffice: Overscheenseweg 1A- 1412 AANaarden-The Netheriands-Te 31(0)356949261 -Fax31(0)356948229- E-mail secpoid@woridoninent