Document 7QOxZej4kdYN5qdm74Evb8Gj
AR226-3169
TRADE SECRET
Study Tide H-24615: Static, Acute, 96-Hour Screening Test
with Pimephales promelas Laboratory Project ID: DuPont-5019
AUTHOR: Robert A. Hoke, Ph.D. STUDY COMPLETED ON: October 10,2000
PERFORMING LABORATORY:
E.I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine
Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
WORK REQUEST NUMBERi^fl
STUDY CODE NUMBER:|^^P
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H-24615: Static, Acute, 96-Hcmr Screenmg Test to Pimephales promelas
DuPont-5019
STUDY INFORMATION
Substance Tested: Synonyms/Codes:
Haskell Number: Composition:
Known Impurities: Physical Characteristics:
Stability:
Sponsor:
m-Life Initiated/Completed:
Not supplied by the sponsor
Milky to tan liquid
The test substance appeared to be stable under the
conditions of the study.
E.I. du Font de Nemours and Company Wilmington, Delaware 19898 U.S.A.
September 25,2000/September 29,2000
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H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-5019
SUMMARY
The acute toxicity of H-24615 to the fathead minnow, Pimephales promelas was determined in
an unaerated, 96-hour, static test.
The study was conducted with 4 concentrations of H-24615 and a dilution water control at a mean temperature of 21.5C. One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1,10 and 100 mg/L test concentrations were clear and colorless at test start. The 1000 mg/L test concentration was a cloudy, whitish/tan color at test start. All water quality parameters were within acceptable limits during the test.
Exposure of fathead minnows to the water control and nominal H-24615 concentrations of 1,10,100, and 1000 mg/L resulted in 0,0,0,0, and 100% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The
highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest mean, nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24615 concentrations were used to calculate the LC5o value using the binomial procedure.
The results are summarized as follows:
Nominal concentrations of H-24615, mg/L 96-hour LCso (95% confidence interval)
for H-24615, based on nominal concentrations, mg/L"
dilution water control, 1,10,100, and 1000 316 (100 to 1000)
H-24615 exhibited low concern for aquatic hazard a in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas.
Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTMSTP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia.
---- Qoapacy seaSeos Does not contain TSCA CBI
H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-5019
CERTIFICATION
We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.
Work Completed by:
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Darnel J.C. Peterson
Associate Scientist
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Issued by Study Director:
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Senior Research Scientist
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Company Sanitized. Does not contain TSCA CBI
H-24615: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-5019
TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-24615 Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
PH
Test Completion (96 hours)
Dissolved
Oxygen
(mg/L)
PH
Water Control
8.7
7.5
6.4
7.3
1
8.7
7.5
5.3
7.3
10
8.7
7.5
5.8
7.3
100
8.7
7.5
5.2
7.3
1000
8.7
7.5
7.4s
7.42
a Measurement taken at 48 hours due to total mortality
TABLE 2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR
TEST WITH H-24615
Nominal H-24615 Concentration (mg/L)
Water Control
1 10 100 1000
Number Dead/ Number at Test Initiation
24 Hours
48 Hours
72 Hours
96 Hours
0/10 0/10 0/10 0/10 10/10
0/10
0/10
0/10
0/10
,
10/10
0/10 0/10 0/10 0/10 10/10
0/10 0/10 0/10 0/10 10/10
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