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ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE .IdentityP:erfluorooctanesulfonylamido(ethyl)acemtaaytea;lsobe referredtoas PFOSAA, FC-1 28 or as the major component of FC-127, FC-1 29, FC-109, or FC-1 09-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,sium salt,CAS # 2991 51-7) Remarks: The testsample isFC-127 (F-6390,Lot 1). Current informationindicatesitisa mixtureof40-44% testsubstance,32% water, 14% isopropanol,4% ethanol,and 6-10% C4-C7 fluoroalkyclarboxylate compounds. The followingsummaty isabbreviated because the testsample isa mixture of the testsubstance in a waterlorgank solvent solution. Data may not accuratelyrelatetoxicityof the testsample with thatof the testsubstance. METHOD: Methods: EPA protocol. Test type:Staticacute 'GLP: No Year Completed: 1981 Species: Pimephales promelas Analyticalmonitoring:Temperature,pH, DO, and conductivity RESULTS Nominal concentrations:Bk control6,50,870,1150,1550, 2100 mg/L Element value: 96-hourLCso = 863 (796-950)mg/L Element value based on nominal concentrations. Remarks: There was a slighsteparationintestconcentrationsafter24 hours (surfacefilmformation).Testinwgas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The value reportedappliestothatmixtureand not thetestsubstance. No attemptwas made todeterminethe impact ofthe presence ofthe organic solventsor what portionofthetoxicitcyan be contributedtothe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-12796-houLrC50 forPimephalepsromelaswas determinetdobe 863 mgAL witha 95% confidenceintervaolf796-950 mg/L by moving average angle method. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,utlacksanalyticaclonfirmatioonftestsubstance concentrations. There isa lackofcharacte@zationofthe testsample. REFERENCES -Thisstudywas conducted at3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 7009. OTHER Last changed: 5/18/00 ENVIRONMENTAL LABORATORY AOUATIC MXICITY DATA SHEET EXPLORATOR* (IKANGE-FINDING TESTY Llt Type Testf&e,. torial Testod.4@- Lot Test organisotol6m MiLiku)".=* Avg. Wt. n.ziri5 Avg. SiZO5,Q@CM Test Solution Volaft 3 Liters/All-Olms Tank ft . Orgmism R*cl&.A@@ per &se TO& DisMienst.WIDE-MOUTH Batch No-s@l 5 I GALLON GLASS JAI Tim Started lu Date Started Date complot*d z@4@z Dilution Nate? CG*M-ftltorod.voll water (To". 199-OC.A 0.0. ppm, PH8.7, investipuw (Pull Siong Unit. Tat conc. Total A@t AMM Z4 Total No.Do" LC (93% Confi&mcs Limits) so W$ 0.0 sm 45 raurs Total 0.0. No.Dood YU -72Total No.Dood o9t6ai11 P.O. I no ILo.Deaaddt i:k Cmtrol &/P,II (r)) Ian 0 ilic kilmtm co@ts: kulyiNIAL-1 tank 'Control ki H@z@v, w,lovr,* 1 The ~od As LF ;:two. filtr==Act t ir 4e 0 11 -A I't .1lk.11 .(1) A c@: II&M I 4h TV& -Teft ZNVIRUNMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET DEFINITIVE (PULL-SCALE TEST) LR Material Tested' Test Organim M ILikiagpmnre4<LWTZ ElAAESvapate Rae d eze,,!@El r Batch Avg.Wt.Q,2, ,SL grams Avg. S12:6-U@ CM No. Organism per dose Lceding Factor -016 Fish Disease Treatum t:- -- g/.l Test Solution Volume d6k.4tws/A,U-Glass rim started Started T'Mr* TO* All.- Dimmims: I Date 3Sx2o cm and 25 an deep Dilutix3n Water Cwcbork-flltocvd wen c@e- oe, (ftllSignature) Ta* initial ltbtftal 24 Hm= No. Oxic. hwmt Total go. mj/l AMW Dftd matex (Tmp. lr'5 (95% OAS Aa 48 no= Do0. "l ND. I Dud 14r Con Cop, D. 09 pH =07, le19krts C \-S r f- 72 Hm= (7 7 6 96 Hours D.O. Total lb. PH D.O. Total No. PHID.O. ppn Dead ipm Dead PPM Irr\n% ie) imt"u Data lb. Syopbom cw iftactioa 24-%V. i A- i,.k@o ZNVII<UNMEN'I'AL LA130RATORY AQUATIC TOXICITY DATA SHEET DEFINITIVE (PULL-SCALE TEST) LR_ rype T-@e@@@@@@terial TL-std4l:C- L @@lt restOrganismlngnfA%D-MIHNQLLESiSggOeu,-rOcpteel!Ri@eac'@ Ba tch kvg.Wt:@), % I grams Avg. Siz%.WhaCM Na@.,Organisms per dose 26 ,'ishDisease Treatmmt wt Solution Vo iume I!WIOO(WE IA tk-rs/AU--Glass Tank Tar* DimwAims: landirq ftctor lolls I 35x2()cm ard 25 cm deep 'ime Started Do Started Y&IlbL Da te Ompleted dbo-im $Utmr Carbm-f Utand we wow (TMP. irantigater tcr_n (951 Z- C.p D.0._6, !I2 _pM ts) pH_E,,IS- Ptd1 signah") TW* Initial 7btal ?Mbo. Oorc. kwunt M/1 Ad&d 24 HM= TotAl no. Dvid D.O. wlo 4kKiAL 48 Ham tal No. DoW 72 Hm= 96 Hmws D*O. TOW No. pH D.O. 7btal No. pH D.O. Dad AR q A :zlee- s 6 Date CM =M/ m #Ubivtm =I I f -uver r lb. Symptcus cc rawtjdw gitz1 244k. tLaEdc tUC-3 OA--k LUC I watia wamw 4$4k. 724k. Ai in FR7R@SL 96-W.