Document 7R4vzOE0kKrJR2zVMBrLMR0QB
AR726-0508
CHRONIC TOXICITY TO FRESHWATER INVERTABRATES (Daphnia magna) TEST SUBSTANCE
Identity:
Perfluorooctanoic acid, ammonium salt; may also be referred to
as PFOA ammonium salt, Ammonium perfluorooctanoate, FC-
116, FC-126, FC-169, or FC-143,
pentadecafluoro-, ammonium salt,
(Octanoic acid,
CAS # 3825-26-1)
Remarks: The 3M production lot number is 264. The test sample is FC-
143. It's purity was not sufficiently characterized, though current
information indicates it is a mixture of 96.5 - 100% test substance and 0 --
3.5% Ce, C7,
lot number is
and
264.
Cs
perfluoro
analogue
`compounds.
The 3M production
METHOD:
-
Method: USEPA-1982, OECD 1981. Type: Semi-Static Life-Cycle Toxicity GLP: No
Year completed: 1984
Species: Daphnia magna
Supplier: In-house cultures, 3M Environmental Lab, St Paul, MN
Analytical
Exposure
monitoring: Temperature,
period: 21 days
pH
and
dissolved
oxygen
Statistical methods: Probit analysis (for acute toxicity values) and
Moving Average Angle Method for chronic study. Statistics were
recalculated by AScl Corp., Duluth, MN in 1998. NOECs and LOECs
were generated using Toxstat. Reproduction was normal and
homogenous therefore Dunnett's Test was used. Fisher's Test was used
for survival. ICsos (reproduction) were generated using ICp program.
Survival ECsos were generated using Trimmed'spearman-Karber.
Test organism age: < 24-hour old neonates for test initiation.
Pretreatment: None
Test
conditions:
Dilution water: Aerated carbon-filtered well water
Dilution water chemistry (sampled one monthpriorto initiation)
hardness: alkalinity:
240 mg/L as CaCO; 230 mg/L as CaCO3
PH:
78
Conductivity: 340 pmhos/cm
cop:
<0.4 mg/L
study
Stock and test solution preparation: Information notavailable
03824
ERxepnoeswuarleravtees:sEelvse:ry25tw0o-dmaLygslass beakers containing
approximately 200 mL test solution. The depth was`approximately 5 cm. Beakers were covered with watchglasses Number of replicates: 4
LNouamdbiengr:of1 tdeaspthonrigdapneirs4m0s mpLer replicate: 5 Numberof concentrations: & plus a blank control
Feeding: A suspensionoffish food (irout chow) and yeast bcoanstiasining 5 mg dry solids per one mL of mixture was fed on adaily t<L.riagnPhsthiiotnitogon:peprCeiroioooldd-o.wfhi1t6e-hfoluurorselsicgehtn,t8l-ihghotusr.sIdnatrenkswiittyhraan2g0e-dmi5n0u-t1e00 ft-
Water PchHemainsdtdriysosfolnveewd aonxydgoelnd:soIlnuftorimoantsiodnurniontgavtahielasbtluedy: Test temperature range (0 - 21 days): 22+ 2C
Element basis: Survival and reproduction.
BREESsULYTSS
ENloemmiennatl vcaolnucee:nt1r4a-tdiaoynsN:OBElCan(ksucrovnitvraoll), =5,680, 1m3g,i2L2, 36, and 60 mg/L. 2114--ddaayy NNOOEECC ((sruerpvriovdaulc)t=io2n2) =mg8iLmg/L 2114--ddaayy NICOsoE(Cre(prreopdruocdtuicotni)on=)4=02(228m-g4/8L). mg/L 21-day ICso (reproduction) = 43 (35 -- 46) mg/L
All element values based on mean measured concentrations
Biological observations
aSturtveistvatle:rmAillnastuirovni.viSnugrvfiirvstalgeinnetrhaeti3o6nadnadph6n0idis Lappteraeartemdenntosrmwaals
statistically significantly different from the negative control group.
Reproduction: Daphnids in the control started producing neonates
on Day 7. Reproduction was statistically significantly different from
the control (Dunnett's) at the 13, 22, 36, and 60 mg/L
concentrations after 14-days,
solutions at 21-days.
and
in
the
36
and
60
mg/L
test
CO3825
Summary of Percent Mortal
[Nominc al |oo or n |omn ar| NCeognattriovle
Reproductionafter 21-da
Nominal [Numberof] Mean Live
Concentration, | Surviving| Young / Adult
[Lsne eop[a[wv02eo0|[|cuoaumsemeerss|||| g
Daphnids|Daphnid(+ SD)
2||e een | L| oo wew|
SOe CNONCCULIUBSIIOONNSerie `There were no adverse effects on survival or reproduction Daphnia magna
exposed to the test substance at concentration <22 mg/L for 21 days.
BAuotxho3r3a3n3d1/,oSrt.suPbamuilt,teMri:nnes3oMtaC,o5m5p1a3n3y, Environmental Laboratory, P.O. DATA QUALITY
Reliability: Kiimisch ranking 3. Study was apparently well conducted
according to the methodology available at the timeoftest initiation.
03826
sItnfuodrymcaotiuolnd wnoatsbneotloacvaatielda,blaendonstoemsteskuebystsatnucdey epulreimtye.ntTshceourladwndoattabefor this evaluated at this time.
REREFFEERRENCCEESS ~~~00
This study Paul, MN.
wRaecsalccounldautcitonedofatthteheef3feMctCaonmdpnaon-yeffEencvtircoonnmceennttraaltLiaobnosrwataosry,
St
performed at AScl Corporation, Duluth, MN
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
e0 OmTHeE0 rR 00000000000 Last changed: 5/25/00
03827
Sh
,
ENVIRONMENTAL LABORATORY
ALES
DATA SUMMARY REPORT ONTHE" TESTS FOR ACUTE & CHRONIC TOXICITY OF FLUOROCHEMICALS
TO DAPHNIA MAGNA (WATER FLEA)
(9972512600)
TEST sussTANCE
Description: FC- 143 Lov 2H
Additional Identification:
STATIC ACUTE TEST
DBoesgeinR:an8g/e:2/2S8,4 4e0n,d:(358,/140/0,814:0,Du2r5at0i,on4:0N0G,3H0ouwwelsl
Temperature: 22C.
Results: 7 24-Bn.
Bogy
(95%
Conldence
LimAlli(St blos ~41) 2 wi:g _
= 4B-Hr. ECG, (95%C.L.):2bb (231-30"1)uUgl
RENEWAL CHRONIC TEST
Begin: 8/7/84 End: BRB/BY Duration:2| Davs Dose Range: $,8, 15,22, 30, (oO wif
T
.
Temperature: 22+ |9C.
Results:
Young Reproduction
- Approximate NOEC
Impairment 15
== MMA-TCDa(yLiEmgigts)(G95M8-MCAoTlCo) 3|M ((1220--2425))
=
2-Day Begg (958 Cul.) Chronic/Acute Ratios
3g(55-42)
~ App(lMiAcTaCt/iAocnuteFacEtCoggr)s
- Env. Safety Pactor
Adults
Mortality mall
wall
SLO wy
HO(31-52) wall
(MATC/EEC)
93828
* A stedistical significance test Was peformed. using Pro.0s.
.-
-2-
ACCEPTABILITY OF CHRONIC TEST
.
The D. magna control population have met the following
quality criteria:
= Adults Mortality:
AONE (max. 20%)
=
=~
Pirst Brood Release:
Cumulative No. Young
per
Adult
7285S (max.
(14-Day):R(min.
9 days)
20)
~ -
Cumulative Ephippiar
No.
Young
per
Adult
(21-Day)ikmin. 40-60) Aowe(none)
- D.0. saturation:
82%(nin, 60%)
~ pH Level:
B.otozmax, 10.5
. from initial)
COMMENTS
-
Reported and ApprovS ed by: pi
vars 5/05//85
ze
03829
ThOLE I. suMkARY OF ACUTE ToKICITY OF EC 14%, Lot ZI
TO DAPHNIA MAGNA UNDER STATIC EXPOSURE CONDITIONS!)
ausncar TrenTHENT
(mg/1)
suuNmo,kaehxLeToYsune
I
[3]
[AroRrAELITY--
iy
contro
2s
|
40
b1030
Iwo
250
Hoo
I
v3
3, (330conemoncs wont)a
5
40
as
Ho (Bbb- 442)
\
1s
30
ao
100
.
2b (231-301)
(1) Tbataearee aveIratgeaslofoftwoDuernetpyliGcaapthiaoindssfwoarreeaccahcktrpeoartmeenrteawtiatmhe.ten (10) daphnids per
(2) Begg values vere calculated using logistic regression methods (Peabit Analysis).
8&&83
.
ENVIRONNENTAL LABORATORY
:
TABLE II. SURNARY OF CHRONIC TOXICITY oF FP-\S gr 20)
.
TO DAPHNIA MAGNA UNDER RENEWED STATIC EXPOSURE CONDITIONS(M)
cHEncaL TREATHENT (mo)
YOnUeNnGzrRuEePRnOr4D-U(CD4TA)IYON|ExpwosoUArRDrEUaLtTry (v) fl] YOVUTNPGAIRR2EA1PE-RN0OTADrU(C3ET)xIeONo|]surWeORABdAuELETTY (8
conaoL
5
yo
8
20
3
2
22
38
EN
wi
wo
]
ecg,s0 (95 cponoryoence Limits)2 @ m2, (21-42)
335 (20-45)
5
o3r 0
12,
5
'
Io
20
zl
20
21
20
zs
25
1
80
Ho
Hz(34-0)3)
38(z5-42) Ho (31-52)
(1S1 tDaottaal'aorfe tawveenrtaygesdapohfnifdosurverreepliusceadtipoenrs tfroeratemaecnht.treatment with five (5) daphaids per replicate. A
(20 K
ECgg values were calculated using logistic regression methods (Probit Analysis).
(3) ECq, values were calculated using Moving Average Angle. Method.
YoungRermaucnDornm
Cumuasmioe.NosigeR, Test susswice EPSA076 vestoare 5/44-- hs
0%es SEA he Tet Lut
Ror Adult
B22
Ld
INT
dd
Cosmos
1300
18
218 | 1s
X*S | z259|+R=E) L
| om Ber Tet Liat
sz
| edo
qo
| goz
| ezpre
2ri
150.6
168
181.0
100.
pS
12
Sway
~
XS
=
8wall
_
X TS
P=
2000
353
285
BHT
3 Eh |
LO. 50 | 187
3[0P8d
21
25x08 |
0.1408
52,
528
los.
0..0
51
Lay
2x9
Blo'7
113.4
ne
2.0
Hr
155.4
723TH| INS* 29
0.1 |04 .23 0030 .| 0.206
31.4
534
120015%
3L3l..4
aH
HZ
. 310
ais
53%T22.| pygx iz
011908
0, &Siq
113.8
TM
l8s55
138%57
0.381
Ist | soa | we | ae
[Bndmn 21303
2H8o2l.o
5w5i0g6
1n5z4..a2s5
284
Te.8
qu
182.2.
XS | 228500 | do x22 | lpi 20 [OSoEp
P=
0.1080 01080 | ove | sea
2 TEST SUBST
LBRLED
Young RepnaucnonDew
Cumusrnoe Nos
}
2
TesrovegBL 7lk ans
3
Description me Tear 11
Rec Adu
21D.
| Ter Tent Unit [Rr Ad
Cosmos
.
X*xs |
|
|
0 22
wef
XS
2M
2.28 2b
15
22NE 48
HAH
HS. 53.2
3,2
HSE 10
C2
A) 1
511
| LET|
= oo|o oooss g*|a noott a*|
NZ
284+
B50
Bbw) | 2a24w3
H2B..2
5S4b3
184
Bb.
a
Xs
QENZ| zara
523+120|
P=
0.0013*| ocoiz* | 0.014 *
121.8
153.4 M25
1.55
(30% |
0.0047
HM.0S;
lq.o2w,
1241.5
108% 24
0.015) *
_10mg
XESS
LPs
a | OE a " 108 Iss loo
2
Hs
24,33
3]
202_
SLM
20
Iso
31.35
0.4
.
| sz | izes
155230 | SL* 2
0.0001TM* | oocolTM 10,0001| 6 rec
.
MTE/RRR
ENVIRONMENTAL LABORATORY
1-3-84
SUMMARY OF EXPERIMENTAL DESIGN AND CONDITIONS FOR CONDUCTING
RENEWAL CHRONIC EFFECTS TESTS WITH DAPHNIDS
Project: Fate of Fluorochemicals - Phase IT (9972512600)
Exposure: Type - renewal static Renewal frequency - every 2 days Duration - 14-21 days
Test Species: Daphnia magna or Daphnia pulex (use of D. magna is preferred)
~
-
Age
No.
of test animals - 12412
animals/beaker - 5
hrs
instars
(neonates)
~
No. of replicate test Total No. animals per
beakers per concentration concentration - 20
-
4
Tost Vessel: 250 ml glass beaker (Pyrex) covered with a watch glass
Test Solution Volume: 200 ml ~
Loading Ratio: One animal per 40 ml
Dilution Water Supply: Aerated carbon-filtered well water (Bldg. 2) of consistent chemical quality. Water hardness classification: hard (hardness ~ 250 mg/L as Caco,).
Untreated Controls: 100% well water
Toxicant Dose Range: Minimum of five logarithmic concentrations (by weight) Test Conditions:
Temperature ~~ 22+2C (68-75%)
.
Light Quality - Type - ambient laboratory light (cool-white fluorescent) .
- Intensity - ambient lab levels (50-100 fc)
- Photoperiod - 16 hrs light; 20 minutes
transition period
Aeration During Test - None
03834
Food and Feeding Regime: A suspension of fish food (trout chow) and yeast
(Fleischmann's) containing 5 mg dry solids one ml of mixture, on a daily basis.
per
Measurements and Observations:
Effects (noted daily and recorded on renewal days)
= =~
No. No.
young, 1ive dead adults
and
dead;
brood
size,
and
no.
broods
-
Immobility, other signs
presence of of stress
unhatched
eggs
or
ephippia,
and
Water Quality (of dilution water and of replicates of old
test solutions made on renewal days)
- Physical: temperature, D.O., and pH
= Chemical: analytical measurements of toxicant
concentrations in initial stock and in old
test solutions (optional)
Validity
of Test:*
The control population
requirements:
i
of
D.
magna
should
meet
the
following
- Mortality of adults - maximum 20%
~ First brood release - maximum 9 days
= No. young per adult (14 days) - minimum 20
= No. young per adult after 21 days - minimum 60
~ Ephippia - none
= D.0. saturation - minimum 60% throughout the test
- PH - maximum +0.5 units from initial
Treatments of Results:
48-Rr Acute Test
- 24-hr BCg,
- 48-hr EC;) with 95% confidence limits
14-21 pay Chronic Test&--
- Approximate no observed effects concentration "NOECTM
= 14- and/or 21-day EC, with 95% confidence limits
- Maximum acceptable toxicant concentration "MATC
- Chronic/acute ratios
:
~ Dose/response curve based on cumulative mortality of
- safety factor
adults
CO3835
References: - USEPA-1982. Env. Effects Test Guidelines. EPAS60/6-82-002 ~ OECD-1981. Guidelines for Testing of Chemicals - ASTM-(latest drafts, 1983). Proposed Standard Practice for Conducting Renewal Chronic Effects Tests with Daphnids
"Final results are calculated from mean values of cumulative no. young per adult, or cumulative mortality of adults. Definitions:
Daphnia Chronic Toxicity Test - A 14-21 day experimental study of the survival, growth and reproduction of asexually reproducing Daphnia beginning with. 12412 hr old organism.
Significant - "Statistically significantly different" refers to p<0.05
with respect to the control. Approximate NOEC - The highest test concentration that will produce no significant observed effect on reproduction of young or adult survival. Chronic ECgo(s) - The values of toxicant concentrations which impair reproduction of young or induce adult mortality by 50%. MATC - The highest concentration of toxicant that has no adverse effect on survival, growth, and reproduction of a species. GM-MATC is the calculated geometric mean between the highest test concentration having no significant effect and the lowest test concentration having a significant effect. Application Factor (AF) - The quotient of the chronic MATC divided by the acute 48-hr EC, (MATC/Acute ECgy). "AF" is a number used to estimate concentration of toxicant that will not cause significant effect to a population during chronic exposure. Environmental Safety Factor - The ratio of the chronic MATC with the
expected environmental concentration (MATC/EECf. Safety factors >20 at
the predicted exposure level are desirable to have sufficient confidence in the environmental safety assessment.
03836
8-23-84
ENVIRONMENTAL LABORATORY FINAL REPORT
LAB REQUEST NO. B20S5
REQUESTER NAME: RRR DEPT: 0222 PROJECT NO: 06 DATE RECEIVED: 8-16-84 DESC: JUME CARBON-FILTERED
WELL
WATER
CONTRACT LAB:
CONTRACT LAB COST:
0
3M E-L HOURS:
3
EST CMPLT DATE: 8-24-84
DATE COMPLETED: 8-24-84
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
1
7-06-84
RM #4
|
"-
-
=
2
DI
WATER
PH RES=CL
COND
IT00TT--AHLAKRD
NH3-N SOL~-F COD-LOW T0T-COLIF sec
PH RES-CL COND SPC
7.8
<0.02 MG/L
340 UMHOS/CM
222743000
WMeG//LL Me/L
<0.4 MG/L
0.2 NG/L
<0.4 MG/L NEGATIVE
54 PER ML
6.6
\_
0.02 MG/L 1.8 UNHOS/CM 14 PER ML
* = CONTRACT LAB
/
APPROVED AND SUBMITTED BY fo
outer FRYE oo
-
,
93837
& w=Sm = AScl Corporation
4444 (218)
A7r2p2a4r0k4B0oulevard,
DuluthF,axMi(n2n1e8)so7t2a2525589121
August 10, 1998
Ms. Susan Beach 3M Environmental Technology and Safety Services 935 Bush Avenue Building 2-3E-09 POBox 33331. St. Paul, MN 55133-3331 -
Dear Sue:
FC-143 Daphnia magna reproduction not included during hypothesis testing
data for concentrations above the survival NOEC for the reproduction NOEC and LOEC. EPA
were
ignucilduadnecde isntihpyuploattehsestihsattecsotnicnegntfroartrieopnrsowduhcitcihons.ignTihfiecaenxtcllyuesfifoenctofsusruvcihvaclonscheonutlrdatniootnbs eusually
results in a more powerful and appropriate statistical analysis.
Sincerely, 7
Joe Dierkes Biologist, AScl
03838
FTiiltel:e: FC-143 DAPHfNc2I1AdMAGNA 21 DAY TRrEaPnRsOfDoUrCmT:ION ANOVA Table
NO TRANSFORMATION
SOURCE
oF
Between
4
Within (Error) 1s
Total
19
Critical F == 34..06595362 since F < Critical F
ss 115876.3000 399732.5000 5156088000
us 28969.0750 26648.8333
r 1.0871
((aallpphhaa == 00..0051,, ddff == 44,,1155))
(p-value = 0.3980)
FAIL TO REJECT Ho: All equal (alpha = 0.05)
Press any key to continue or ESC to return to menu...
93839
FTiiltel:e:
FC-143
DAPHfNc2I1AdMAGNA
21
DAY
REPRODUCTION Transform:
Dunnett's Test - TABLE 1 OF 2
NO TRANSFORMATION Ho:Control<Treatment
GROUP IDENTIFICATION
TRANSFORMED MEAN
MEOARNIGCIANLACLULUANTIETDS IN~~ T STAT s01.065
12 3
CONTROL 5 MG/L 8 MG/L
825.7500 722.5000 647.7500
825.7500 722.5000 647.7500
0.8945
4 5
13 MG/L 22 MG/L
630.5000 625.5000
630.5000 625.5000
11..56492105 1.7348
Dunnett critical value = 2.3600 (1 Tailed, alpha = 0.05, df = 4,15)
Press any key to continue...
Title: File:
FC-143
DAPHNIA MAGNA fe21d
21
DAY
REPRODUCTION Transform:
Dunnett's Test - TABLE 2 OF 2
NO TRANSFORMATION Ho:Control<Treatment
GROUP 1 2 a3 s
IDENTIFICATION
NUM OF REPS
CONTROL, a
5 MG/L 8 MG/L
4 4
13 MG/L 22 MG/L
4 4
MIN SIG (IN ORIG.
DIFF UNITS)
272.4182 227722..44118822 272.4182
* OF CONTROL
33.0 3333..00 33.0
FDRIOMFFECORNETNRCOEL 103.2500 117985..02050000 200.2500
Press any key to continue or ESC to return to menu... 03840
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
Shapiro - Wilk's Test forNormality
wb o- 399P7o32l.e5000
Critical W = 0.8680 (alpha = 0.01 , N = 20) W = 0.9050 (alpha = 0.05 , N = 20)
NO TRANSFORMATION
:
Data PASS normality test (alpha = 0.01). Continue analysis.
:
-
|!
aolrdo
k
woEe
Title:
File:
FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION Press any key to continue or ESC to return to menu...
fe21d
Transform:
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated 31 statistic = 6.7904
(p-value = 0.1474)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 = 9.4877
(alpha = 0.01, df = 4) (alpha = 0.05, df = 4)
Press any key to continue or ESC to return to memu...
93841
FTiiltel:e: FC-143 DAPHFCNLI4ADMAGNA 14 DAY TRrEaPnRsOfDoUrCmT:ION Dunnett's Test - TABLE 1 OF 2
NO TRANSFORMATION Ho:Control<Treatment
GROUP 21 13 5H 7
Dunnett
IDENTIFICATION
TRAMNSEFAONRMED
C5ONMTGR/OLL 138 MMGG//LL 3262 MMGG//LL 60 MG/L
331519..22550000 222684..57050000 129214..22550000 115.2500
critical value = 2.4600. (1 Tailed,
MEOARNIGCIANLACLULUANTIETDSI~N T STAT s0.1065
313159..22550000 222684..57050000 129214..22550000 115.2500
11..90803703 22..87342307 ++ 35.51225003 +*
alpha = 0.05, (aAfctu[aulseda]f == 66,,32210))
Press any key to continue...
FTiiltel:e: FC-143 DAPHFNCI1A4DMAGNA 14 DAYTRrEaPnRsOfDoUrCmT:ION
NO TRANSFORMATION
Dunnett's Test - TABLE 2 OF 2
Ho:Control<Treatment
GROUP IDENTIFICATION RNEUPMSOF (IMNINORISGI.G UDNIIFTFS) CO%NOTFROL FDRIOMFFECROENNTRCOEL
21 3
CO5NTMRGO/LL a4 8 MG/L 4
111177..22228866
33220.66 4984..05000000
15 6
213 MM6G//LL 44 36 MG/L 4
111177..22228866 117.2286
33220066 113350..07050000 32.6 168.0000
7
60 MG/L a
117.2286
32.6 244.0000
Press any key to continue or ESC to retu
03842
Title:
File:
FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
FC14D
Transform:
ANOVA Table
NO TRANSFORMATION
SouRCE
oF
Between
5
Within (Error)
21
Total
27
ss 152796.3571
95377.5000
248173.8571
us 25466.0595
4541.7857
5.6071
Critical F
5
= 3.8117 = 2.5727
.
(alpha = 0.01, df = 6,21) (alpha = 0.05, df = 6,21)
(p-value = 0.0013)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Press any key to continue or ESC to return to menu...
93843
FTiiltel:e: FC-143 DAPFHCN1I4ADMAGNA 14 DAY TRrEaPnRsOfDoUrCmT:ION Shapiro - Wilk's Test for Normality
WD e= 95037.79.453000 Critical WW == 00..58294600 ((aallpphhaa == 00..0051 |, NN == 2288))
NO TRANSFORMATION
Data PASS normality test (alpha = 0.01). Continue analysis.
.
.
1d repro
-
Aor
FTiiltel:e:
Press any key to continue or ESC to return to menu...
FC-143 DAPHFNCI1A4DMAGNA 14 DAY RTrEaPnRsOfDoUrCmT:ION
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic = 10.3740
(p-value = 0.1098)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B == 1162..85191169
((aallpphhaa
= =
00..0051,,
df df
= =
6) 6)
Press any key to continue or ESC to return to menu...
03844
;Tet
.
crow
1 2 3
45
6
FiCC--1/Y7%
Dctay Servi. vel
WoEE, LOES
Summary of Fisher's Exact Tests
ToEwTIEICATCIOoNton rEexvvo3ckn Doosn:e a&Tioss 5 MG/L
20
8 MG/L
20
13 MG/L
20
2326 MMGG//LL
2200
60 MG/L
20
1 4
4
45
-
16
*
bress any key to continue..
.
=
--
el
Srvia)
AoE
03845
Coe:
To O T
23
3
4
5 6
FC 143 11dswivea l oo JES, Le
Summary of Fisher's Exact Tests
--
= NUMBER
zz NUMBER
`CONTROL
20
0
85 mmgg//ll
2200
00
13 mg/l
20
0
22 mg/l
20
0
36 mg/l
20
0
60 mg/l
20
1
=SIG
-
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C3846
ENTER THE5 N2U0MB2E0R20OF2N5OR8T0ALITIES AT EACH CONCENTRATION:
WOULD YOU LIKE THE AUTOMATIC TRIM CALCULATION(Y/N)? Y
DATE: 8/7/1984 CHEMICAL: FC-143
TEST NUMBER: 1
DURATION: 21 DAYS SPECIES: D.MAGNA
RAW DATA:
M`ONCRUOTMNBACELERINTTERIXAEPTSOI:SOEND(:MG/L) SPEARMAN-KARBER TRIM:
5.i0o0os 208..d00o200o0% 13.1002000 22.i0g20o0
SPEARMAN-KARBER ESTIMATES: -- ECS0:
4a.57
9955%% ULPOPWEERR CCOONNFFIIDDEENNCCEE::
1480..6808
36.00a00s 60.100g00o
WOULD YOU LIKE TO HAVE A COPY SENT TO THE PRINTER (Y/N)?
i!
E22edn
03847
TTeosxticSatnatr/tEffDlautee:nt:8/7F/C1-918443 Test Ending Date: 8/21/198 TTeesstt SDpuercaiteiso:n: D.MAGNA 14 DAY DATA FILE:
coInDc. RepNluimcbaetres ConcentraMtGi/oLn
12
44
31
44
56
44
7
4
05..000000 138..000000 3262..000000 60.000
ReMsepaonnsse 313159..225500
222684..570500 219214..225500 115.250
stDdev.. 4350..375399 110171..905725 4481..057428 26.588
RespoPnosoeleMdeans 335191..225500 226248..755000 212941..225500 115.250
The Linear Interpolation Estimate: 39.6711 Entered P Value: 50
NThuembBeorotofstRreaspamEpsltiinmgast:es Me8a0n: Original Confidence Limits:
8039R.e1s7a1m0plSetsandGaernderDaetveidation: 6.7021 Lower: 27.6757 Upper: 48.1897
ERxepsaanmdpeldingContifmiedenicneSeLciomnidtss:: Low1e.r3:2 Ran2d0o.m47S8e4edU:ppe-r3:0905785534.3009
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ep
TTeosxticaSnttar/tEffDlauteen:t:8/7F/C1-918443 Test Ending Date: 8/28/84 Test Species: D.MAGNA
Keo 190
Test Duration:
21 pay
au
DATA FILE:
CoInDe.
RNepulmibceartes
Concentration MG/L
ReMsepaonnsse
sDetva.. RespoPnosoeledMeans
21
44
a3
44
65
44
7
4
05..000000 138..000000 2326..000000 60.000
872252..755000 663407..570500 562235..500000 155.250
14635..431823 221422..181611 12696..955566 35.753
872252..755000 663407..570500 562235..050000 155.250
The Linear Interpolation Estimate: 43.1869 Entered B Value: 50
NThuembBeorotosftrRaespamEpsltiinmgast:es Me8a0n: Original Confidence Limits:
8042.R7e2s2a6mplSetsanGdaernderDaetveidation: 2.9224 Lower: 35.0020 Upper: 46.3843
REexspaamnpdleidngContfimiedenicneSeLciomnidtss:: Low1e.r2:6 _Ran3d0o.m0S91e0edU:ppe-r5:2384864984.2386
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03848