Document 7jm346JQ9OKjoL0yZYDrzN2B
Ea `ff
-
~
{
AR2_83460!
5
000055
|
AR226 _ S40!
fm
FON dePserifgluoroctanoic acid in plasma [----
1| |
"TToaublteeoffpcorea ntientncs edessf t
I||
D Fri s 2,2 chemicals
1
3 Sampling and
FSoinse
sample
oy preparation
S7fCrooummpimetetierontmnthieemmitoofeacipi
.
--9d
Ti saidnetof
.
SE eens cao ho mthd
Sounsttnmmnten
PCCrerafalotuonceamctcraoenamceeicdnaisexv aotrsnacottfeedTpwehieethni pHo1r0caseiornpaei hn in eort-beutybCc meoto hyls ether anhd ee
.
2D, chin, soins
27F5E16T05S00mwsihaSltctldsrjtectoiyon esyastemeotpcr
= Viaeting ack with deRgoor gis be
y
000056
i
|
|
i
D
ceonftsraivfuagNeT()3000 -4000RPM)voc centrifuge e.. Speedoc
.
1vA0ard,io4as1bal0en0apdijgueostsasblsey1ri0n0g4edsaengd.1ofmHpaimpietltaers)(23. of EPPENDORF)
2101mlmlvoisls(3va.losfwiHtEhWcLoEpTT-PACKARD)
201m hsevialswithgroundgossstoppers
22 chemicals
acteetrotnBeayimethylesher (T5AME)
pbeanpzyolobsroemriudme Aldrich 817905
(neFaFrOpSoe)rffuAclrdroiccthom(aCioctNar,d171468)
'
Perfluornonansc acid (FNS) of Aldrich (Cate. 39459)
23 soins
10163209M).caanrdb2o1n4a5:t3egbuNfaf2eCr0s3pDHe1c0abeyionin(gM:er4.c2k91N0a6H39C1O)3w(iHthertcer od 200
"2s0c%he(077M)Tetraburylammoniombydroside(TBA) nwaterof
.
M~e0.r5ckMkTeatrtabu8t1y8k7m5m9e)riunbydeoxide (184)i
i
eterbegiing: 65 ml 20% TOA + 35miwatermix
2,3 comparison standards
.
23,1 intel solutions
EPnedlcoonrceontcrtataioenasicicmdg(F/OlR). Weigh 25 5:a FFanOwAth acetone odd 25m exactly.
:
cPoenrcfelnutroartainonnnmoi/c alcid (PFNAY. Weigh ndwithacetoneadd 25m exactly Final
2.3.2 Perforoctncicacddition
S0o1u0tmonl(m1cmsgu/rmiln).g Wlistkhscaentoanceetfoinllef,oatdhde5m0a0rk4.PFFOlAcointcielntration: 50 g/n.
2.33 Perfhoracteancidc,dition 2
000057
IN ||
1
~
F5Ino0e01tammmmceaPtirraoinhonnef6l5asgkrsokak.i,h c1e00rcnPFOA dation 2 23.4 Perfmemmmeic ocd daton | Grenson)
5a010k.ntFomotecnioncg olkoentkhei1n0t,4 ENA itil sao Lng. With acta ithe
235 calibration standards
OPFOAA s coknreicmabdrenebeyendisStt0io0n0imweoliigntplahsma cobrtin standard, ic canton
[--
,
VawG5onvma/oof)thepPtFOmA deaticon |t50s/nov)elcoonomftohe FOardaition
dfs =. po
50O/4i0055505000-215041-00551205-05051405~505~50005-0100 U 05.100
etFprnegeush.teiotrnaasngrcdo1an00dc1ri3 m0t0oc0ee1s5a7rt,y wFoimrmeideincierniemaegnstousffwf8ihoceetthsertcocaotiopnstandrds,
A
31 anpalndsnongi pegratin
`Sampling:
boSeorcdeelivrnttfheordeebrecrboyksernrof ETDhTeAs-oSdtweidtMhoenrahe.sht ela e ricpohcnn Excans CLLiFThAeobvnermd, e2g)oamsodres0p.t2ed5 tndeCcnotarepbodrrHiorStldetSs el pcein siCoemsicnoovsheurtahsTdAnMa aEtoardpn5r eVgrEv)rTdO,|e OO TLYenToeoolr4ro0mnegeM esr eoonn S017 mcsTOMEsnr! io Atom cope rn
000058
.
||
`hwoiehtrSheaakprpirSroonx.n13n050pm1Caun0mn0dreocrcaeri rF5oolCdowsrtkooodsrrdyveanseastrnetst2rie0c8htsob (is uncrie tical)
:
(r7 e msahna eosnhbet ccEoorpdhdne eeoodfnsn dheatremeiscscthe sete)
+ Cottons of mark errr
||
FJaDietltbeyrcatyo5r:caKfeOrO:IAKAEX1e2;s 00m3G1pmmhcr0k3)5ege wsnscLaoaixnOT sBtl:ode8s e85n07s
!
Ector: gn bcs ah 550 copeohsne Sts,
.
|
`LtIasNkEo:fs2a8m0polCeedleeaccttriovnactoelsl:isi2oundiSnpzlaittileosrsst7im0e:eV1mintransfer
EfMSacondtnons msc fo FOR: /2 50a 05 Qui msc
NCCOBEUerSMccSochoonoditromioronpsm:reFogO4R6n:130Dt1w5oaiGn gSnnhpHosvtsntALmeiaftor36nPns:
~
tacrept
lis inecret in cach se he.
i
CpnrealidGyehgifdtledencairrtcnyefcd wonlbteh4rhemessed vis be
scatman
FEoornphedaprrlsoetrorsnactoaescnaorbtrtiop ocievpeomarin onsaeiseedentd
analyzed according to sectio4n bygaschromatography,
OSfnenhtaebdrhaemcea -- ve. heasctedcncnrationn the
000059
|
Stiortionan utysere
|@
Foeqetyteeseancawilpt eace o e the TRgA h 41 ttheedberrgrasstteertseuloton
i
J----
Simeon
Free--r--g--sigpt h aie
i
Sf pingnrssr icon Ar theeese Frroedso
Sst
,
To recessofth peste no omnintyreprgspaepnrt
spbinggnsRurcoHenmry 10o10o57 6931072510981 18207051099 E0038 105 ~: Sm ctr comparisonp , ean ofr FON ep
oe ese Ts Tea ne Person ET-6CMSNCI MSMS19/115/119.61082546633354 spermine
pR UrnodwerietheSemianmcirpteemtrEiornaiasdofoe i mm ek Me atinlgses 7o%nte Teramoto te re08
94 cassie 170K festemprcvies cf prs05 eeshied
000060
A
1c51. Tbhcaoserperso ftltyeesiCdroynt Tedega
IK)
comitedm aFO 0fos.
|
Adirption Gs ra licneil nedidn sre.
--
Toe hepclroe6spn aai5stoeneFdofbnhobeesueanotnitthenrposrfcoEocOpee
hroemhteentodhST ennrete s eots ibe(moA po
;
eF eXenret nvte BemarilyS rToO,RE3eerdrgypeodre 1
eriecosempaapioif oovfes30he2econlddseyomebesr tepseed
Eie EnE moa iXnSfo3e0S1rm0A5GSersnneOOamedemce0dcof, s
~~ D
he <F01190r5 ymlt.esinceoinmr theemoremcoencnengtraterde seostaensheebernet
i
es on oihtoo)
prteeectciionc1non6etkMe oTeoerwyiehanHoieynnstohr,eNEA6S5EGEMNeCSEronreEenTGeeEeMS
oretrremattctymneehneteshaceoigehrbSCeeetsetGEamEthBsNtbGopeneponroatnaLe
cpyeaparaTors31caaovm ietEEn OSa syehe gt)
T ie oS nt vegede----
000061
. @
e T rte m rpse e eec e e edretheepreeeei tec,
ree HPLC-MS (2).
T dA meeer r dm et Setene
SB9.r8lmtrieemte smtsovtens Gpoonn tttttgcmee
~
000062