Document 7a4OJ0zKLeqvEG1rggerR30a
BIOACCUMULATION (ANALYTICAL ONLY)
TEST SUBSTAN
Identity: NN--eEtthyFlOpSeErfAilucoorhooolctoarneFMs-u3lf4o2n2a.mi(d1o-eOtchtanaonle;sumlafoynaamlisodeb,eNr-eeftehryrle-d to as C1,A1S,2#.21,639,13-,49,94.,25),5,6,6,7.7.8,8,8-heptadecafluoro-N-(2-hydroxyethyl)-,
Remarks: Material is an off-white, waxy solidof uncharacterized purity. METHOD:
Method: bDieovaicsceudmbulya3tMe fCluoomropchaenmtiyocadlestferrommin3eMif'bsluDeegcialtlurs,unAfLishplant effluent (See Remarks)
GLP (VIN): No Year completed: 1978
Remarks: The following method was devised and used:
1. 2.
Twolots of 30 fish each One lot was exposed to
were used. Decatur plant effluent from
Oct.
5
to
Oct.
25,
The
other 25.
lot was
exposed
to TennesseeRiverwater
only from
Oct.
3 to
Oct.
3. TawnedltvheefrieshmafirnoimngtheeigefhftleueenntfeisxhpwoesreedslaoctrwiefirceedcoilnlDeecctaedtudre.adTh(edireidveirn test)
wsaactreirfiecxedpoinseDdecfaitsuhr.survived through the exposure period and all were
4.
Effluent-exposed shipped in dry ice
atnodthreiv3erMwaEtnevri-reoxnmpeonsteadlfiLsahbowrearteoralyl
preserved in St. Paul,
and MN
for
analysis.
5. 6.
Fish Two
w2egr.epohrotmioongsenwiezreedweusiignhgeda
Tekmar Model and extracted.
SDT One
tissuemizer. portion using
ethyl
7.
aScaemtpalteesanwderteolauneanleyzoendthbey
other. GC, TLC,
and
GC/MS.
RESULTS
Analytical Observations:
Twehiegehfefdue3n3t.0-egxrpaomsse.d fish weighed 25.7 grams. The river water-exposed fish
006276
GC/MS effluent
acnoanltyasiinseodfe7tphyplm
aocfetNa-tEetaFnOdStEoalluceonheole.xtracted
fish
exposed
to
plant
No fluorochemicals were detected in the river `water-exposed fish.
fRleumoarorckhsem:icalsS.tudWyawtears ucsoendduicntmeadnubfeaccatuusrein3gMi'sslaDteecrattrueratpeldanatnmdatnhuefatcretautreeds
effluent discharges eventually into the Tennessee River.
CONCLUSIONS
No reliable conclusions can be derived from this study. SMiunbnmeistottear,: 5_531M33Company, Environmental Laboratory, P.O. Box 33331, St. Paul,
DATA QUALITY.
fRaecliilaitbyilriattyh:erKtihiamnisacth3rMa'nskiEnngvi3.roSnmteundtyawlaLsabcoornadtuorcyt.edEfatfltuheentmaconnucfeancttruartiinogns of
subject fluorochemicals were not characterized and the specific protocol for
tehxepoTseunrneeosfsethee known to cause
RfdiievsahetrwhwadsuranionotgtrfeNofuPefnlDdu.ErenSTthtueessrteeidnwgi,ansotrhaellsosowtunDdoOy,i(nehf.igog.rh,maNTtDiHSo)nN.oninaenfafllyuseinst
oisf
Aprdidoirtitoonaslalcryi,fiitcei.s noPtreksneonwcne iofftthhereetewsatssuabnsyto ancpepcooulr d fhtoarvudeenpbuei reanttiaoynnoorfmtahle fish
functionofthe fish concentrations homogenizing with the effluent concentrations.
ecNhfoelmueiexcnpatlla-newaxtapiososnaecditsugaarltoltuyepam.pctcNeuodmudailsfaftteoeredwnhtiainattthiwoeanfswisathshoergcisaviuemnspeltyoofswohtrehbeteh1de2rodntehtaehdseufsbiujshercfitancteheof
the fish body. Sampling locations were not described.
REFERENCES
3M Technical Report "Analysis for Fluuorochemicals in Bluegill Fish" G. A. VNruamsbpierr/ 1A4r,thMuaryMe1n,d1e8l7,9Project 9970612600, Fate of Fluorochemicals,Report 3M requested expert overview, `Bioaccumulation Studies", Dr. James Gillett,
Cornell University, March 8, 1993
OTHER
Last changed: 5/17/00
006277
5M REPORT BIOACCUMULATION STUDIES
Maren, 1553
(Som 159 i Parti n er eare pcrAesbeonoteudnint.iveproycrmtaorogmfifneearlsniszteodmmadnnert. Itexipvleasnatthieonimopfrsesslionythatpderso(cheysdreomsypeatethioysn ouf Nrrndpdaos
006278
L%r
3Femmina
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.208
Imp=oreprort tianednonoth siodfpeows,ec wocop0TeCCs.)
=orm
Environmental Laboratory (EE & PC. Fate of Fluorochemicals
Req. No. 48765
D. L. Bacon Analysis for Fluorochemicals in Bluegill Fish
0535 .
014
crG. A. Vraspir/Arthur Mendel
48838-18
seEoonnnst,d aOcoCnomtmaemnit)GRcSTivEm:onton | "SSE >
Date Soe
Status Report
ori 76416/43939
~
7
Senkiogres -7 :
REPORA TABSTTRALCT,. (R200h750wreTtSres alien ened TnCorio So
iR.A. Prokop-236VARl.INPLo.WtBeholahtpoernra-g23a11d--a22-286-3A
tegen is J
.
006279 ;
7 FC (Req. 48765)/GAV/AM
-2-
5/1/79
OBJECTIVE
Tifhlseuho,rpoucrahpfeotmseiercaoelfxsp.othsiusreitnoveosutrigDaetciaotnurwasplatnotdeetfeflrumeinnte, ifbibolaucecguimlullated
SUMMARY
ET(xLPtCE)raiccntodsnitcaafitrneoedmdbtlh1ue0egppiprlmelse(nufcgi/esgh)ofexofpFCo-sF9Me5d,39t2wo3hiDleaecnadtFuC7-r1p4pp3lmanoatfndeFFfMfCl-3u412e22n8,t were not detected.
EeNtoxhtarfnaloculto,srocfehtrheoymmlicbaleluthseegriw,lerleetfdhiesothxeyceettxehpdyo.lsedacettoatrei,vertowlauetneer, (RaWn)d cXyolnetnaei.ned
eAxpceorpiymeinstsa.ttached of CRL report 7124 which detailed GC/MS
INTRODUCTION
Tbyhefuipsthakweereandrepcolretaerdanc(e1).of sThpeecicfuirrcenrtadireoploarbteleddetafillusorotchehemicals Daencaaltyusresplfaonrtfelfufolruoecnhtemiacnadlsininfisfhisehxpproesveidouosnllyy etoxpToesnendestsoeeour Rtoigvrearphwya,terand(2)g.as Tchhirno-mlaatyoegrrapchhyr/ommaastsogrsappehcytro(sTcLoC)p,y (gGaCs/McSh)rowmear-e used.
DISCUSSION
5
:
OmeuvarennutfDuaeacclatltuyurrinigpnltaoinstthlemaatneTurefnantecrstesuaerteeesdRifavnleduro.rtohechBeltmurieecgaaitllesld.effisfWhla,uteenrcthoussdeeindschifanorrges 5 tfhiesshewebrieoasesxapyoseedxpeornilmyenttos,thweerTeenneexspsoeseedRitvoePr.Ew.aterAno(2t)h,erThleot of ithsrheew-erroeutethepnrocaendaulryezedasfoorutlfilnueodrocinhemFilcoawlsDibaygraanm 1invanodlvdeedtailed ainndthseomeexpoefritmheenthaolmogseenctaitoen.wasBreixetfrlayc,tedfiwsihthwetroeluheonmeog(eRnoiuzteed,1), w(hRiolueteso2)m.e ofA ptohrethioonmogoefnatthee wlaastteerxtreaxcttreacdtwwiatsh ectohnyclentarcaetteadte Rseocuttieon3.) for GC/MS and TLC. Results are given in the summary
006280
v
-3-
K
:
FLOW DIAGRAM 1
ANALYSIS OF BLUEGILL FISH
30 Fish
.
((21)) W"eTiigshsuenize"
3
,
5
<
(1) Weigh 2 g. (2) Extract
(ETOAC) (3) Centrifuge (4) Isolate ETOAC
Layer
Route 3
ac
FFoMr 3923 TM 33492225
Concentrate
ml
Ge/us
FToer FFCC--19258 FC-143
(1) Weigh 2 g.
(2) Acidity
(3) Extract (Toluene)
(4) Centrifuge
(5) Isolate
k
: Toluene Layer
(6) Methylate
Ll
Gc/us
*
oc
5
Toles
(Ester)
006281
FC (Req. 48765)/GAV/AM -1-
5/1/79
EXPERIMENTAL
:
Tbtwyiovetlhlyeo,tsEnavnoifdrostnhhmiieprnptteyadlfiinsLhabdoreryaachti,ocre,yw.rwaeprTpeheedrfeiincsehiavlweuedmrieonnukmNepoftvoeilmf,breorsreen3s.puenc1c-9i78:,
aOcnta.lyz25e)d eighteen
wf(eiNiosvgh.hewde3,re251.s07a78c)rg..ifiTcTwehedelveienffDlfeuicesahnttu-wree.rxeposcTheoedllelrocittveedr(-Oedctxe.paods5eadntdolot
a(tOctD.eca3tutro. Oct. 25) weighed 33.0 grams and were all sacrificed
:
wTaos siamnpalliytzye,dthien were used:
eexxpaecrtilmyentthaelsaomnee
mlaontneirs. disTchuessfeodl,iowTihneg
octohdeers
River River
Water Water
Holding Holding
Tank Tank
Exposed Exposed
Fish Fish
-
teexottlhruyaelcnatecete(axRtt.era.c-tEtAc)
:
PPllaanntt EEfffflluueenntt 5S--11 EExxppoosseedd FFiisshh -- teotlhuyelnaec(eRte.xatt.re-aTcoetlx.t)r(aBc.t8.-(TPo.lE..)-EtAc)
TTtuhwbeoes2f.igs.h"Twpweoorretmil'ohsnosmoofwgeernpeiezsetwdieciigudhseeidnggrianadteToekesmteahpryalraaMctoeedtealptoelSyDweTetrhteyilsesaundeedmeidczeetnrot.rifuge
otVnooerttehaxendoGte2hneimrel. mcoinxTcea.rc.hHCslTahmepplleussawmap2slemslmixowfeerdeAaRpcpgerrnoatxdreiimfauttgoeellduyenfeo1rmwie1nr5.emioannd.deadat :
1oefx0ft,r0a0ac0ntdrwppalms'asc.eedxapTihinenegdelatushssyilnavcgiaelttsah,teerfeaosnlpdleocwttioinlvgueelnGyeC. ceoxTnthdreiatcitoostnhswy:elrseceItndsarttarew.n -
HT1P0e%mp57C1a-3rb20ow0wigtxhC.2Ni,0> Defoe.nleT6ce0tm/rp8o0n-mce3a0sp0ht,uCrheCr.o,dmeoOtsveoecrntohrT.We-mApWC.-olu15mI0sng.-C P6o'irxatot1h/-e8r%ma5l.;5.""y3
Flow - 40 cc/min. Argon/lethane (95/5).
uAnfdteerr NG,C paunragleysifsoroftwoEtAdcimeexntsriaocnta,l TaLCporatnialoynsewsa.s
TLC confiitions were used. The concentrated Eth
cTohnecenftorlaltoewding
extracts were
-
MsineproEcttktAecdsilaanildcoandgrsgiieeldde.plsattTaehnsed.nardtshTeheoypflawFteCe-rs9e5,wteirrFesCt-1t4ud3re,nveedlaon5pd0e0dFO-ia1nnd281d0e%ovneElEE.oOpHed
wiinth50%0.0B1t%OHNeiwn EPtuhcch.sinThien wdartieerd p(l3a)tes were visualized by spraying
e1TxhgegitneoelldeucetnuresoinnegxctartaphcteturfewoalsdleotmweeicnttghoyrlG.aCtecdCoonldwuiimttnhiond-si:1a2z'oxmIenst1th/r8a."ne5H.Pa5n,dS712t30he%wnith
24D00C0-3c0o0/C.miann.DdetA1.r0g%otnBe/empMnetto-nhea30n0e34 (Co9.r5,/58)O0.v/e9n0Thm-ees11he0thgyalsCakcrIaostmoattPhAee.rmaanldi,njm.eFtlPhooywrlta~te-d
Gt2C0o/TlMuSGeCn/eaMnSeaxlytcsroinasdcitts(iRoewnqe.sr.eNos.ubmCi-5t0t6e8d7)t.o CSeenetraalttaRcehseedarcChR ARenpaolryttiNcoa.l f7o1r24
-
006282
. TFC (Req. 4876S)/GAV/AM
-5-
5/1/79
:
PREW/E&tAPcE-Teoxlt.racsthoswheodwendo daipfpfreorxeinmcaet.elyNo10FCu-g1/4g3oforFFMC-3412228.was
:
dReetweacstedthabty TofLCroeffertehencePEFEC-t8c5,. but a spot was noticed whose
REFERENCES
(1) a) BM,lueT.gilEllnaSbuanrfaiwsyh, an"dBiIoncoCnhcaennnterlatiCoantfiosfh,F"M E3E422& PiCn
Report, 5/17/77.
Lo
b) AFi.N3.422W,el"teErE, &"PACquaRetpiocrtF,ate10/o1f4/A77F.luorochemical,
(2) DOcetc.atu1r8, Ef1f0l78uenatndFFiesbh.To5,xic10i7t9y; TeM.stT.ProEtloncaoblasr,awyd.ated
(3) 2J3., Sl1i21wio(k197a8n)d. A. Macioszezyk, Microchemical Journal,
84 Vegi so
|
GAV/AM/cen
Attachment
:
.
-
006283
----
CENTRAL ANALYTICAL LABORATORY
Report No... 224
Date. March28, 1979
Subject: Decatur Fish Bloassy.
Requestor %. Vraspir,A: Mendel
ReNq o,u C50e 687 st
Dept. Name EESFC____
+ Dated November 16, 1978
po No. 215016130404
Report: INTRODUCT 10M
:
TDDeeenccnaaittauesrresPFliaRrniatv.errecwFehiiisvchehdweffrlreoownsextpJhouessetrdeoquuttoessittdowerosdiffrfoemreantfiwsahtebrloassyssyrastearkenohat the
:
dTulaaaznoannstthehaacnGaeCi/ManSnedsayrnsiatvelemyr.z.ed.T"Eoclhuyelneaceetxattreactesxttrohafectptslhea`notffisathnhdeweftrlheeehmpwelecarhneytlseberraejl)vveLenintosn
DisuCssIon
71wA22aer.vewee5rr2geeanaplandenisatlayPwzeEersdein1lg2abce(olsnetddhyi]tRi.Woa.ncset/a1ftoeranedcxotlPru.aEmc.ntsA#)1.be(lmoSewatmhapynlldeastseaAd.mVpt.loelsu1eRnoeeun.dexts4r3eaccatss)) and Le
using conditions for column 8 blew
nt Pc.
SeIIonnljoseutcmrtnuomreA1::n0t":C11,6l25V2a0r5ti.h5ae.nrn22a07l%40fDoCgr-as20110chm+rionm1ua0tt3eosgBranattphohernie 13140-o1n70AnCakerromT0sPeA/min.
.
|>
OCvoelnu:mn 5B:0 Injector:
-26!220255C.C5. atCW1200HC/m1i5n%. on
Chromosorb
W
5
CDaertreicetorr:Gas:VariHaenliufmlaante 2I5oncicz/amtiino.n at 290C -
.
fSIlonoudsret:creu:mean1tC:hesnalmdcpualPleosntIwoe2nr1ie-z4at9riu1no8nmianssElescptercotnromIsotneizration, oe; *135
sample
P..
#2
HHaeslsiumRanSgeep:arat1o8r:-1426,1Si7InngmlCjeheem.SitcaaslegoIotnization with Ny gas 3
Multiplier: Bendix Channeltron
006284
-
7 Renort vo. pizk
x
rio, ar
RESULTS
Samples R.W. /1 and P. E. #1 showsd by GC/MS only the solvents used in extracting and methylating: ethanol, ethyl ether, ethoxy ethyl acetate toluene and xylenes.
It was necessary to alr concentrate the ethyl acetate extracts in order to
obtain sufficient data to Interpret the mass spectra.
appear to correspond to refarsnces of 2-methyl-3-octanons, acetic acid, & The Tennessee River exposed fish ethyl acetate extract contained numerous
G.C. peaks none of which appeared to contain fluorine. The`components: phthalate, and high molecular alkanes from aboutCis to at leastCon:
acid, fluorochenicals whose highest mass fragments sean vere AB) We and The plant effluent exposed fish ethyl acetate extract contained compounds
F:
which appear to correspond to references of 2-methyl-3-octanons,
tic
582 m/e, nitrogen containing fluorocehmicals (whose highest mass fragments seen were 448 m/e and 584 m/e), FH 3923 (FOSE emide) and FM 3A22 (POSE alcohol).
SUMMARY
:
River water contained such conpounds es ethanol, sthyl ether, ethoxy ethyl This fish bioassy showsd that extracts from the fish exposed to the Tennessee
acetate, toluene and xylene. The extracts from the fish exposed to the Decatur plant effluent contained FOSE amide, alcohol, other related fluorochemicals, 2-methyl-3-octanons, and acetic acid.
i Jeli
9. N. Schrospfar
v
Ich
?
.
006285